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Table 2 Differentially expressed flagellar genes in the HP0256 mutant.

From: The HP0256 gene product is involved in motility and cell envelope architecture of Helicobacter pylori

Proposed Class TIGR orf no. Putative gene product (gene) Expression ratio p-value
Class I HP0019 chemotaxis protein (cheV) 1.221 0.026
  HP0082 methyl-accepting chemotaxis transducer (tlpC) 0.945 0.378
  HP0099 methyl-accepting chemotaxis protein (tlpA) 1.401** 0.112
  HP0103 methyl-accepting chemotaxis protein (tlpB) 1.403** 0.05
  HP0173 flagellar biosynthetic protein (fliR) 1.000 0.997
  HP0244 signal-transducing protein, histidine kinase (atoS) 1.221 0.651
  HP0246 flagellar basal-body P-ring protein (flgI) - -
  HP0325 flagellar basal-body L-ring protein (flgH) 1.113 0.050
  HP0326 CMP-N-acetylneuraminic acid synthetase (neuA) 0.904 0.219
  HP0327 flagellar protein G (flaG) 0.749 0.238
  HP0351 basal body M-ring protein (fliF) 0.889 0.508
  HP0352 flagellar motor switch protein (fliG) 1.158 0.176
  HP0391 purine-binding chemotaxis protein (cheW) 1.668** 0.004
  HP0392 histidine kinase (cheA) 1.202 0.113
  HP0393 chemotaxis protein (cheV) 1.176 0.194
  HP0584 flagellar motor switch protein (fliN) 1.758** 0.042
  HP0599 hemolysin secretion protein precursor (hylB) 1.201 0.366
  HP0616 chemotaxis protein (cheV) 1.159** 0.162
  HP0684 flagellar biosynthesis protein (fliP) 0.510 0.058
  HP0685 flagellar biosynthetic protein (fliP) 0.493 0.066
  HP0703 response regulator 0.715 0.158
  HP0714 RNA polymerase sigma-54 factor (rpoN) 1.104 0.699
  HP0770 flagellar biosynthetic protein (flhB) 0.621 0.162
  HP0815 flagellar motor rotation protein (motA) 0.917 0.538
  HP0816 flagellar motor rotation protein (motB) 0.651 0.231
  HP0840 flaA1 protein 1.296 0.184
  HP1041 flagellar biosynthesis protein (flhA) 0.988 0.921
  HP1067 chemotaxis protein (cheY) 0.958 0.905
  HP1092 flagellar basal-body rod protein (flgG) 1.142 0.140
  HP1286 conserved hypothetical secreted protein (fliZ) 1.305 0.544
  HP1419 flagellar biosynthetic protein (fliQ) 0.636 0.036
  HP1420 flagellar export protein ATP synthase (fliI) 0.687 0.012
  HP1462 secreted protein involved in flagellar motility 1.306 0.003
  HP1575 homolog of FlhB protein (flhB2) 1.445 0.239
  HP1585 flagellar basal-body rod protein (flgG) 0.590 0.019
Class II HP0114 hypothetical protein 1.230 0.357
  HP0115 flagellin B (flaB) 1.906 0.032
  HP0295 flagellin B homolog (fla) 1.734 0.179
  HP0869 hydrogenase expression/formation protein (hypA) 1.307 0.109
  HP0870 flagellar hook (flgE) 1.892* 0.067
  HP0906 hook length control regulator (fliK) 1.13** 0.230
  HP1076 hypothetical protein 2.595 0.001
  HP1119 flagellar hook-associated protein 1 (HAP1) (flgK) 1.300 0.224
  HP1120 hypothetical protein 1.199 0.390
  HP1154 hypothetical protein (operon with murG) 1.514 0.055
  HP1155 transferase, peptidoglycan synthesis (murG) 1.955 0.034
  HP1233 putative flagellar muramidase (flgJ) 1.400 0.144
Class III HP0472 outer membrane protein (omp11) 1.649 0.009
  HP0601 flagellin A (flaA) 1.487 0.229
  HP1051 hypothetical protein 1.098 0.501
  HP1052 UDP-3-0-acyl N-acetylglucosamine deacetylase (envA) 1.648 0.054
Intermediate HP0165 hypothetical protein 1.226 0.515
  HP0166 response regulator (ompR) 1.596 0.057
  HP0366 spore coat polysaccharide biosynthesis protein C 0.860 0.419
  HP0367 hypothetical protein 1.853 0.008
  HP0488 hypothetical protein 0.711** 0.031
  HP0907 hook assembly protein, flagella (flgD) 1.271 0.214
  HP0908 flagellar hook (flgE) 1.175 0.119
  HP1028 hypothetical protein 0.852 0.286
  HP1029 hypothetical protein 0.799 0.019
  HP1030 fliY protein (fliY) 0.860** 0.308
  HP1031 flagellar motor switch protein (fliM) 0.835 0.054
  HP1032 alternative transcription initiation factor, sigma28 (fliA) 0.923 0.371
  HP1033 hypothetical protein 0.896 0.467
  HP1034 ATP-binding protein (ylxH) 0.87** 0.352
  HP1035 flagellar biosynthesis protein (flhF) 0.921 0.187
  HP1122 anti-sigma 28 factor (flgM) 0.867 0.310
  HP1440 hypothetical protein 0.627 0.026
  HP1557 flagellar basal-body protein (fliE) 0.652 0.091
  HP1558 flagellar basal-body rod protein (flgC) (proximal rod protein) 0.899 0.480
  HP1559 flagellar basal-body rod protein (flgB) (proximal rod protein) 1.305 0.194
  HP0751 (flaG2) 1.203 0.350
  HP0752 flagellar cap protein (fliD) 1.003 0.986
  HP0753 flagellar chaperone (fliS) 0.981 0.825
  HP0754 flagellar chaperone (fliT) 1.09** 0.400
Not assigned HP0410 flagellar sheath associated protein (hpaA2) 0.664 0.038
  HP0492 flagellar sheath associated protein (hpaA3) 0.256 0.000
  HP0797 flagellar sheath associated protein (hpaA) 0.801 0.170
  1. Full array datasets are in public databases as described in Methods. Fold-changes and p-values were calculated based on 4 independent biological replicates as described in Methods. Open reading frames and gene annotations were based on the TIGR database [23]. The genes were classified in different flagellar classes, as previously proposed [8]. Confirmatory analysis by qRT-PCR was performed for genes with *. Values for genes with ** were lost during the initial array data analysis and subsequently recovered using 3 independent replicates. For technical reasons, some array spots could not be analyzed in individual arrays.