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Table 3 Bacterial strains and plasmids used or created in this study.

From: MtnK, methylthioribose kinase, is a starvation-induced protein in Bacillus subtilis

Strain or plasmid Genotype or description Source or reference
Strains   
Escherichia coli   
XL1-Blue K12 supE 44 hsdR 17 recA 1 endA 1 gyrA 46 thi Laboratory collection
  relA 1 lac- F' [proAB+lacIqlacZ Δ M15 Tn10(tetR)]  
Bacillus subtilis   
168 trpC 2 [36]
BSIP7004 trpC 2 speD::spc [2]
BFS1850 trpC 2mtnK::lacZ This worka
BFS1851 trpC 2mtnU::lacZ This worka
BSHP1850 trpC 2speD::spc mtnT::lacZ This work
BSHP7010 trpC 2 mtnS::spc This work
BSHP7035 trpC 2pmtnK::Tn10 This work
Plasmids   
pUC19 cloning vector, AmpR [37]
pIC333 mini-Tn10 delivery vector, SpcR, EryR [32]
pMutin4mcs cloning vector, ErmR [31]
pDU1850 pMutin4mcs::mtnK This work
pDU1851 pMutin4mcs::mtnU This work
pHPP7010 pUC19mtnS::spcb This work
  1. a. This strain has been constructed during the frame of the EC project for the functional characterization of the genome of B. subtilis in Europe b. spc is the spectinomycin resistance gene from Staphylococcus aureus