amiA is a negative regulator of acetamidase expression in Mycobacterium smegmatis

BMC Microbiology

Table 2 Promoter activity assays.

		Wild-type	Wild-type	Mad1	Mad1
Construct	Promoter	MM-S	MM-AS	MM-S	MM-AS
pAGAN120	P₂	110 ± 34	169 ± 40	332 ± 110	301 ± 256
pAGAN130	P₁	705 ± 151	671 ± 109	970 ± 278	1079 ± 444
pAGAN131	P_c	20 ± 2.4	22 ± 6.8	41 ± 17	86 ± 46
pAGAN170	P₃	2.4 ± 0.5	4.1 ± 1.2	nd	nd
pJEM control	none	0.8 ± 0.4	1.3 ± 0.1	1.3 ± 0.2	2.2 ± 1.5

The constructs from figure 3 were assayed for β-galactosidase activity in both the wild-type and Mad1 strains. Values represent the mean ± standard error of six values from three independent transformants and are expressed as nmol O-nitro phenyl galactoside produced per min per mg total protein. Constructs pAGAN121, 160, 161 and 171 were all negative. nd – not determined.

ISSN: 1471-2180