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Table I Y. pseudotuberculosis proliferation in various conditions

From: The Yersinia YopE and YopH type III effector proteins enhance bacterial proliferation following contact with eukaryotic cells

Experiment #1 a
  Media Cell-associated
Time point   Fold-increase   (± S.D.)
(hour) Cfu/well (± S.D.) Cfu/well (± S.D.)
  13.0 × 104   968  
0 4.8 × 104 1.0 1064 1.0
  4.9 × 104   654  
  7.4 × 105   5150  
4 6.6 × 105 8.6(1.1) 4640 4.9 (0.3)
  5.8 × 105   4320  
  2.7 × 107   78000  
8 2.1 × 107 293 (54) 60800 75 (10)
  1.9 × 107    62800
Experiment #2 b
  Cell-associated
  -Cytochalasin D +Cytochalasin D
Time point   Fold-increase   Fold-increase
(hour) Cfu/well (± S.D.) Cfu/well (± S.D.)
  476   656  
0 503 1.0 768 1.0
  432   544  
  13200   50400  
6 20000 39(9) 52000 70 (14)
  21200   35200  
  1. Y. pseudotuberculosis was added to wells containing either tissue culture media alone or cultured RAW cells (MOI = 0.2). Shown is either the titer of the wells containing tissue culture media alone (media) or the number of bacteria recovered from wells containing RAW cells (cell-associated). Three independent wells for each condition were assayed and the fold-increases are indicated (± sample standard deviation). b. Similar infection as in Experiment #1 except that some cells were treated with 10 μg/ml cytochalasin D 30 minutes prior to the addition of the bacteria.