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Figure 4 | BMC Microbiology

Figure 4

From: Evidence that the RNAseH activity of the duck hepatitis B virus is unable to act on exogenous substrates

Figure 4

RNAseH assay using micrococcal nuclease treated cores after permeabilization. Wild type viral core particles (DHBV) or reverse transcriptase-activity deficient core particles (DHBV-YMHA) were permeabilized and the nucleic acids within the cores were either digested with micrococcal nuclease (lanes 3-6), or a mock micrococcal nuclease digestion was performed (lanes 7-10). + indicates a complementary oligonucleotide was included in the reaction; - indicates that the oligonucleotide was the same polarity as the template; RNAseH indicates E. coli RNAseH; 'No core' indicates the DHBV cores were omitted from the reaction. The sizes of the substrate (265 nt) and its cleavage products (152 nt and 93 nt) are marked.

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