Acetamidase expression in Mad1. Cell-free extracts were electrophoresed though a 10 % acrylamide gel. Lanes are 1: wild-type MM-S 2: Wild-type MM-AS 3: Mad1 MM-S 4: Mad1 MM-AS 5: Mad1/pAGAN303 MM-S 6: Mad1/pAGAN303 MM-AS 7: markers, size given in kDa. The acetamidase band at 47 kDa is indicated. MM-S: minimal media with succinate. MM-AS: minimal media with acetamide and succinate.