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Figure 5 | BMC Microbiology

Figure 5

From: A glutamine-amidotransferase-like protein modulates FixT anti-kinase activity in Sinorhizobium meliloti

Figure 5

asnO gene expression RT-PCR analysis of RNAs isolated from S. meliloti GMI211 wild-type strain (wt) or GMI5704 fixJ mutant strain ( fixJ ) grown in minimal medium M9 in either oxic ( + ) or microoxic (2% O2) conditions ( - ). RT-PCR were performed with either asnO or hemA specific primers and the products were separated on agarose gels, blotted on a nylon membrane and hybridized with the corresponding 32P labelled PCR product and analysed on a Phosphorimager.

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