MtnK, methylthioribose kinase, is a starvation-induced protein in Bacillus subtilis

BMC Microbiology

Table 3 Bacterial strains and plasmids used or created in this study.

Strain or plasmid	Genotype or description	Source or reference
Strains
Escherichia coli
XL1-Blue	K12 supE 44 hsdR 17 recA 1 endA 1 gyrA 46 thi	Laboratory collection
	relA 1 lac^- F' [proAB⁺lacI^qlacZ Δ M15 Tn10(tet^R)]
Bacillus subtilis
168	trpC 2	[36]
BSIP7004	trpC 2 speD::spc	[2]
BFS1850	trpC 2mtnK::lacZ	This work^a
BFS1851	trpC 2mtnU::lacZ	This work^a
BSHP1850	trpC 2speD::spc mtnT::lacZ	This work
BSHP7010	trpC 2 mtnS::spc	This work
BSHP7035	trpC 2pmtnK::Tn10	This work
Plasmids
pUC19	cloning vector, Amp^R	[37]
pIC333	mini-Tn10 delivery vector, Spc^R, Ery^R	[32]
pMutin4mcs	cloning vector, Erm^R	[31]
pDU1850	pMutin4mcs::mtnK	This work
pDU1851	pMutin4mcs::mtnU	This work
pHPP7010	pUC19mtnS::spc^b	This work

a. This strain has been constructed during the frame of the EC project for the functional characterization of the genome of B. subtilis in Europe b. spc is the spectinomycin resistance gene from Staphylococcus aureus

ISSN: 1471-2180