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Table 2 Bactericidal and Opsonophagocytic Activity of monoclonal antibodies raised against IROMPs of A. baumannii. The antibodies were tested for both type of bacteria i.e. bacteria grown in CDM-Fe and CDM+Fe medium. Results are the mean of three independent experiments in both the cases. ± represents the standard deviation.

From: Monoclonal antibodies against the iron regulated outer membrane Proteins of Acinetobacter baumannii are bactericidal

Antibodies/

Bactericidal

Bactericidal

No. of Bacteria

No. of Bacteria

Various Controls

Activity in

Activity in

grown in CDM-

grown in CDM-

 

CDM-Fe

CDM+Fe

Fe medium per

Fe medium per

 

medium(%)

medium(%)

PMN

PMN

2G9ABIR

98.0% ± 8.0%

15.0% ± 4%

9.0 ± 2.0

2.0 ± 1.0

3D5ABIR

97.0% ± 12.0%

22.0% ± 9%

11.0 ± 3.5

2.0 ± 0.5

5D6ABIR

90.0% ± 11.0%

9.00% ± 4%

10.5 ± 2.5

1.0 ± 0.5

1D11ABIR

97.0% ± 17%

19.00% ± 6%

9.0 ± 1.5

3.0 ± 1.0

1F7ABIR+

93.0% ± 5%

26.0% ± 12%

10.0 ± 1.5

3.0 ± 1.8

Preimmune Sera

12.0% ± 2%

16.0% ± 6%

1.5 ± 0.5

2.5 ± 1.0

Complement only

12.0% ± 5%

8.50% ± 1%

-

-

Tissue culture

24.0% ± 7%

21.0% ± 4%

2.0 ± 0.5

1.5 ± 0.2

supernatant only

    

Polyclonal Sera

68.0% ± 13%

39.0% ± 7%

7.0 ± 1.0

4.5 ± 1.0

Only PBS

1.0% ± 1%

2.0% ± 1.5%

1 ± 0.2

1 ± 0.5