Table 2 Bacterial strains and plasmids used in this study.
Characteristics | Reference | |
|---|---|---|
Strains | ||
Brucella abortus | ||
2308 | Virulent laboratory strain | |
2308ΔureTp | 2308 ureT polar mutant | This work |
2308ΔureT | 2308 ureT non-polar mutant | This work |
2308ΔnikO | 2308 nikO non-polar mutant | This work |
Escherichia coli | ||
DH5α | Standard E. coli cloning strain | [36] |
S17-1 λ pir | Mobilizing donor for conjugation | [37] |
Plasmids | ||
pGEM-T Easy | PCR cloning vector | Promega |
pRH016 | Gateway shuttle vector | [31] |
pDS132 | Suicide mobilizable plasmid | [38] |
pBBR1 MCS | Broad-host-range plasmid | [39] |
pFJS225 | U_ureT in pGEMT-Easy | This work |
pFJS226 | D_ureT in pGEMT-Easy | This work |
pFJS227b | ΔureT::aph in pDS132 | This work |
pFJS235 | pUC4K with aphT | This work |
pFJS236 | ΔureT in pGEMT-Easy | This work |
pFJS237 | ΔnikO in pGEMT-Easy | This work |
pFJS238 | ΔureT::aphT in pGEMT-Easy | This work |
pFJS239 | ΔnikO::aphT in pGEMT-Easy | This work |
pFJS241b | ΔureT::aphT in pDS132 | This work |
pFJS242b | ΔnikO::aphT in pDS132 | This work |
pFJS243 | ureT in pRH016 | This work |
pFJS244 | nikO in pGEMT-Easy | This work |
pFJS245 | nikO in pBBR1 MCS | This work |