Skip to main content

Table 5 Sense and antisense primers for real-time PCR

From: Virulence-related Mycobacterium avium subsp hominissuis MAV_2928 gene is associated with vacuole remodeling in macrophages

Target Primers PCR product (bp)
β-actin 5'-TGATGGTGGGCATGGGTCAGA-3'
5'-CCCATGCCAATCTCATCTTGT-3'
800
GRK4 5'-AATGTATGCCTGCAAAAAGC-3'
5'-GATTGCCCAGGTTGTAAATG-3'
235
DGKD 5'-CTCGGCTTACGGTTATTCCAG-3'
5'-CCATCTCCATCTTCAGCCTCC-3'
656
LCP2 5'-CACTGAGGAATGTGCCCTTTC-3'
5'-GTGCCTCTTCCTCCTCATTGG-3'
408
  1. Complemented 2D6 mutant had similar results to the wild-type bacterium. Y = Yes; N = No