Figure 5

Virulence of wild type isolate CS101 (square) and the isogenic β-hemolysis negative sagA/pel mutant, CS101 sagA/pel::Tn917 (circle) and a β-hemolytic kidney recovered CS101 pel::Tn917 KR sagA/pel mutant variant (triangle). Groups of 10 outbred CD1 mice were injected with 1 × 109 cfu into a skin air sack. Time to death was monitored and statistical significance was determined by use of Student's t test (wt vs. sagA/pel::Tn917 p = 0.54; sagA/pel::Tn917 vs. KR p = 0.018).