Figure 3

Bacterial expression of recombinant PfPP5. Soluble S100 extract (approximately 30 μg protein) of IPTG-induced (lanes 2, 4) or uninduced (lanes 1, 3) E. coli BL21(DE3)-RIG containing pET-15b-PfPP5 (lanes 1, 2) or pET-15b-ΔTPR-PfPP5 (lanes 3, 4) were analyzed by SDS-PAGE followed by staining of the gel with Coomassie Brilliant Blue R250 [12]. The respective purified proteins were analyzed in lanes 5, 6. Lane 7 represents about 5 μg of purified His-tagged PfPP5 digested with 50 ng of trypsin for 30 min at 30°. Protein markers (lane M) are indicated by their Mr in thousands.