Figure 3

Induction of Hsp104 and Hsp26 expression in SOD and TPS1 strains. Total cellular proteins from strains EG103 (control strain), EG110 (sod2), EG118 (sod1), EG133 (sod1/sod2), JT2700 (control strain) and JT9060 (tps1) were analyzed by Western blot with anti-Hsp104 and anti-Hsp26 protein antibody. Lane1- extracts from cultures at exponential growth on glucose; Lane2- extracts from cultures at exponential growth on glucose directly submitted to 5 mM H₂O₂; Lane3- extracts from cultures exposed to a mild heat treatment at 40°C/1 h and Lane4- extract from cultures pre-exposed to 40°/1 h and then submitted to a lethal treatment with 5 mM H₂O₂. Each lane contain the same quantity of protein (50 μg of protein).