Skip to main content

Advertisement

Figure 4 | BMC Microbiology

Figure 4

From: Mouse skin passage of a Streptococcus pyogenes Tn917 mutant of sagA/pel restores virulence, beta-hemolysis and sagA/pel expression without altering the position or sequence of the transposon

Figure 4

Phenotypes of CS101 wt, CS101 sagA/pel::Tn917 and CS101 sagA/pel::Tn917 kidney-recovered (KR) variants. Panel A. Bacterial binding of 125I labeled fibrinogen CS101 wt (square), CS101 sagA/pel:: Tn917 (circle) and CS101 sagA/pel Tn917 kidney-recovered (triangle). Panel B. Streptococcal pyogenic exotoxin B (SpeB) activity of culture supernatants treated with DTT. CS101 wt (square), CS101 sagA/pel::Tn917 (circle) and CS101 sagA/pel::Tn917 kidney-recovered (triangle). Panel C. Streptokinase (SK) activity of culture supernatants grown in the presence of cysteine protease inhibitor E 64 (10 μM) to prevent destruction of SK by any secreted cysteine protease; CS101 wt (square), CS101 sagA/pel::Tn917 (circle) and CS101 sagA/pel::Tn917 KR (triangle).

Back to article page