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Figure 2 | BMC Microbiology

Figure 2

From: Phenotypic silencing of cytoplasmic genes using sequence-specific double-stranded short interfering RNA and its application in the reverse genetics of wild type negative-strand RNA viruses

Figure 2

Effect of dsRNA on the cell fusion activity of RSV. A549 monolayers were transfected with 20 nM of anti-P or anti-F dsRNA and infected with RSV as described under Materials and Methods. At 40 h p.i., the monolayers were examined under a Nikon TS100F phase-contrast microscope at 40× magnification and digitally photographed with a Nikon Coolpix 995 camera. Note the syncytia in 'A', cytopathic effect without syncytia in 'B', and monolayers that appear unaffected and identical in 'C' and 'D'.

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