Vector constructs. A: Arrangement of genes in the Mycobacterium smegmatis chromosome. amiA, amiC and amiD are proposed regulators, amiS encodes one component of a putative ABC transporter. amiE is the acetamidase gene. B: The two fragments used to generate the delivery vector for construction of the amiA deletion mutant. The resulting SalI deletion in the Mad1 strain is indicated which removes the majority of the amiA gene. C: The region used to complement the Mad1 mutant.