Organisms | Microscopy | Staining | Fluorescent microscopy | ELISA | ||||
---|---|---|---|---|---|---|---|---|
 | Direct | After concentration | Saffranin | Acid Fast | Autoflourescence | Calcoflour White | Calcoflour White + DAPI |  |
Cryptosporidium spp. | ||||||||
Sensitivity | 63.19% | 74.23% | 83.44% | 90.79% | - | - | - | 92.02% |
Specificity | 93.03% | 95.82% | 98.26% | 97.91% | - | - | - | 96.12% |
PPV | 83.74% | 90.98% | 96.45% | 96.1% | - | - | - | 97.4% |
NPV | 81.65% | 86.75% | 91.26% | 94.93% | - | - | - | 88.39% |
Microsporidia spp. | ||||||||
Sensitivity | - | - | - | - | - | 95.19% | 97.12% | - |
Specificity | - | - | - | - | - | 97.69% | 98.55% | - |
PPV | - | - | - | - | - | 92.52% | 95.28% | - |
NPV | - | - | - | - | - | 98.54% | 99.13% | - |
Cyclospora spp. | ||||||||
Sensitivity | 65.22% | 78.26% | 89.13% | 85.87% | 97.83% | - | - | - |
Specificity | 97.21% | 98.04% | 99.16% | 98.6% | 100% | - | - | - |
PPV | 85.71% | 91.14% | 96.47% | 94.05% | 100% | - | - | - |
NPV | 91.58% | 94.61% | 97.26% | 96.45% | 99.44% | - | - | - |