Sulphur metabolism and cellulase gene expression are connected processes in the filamentous fungus Hypocrea jecorina (anamorph Trichoderma reesei)
© Gremel et al; licensee BioMed Central Ltd. 2008
Received: 17 June 2008
Accepted: 08 October 2008
Published: 08 October 2008
Sulphur compounds like cysteine, methionine and S-adenosylmethionine are essential for the viability of most cells. Thus many organisms have developed a complex regulatory circuit that governs the expression of enzymes involved in sulphur assimilation and metabolism. In the filamentous fungus Hypocrea jecorina (anamorph Trichoderma reesei) little is known about the participants in this circuit.
Analyses of proteins binding to the cellulase activating element (CAE) within the promotor of the cellobiohydrolase cbh2 gene led to the identification of a putative E3 ubiquitin ligase protein named LIMPET (LIM1), which is an orthologue of the sulphur regulators SCON-2 of Neurospora crassa and Met30p of Saccharomyces cerevisiae. Transcription of lim1 is specifically up-regulated upon sulphur limitation and responds to cellulase inducing conditions. In addition, light dependent stimulation/shut down of cellulase gene transcription by methionine in the presence of sulphate was observed. Further, lim1 transcriptionally reacts to a switch from constant darkness to constant light and is subject to regulation by the light regulatory protein ENVOY. Thus lim1, despite its function in sulphur metabolite repression, responds both to light as well as sulphur- and carbon source. Upon growth on cellulose, the uptake of sulphate is dependent on the light status and essential for growth in light. Unlike other fungi, growth of H. jecorina is not inhibited by selenate under low sulphur conditions, suggesting altered regulation of sulphur metabolism. Phylogenetic analysis of the five sulphate permeases found in the genome of H. jecorina revealed that the predominantly mycelial sulphate permease is lacking, thus supporting this hypothesis.
Our data indicate that the significance of the sulphate/methionine-related signal with respect to cellulase gene expression is dependent on the light status and reaches beyond detection of sulphur availability.
Gene transcription in filamentous fungi is influenced by a variety of external stimuli. Adaptation to nutrient conditions is one of them. Many sulphur compounds, especially cysteine, methionine and S-adenosylmethionine are essential for the viability of most cells . Thus, many organisms have developed a complex regulatory circuit that governs the expression of enzymes involved in sulphur assimilation and metabolism. Appropriate mechanisms have been discussed in the filamentous fungi Aspergillus nidulans and Neurospora crassa as well as the yeast Saccharomyces cerevisiae (reviewed by ). Neurospora crassa CYS3  and Saccharomyces cerevisiae Met4  were described as positive-acting regulators of an entire set of genes involved in sulphur metabolism. SCONB in Aspergillus nidulans [4, 5], SCON2 in Neurospora crassa [6, 7] and Met30 in Saccharomyces cerevisiae  on the other hand were identified as negative regulators. In recent years, the regulatory machinery of sulphur metabolism has been shown to be embedded in a more complex network, which also involves response to heavy metals  and is influenced by the supply of carbon and nitrogen in plants . The SCF-ubiquitin-ligase complex SCF(Met30) participates in cadmium-induced regulation of the transcription factor Met4  and thereby initiates a system wide cellular response. This SCF-ubiquitin ligase complex is also presumed to contribute to the detoxification of cadmium by increasing the glutathion levels needed for complexing this toxic heavy metal [12, 13]. A similar mechanism is likely to be at work for detoxification of arsenate  and selenate .
The filamentous fungus Hypocrea jecorina (anamorph Trichoderma reesei) is to date the most important industrial producer of cellulases [16–18]. Nevertheless, the genome of this fungus comprises a lower number of genes encoding these degradative enzymes than found in other filamentous fungi . The importance of cellulases as indispensable reagents in industries like the pulp and paper industry, detergent industry or clothing industry gives rise to attempts for elucidating the mechanisms lying behind cellulose signalling to cellulase gene expression in H. jecorina. Remarkable effort had thus been put into elucidation of the mechanisms involved in regulation of cellulase gene expression. Besides cellulose, also lactose and sophorose promote cellulase gene expression, while glycerol does neither promote nor prevent it. Formation of cellulases is regulated at the transcriptional level, and the expression of the different cellulase genes has been reported to be coordinate . However, little is known still about how the cellulose signal from outside the cell is transduced to initiate production of the appropriate hydrolytic enzymes. The first signal transduction protein to be identified in the respective signalling pathway was the light regulatory protein ENVOY , which also lead to the finding that cellulase gene expression is modulated by light. Subsequent analyses revealed that this protein influences several regulatory pathways in H. jecorina and that its function is not limited to light response .
Studies on the promotors of the cellulase genes provide a thorough basis to climb up the signal transduction cascade starting from the target: Using cell-free extracts from sophorose-induced and non-induced mycelia as well as various fragments of the promotor sequence of the H. jecorina cellobiohydrolase cbh2, the nucleotide sequence 5'-ATTGGGTAATA-3' was found to bind different protein complexes in electrophoretic mobility shift assays (EMSA) under the respective conditions . The mentioned sequence was named cbh2-activating element CAE. The presence of an intact copy of this motif was shown to be required for protein binding and wild-type levels of cbh2 transcript . While the HAP2/3/5 complex of H. jecorina was found to bind to the CCAAT-box of the cellulase activating element CAE within the cbh2 promoter  the factor(s) binding to the second part of the motif therein, which shows an even stronger regulatory effect (50% decrease in transcription efficiency upon mutation to 5' ATTGGGTTTTA 3') than the CCAAT-box, have not been described.
The transcriptional regulation of cellulase gene expression has been subject to extensive research [20, 25], and among the regulatory factors of this process, ACE2 , and XYR1 [27, 28] have been shown to bind to sequence motifs related yet not similar to CAE in the promotors of cbh1 (ACE2; 5' GGCTAATAA 3') or xyn1 (XYR1; 5' GGCTAA 3'). Despite the fact that their binding to CAE within the cbh2 promotor has not been proven yet, they are likely to contact this motif due to their involvement in regulation of cbh2 expression.
Our study was aimed at a more detailed understanding of cellulase gene regulation at the promotor level. Application of the Yeast One Hybrid System should identify the regulatory factor(s) binding to CAE. Surprisingly, we found a putative E3 ubiquitin ligase, the homologues of which are involved in regulation of sulphur metabolism, to bind to this regulatory sequence. This result led to the hypothesis that sulphur signaling/metabolism and regulation could be interconnected processes. In order to test this hypothesis we used different cultivation conditions appropriate to delineate the involvement of this E3 ubiquitin ligase in both processes and more importantly the role of the sulphur source with respect to cellulose utilization in H. jecorina in general. We show that the respective gene not only responds to cellulase inducing conditions and to light, but also – as expected – to a decrease in sulphur availability. Since methionine seems to have a specific function in regulation of cellulase gene expression under defined sulphur conditions, we tested its effect under conditions commonly used to analyse this process. Indeed, the strong light-dependent effect of increased methionine levels seen under defined sulphur conditions also occurs under these conditions i. e. in the presence of sulphate and peptone. Moreover, we detected a role of sulphate in cellulose utilization, but not glucose utilization in light. Subsequent screening of the H. jecorina genome for the presence of sulphate permeases revealed the lack of a homologue to the N. crassa predominantly mycelial sulphate permease CYS-14.
Microbial strains and culture conditions
H. jecorina (T. reesei) strain QM9414 (ATCC 26921), which is a second generation high cellulase producing mutant strain of wild-type QM6a and is commonly used for studies on this fungus representing wild-type conditions as well as the recombinant strain env1PAS-, which is lacking the PAS domain of the light regulatory protein ENVOY  were used throughout this study. H. jecorina was grown in submerged culture at 28°C in 1-liter Erlenmeyer flasks on a rotary shaker (200 rpm) in 200 ml of Mandels Andreotti minimal medium . 1% (w/v) of the carbon sources indicated with the respective experiments was used. 108 conidia/l (final concentration) were used as inoculum. The replacement technique described by Sternberg and Mandels  was applied for inducing cellulase formation in mycelia by 1.5 mM sophorose (final concentration), as described previously [23, 31].
The medium for cultivation under sulphur limitation conditions contained the respective chlorides instead of the sulphates used in conventional Mandels Andreotti medium and was prepared as follows: The modified trace element solution contained 0.243 g/l FeCl3·6H2O (0.90 mM), 0.050 g/l MnCl2·2H2O (0.31 mM), 0.033 g/l ZnCl2 (0.24 mM) and 0.100 g/l CoCl2·6H2O (0.42 mM) and the pH was adjusted to 2.0 with hydrochloric acid. The mineral salt solution contained 2.27 g/l NH4Cl (42 mM), 4.00 g/l KH2PO4 (29 mM), 0.50 g/l MgCl2·6H2O (2 mM), 0.80 g/l CaCl2·2H2O (5 mM) and 0.6 g/l urea (10 mM). A 0.1 M phosphate buffer was prepared using a solution with 35.6 g/l Na2HPO4·2H2O (0.2 M) and 0.2 M citric acid was added to adjust a pH of 5.0 (all chemicals by Merck, Germany). The culture medium was prepared combining 500 ml mineral salt solution, 480 ml phosphate buffer and 20 ml trace element solution.
As sulphur source, the following three concentrations of L-methionine (Sigma-Aldrich, St. Louis, USA) were used: 0.05 mM, 0.25 mM and 5 mM corresponding to limiting, low and high sulphur concentration, respectively .
E. coli JM109  was used for the propagation of vector molecules and DNA manipulations; E. coli ER1647 (Novagen, Madison, WI) was used for plating, titering and screening of the λ Blue Star gene library; and E. coli strain BM25.8 (Novagen), which is lysogenic for λ phages, was used for automatic subcloning. The latter two strains were grown at 37°C on LB medium supplemented with 0.2% (w/v) maltose and 10 mM MgSO4 for propagation of λ-phages. E. coli BL21 (DE3) (Stratagene, La Jolla, CA) was used for the expression of GST fusion proteins according to the recommendations of the manufacturers.
Nucleic acid isolation and blotting
H. jecorina DNA was isolated as described previously . Yeast and E. coli DNA was isolated as described by Sambrook et al. Total RNA was isolated by the guanidinium/phenol procedure . Northern blotting was performed as described previously [31, 35]. For transcript analysis of lim1 the insert of pOHS125 was excised and used for hybridization. Probes for analysis of transcription of env1, cbh1, cbh2 and 18S rRNA were prepared by PCR. Densitometric scanning was done using the BIO-RAD (Hercules, USA) GS-800 calibrated densitometer and the BIO-RAD Quantity One software for different exposures of the respective film.
Construction of Reporter Plasmids and Yeast strains for One-hybrid screening
Complementary oligonucleotides with three copies of the mutated CAE motif (oneH(F) 5' AATTCTCTTTAAAGGGTAATATACAGCCATCTTTAAAGGGTAATATACAGCCATCTTTAAAGGGTAATATACAGCCAT 3') containing the functional (GG)GTAATA-sequence and a non-functional CCAAT box  corresponding to positions – 253 to – 220 of the cbh2 promoter were cloned into plasmids pHISi and pLacZi. For the construction of a negative control plasmid also the (GG)GTAATA sequence was mutated to (GG)GTTTTA in the oligonucleotide to abolish binding of the respective proteins . The resulting plasmids were designated pHISi-CAE and pLacZi-CAE or pHISi-mCAE, respectively.
A reporter strain HIS-LACZ-CAE for the transformation of the cDNA library was constructed by integration of pHISi-CAE and pLacZi-CAE into yeast strain YM4271. The negative control strain HIS-mCAE was obtained by integration of pHISi-mCAE into yeast strain YM4271. Plasmids and yeast strain YM4271 were included in the Matchmaker One Hybrid system kit (CLONTECH, Palo Alto, USA).
Construction of an Activation Domain tagged cDNA-Library from H. jecorinaby Directional cloning
For preparation of cDNA, mycelia of the wild-type strain QM9414, grown under cellulase inducing conditions (sophorose) were used. mRNA was isolated from total RNA using the PolyATtract mRNA Isolation Kit (Promega, Madison, USA), and a cDNA library was constructed using the HybriZAP™ Two-Hybrid cDNA Gigapack® Cloning Kit (Stratagene Ltd., Cambridge, UK) according to the manufacturers instructions. After packaging by the Gigapack III Gold packaging extract (Stratagene Ltd, Cambridge, UK) and excision, the cDNA library containing 106 independent clones within the vector pAD-GAL4-2.1 in frame to the GAL4 activation domain was used in the One Hybrid System.
Yeast One hybrid Screening of a H. jecorinacDNA Library
The yeast reporter strain HISLACZ-CAE was transformed using the lithium acetate/ssDNA/PEG method . The resulting 3 × 105 yeast transformants were plated on his-/leu- selective media containing 50 mM 3-aminotriazole (3-AT). The concentration of 50 mM 3-AT was previously determined to be sufficient to suppress leaky expression of the marker gene. Positive transformants were additionally tested for β-galactosidase activity by a filter lift assay according to the manufacturer's instructions (Matchmaker One-Hybrid system Kit, CLONTECH). Plasmids from putative positive clones were isolated after homogenization with glass beads, amplified in E. coli JM109 , and retransformed into yeast strain HISLACZ-CAE and tested for growth in the presence of 50 mM 3-AT. This step was followed by transformation of plasmids conferring histidine prototrophy into the negative control strain HIS-mCAE. The rationale was that the mutation abolishes protein binding in the yeast cell, and positive transformants should therefore be unable to grow on selective media.
Cloning of chromosomal DNA of H. jecorina
To isolate the corresponding genomic clones, the cDNA fragment obtained from the experiment was used as probe to screen a genomic λ Blue Star (Novagen, Madison, Wis.) library of H. jecorina QM9414. Positive clones were excised from the phages by automatic subcloning following the instructions of the manufacturer. The sequence was crosschecked with the Trichoderma reesei Genome database v2.0 .
First strand synthesis was performed using the Reverse Transcription System (Promega, Madison, USA) according to the manufacturers protocol at 42°C for 1 hour with primer RACE-N (5' GCGTAATACGACTCACTATAGGGCGAATTGGGTTTTTTTTTTTTTTTTT(AGC) 3'). The reaction mixture was cleaned up using the QIAquick PCR Cleanup Kit (QIAGEN). PCR and Nested PCR were performed according to standard protocols using a gene specific primer and primer RACE-N22 (5' GCGTAATACGACTCACTAT AGG 3').
Determination of biomass formation from cellulose grown cultures
After 96 hours of growth on microcrystalline cellulose either in light or in darkness, mycelia were harvested by centrifugation and 5 ml 0.1 N NaOH were added to the pellet. The sample was sonicated for 3 minutes and incubated for 3 hours at room temperature. Following centrifugation, protein content of the supernatant representing biomass content of the sample was determined using the BIO-RAD Protein assay (BIO-RAD, Hercules, US). Samples were analyzed in triplicates from independent cultivations.
Purification of glutathione S-transferase fusion proteins
The cDNA-insert encoding aa457 – aa636 of LIM1 as isolated with the One Hybrid system was cloned into vector pGEX4T-1 (Stratagene). E. coli BL21 (Stratagene) was transformed with the expression construct. Expression and purification by glutathione sepharose 4B (Amersham Pharmacia Biotech, Uppsala, Sweden) affinity chromatography was essentially performed as described previously . Fusion proteins were eluted using 50 mM Tris-HCl, pH 8.0, 10 mM glutathione and 2 mM dithiothreitol. All protein preparations were stored at -80°C in the presence of 20% (w/v) glycerol.
Electrophoretic mobility shift assay
Electrophoretic mobility shift assays were performed as described by Stangl et al. The oligonucleotides were end-labelled with (α-32P)-dCTP, using Sequenase version 2.0 (Amersham, Little Chalfont, UK). After purification by nondenaturing polyacrylamide gel electrophoresis binding was achieved by incubating 5 μg of protein with 5 ng of labelled fragment at room temperature for 10 min. GST-elution buffer (see above) containing 20% glycerol and lacking protein was used as negative control (sample referred to as free probe).
Amino acid sequences were aligned with Clustal X 1.81  and then visually adjusted. Phylogenetic analyses were performed in MEGA 2.1 using the Minimum Evolution (ME) approach. Reliability of nodes was estimated by ME bootstrap percentages (BPME)  obtained after 500 pseudo-replications.
Identification of a protein that binds to CAE by the Yeast One Hybrid System
We applied the yeast one hybrid system to identify proteins binding to CAE , as this approach allows to screen for DNA-protein interaction in vivo and thus bypasses common drawbacks of in vitro experiments such as altered binding characteristics caused by the experimental conditions or problems with the renaturation of the proteins in vitro [44–46].
The protein encoded on pOHS125 also binds to CAE in vitro
Since the protein encoded on pOHS125 was cloned by its ability to bind to the CAE motif in vivo in the One Hybrid Assay, we wanted to confirm this result in vitro by Electrophoretic mobility shift assay. To this end, the cDNA-insert from pOHS125 (encoding aa457 – aa636, which was functional in yeast) was cloned in frame into pGEX4T-1, expressed in E. coli as GST-fusion protein, purified and used in gel retardation assays. The EMSA analysis indicated that the fusion protein actually binds to the target sequence on the oligonucleotide oneH-CAE, albeit the interaction was very weak (Figure 1C).
LIMPET encodes a WD-Repeat/F-box protein
lim1is regulated in dependence of sulphur availability
LIMPET responds to cellulase-inducing conditions
Sulphate is important for normal growth of H. jecorinain light on cellulose
Sulphate uptake is regulated by light in H. jecorina
The finding that light-dependent biomass formation of H. jecorina on cellulose is clearly altered upon growth in medium lacking sulphate led to the hypothesis that uptake of inorganic sulphur is important for growth on cellulosic substrates in light. This hypothesis was tested in the following. Sensitivity versus toxic sulphur analogons such as selenate and chromate has been reported under conditions of low organic sulphur in wild-type strains of Aspergillus nidulans as well as Neurospora crassa [5, 51]. This phenomenon is due to derepression of the sulphate uptake, when the organic sulphur source becomes limiting . Because of the chemical analogy of selenate to sulphate, both are taken up into the cell by the same permeases [53, 54] and also metabolism of selenate follows the same route as for sulphate . The toxicity of selenate is consequently due to toxic by-products generated during reduction of selenate, which cause oxidative damage of DNA and addition of sulphate alleviates the toxicity of selenate [54, 56]. Hence growth defects upon cultivation in the presence of selenate are indicative of selenate uptake into the cell. Considering the co-transport of sulphate and selenate such a growth defect also represents initiated sulphate uptake under the respective conditions.
Using plate experiments the presence of light-dependent regulation of sulphate uptake should be verified. Therefore plates containing sulphate free Mandels Andreotti medium with 1% (w/v) of (soluble) carboxymethylcellulose, 0.05 mM of methionine and either 1 mM of Na2SeO4 or 2 mM of Na2SO4 were inoculated with agar plugs taken from fully sporulated plates of strain QM9414. Afterwards they were exposed to light or kept in constant darkness under otherwise equal conditions at 28°C for four days.
In the presence of 0.05 mM (limiting) methionine Na2SeO4 was supposed to inhibit the growth of the fungus due to sulphur metabolite derepression . In fact H. jecorina was not inhibited as severely as expected in growth by the presence of selenate and limiting concentrations of methionine. The observed colony diameter after four days of incubation of strain QM9414 was only slightly decreased on selenate compared to sulphate in constant darkness (2.7 ± 0.1 cm on selenate or 3.0 ± 0.1 cm on sulphate) i. e. by 10%, but was clearly reduced in light (2.5 ± 0.3 cm on selenate or 3.8 ± 0.2 cm on sulphate) i. e. by 35%. No such substantial light-dependent difference in selenate sensitivity was observed when the same experiment was done using glucose as carbon source under otherwise equal conditions (5.2 ± 0.4 cm on selenate or 6.2 ± 0.1 cm on sulphate in darkness (16%) and 4.9 ± 0.3 cm on selenate or 5.5 ± 0.4 cm on sulphate in light (12%)). Interestingly, increased concentrations of selenate caused only a minor decrease in growth up to 3 mM and no further decrease even with 10 mM selenate (data not shown), which completely inhibits growth of Aspergillus and Neurospora. The conclusion that sulphate uptake is increased during growth on carboxymethylcellulose in light (and therefore inhibited by the presence of selenate) is in perfect agreement with the data of biomass formation on microcrystalline cellulose as described above.
H. jecorinalacks a homologue of the predominantly mycelial sulphate permease
Sequences used for phylogenetic analysis of sulphate permeases
Neurospora crassa sulfate permease II cys-14
Saccharomyces cerevisiae Sul2p: high affinity sulfate permease
Penicillium chrysogenum sulphate permease SutB
Penicillium chrysogenum sulphate permease SutA
Cryptococcus neoformans sulfate transporter, putative
Aspergillus nidulans sulphate permease SUL1
Schizosaccharomyces pombe sulphate permease SPBC3H7.02
Saccharomyces cerevisiae sulfate permease.
Schizosaccharomyces pombe sulphate permease SPAC869.05c
Kluyveromyces lactis unnamed protein product
Neurospora crassa sulphate permease NCU04433.1
Neurospora crassa hypothetical protein NCU03235.1
Cryptococcus neoformans hypothetical protein CNBC0120
Cryptococcus neoformans hypothetical protein CNBC0120
Cryptococcus neoformans hypothetical protein CNBA0650
Magnaporthe grisea hypothetical protein MGG_ch7g1014
Chaetomium globosum hypothetical protein CHGG_03941
Chaetomium globosum hypothetical protein CHGG_05246
Chaetomium globosum hypothetical protein CHGG_00491
Chaetomium globosum hypothetical protein CHGG_01877
Phaeosphaeria nodorum hypothetical protein SNOG_14281
Phaeosphaeria nodorum hypothetical protein SNOG_13322
Phaeosphaeria nodorum hypothetical protein SNOG_08820
Phaeosphaeria nodorum hypothetical protein SNOG_08888
Phaeophaeria nodorum hypothetical protein SNOG_04510
Botryotinia fuckeliana hypothetical protein BC1G_12094
Saccharomyces cerevisiae sulphate permease Sul1p
Saccharomyces cerevisiae putative protein of unknown function
Saccharomyces cerevisiae sulphate permease Sul2p
Schizosaccharomyces pombe hypothetical protein SPCC320.05
Neurospora crassa sulphate permease 2 CYS-14
Saccharomyces cerevisiae Putative sulfate transporter YPR003C
H. jecorina sulphate permease
H. jecorina sulphate permease
H. jecorina sulphate permease
H. jecorina sulphate permease
H. jecorina sulphate permease
Magnaporthe grisea hypothetical protein MG05144.4
Magnaporthe grisea hypothetical protein MG04126.4
Magnaporthe grisea hypothetical protein MGG_04433
Magnaporthe grisea hypothetical protein MG04640.4
Magnaporthe grisea hypothetical protein MG09838.4
Magnaporthe grisea hypothetical protein MG10251.4
Gibberella zeae hypothetical protein FG01006.1
Gibberella zeae hypothetical protein FG01066.1
Gibberella zeae hypothetical protein FG02163.1
Gibberella zeae hypothetical protein FG04242.1
Gibberella zeae hypothetical protein FG11115.1
Gibberella zeae hypothetical protein FG11293.1
Aspergillus nidulans hypothetical protein AN3157.2
Aspergillus nidulans hypothetical protein AN3665.2
Ustilago maydis hypothetical protein UM05933.1
Neurospora crassa hypothetical protein NCU09642.1
Expression of lim1and cellulase genes is dependent on the availability of the sulphur source
Due to the obvious indirect correlation of transcription of lim1 and cbh1 in darkness, we were interested whether this correlation would also appear under commonly used conditions i. e. in conventional Mandels Andreotti medium, which contains 10 g/l peptone (Merck, #1.07213; corresponding to 0.25 mM methionine) and approx. 12 mM sulphate. Upon growth on conventional Mandels Andreotti medium, lim1 was detected not only in darkness, but also during growth in light. The complementary regulation of lim1 and cbh1 as seen under controlled sulphur conditions and upon sophorose-induction was not obvious in conventional Mandels Andreotti medium (Figure 7B). Since no inorganic sulphate is available in the sulphate free Mandels Andreotti minimal medium, it seems likely that lim1 responds to the kind of sulphur source available or even specifically to the presence of methionine if sulphate is lacking both by an altered transcript level in general as well as by an altered response to light.
lim1shows a transcriptional response to light and is regulated by ENVOY
Cellulase gene transcription is influenced contrarily in light and darkness by methionine
Sulphur uptake and metabolism are crucial processes for all organisms because of the manifold roles of the two sulphur containing amino acids cysteine and methionine not only in protein biosynthesis but also in redox potential homeostasis and methylation processes. Organisms have therefore developed sophisticated regulatory mechanisms to ensure controlled sulphur availability. As for fungi, the best analyzed machineries for this purpose are those of N. crassa, A. nidulans and particularly S. cerevisiae. In H. jecorina sulphur metabolism has not been studied so far.
The analysis of the influence of the sulphur source on regulation of cellulase gene expression was initiated by the unexpected finding, that LIMPET binds to a cis-regulatory motif of the cbh2 promotor. LIMPET is the first WD-repeat/F-box protein to be identified and characterized in H. jecorina. Concluding from the functions of its orthologues in other fungi, LIMPET could be a regulatory protein that activates transcription by phosphorylation-dependent degradation of a factor inhibiting expression of cbh2. Such a function would be in agreement with data from Zeilinger et al., , who showed that the complexes formed on CAE (the cbh2 activating element) are smaller under inducing conditions (sophorose), albeit the respective kinase and this probably repressing factor remain to be identified. Given the fact that E3 ubiquitin ligases themselves are frequently targets of degradation in an SCF-dependent manner [61, 62], LIMPET could also itself represent this repressor. While deletion of the lim1-orthologues scon2 (Neurospora crassa) or sconb (Aspergillus nidulans) was successful, we were not able to obtain viable lim1-deletion mutants, what may be due to the obviously altered sulphur regulatory pathway. Interestingly, the S. cerevisiae orthologue of lim1, met30 is also an essential gene . Nevertheless, we can therefore not be sure, whether LIMPET is indeed the transducer of the sulphur signal to the cbh2-promotor and thus a direct regulator of cellulase gene expression, although a connection between sulphur- and carbon-signaling became clearly obvious.
A hypothesis how LIMPET could impact cellulase gene expression can be deduced from the studies on regulation of sulphur metabolism in N. crassa: The regulatory cycle of SCON-2 and CYS-3 has been analyzed in detail in N. crassa and involves both regulation of transcription and activation of the respective proteins. During sulphur limitation, SCON-2 is present in high amounts, most probably in an inactive state. If the nutritional situation changes and an abundant sulphur source is encountered, this pool of SCON-2 can be rapidly activated and prevent the function of CYS-3, and thus shut down the sulphur circuit. This finally results in the termination of sulphur uptake. On the other hand expression of the scon-2 gene, the promotor of which contains several CYS-3 binding sites, is dependent on functional CYS-3 [6, 63]. Thus, SCON-2 inhibits cys3 transcription in a negative feedback loop . CYS-3 activates the uptake of sulphate and several other mechanisms involved in sulphur metabolism . Our data show that lim1 is not transcribed in light under conditions lacking an inorganic sulphur source. A function of LIMPET putatively analogous to SCON2 would thus lead to stimulated uptake of sulphate in light, which is not available under the condition tested, obviously resulting in considerably decreased growth. Also the decreasing effect of selenate upon growth on carboxymethylcellulose in light is in agreement with these data. Hence we conclude that sulphate is important for normal growth in light on cellulose and that methionine, being commonly considered an organic sulphur source, cannot compensate for this requirement. However, we want to note that despite the presence of a CYS-3 homologue, no binding sites similar to those identified in N. crassa were detected in the promotor of lim1. Hence a negative feedback loop may not be operative in H. jecorina.
Although the finding of LIMPET as a close homologue of N. crassa SCON-2 or A. nidulans SCONB, respectively, suggests similarities in the sulphur regulatory systems of Hypocrea, Neurospora and Aspergillus, the mechanism of sulphate uptake in Hypocrea jecorina QM9414 appears to be altered: First, phylogenetic analysis of its predicted sulphate permeases indicates, that the predominantly mycelial sulphate permease (the CYS-14 orthologue) is missing. Transcription of the remaining, predominantly conidial sulphate permease (the CYS-13 orthologue) could not be detected under any conditions tested (M. Schmoll, data not shown). In this respect it is particularly interesting, that lack of the two sulphate permeases CYS-13 and CYS-14 leads to the inability to use sulphate as sulphur source and to selenate resistence  in N. crassa, although 4 sulphate permease genes have been detected in this fungus . For both N. crassa and A. nidulans [5, 51] sensitivity versus toxic sulphur analogues in presence of low concentrations (0.25 mM) of methionine was described, which is not found for H. jecorina. In S. cerevisiae, regulation of the synthesis of both sulphate permeases is under the control of exogenous methionine or S-adenosylmethionine. Their synthesis is coordinated with the synthesis of the other methionine biosynthetic enzymes , what would imply continued uptake of selenate and thus lethality under low-methionine conditions. Judging from this data, the H. jecorina sulphur assimilation system appears to exhibit high affinity versus methionine, enabling the fungus to survive even in the presence of very low concentrations of it. Since this fungus can also utilize sulphate as sole sulphur source, it is likely that H. jecorina is – obviously unlike A. nidulans or N. crassa – able to differentiate between these sulphur sources (which is also reflected by the transcription pattern of lim1 on media with or without sulphate) and terminate one uptake mechanism in favour of the other if required. In case of the presence of selenate such a flexible mechanism could prevent the fatal effect of this sulphate analogon, although therefore a specific detection of the toxin would be required. Alternatively an as yet unidentifed detoxification mechanism, which would explain this effect could be at work. In this respect it is interesting, that for several yeast species growing on decaying fruits comparable mechanisms have been detected and suggested to have evolved due to the considerable amounts of selenate present in certain plants. In order to avoid the harmful consequences of sulphate and thus selenate uptake they can preferentially utilize organic sulphur, in many cases obtained by attacking other yeasts [65, 66]. Data on the role of the S. cerevisiae homologue of LIMPET, Met30p clearly show a role of the sulphur regulatory machinery in heavy metal response through regulation of the transcription factor Met4p . The main target of this circuit in this case is glutathione synthesis, which is needed to complex and subsequently detoxify the heavy metal. A mechanism as described above would be in concordance with the symbiotic interaction of Trichoderma spp. with plants , their natural substrate being decaying plant material and their efficient biocontrol activity . The fact that both the presence of selenate/sulphate or methionine, respectively, has different consequences in light and darkness further indicates that these environmental cues must have a more significant relevance than just signalling the availability of sulphur. In accordance with this hypothesis we found that H. jecorina adjusts cellulase gene transcription in dependence of the sulphur source available and the light status. Considering the mechanisms discussed above the connecting environmental cues might be the presence of plant material (cellulose) and possibly the encounter of a competitor, which is more likely on the surface of the substrate (light).
Consequently, an involvement of LIMPET in modulation of cellulase gene expression in response to a varying supply of sulphur seems to be beneficial for H. jecorina. Interestingly, the respective signal leads to opposite effects in light and darkness in the presence of sulphate. Therefore we hypothesize that the significance of the presence of methionine reaches beyond merely an indication of the sulphur source. Since the addition of methionine did not stimulate growth on cellulose in light in the absence of sulphate, we assume that its effect is also not simply related to the provision of a potentially growth-limiting amino acid. The response to methionine may be – at least in part – connected to the intracellular cAMP-levels which has been shown to stimulate cellulase formation in H. jecorina  and Cryphonectria parasitica . While the enhanced transcription of cbh1 upon addition of methionine in darkness is well in concordance with this hypothesis, the abolished cbh1 transcription under the same conditions in light rather indicates a more sophisticated regulation. A recent study by Xue and coworkers  provides intriguing hints which might shed a new light on the interacting signal transduction pathways studied in this work. They show that the G-protein coupled receptor GPR4 of Cryptococcus neoformans, which has an orthologue in H. jecorina (tre72004), interacts with the G-protein α subunit GPA1, the orthologue of H. jecorina GNA3 and that methionine induces cAMP accumulation in this fungus. This study on the one hand shows that this G-protein coupled receptor does not respond to glucose as expected from its structural similarity to the S. cerevisiae glucose sensor GPR1, but instead seems to sense methionine – this amino acid being the only clearly confirmed ligand of GPR4. On the other hand, its interacting G-protein α subunit GPA1 is involved in glucose sensing. Also, methionine was found to induce cAMP accumulation in C. neoformans.
We want to thank Christian P. Kubicek for critically reading the manuscript and S. Zeilinger and R. Mach for assistance in designing the One Hybrid experiment. This work has been supported by grants from the Austrian Science Foundation (FWF-P17325) to Christian P. Kubicek. MS is recipient of an APART fellowship of the Austrian Academy of Sciences at the Institute of Chemical Engineering, TU Vienna. The T. reesei genome sequencing project was funded by the Department of Energy, USA.
- Marzluf GA: Molecular genetics of sulfur assimilation in filamentous fungi and yeast. Annu Rev Microbiol. 1997, 51: 73-96.View ArticlePubMedGoogle Scholar
- Paietta JV, Akins RA, Lambowitz AM, Marzluf GA: Molecular cloning and characterization of the cys-3 regulatory gene of Neurospora crassa. Mol Cell Biol. 1987, 7 (7): 2506-2511.PubMed CentralView ArticlePubMedGoogle Scholar
- Thomas D, Jacquemin I, Surdin-Kerjan Y: MET4, a leucine zipper protein, and centromere-binding factor 1 are both required for transcriptional activation of sulfur metabolism in Saccharomyces cerevisiae. Mol Cell Biol. 1992, 12 (4): 1719-1727.PubMed CentralView ArticlePubMedGoogle Scholar
- Natorff R, Balinska M, Paszewski A: At least four regulatory genes control sulphur metabolite repression in Aspergillus nidulans. Mol Gen Genet. 1993, 238 (1–2): 185-192.PubMedGoogle Scholar
- Natorff R, Piotrowska M, Paszewski A: The Aspergillus nidulans sulphur regulatory gene sconB encodes a protein with WD40 repeats and an F-box. Mol Gen Genet. 1998, 257 (3): 255-263.View ArticlePubMedGoogle Scholar
- Kumar A, Paietta JV: The sulfur controller-2 negative regulatory gene of Neurospora crassa encodes a protein with beta-transducin repeats. Proc Natl Acad Sci USA. 1995, 92 (8): 3343-3347.PubMed CentralView ArticlePubMedGoogle Scholar
- Kumar A, Paietta JV: An additional role for the F-box motif: gene regulation within the Neurospora crassa sulfur control network. Proc Natl Acad Sci USA. 1998, 95 (5): 2417-2422.PubMed CentralView ArticlePubMedGoogle Scholar
- Thomas D, Kuras L, Barbey R, Cherest H, Blaiseau PL, Surdin-Kerjan Y: Met30p, a yeast transcriptional inhibitor that responds to S-adenosylmethionine, is an essential protein with WD40 repeats. Mol Cell Biol. 1995, 15 (12): 6526-6534.PubMed CentralView ArticlePubMedGoogle Scholar
- Yen JL, Su NY, Kaiser P: The yeast ubiquitin ligase SCFMet30 regulates heavy metal response. Mol Biol Cell. 2005, 16 (4): 1872-1882.PubMed CentralView ArticlePubMedGoogle Scholar
- Maruyama-Nakashita A, Nakamura Y, Yamaya T, Takahashi H: Regulation of high-affinity sulphate transporters in plants: towards systematic analysis of sulphur signalling and regulation. J Exp Bot. 2004, 55 (404): 1843-1849.View ArticlePubMedGoogle Scholar
- Baudouin-Cornu P, Labarre J: Regulation of the cadmium stress response through SCF-like ubiquitin ligases: comparison between Saccharomyces cerevisiae, Schizosaccharomyces pombe and mammalian cells. Biochimie. 2006, 88 (11): 1673-1685.View ArticlePubMedGoogle Scholar
- Barbey R, Baudouin-Cornu P, Lee TA, Rouillon A, Zarzov P, Tyers M, Thomas D: Inducible dissociation of SCF(Met30) ubiquitin ligase mediates a rapid transcriptional response to cadmium. Embo J. 2005, 24 (3): 521-532.PubMed CentralView ArticlePubMedGoogle Scholar
- Fauchon M, Lagniel G, Aude JC, Lombardia L, Soularue P, Petat C, Marguerie G, Sentenac A, Werner M, Labarre J: Sulfur sparing in the yeast proteome in response to sulfur demand. Mol Cell. 2002, 9 (4): 713-723.View ArticlePubMedGoogle Scholar
- Haugen AC, Kelley R, Collins JB, Tucker CJ, Deng C, Afshari CA, Brown JM, Ideker T, van Houten B: Integrating phenotypic and expression profiles to map arsenic-response networks. Genome Biol. 2004, 5 (12): R95-PubMed CentralView ArticlePubMedGoogle Scholar
- Pinson B, Sagot I, Daignan-Fornier B: Identification of genes affecting selenite toxicity and resistance in Saccharomyces cerevisiae. Mol Microbiol. 2000, 36 (3): 679-687.View ArticlePubMedGoogle Scholar
- Buchert J, Oksanen T, Pere J, Siika-Aho M, Suurnäkki A, Viikari L: Applications of Trichoderma reesei enzymes in the pulp and paper industry. Trichoderma & Gliocladium. Edited by: Harman GE, CP K. 1998, London: Taylor & Francis, 2: 343-363.Google Scholar
- Galante Y, De Conti A, Monteverdi R: Application of Trichoderma enzymes in the textile industry. Trichoderma & Gliocladium. Edited by: Harman GE, KC. 1998, London: Taylor & Francis, 311-325.Google Scholar
- Galante Y, De Conti A, Monteverdi R: Application of Trichoderma enzymes in the food and feed industries. Trichoderma and Gliocladium. Edited by: Harman GE, Kubicek CP. 1998, London: Taylor & Francis, 327-342.Google Scholar
- Martinez D, Berka RM, Henrissat B, Saloheimo M, Arvas M, Baker SE, Chapman J, Chertkov O, Coutinho PM, Cullen D, et al: Genome sequencing and analysis of the biomass-degrading fungus Trichoderma reesei (syn. Hypocrea jecorina. Nat Biotechnol. 2008, 26 (5): 553-560.View ArticlePubMedGoogle Scholar
- Schmoll M, Kubicek CP: Regulation of Trichoderma cellulase formation: lessons in molecular biology from an industrial fungus. A review. Acta Microbiol Immunol Hung. 2003, 50 (2–3): 125-145.View ArticlePubMedGoogle Scholar
- Schmoll M, Franchi L, Kubicek CP: Envoy, a PAS/LOV domain protein of Hypocrea jecorina (Anamorph Trichoderma reesei), modulates cellulase gene transcription in response to light. Eukaryot Cell. 2005, 4 (12): 1998-2007.PubMed CentralView ArticlePubMedGoogle Scholar
- Schuster A, Kubicek CP, Friedl MA, Druzhinina IS, Schmoll M: Impact of light on Hypocrea jecorina and the multiple cellular roles of ENVOY in this process. BMC Genomics. 2007, 8 (1): 449-PubMed CentralView ArticlePubMedGoogle Scholar
- Zeilinger S, Mach RL, Kubicek CP: Two adjacent protein binding motifs in the cbh2 (cellobiohydrolase II-encoding) promoter of the fungus Hypocrea jecorina (Trichoderma reesei) cooperate in the induction by cellulose. J Biol Chem. 1998, 273 (51): 34463-34471.View ArticlePubMedGoogle Scholar
- Zeilinger S, Ebner A, Marosits T, Mach R, Kubicek CP: The Hypocrea jecorina HAP 2/3/5 protein complex binds to the inverted CCAAT-box (ATTGG) within the cbh2 (cellobiohydrolase II-gene) activating element. Mol Genet Genomics. 2001, 266 (1): 56-63.View ArticlePubMedGoogle Scholar
- Aro N, Pakula T, Penttila M: Transcriptional regulation of plant cell wall degradation by filamentous fungi. FEMS Microbiol Rev. 2005, 29 (4): 719-739.View ArticlePubMedGoogle Scholar
- Aro N, Saloheimo A, Ilmen M, Penttila M: ACEII, a novel transcriptional activator involved in regulation of cellulase and xylanase genes of Trichoderma reesei. J Biol Chem. 2001, 276 (26): 24309-24314.View ArticlePubMedGoogle Scholar
- Rauscher R, Wurleitner E, Wacenovsky C, Aro N, Stricker AR, Zeilinger S, Kubicek CP, Penttila M, Mach RL: Transcriptional regulation of xyn1, encoding xylanase I, in Hypocrea jecorina. Eukaryot Cell. 2006, 5 (3): 447-456.PubMed CentralView ArticlePubMedGoogle Scholar
- Stricker AR, Grosstessner-Hain K, Wurleitner E, Mach RL: Xyr1 (Xylanase Regulator 1) Regulates both the Hydrolytic Enzyme System and D-Xylose Metabolism in Hypocrea jecorina. Eukaryot Cell. 2006, 5 (12): 2128-2137.PubMed CentralView ArticlePubMedGoogle Scholar
- Mandels M, Andreotti R: Problems and challenges in the cellulose to cellulase fermentation. Proc Biochem. 1978, 13: 6-13.Google Scholar
- Sternberg D, Mandels GR: Induction of cellulolytic enzymes in Trichoderma reesei by sophorose. J Bacteriol. 1979, 139 (3): 761-769.PubMed CentralPubMedGoogle Scholar
- Schmoll M, Zeilinger S, Mach RL, Kubicek CP: Cloning of genes expressed early during cellulase induction in Hypocrea jecorina by a rapid subtraction hybridization approach. Fungal Genet Biol. 2004, 41 (9): 877-887.View ArticlePubMedGoogle Scholar
- Liu J, Wilson TE, Milbrandt J, Johnston M: Identifying DNA-binding sites and analyzing DNA-binding domains using a yeast selection system. Methods: A Companion to Methods in Enzymology. 1993, 5: 125-137.View ArticleGoogle Scholar
- Wilson TE, Fahrner TJ, Johnston M, Milbrandt J: Identification of the DNA binding site for NGFI-B by genetic selection in yeast. Science. 1991, 252: 1296-1300.View ArticlePubMedGoogle Scholar
- Yanisch-Perron C, Vieira J, Messing J: Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985, 33 (1): 103-119.View ArticlePubMedGoogle Scholar
- Sambrook J, Fritsch EF, Maniatis T: Molecular cloning: a Laboratory Manual. 1989, NY: Cold Spring Harbour Laboratory Press, 2Google Scholar
- Chomczynski P, Sacchi N: Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem. 1987, 162 (1): 156-159.View ArticlePubMedGoogle Scholar
- Gietz RD, Woods RA: Transformation of yeast by the lithium acetate/single stranded carrier DNA/PEG method. Methods in Enzymology. 2002, 350: 87-96.View ArticlePubMedGoogle Scholar
- Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K: Current Protocols in Molecular Biology. 1993, New York: Greene Publishing Associates and Wiley-InterscienceGoogle Scholar
- Trichoderma reesei genome database ver2.0. [http://genome.jgi-psf.org/Trire2/Trire2.home.html]
- Smith DB, Johnson KS: Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene. 1988, 67 (1): 31-40.View ArticlePubMedGoogle Scholar
- Stangl H, Gruber F, Kubicek CP: Characterization of the Trichoderma reesei cbh2 promoter. Curr Genet. 1993, 23 (2): 115-122.View ArticlePubMedGoogle Scholar
- Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG: The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res. 1997, 25 (24): 4876-4882.PubMed CentralView ArticlePubMedGoogle Scholar
- Felsenstein J: Confidence limits on phylogenies: An approach sing the bootstrap. Evolution. 1985, 39: 783-791.View ArticleGoogle Scholar
- Chong JA, Mandel G: Isolation of DNA-bindng proteins using One Hybrid Genetic Screens. The Yeast Two Hybrid System. Edited by: Bartel PLFS. 1997, New York: Oxford University Press, 289-297.Google Scholar
- Kadonga JT, Tijan KR: Affinity purification of sequence-specific DNA binding proteins. Proc Natl Acad Sci. 1986, 83: 5889-5893.View ArticleGoogle Scholar
- Singh H, Lebowitz JH, Baldwin JAS, Sharp PA: Molecular cloning of an enhancer binding-protein: isolation by screening of an expression library with a recognition site DNA. Cell. 1988, 52: 415-423.View ArticlePubMedGoogle Scholar
- Schouten J, de Kam RJ, Fetter K, Hoge JH: Overexpression of Arabidopsis thaliana SKP1 homologues in yeast inactivates the Mig1 repressor by destabilising the F-box protein Grr1. Mol Gen Genet. 2000, 263 (2): 309-319.PubMedGoogle Scholar
- Psort II protein analysis server. [http://psort.ims.u-tokyo.ac.jp/]
- Reinhardt A, Hubbard T: Using neural networks for prediction of the subcellular location of proteins. Nucleic Acids Res. 1998, 26 (9): 2230-2236.PubMed CentralView ArticlePubMedGoogle Scholar
- Bai C, Sen P, Hofmann K, Ma L, Goebl M, Harper JW, Elledge SJ: SKP1 connects cell cycle regulators to the ubiquitin proteolysis machinery through a novel motif, the F-box. Cell. 1996, 86 (2): 263-274.View ArticlePubMedGoogle Scholar
- Paietta JV: Molecular cloning and analysis of the scon-2 negative regulatory gene of Neurospora crassa. Mol Cell Biol. 1990, 10 (10): 5207-5214.PubMed CentralView ArticlePubMedGoogle Scholar
- NCBI Blast server. [http://www.ncbi.nlm.nih.gov/BLAST/]
- Marzluf GA: Genetic and metabolic controls for sulfate metabolism in Neurospora crassa: isolation and study of chromate-resistant and sulfate transport-negative mutants. J Bacteriol. 1970, 102 (3): 716-721.PubMed CentralPubMedGoogle Scholar
- Raspor P, Fujs S, Banszky L, Maraz A, Batic M: The involvement of ATP sulfurylase in Se(VI) and Cr(VI) reduction processes in the fission yeast Schizosaccharomyces pombe. Appl Microbiol Biotechnol. 2003, 63 (1): 89-95.View ArticlePubMedGoogle Scholar
- Banszky L, Simonics T, Maraz A: Sulphate metabolism of selenate-resistant Schizosaccharomyces pombe mutants. J Gen Appl Microbiol. 2003, 49 (5): 271-278.View ArticlePubMedGoogle Scholar
- Letavayova L, Vlasakova D, Spallholz JE, Brozmanova J, Chovanec M: Toxicity and mutagenicity of selenium compounds in Saccharomyces cerevisiae. Mutat Res. 2008, 638 (1–2): 1-10.View ArticlePubMedGoogle Scholar
- Borkovich KA, Alex LA, Yarden O, Freitag M, Turner GE, Read ND, Seiler S, Bell-Pedersen D, Paietta J, Plesofsky N, et al: Lessons from the genome sequence of Neurospora crassa: tracing the path from genomic blueprint to multicellular organism. Microbiol Mol Biol Rev. 2004, 68 (1): 1-108.PubMed CentralView ArticlePubMedGoogle Scholar
- Ketter JS, Marzluf GA: Molecular cloning and analysis of the regulation of cys-14+, a structural gene of the sulfur regulatory circuit of Neurospora crassa. Mol Cell Biol. 1988, 8 (4): 1504-1508.PubMed CentralView ArticlePubMedGoogle Scholar
- Foreman PK, Brown D, Dankmeyer L, Dean R, Diener S, Dunn-Coleman NS, Goedegebuur F, Houfek TD, England GJ, Kelley AS, et al: Transcriptional regulation of biomass-degrading enzymes in the filamentous fungus Trichoderma reesei. J Biol Chem. 2003, 278 (34): 31988-31997.View ArticlePubMedGoogle Scholar
- Ilmen M, Saloheimo A, Onnela ML, Penttila ME: Regulation of cellulase gene expression in the filamentous fungus Trichoderma reesei. Appl Environ Microbiol. 1997, 63 (4): 1298-1306.PubMed CentralPubMedGoogle Scholar
- Craig KL, Tyers M: The F-box: a new motif for ubiquitin dependent proteolysis in cell cycle regulation and signal transduction. Prog Biophys Mol Biol. 1999, 72 (3): 299-328.View ArticlePubMedGoogle Scholar
- Kaplan KB, Hyman AA, Sorger PK: Regulating the yeast kinetochore by ubiquitin-dependent degradation and Skp1p-mediated phosphorylation. Cell. 1997, 91 (4): 491-500.View ArticlePubMedGoogle Scholar
- Marzluf GA: Metabolic regulation in fungi. Applied mycology and biotechnology. 2001, Khachatourians GG, Arora DK, 1: 55-72.Google Scholar
- Breton A, Surdin-Kerjan Y: Sulfate uptake in Saccharomyces cerevisiae: biochemical and genetic study. J Bacteriol. 1977, 132 (1): 224-232.PubMed CentralPubMedGoogle Scholar
- Lachance MA, Pang WM: Predacious yeasts. Yeast. 1997, 13 (3): 225-232.View ArticlePubMedGoogle Scholar
- Scheibel T, Bell S, Walke S: S. cerevisiae and sulfur: a unique way to deal with the environment. Faseb J. 1997, 11 (11): 917-921.PubMedGoogle Scholar
- Harman GE, Howell CR, Viterbo A, Chet I, Lorito M: Trichoderma species – opportunistic, avirulent plant symbionts. Nat Rev Microbiol. 2004, 2 (1): 43-56.View ArticlePubMedGoogle Scholar
- Benitez T, Rincon AM, Limon MC, Codon AC: Biocontrol mechanisms of Trichoderma strains. Int Microbiol. 2004, 7 (4): 249-260.PubMedGoogle Scholar
- Sestak S, Farkas V: Metabolic regulation of endoglucanase synthesis in Trichoderma reesei: participation of cyclic AMP and glucose-6-phosphate. Can J Microbiol. 1993, 39 (3): 342-347.View ArticlePubMedGoogle Scholar
- Segers GC, Nuss DL: Constitutively activated Galpha negatively regulates virulence, reproduction and hydrophobin gene expression in the chestnut blight fungus Cryphonectria parasitica. Fungal Genet Biol. 2003, 38 (2): 198-208.View ArticlePubMedGoogle Scholar
- Xue C, Bahn YS, Cox GM, Heitman J: G protein-coupled receptor Gpr4 senses amino acids and activates the cAMP-PKA pathway in Cryptococcus neoformans. Mol Biol Cell. 2006, 17 (2): 667-679.PubMed CentralView ArticlePubMedGoogle Scholar
- Neer EJ, Schmidt CJ, Nambudripad R, Smith TF: The ancient regulatory-protein family of WD-repeat proteins. Nature. 1994, 371 (6495): 297-300.View ArticlePubMedGoogle Scholar