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Figure 7 | BMC Microbiology

Figure 7

From: Functional dissection of translocon proteins of the Salmonella Pathogenicity Island 2-encoded type III secretion system

Figure 7

Effect of mutations in SseB or SseD on translocation of the SPI2 effector protein SseJ. Macrophages were infected at a MOI of 10 with S. Typhimurium wild type (WT), sseB, sseB [psseB] or sseB harboring plasmids for expression of various sseB mutant alleles (sseB [psseB Δx]) (A), or WT, sseD, sseD [psseD], or various strains harboring chromosomal deletion in sseD (B). All strains harbored a chromosomal translational fusion of the firefly luciferase to codon 200 of sseJ. At 8 h (B) or 14 h (A) post infection, the host cells were lysed and the numbers of intracellular bacteria were determined. The rest of the cell lysates were centrifuged and the luciferase activity (relative light units = RLU) was measured in the supernatant in order to quantify the translocation of SseJ-Luc. The RLU per bacterium were calculated to compensate different replication rates of WT and the sseB mutant strains. Means and standard deviations of triplicate assays are shown and all experiments were performed at least twice.

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