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Table 1 Characterisation of monoclonal antibodies. The antibodies were tested by ELISA using OMPs from both CDM-Fe and CDM+Fe grown bacteria. The antibody which shows 1.0 or more than 1.O OD value with CDM-Fe OMPs were selected as an antibody against IROMPs of A. baumannii and used for subsequent experiments.

From: Monoclonal antibodies against the iron regulated outer membrane Proteins of Acinetobacter baumannii are bactericidal

Monoclonal

ELISA

Ig Isotype

Titre in ELISA

Antibodies

Fe-

Fe+

  

2G9ABIR

2.762

.141

IgM

1:100

3D5ABIR

2.722

.135

IgM

1:3200

5D6ABIR

3.093

.562

IgM

1:100

1D11ABIR

2.925

.615

IgM

1:8000

1F7ABIR

2.975

.412

IgM

1:800

Preimmune sera

.452

.458

-

1:200