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Table 1 Bacterial strains and plasmids used

From: A glutamine-amidotransferase-like protein modulates FixT anti-kinase activity in Sinorhizobium meliloti

Material Characteristics References
Strains Genotype  
S. meliloti   
GMI211 SmR Nod+ Fix+ [20]
GMI401 GMI211 asnO::Tn5 SmR NmR This work
GMI5704 GMI211 fixJ2.3::Tn5 SmR NmR BleoR [18]
E. coli   
ER asnA31 asnB32 thi [15]
MM294 Pro-82 thi-1 hsdR17 supE44 endA1 [21]
DH5 α endA1 hsdR17 (rk-mk+) supE44 thi-1 recA1 gyrA relA1Δ (lacIZYA- [29]
  argF)U169 deoR (φ 80dlacΔ (lacZ)M15)  
Plasmids   
pMF457 pGD926 (IncP broad host range vector) derivative carrying a fixK-  
  lacZ fusion with a mutation in the fixT promoter. TcR  
pCHK57 pGMI41211 (Inc-P1 broad host range vector) derivative carrying a [31]
  nifA-lacZ fusion. TcR  
pMF11 Same as pMF10 but with fixT in reverse orientation. GmR  
pBH1 pBBR1-MCS3 derivative, carrying an HindIII fragment containing This work
  the asnO gene and the SMb20482 orf.  
pBasn2 pBBR1-MCS3 derivative, expressing asnO under the control of the This work
  pLac promoter  
pUC23 pUC18 digested with EcoRI, carrying the S. meliloti EcoRI This work
  fragment containing the asn O region with the Tn5 insert. ApR,  
  KmR.  
BAC37 pBeloBAC11 containing a 100 kb S. meliloti genome fragment [26]
  carrying the asn-like region of pSymB.  
pRK602 PRK600::Tn5, Cmr, Nm-Kmr [21]