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Table 5 Sense and antisense primers for real-time PCR

From: Virulence-related Mycobacterium avium subsp hominissuis MAV_2928 gene is associated with vacuole remodeling in macrophages

Target

Primers

PCR product (bp)

β-actin

5'-TGATGGTGGGCATGGGTCAGA-3'

5'-CCCATGCCAATCTCATCTTGT-3'

800

GRK4

5'-AATGTATGCCTGCAAAAAGC-3'

5'-GATTGCCCAGGTTGTAAATG-3'

235

DGKD

5'-CTCGGCTTACGGTTATTCCAG-3'

5'-CCATCTCCATCTTCAGCCTCC-3'

656

LCP2

5'-CACTGAGGAATGTGCCCTTTC-3'

5'-GTGCCTCTTCCTCCTCATTGG-3'

408

  1. Complemented 2D6 mutant had similar results to the wild-type bacterium. Y = Yes; N = No
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BMC Microbiology

ISSN: 1471-2180

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