Experiment #1
a
|
---|
| Media | Cell-associated |
---|
Time point | | Fold-increase | | (± S.D.) |
---|
(hour) | Cfu/well | (± S.D.) | Cfu/well | (± S.D.) |
---|
| 13.0 × 104 | | 968 | |
0 | 4.8 × 104 | 1.0 | 1064 | 1.0 |
| 4.9 × 104 | | 654 | |
| 7.4 × 105 | | 5150 | |
4 | 6.6 × 105 | 8.6(1.1) | 4640 | 4.9 (0.3) |
| 5.8 × 105 | | 4320 | |
| 2.7 × 107 | | 78000 | |
8 | 2.1 × 107 | 293 (54) | 60800 | 75 (10) |
| 1.9 × 107 | | | 62800 |
Experiment #2
b
|
| Cell-associated |
| -Cytochalasin D | +Cytochalasin D |
Time point | | Fold-increase | | Fold-increase |
(hour) | Cfu/well | (± S.D.) | Cfu/well | (± S.D.) |
| 476 | | 656 | |
0 | 503 | 1.0 | 768 | 1.0 |
| 432 | | 544 | |
| 13200 | | 50400 | |
6 | 20000 | 39(9) | 52000 | 70 (14) |
| 21200 | | 35200 | |
- Y. pseudotuberculosis was added to wells containing either tissue culture media alone or cultured RAW cells (MOI = 0.2). Shown is either the titer of the wells containing tissue culture media alone (media) or the number of bacteria recovered from wells containing RAW cells (cell-associated). Three independent wells for each condition were assayed and the fold-increases are indicated (± sample standard deviation). b. Similar infection as in Experiment #1 except that some cells were treated with 10 μg/ml cytochalasin D 30 minutes prior to the addition of the bacteria.