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Table 2 Promoter activity assays.

From: amiA is a negative regulator of acetamidase expression in Mycobacterium smegmatis

   Wild-type Wild-type Mad1 Mad1
Construct Promoter MM-S MM-AS MM-S MM-AS
pAGAN120 P2 110 ± 34 169 ± 40 332 ± 110 301 ± 256
pAGAN130 P1 705 ± 151 671 ± 109 970 ± 278 1079 ± 444
pAGAN131 Pc 20 ± 2.4 22 ± 6.8 41 ± 17 86 ± 46
pAGAN170 P3 2.4 ± 0.5 4.1 ± 1.2 nd nd
pJEM control none 0.8 ± 0.4 1.3 ± 0.1 1.3 ± 0.2 2.2 ± 1.5
  1. The constructs from figure 3 were assayed for β-galactosidase activity in both the wild-type and Mad1 strains. Values represent the mean ± standard error of six values from three independent transformants and are expressed as nmol O-nitro phenyl galactoside produced per min per mg total protein. Constructs pAGAN121, 160, 161 and 171 were all negative. nd – not determined.