Gastroenteritis outbreaks associated with Norwalk-like viruses and their investigation by nested RT-PCR

BMC Microbiology

Table 1 NLV primers and references

Primer		Primer Sequence	Primer	Reference
A^*	Outer +	5'-ATA CCA CTA TGA TGC AGA TTA-3'	JV12	[3]
B	Outer-	5'-CGA TTT CAT CAT CAC CAT A-3'	SM31	[18]
C	Inner +	5'-GAA TTC CAT CGC CCA CTG GCT-3'	N1	[17]
D	Inner-	5'-ATC TCA TCA TCA CCA TA-3'	E3	[17]

First round primers A+B yielded a product of 333 bp. Second round nested primers C+D yielded a product of 114 bp.

ISSN: 1471-2180