MtnK, methylthioribose kinase, is a starvation-induced protein in Bacillus subtilis

Table 1 Expression of mtnK::lacZ transcriptional fusions.

		β-galactosidase Activity (U mg^-1 of protein)
Medium used^a	Strain	No MTR added	MTR added
ED1 minimal medium	BFS1850^b	1550	1650
methionine	BFS1850	1275	1250
isethionate	BFS1850	1800	1700
coenzymeM	BFS1850	1400	1370
taurine^$	BFS1850	5000	4600
proline^$	BFS1850	2800	2600
xylose + ammonium^$	BFS1850	6500	ND
xylose^$	BFS1850	3400	ND
ED1 minimal medium	BSHP1850^c	1660	1700
methionine	BSHP1850	1350	1300

a. for the β-galactosidase activity assay the bacteria were grown in the ED minimal medium as standard conditions. When another one replaced one of these glutamine, glucose and MgSO₄ components, only that change is indicated (for concentrations see Materials and Methods). b. BFS1850 = mtnK::lacZ c. BSHP1850 = speD::spc mtnK::lacZ $. starvation conditions