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Figure 2 | BMC Microbiology

Figure 2

From: Characterization of Norwalk virus GI specific monoclonal antibodies generated against Escherichia coli expressed capsid protein and the reactivity of two broadly reactive monoclonal antibodies generated against GII capsid towards GI recombinant fragments

Figure 2

Expression of the rNV96-908 fragments in E. coli. Production of TRX-fused capsid protein fragments in E. coli was analyzed by staining two SDS-PAGE gels. From the left, lane 1 and lane 21 : marker [kDa], lane 2 and lane 2: control recombinant E. coli lysate (before induction). From lane 3 to 23 except 21 and 22 are TRX-NV96-908 N-terminal domain, C-terminal domain, fragment 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, and 17, respectively. The positions of the recombinant NV capsid fragments are indicated by arrows (the small fragments show similar molecular weights).

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