Global transcriptional response to mammalian temperature provides new insight into Francisella tularensis pathogenesis
© Horzempa et al; licensee BioMed Central Ltd. 2008
Received: 16 May 2008
Accepted: 08 October 2008
Published: 08 October 2008
After infecting a mammalian host, the facultative intracellular bacterium, Francisella tularensis, encounters an elevated environmental temperature. We hypothesized that this temperature change may regulate genes essential for infection.
Microarray analysis of F. tularensis LVS shifted from 26°C (environmental) to 37°C (mammalian) showed ~11% of this bacterium's genes were differentially-regulated. Importantly, 40% of the protein-coding genes that were induced at 37°C have been previously implicated in virulence or intracellular growth of Francisella in other studies, associating the bacterial response to this temperature shift with pathogenesis. Forty-four percent of the genes induced at 37°C encode proteins of unknown function, suggesting novel Francisella virulence traits are regulated by mammalian temperature. To explore this possibility, we generated two mutants of loci induced at 37°C [FTL_1581 and FTL_1664 (deoB)]. The FTL_1581 mutant was attenuated in a chicken embryo infection model, which was likely attributable to a defect in survival within macrophages. FTL_1581 encodes a novel hypothetical protein that we suggest naming temperature-induced, virulence-associated locus A, tivA. Interestingly, the deoB mutant showed diminished entry into mammalian cells compared to wild-type LVS, including primary human macrophages and dendritic cells, the macrophage-like RAW 264.7 line, and non-phagocytic HEK-293 cells. This is the first study identifying a Francisella gene that contributes to uptake into both phagocytic and non-phagocytic host cells.
Our results provide new insight into mechanisms of Francisella virulence regulation and pathogenesis. F. tularensis LVS undergoes considerable gene expression changes in response to mammalian body temperature. This temperature shift is important for the regulation of genes that are critical for the pathogenesis of Francisella. Importantly, the compilation of temperature-regulated genes also defines a rich collection of novel candidate virulence determinants, including tivA (FTL_1581). An analysis of tivA and deoB (FTL_1664) revealed that these genes contribute to intracellular survival and entry into mammalian cells, respectively.
Francisella tularensis is a Gram-negative bacterium that is pathogenic to humans . This organism causes mortality in up to 60% of infected individuals if untreated . Based on the potential to weaponize this organism, the Center for Disease Control and Prevention has classified F. tularensis as a Category A biodefense agent . It is therefore vital to understand how this organism responds to environmental and host signals, and how these cues alter expression of virulence determinants. During the course of a natural Francisella infection, this bacterium may transition from an amoeba  or an arthropod host  to colonize human cells. Accompanying this transition, it is likely that chemical and physical signals alert Francisella that it has entered a mammalian host.
The manner by which F. tularensis integrates environmental stimuli to regulate gene expression is fundamental to the success of this organism as an intracellular pathogen. The most well-studied virulence factors of F. tularensis are encoded in the Francisella Pathogenicity Island (FPI) . The amount of one of the virulence proteins encoded in this cluster, IglC, increases in response to growth in macrophages and hydrogen peroxide . In addition, iron limitation has been shown to induce transcription and protein levels of IglACD and PdpB [8, 9] as well as stimulate siderophore production by F. tularensis . Previously, we have shown that differing culture conditions greatly influence host-pathogen interactions and the ability of F. tularensis live vaccine strain (LVS) to activate macrophages .
Prior to the current study, there has only been a single published report characterizing the global transcriptional Francisella response to an environmental cue, specifically iron limitation . Important insights into the regulation of virulence factors like iglC were defined in this analysis. However, numerous genes associated with virulence by other studies are not affected by different iron concentrations [8, 12–15]. Because F. tularensis may transition between hosts, mammalian body temperature is another signal that is likely to be critical for this pathogen.
Pathogenic bacteria that encounter a shift in temperature during their life cycle sometimes respond with enhanced virulence factor expression [16–19]. However, there are discrepancies among the specific groups of genes that are affected by temperature and the mechanism of regulation between organisms. For example, Shigella increases production of its Type III secretion system in response to mammalian temperature . The homologous secretion apparatus in pathogenic Salmonella, however, is not regulated by temperature . Regarding the mechanism of regulation, genes involved in the heat-shock response are often induced at mammalian temperatures relative to those of the environment. This regulation is usually due to the presence of a conserved inverted repeat regulatory structure in the promoter region , or control by a σ32-type heat shock sigma factor . In contrast, the bacterium responsible for whooping cough, Bordetella pertussis, uses a two-component system comprised of BvgS and BvgA to alter transcription of genes in response to temperature. Following induction of bvgAS at 37°C, phosphorylation by BvgS allows BvgA-binding to promoter regions of virulence genes, such as the adhesin, fimX . Given the uncertainty of target temperature-regulated genes and differences in mechanism among bacteria, it is necessary to define the temperature regulon in individual species. Studying gene expression changes induced by a shift to mammalian temperature could provide valuable insight into Francisella virulence.
A few previous studies have investigated the Francisella response to temperature at the molecular level. High temperature (42–44°C) synonymous with heat stress has been shown to have dramatic effects on F. tularensis gene and protein expression [25–27] including increased production of the heat shock proteins GroEL, GroES, DnaK, and ClpB . Heat stress has also been shown to enhance the virulence of a mutant form of F. tularensis LVS . Interestingly, F. novicida has been shown to alter its outer membrane at 25°C versus 37°C by differentially modifying the lipid A component of the lipopolysaccharide . In addition, it has been observed that virulent F. tularensis clinical isolates increase the mannose modification of their lipopolysaccharide when cultivated at temperatures less than 25°C . The results from these previous studies suggest that a global transcript analysis following a shift to mammalian body temperature should reveal gene regulation that is critical to Francisella pathogenesis.
Here we show that a shift from environmental to mammalian body temperature significantly alters the transcriptome of F. tularensis LVS. Many genes that we identified as significantly induced at 37°C have been previously implicated in Francisella virulence, supporting the notion that this temperature shift is important for the regulation of pathogenesis. We provide evidence that the product of a gene encoding the hypothetical protein most profoundly induced by mammalian temperature contributes to virulence and intracellular growth. Moreover, data presented here indicate that a locus up-regulated at 37°C was required for optimal uptake by eukaryotic cells. This is the first F. tularensis gene identified to be involved in the entry of this organism into both phagocytic and non-phagocytic host cells. The results from this study support a model where detection of mammalian body temperature by F. tularensis is important for regulation of physiology necessary for successful infection.
F. tularensisLVS global temperature regulation
Strains, plasmids, and primers used in this study.
F. tularensis subsp. holartica live vaccine strain
LVS with FTL_1581 disruption, HygR
LVS with FTL_1664 (deoB) disruption, HygR
Δ(mcrA)183 Δ (mcrCB-hsdSMR-mrr)173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac Hte [F' proAB lacIqZDM15 Tn10 (TetR) Amy CamR]
F-φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17 (rk-, mk+) phoA supE44 λ-thi-1 gyrA96 relA1
endA1, recA1, gyrA96, thi, hsdR17 (rk-, mk+), relA1, supE44, Δ(lac-proAB), [F' traD36, proAB, laqIqZΔM15]
Francisella shuttle plasmid, ApR, KmR
Helper plasmid for triparental mating, KmR
Francisella shuttle plasmid, HygR
F. tularensis suicide vector, pBBR1 ori, oriT, contains the hyg cassette driven by the groEL promoter from pMP615, oriT, KmR, HygR
pMQ131hyg with the central 560 base pair region of FTL_1581
pMQ131hyg with the central 900 base pair region of FTL_1664 (deoB)
Broad-host-range vector, pC194 ori, contains cloned FTL_1581 along with 600 base pairs upstream and 100 base pairs downstream of this gene, complementing plasmid, ApR, CamR
pFNLTP8 Δnpt, ApR
pF8AX with cat, ApR, CamR
pF8CAT with cloned FTL_1664 (deoB) along with 600 base pairs upstream and 100 base pairs downstream of this gene, complementing plasmid
5'-Phosphorylated – CATTAGCTCGAGGGAAGAGTATGAGTATTCAAC-3'
F. tularensisLVS temperature regulation of genes necessary for infection
We hypothesized that the shift from environmental to mammalian temperature may be used to regulate genes important for infection. Surprisingly, none of the genes encoded in the FPI were significantly up-regulated at 37°C in the microarray analysis. We also confirmed, by immunoblotting, that IglC protein levels were equivalent in bacteria grown at the two different temperatures (data not shown). Upon further scrutiny, we did notice that one gene in the FPI, pdpC (FTL_0116; FTL_1162), was near the statistical threshold for induction at 37°C. We therefore analyzed pdpC transcript levels by Q-PCR as before, which confirmed that this gene was induced at mammalian body temperature (Fig. 2A).
List of genes induced at 37°C shown or implicated to be associated with intracellular growth and/or virulence of F. tularensis.
Description, gene name (if available)
Aspartate carbamoyltransferase, pyrB
Carbamoyl-phosphate synthase large chain, carB
Carbamoyl-phosphate synthase small chain, carA
Pyridoxal/pyridoxine/pyridoxamine kinase, pdxY
Chaperone Hsp90, htpG
Dephospho-CoA kinase, coaE
Pseudogene with homology to miaB
Hypothetical protein with homology to NADPH-dependent FMN reductase
glycine cleavage system P protein, subunit 1, qcvP1
Annotated as transcriptional regulator, homologous to Pantothenate kinase type III, coaX
Conserved hypothetical protein
D-methionine binding transport protein, ABC transporter, membrane and periplasmic protein, metlQ
Conserved hypothetical protein, yleA
protease, GTP-binding subunit, hflX
DJ-1/PfpI family protein
Conserved hypothetical protein
Chaperone protein (heat shock protein family 70 cofactor), grpE
Alanine racemase, alr
transcriptional elongation factor, qreA
Conserved hypothetical membrane protein
SNO glutamine amidotransferase family protein
Pyridoxine/pyridoxal 5-phosphate biosynthesis protein
Succinyl-CoA synthetase beta chain, sucC
Dimethyladenosine transferase, kasugamycin resistance, ksgA
Chaperonin (Hsp60 family), groEL
adenine phosphoribosyltransferase, apt
FTL_1581 is associated with F. tularensisLVS virulence
We disrupted FTL_1581 in LVS, producing strain 1581d, to determine if this gene was associated with F. tularensis virulence. To assess the virulence of 1581d, we utilized a competition assay based on the chicken embryo infection model [36–38]. The chicken embryo produces a robust innate immune response comprised of complement, phagocytic cells, and cytokine production [39, 40]. Here, chicken embryos were infected with a ~1:1 mixture of LVS and 1581d. At days 1, 2, and 3 post-infection, wild-type LVS exhibited superior survival compared to 1581d (P < 0.001 at each time point) (Fig. 4B). This result was not due to a growth defect since 1581d grew identically to wild-type LVS when cultivated in bacterial growth medium (data not shown). In a separate experiment in which chicken embryos were infected only with 1581d, isolates from homogenates were all resistant to hygromycin, indicating that this mutant did not revert to wild type during infection (data not shown). Since 1581d was attenuated compared to LVS, the results suggest the function of the FTL_1581 gene product contributes to F. tularensis virulence.
We wanted to determine if the virulence attenuation of 1581d in the chicken embryo infection model was due to a reduced ability to inactivate complement, as FTL_1581 contained a putative complement resistance domain (Fig. 4A). Therefore, we subjected LVS and 1581d to serum sensitivity assays. There was equivalent survival of wild-type LVS and 1581d when cultured in media containing 20% serum or 20% heat-inactivated serum for up to 20 h (data not shown). To ensure that the serum complement was functional, E. coli DH5α was used as a control. Here, the CFU from the serum-treated E. coli exhibited a reduction of 3 logs relative to the input or heat-inactivated serum groups after 30 min (data not shown). This suggested that the disruption in 1581d does not affect complement resistance.
We determined if FTL_1581 contributed to growth in a macrophage environment. Primary human monocyte-derived macrophages were infected in vitro with either LVS or 1581d. At various time-points, macrophages were lysed, and the lysates were diluted and plated to enumerate viable CFU. We observed attenuated growth of 1581d in macrophages at 24 h post-infection (P = 0.003) (Fig. 4C). When FTL_1581 was complemented in trans in 1581d, wild-type level of growth was restored (Fig. 4C). This complementation confirmed that the reduced intracellular fitness of 1581d was due to the inactivation of FTL_1581, and not due to polar effects or alternate mutations. This experiment (Fig. 4C) also suggested that the attenuation of 1581d in the chicken embryo model (Fig. 4B) was likely due to a defect in intracellular survival. Together, the data presented here implicate FTL_1581, a Francisella ORF induced by mammalian body temperature that has no obvious homologs, with virulence and intracellular survival. Therefore, we propose that FTL_1581 be named temperature-induced, virulence-associated locus A, or tivA.
Involvement of FTL_1664 (deoB) in uptake of F. tularensisLVS
Many of the genes critical for Francisella infection involved metabolism (Table 2), a major COG category induced at 37°C (Fig. 3). This suggested that physiology vital for the success of F. tularensis as a pathogen was regulated by the temperature shift. Therefore, we were interested in determining the contribution of temperature-regulated metabolic genes toward Francisella pathogenesis. Previously, a microarray-based negative selection screen of F. novicida transposon mutants identified the phosphopentomutase, deoB, as a gene contributing to growth and/or survival in mice . In other bacteria, proteins encoded by deoB homologs normally catalyze the reversible reaction between ribose-1-phosphate and ribose-5-phosphate or between deoxyribose-1-phosphate and deoxyribose-5-phosphate [41, 42]. An F. tularensis LVS chromosomal disruption mutant of deoB, FTL_1664, was constructed (strain 1664d). This mutant had a cellular and colony morphology similar to its wild-type parent strain (data not shown). Also, 1664d grew similarly to wild-type LVS in bacterial culture medium (data not shown).
We employed the chicken embryo infection model [36–38] to confirm that deoB contributes to LVS pathogenesis, as it did in F. novicida . Here, chicken embryos that had been infected with F. tularensis LVS 1664d exhibited significantly enhanced survival compared to those infected with wild-type LVS over a 5 day period (P = 0.0254) (data not shown) corroborating the deoB data from F. novicida .
We then qualitatively assessed uptake into phagocytes by microscopy to confirm the quantitative results obtained with CFU measurements. LVS, 1664d, and 1664d/pFTL_1664 were treated with the green fluorescent stain, Syto-9, and then incubated with RAW 264.7 cells, a murine macrophage-like cell line. After a two hour incubation and washes, cells were observed by fluorescence microscopy. Here, RAW 264.7 cells infected with LVS or 1664d/pFTL_1664 exhibited bright green fluorescence (Fig. 5B). In contrast, the RAW 264.7 cells infected with 1664d produced considerably less fluorescence despite comparable inocula to the cultures (Fig. 5B). The results obtained by microscopy suggested the discrepancy in CFU between wildtype and 1664d was not due to killing in the early phagosome. Rather, the levels of fluorescence in Fig. 5B were consistent with the CFU uptake data presented in Fig. 5A and confirmed that the temperature-regulated deoB is important for optimal uptake by mammalian phagocytes.
To determine if deoB contributed to entry into non-phagocytic cells, we exposed the human embryonic kidney cell line HEK-293 to either LVS, 1664d, or 1664d/pFTL_1664. At two hours post-infection, these cells were treated with gentamicin to kill extracellular bacteria followed by extensive washing. Subsequently, HEK-293 cells were lysed and the lysates were diluted and plated to enumerate CFU. Here we observed that 1664d showed reduced entry into the non-phagocytic HEK-293 cells (Fig. 5C). The uptake defect was again rescued by trans complementation (Fig. 5C). These data suggest that deoB contributes significantly to entry into both phagocytic (Fig. 5A and 5B) and non-phagocytic cells (Fig. 5C).
In this paper, we provide evidence that F. tularensis LVS undergoes significant gene expression changes in response to mammalian body temperature (Fig. 1 and 2, Table 2, and Additional files 1 and 2). We hypothesized that genes important for infection of mammals may be induced during transition to this higher temperature. Although some genes, such as ribosomal RNA and tRNA genes, may reflect an increased growth rate at 37°C, our overall hypothesis was supported by the finding that 40% of the protein coding genes induced at 37°C have been shown or implicated to be important for successful Francisella infection (Table 2). In addition, we showed that two specific genes induced at 37°C contribute to the fitness of F. tularensis LVS during infection. While infection of a mammalian host exerts a complex set of signals on F. tularensis in addition to temperature, it is likely that many of the unexplored genes induced at mammalian temperature have a role in pathogenesis of F. tularensis. Differential responses to temperature among virulent and less virulent F. tularensis strains will also provide a focused list of relevant candidate virulence determinants to investigate further. The products of temperature-regulated genes with central roles in physiology and virulence could be targets for novel therapeutics or mutation to generate defined live attenuated vaccines.
One gene induced at 37°C, tivA (FTL_1581), encodes a protein with little overall identity to other proteins. It does, however, contain regions of meager similarity to the domains of proteins involved in the pathogenesis of other bacteria (Fig. 4A). We showed that this gene was necessary for full F. tularensis LVS virulence in the chicken embryo model, most likely because it contributed to optimal replication in primary human macrophages (Fig. 4B and 4C). In a previous high-throughput microarray-based, negative-selection screen for F. novicida genes important for a murine infection, the tivA allele of F. novicida (FTN_0573) was not identified . There are two possible explanations for this discrepancy. There may be intrinsic differences between F. tularensis LVS and F. novicida pathogenesis  and their reliance on tivA. A more probable explanation is that F. tularensis LVS and the more pathogenic F. tularensis Schu S4 contain a single copy of this gene. In contrast, F. novicida has two homologs of tivA in its genome, suggesting the possibility of functional redundancy. These alternate forms (FTN_1103, 52% identity; FTN_1101, 49% identity) may have compensated for a mutation of tivA (FTN_0573) during the negative-selection screen , thereby masking any effects on pathogenesis. This possibility underscores the value of analyzing several different strains when probing F. tularensis pathogenesis.
Strain 1664d, a disruption mutant of deoB, exhibited significant reduction in uptake into mammalian cells (Fig. 5) indicating that this gene's product is involved in a mechanism that enhances entry. It would be advantageous for Francisella to couple expression of a gene important for uptake (deoB) with mammalian temperature, a cue encountered early in infection. Augmenting entry would improve access to the intracellular environment in which F. tularensis proliferates . A previous study showed that pyrB (FTT1665) from F. tularensis Schu S4 was important for invasion in the human HepG2 hepatocellular carcinoma line . However, a Schu S4 mutant of pyrB was not defective in uptake by J774.1 macrophage-like cells , suggesting that the Francisella mechanisms of optimal uptake by phagocytes and invasion into hepatocytes are separate phenomena. In another prior study, mutants of MglA, a key regulator of the FPI , and mutants of six genes controlled by this protein were not defective for cell entry . This suggests that Francisella has evolved separate regulatory mechanisms for enhancing uptake and for intracellular survival. However, based on our data, genes critical for both phenomena are affected by mammalian temperature indicating that this cue is an important signal for multiple regulatory networks in Francisella. The uptake defect we have shown with the deoB mutant in both phagocytic and non-phagocytic cells has not been described previously.
In our system, DeoB may have a direct or indirect role in the uptake of Francisella. DeoB may influence the LPS structure of Francisella, secondarily improving interactions with host cell receptors. Importantly, complement receptors and mannose receptors are crucial for optimal Francisella phagocytosis [47–50]. This model is consistent with the fact that deoB is induced at 37°C and the finding that Francisella LPS structure is different at lower temperature versus mammalian temperature [29, 30]. Alternatively, DeoB may directly promote Francisella entry into host cells as this protein may have an additional function aside from being a phosphopentomutase. Another possibility is that mutation of metabolic genes in pathogenic bacteria can yield pleiotropic effects, resulting in defects in virulence mechanisms, including invasion . Further investigation is necessary to determine the precise mechanism of Francisella DeoB in host cell entry.
The data presented here suggest that some genes important for Francisella during infection are induced by mammalian temperature. Although we showed by Q-PCR that pdpC transcripts were induced at 37°C, none of the other loci in the FPI  were up-regulated at mammalian body temperature. This is consistent with other findings suggesting that IglC protein levels were not induced by a shift from 37°C to 42°C . Because many of the loci in the FPI are essential for intra-amoeba growth , which would occur at lower, ambient temperatures, it seems logical that these genes are not regulated by temperature. Therefore, the virulence associated-genes induced by mammalian temperature that we have identified are on a separate regulon than most of the genes of the FPI. This suggests Francisella possesses an intricate regulatory circuit to maximize its success in diverse environments.
F. tularensis LVS undergoes significant gene expression changes in response to mammalian temperature. This temperature shift is important for the regulation of pathogenesis. Our study characterizes a previously underappreciated environmental cue that regulates the expression of F. tularensis genes associated with virulence in other studies. Importantly, the collection of temperature-regulated genes also defines a rich set of novel candidate virulence determinants, including tivA (FTL_1581). Detailed investigation of tivA and deoB (FTL_1664) revealed unknown or unrecognized roles of these genes in intracellular survival and entry into mammalian cells, respectively.
Bacterial strains and growth conditions
Bacterial strains used in this study can be found in Table 1. All broth cultures were grown with agitation (250 rpm). For general cultivation of Escherichia coli, bacteria were grown at 37°C on LB agar plates, or in LB broth. F. tularensis LVS, a model organism for tularaemia, was used in this study. For F. tularensis LVS strains, frozen stock cultures were streaked onto chocolate II agar plates and incubated at 37°C, 5% CO2 for 2–4 days. These bacteria were subsequently used to inoculate broth cultures. For experiments assessing the effect of temperature on transcript levels, Chamberlain's chemically defined broth medium (CDM)  was inoculated with LVS and incubated at 37°C overnight. This start-up culture was used to inoculate fresh CDM (2 ml start-up culture into 25 ml fresh broth) and incubated at 26°C for 24 h. Following this incubation, the optical density (OD600) of this culture was recorded and RNA was extracted. For the temperature shift, 5 ml of the same culture was diluted into 25 ml fresh CDM and incubated at 37°C until attaining an OD600 comparable to the 26°C culture (typically ~6 h). When the 37°C culture reached the desired OD600, RNA was harvested. This strategy of growing bacteria to comparable OD600 prior to RNA extraction was similar to a previous study assessing global temperature regulation in Group A Streptococcus  and mitigates the effects of growth phase on the subsequent analysis.
For macrophage and chicken embryo infections, LVS strains were grown in TSBc (trypticase soy broth [Becton, Dickinson and Company] supplemented with 0.1% L-cysteine hydrochloride monohydrate) at 37°C while shaking at 250 rpm. When required, antibiotics were added to the media at the following concentrations: ampicillin at 150 μg/ml for E. coli; kanamycin at 35 μg/ml for E. coli and 10 μg/ml for F. tularensis LVS; chloramphenicol at 5 μg/ml for F. tularensis LVS; polymixin at 100 μg/ml; and hygromycin at 200 μg/ml.
The rationale for using CDM for the transcriptional analysis, while TSBc was used for all other experiments is two-fold. Using CDM for general cultivation of LVS is not practical because CDM is an aqueous mixture of 22 nutrients, some of which do not have a long shelf-life. Secondly, CDM was selected for transcriptional analyses to benefit future studies to analyze synergistic effects of temperature and media components.
Immediately upon removal from the shaking incubator, six ml of broth culture were mixed with 18 ml TriReagent LS (Molecular Research Center). Chloroform (4.8 ml) was added to this material, and the aqueous phase was subsequently separated by centrifugation in a Phase Lock Heavy tube (Eppendorf). RNA was precipitated from this aqueous layer with isopropanol, followed by centrifugation. Pelleted material was washed in 80% ethanol and resuspended in nuclease-free water. This RNA-containing mixture was treated with DNase (Turbo DNA-free, Ambion), and then precipitated using ammonium acetate and ethanol. RNA quantity was measured spectrophotometrically, and quality was assessed using an Agilent Bioanalyzer.
Custom Agilent Francisella microarrays designed using the eArray framework were used in this study. All predicted open reading frames, including pseudogenes, were included for F. tularensis subsp. holarctica LVS, F. tularensis subsp. tularensis (SchuS4), F. tularensis subsp. holarctica OSU18, F. novicida U112 and the Francisella plasmids, pOM1, and pFNL10. Individual genes from LVS, SchuS4, and OSU18 were spotted in duplicate on this array, whereas genes from the other strains and plasmids were printed as single copies. cDNA was synthesized from RNA by reactions using random hexamers (Invitrogen) and MMLV (Agilent). The cDNA target was labelled with Alexa Fluor 555 conjugated dUTP according to manufacturer's protocol (AF555-aha-dUTP; Invitrogen). Labelling reactions were incubated at 40°C for 3 hours. After cDNA synthesis, RNA was hydrolyzed with with NaOH and EDTA, and subsequently, the pH was neutralized with an equivalent volume of HCl. The labelled cDNA was precipitated using isopropanol and ammonium acetate and washed with 80% ethanol. 0.5 μg of labelled cDNA was hybridized to custom Agilent 8 × 15 K microarrays according to the protocol of the manufacturer and incubated at 60°C for 18 hours in a rotary oven. Following hybridization, arrays were washed with Agilent wash buffers before being scanned on an Agilent microarray scanner. At least 3 separate RNA extractions were used for each condition tested by microarray (26°C or 37°C-grown bacteria).
Gene Expression Data Analyzer (GEDA; http://bioinformatics.upmc.edu/GE2/GEDA.html) was utilized for analysis of the microarray data . This online software package was used for minimum mean ratio array normalization followed by log2 transformation. Data were grouped into two categories, cultivated at 26°C and shifted to 37°C, and analyzed for significance with the J5 statistical test . The J5 metric was designed for data sets composed of limited replicates, thereby reducing the chance of generating false positives . Genes were considered to be significantly differentially regulated if they had a J5 score greater than 2. Additional files 1 and 2 consist of lists showing both the J5 value and fold change for genes that were significantly up- or down-regulated upon a shift to mammalian temperature. Normalized, log2 transformed intensity values for genes that were differentially expressed were input into GenePattern  and data are presented after hierarchical clustering (Pearson correlation).
Categories assigned for clusters of orthologous groups were identified for each significantly up- or down-regulated gene based on the annotation assigned from the F. tularensis holarctica genome (accession, NC_07880). Specific clusters of orthologous groups categories were consolidated into general categories as follows: J, K, L, and RNA genes were combined into "Transcription/translation/DNA replication/RNA"; P, C, G, E, F, H, I, and Q were merged into "Transport and metabolism"; categories N and U were combined into "Cellular functions/trafficking/secretion"; and categories R, S, and uncategorized genes were classified as "Unknown". All other clusters of orthologous groups categories were reported as they were originally assigned.
Quantitative real time PCR
One μg of total RNA was used as a template for cDNA synthesis catalyzed by Superscript III (Invitrogen). Diluted cDNA was used as a template for real time reactions containing primer sets (designed by Primer 3  for the desired genes) and SYBR Green Supermix (BioRad). These reactions were carried out on a BioRad IQ5 real time machine. The bacterial 50S ribosomal protein L24 (FTL_0247) was used as an internal reference, as it was observed to have no change in expression following a shift to 37°C according to the microarray analysis (data not shown). The quantitative real time PCR data are presented as log2 transformed fold change values (37°C versus 26°C).
Construction of plasmids
Plasmids and primers used in this study can be found in Table 1. The disruption plasmids, pMQ131hyg1581d and pMQ131hyg1664d were constructed using the following procedures. First, the PvuI/SpeI fragment of pMP615 containing hyg under the control of the groEL promoter was subcloned into pMQ131 (R. Shanks and G. O'Toole, unpublished data) that had been digested with PvuI/XbaI which produced pMQ131hyg. The internal 560 base pairs of FTL_1581 were amplified by PCR using the primers 1581_560F and 1581_560R and this amplicon was initially TA cloned into pGEM-T (Promega). The KpnI/BamHI fragment of this construct containing the internal portion of FTL_1581 was subcloned into pMQ131hyg that had been digested with these same enzymes, which produced pMQ131hyg1581d. Similarly, the internal 900 base pairs of FTL_1664 were amplified by PCR using the primers 1664_900F and 1664_900R and this amplicon was initially TA-cloned into pGEM-T (Promega). Subsequently, this fragment internal to FLT_1664 was subcloned (KpnI/BamHI) into pMQ131hyg, producing the plasmid pMQ131hyg1664d.
The FTL_1581 complementing plasmid (pFTL_1581) was constructed using the following procedures. The primers 1581_clone_up and 1581_clone_down were used to amplify 600 base pairs upstream, the entire FTL_1581 open reading frame, and 100 base pairs downstream of this gene by PCR. This amplicon encompassed the predicted native FTL_1581 promoter, this gene's coding region, as well as the predicted transcriptional stop (data not shown). Initially, this amplicon was TA-cloned into pGEM-T (Promega). The SacI/SacII fragment of this construct containing FTL_1581 was subsequently subcloned into pMQ2 (R. Shanks and G. O'Toole, unpublished data) that had been digested with these same enzymes. This yielded pFTL_1581.
The FTL_1664 (deoB) complementing plasmid (pFTL_1664) was constructed using the following procedures. Initially, the KanR gene from pFNLTP8 was deleted using an inverse PCR strategy . The primers F8AgeI and F8XhoI were used to amplify pFNLTP8. Following PCR, template DNA was digested with DpnI leaving only amplified product. This amplicon was self-ligated, producing pF8AX. The cat194 gene was amplified by PCR using the primers XhoICAT and AgeICAT with pMQ2 as a template, and this amplicon was TA-cloned into pGEM-T (Promega). The Age I/XhoI fragment of this construct that encoded the cat194 gene was subcloned into pF8AX using these same enzymes producing pF8CAT. 1664_clone_up and 1664_clone_down were used to amplify 600 base pairs upstream, the entire FTL_1664 open reading frame, and 100 base pairs downstream of this gene by PCR. This amplicon encompassed the predicted native FTL_1664 promoter, this gene's coding region, as well as the predicted transcriptional stop (data not shown). Initially, this amplicon was TA-cloned into pGEM-T(Promega). The BamHI/KpnI fragment of this construct containing FTL_1664 was subsequently subcloned into pF8CAT that had been digested with these same enzymes. This yielded pFTL_1664.
Construction of the FTL_1664 and FTL_1581 mutants
F. 0tularensis LVS disruption mutants of FTL_1664 and FTL_1581 were constructed as previously reported . A homologous recombination between the LVS chromosomal copy of FTL_1581 and the internal FTL_1581 fragment in pMQ131hyg1581d would disrupt the coding sequence of 38 amino acids at the C-terminus. Also, homologous recombination between chromosomal FTL_1664 and pMQ131hyg1664d would disrupt coding sequence for the C-terminal 56 amino acids of the encoded protein. Disruption constructs (either pMQ131hyg1664d or pMQ131hyg1581d) were mobilized into F. tularensis LVS by triparental mating. Mating mixes were composed of LVS (109 CFU), E. coli/pRK2013 (107 CFU), and either E. coli/pMQ131hyg1664d or E. coli/pMQ131hyg1581d (107 CFU). Similarly to the previously described Francisella conjugation methodology , the mating mixture here was plated on LB agar plates, and incubated at room temperature for 18 hours. Cells were resuspended in phosphate buffered saline (PBS) and spread onto chocolate II agar plates containing polymyxin (100 μg/ml) and hygromycin. Hygromycin-resistant colonies were screened by PCR using primers specific for either FTL_1581 or FTL_1664 and the vector-portion of pMQ131hyg1581d or pMQ131hyg1664d. The F. tularensis LVS FTL_1664 and FTL_1581 disruption mutants (FTL_1664::pMQ131hyg1664d; FTL_1581::pMQ131hyg1581d) are referred to as strains 1664d and 1581d respectively. Mobilization of complementation constructs into the disruption mutants was accomplished by electroporation as previously described .
Complement resistance assay
Bacteria (1.5 × 109 CFU) were incubated in solutions of PBS with 20% serum (human serum [Gemini Bioscience]) or 20% heat-inactivated serum at 37°C while shaking at 250 rpm. Serum was incubated at 56°C for 30 minutes to inactivate complement. At certain time-points, cell suspensions were diluted and plated to enumerate CFU.
Chicken embryo infections
Chicken embryos were infected with F. tularensis LVS, 1664d, and 1581d as previously described [37, 38]. Whiteleghorn chicken eggs (fertilized, specific pathogen free) were purchased from Charles River Laboratories, North Franklin, CT, USA. Eggs were incubated at 37°C with gentle rocking and humidity for one week prior to infection. Following the initial 7 day incubation, those without a live embryo were discarded. For infections, the surface of the egg shell was sterilized with 70% ethanol. The egg shell membrane was exposed and removed after introducing a small hole in the shell. Bacteria suspended in PBS (100 μl) were then injected beneath the chorioallantoic membrane. The hole in the egg shell was covered with transparent tape and subsequently eggs were incubated as previously described. On a daily basis, eggs were candled to assess viability for 6 days. As previously reported , embryos that expired within 24 h of the infection were presumed to have experienced lethal trauma during inoculation, and were removed from the experiment. Survival differences in chicken embryo infections were analyzed with the log rank test in GraphPad Prism 5.
Competition studies in the chicken embryo infection model were conducted similar to the single infections described above. Here chicken embryos were infected with a mixture of LVS and 1581d (1:1 based on OD600, ~105 total bacteria). Actual input (6.5 × 105 total bacteria) was determined by diluting the inoculum followed by plating to enumerate viable bacteria. Three infected, viable eggs were sacrificed at 1, 2, and 3 days (9 total eggs) post infection. Egg contents were homogenized with an OMNI tissue homogenizer, and serial dilutions of the homogenates were plated on chocolate II agar plates with or without antibiotic. The total number of 1581d (plates with antibiotic) was subtracted from the number of total bacteria (plates without antibiotic) to determine the amount of viable wild-type LVS. Competition ratios (1581d: LVS) were normalized to the input to account for small differences in the inocula, as has been done previously [58, 59]. Competition ratios were analyzed for statistical significance by Chi square.
Mammalian cell infections
Human monocytes were differentiated into macrophages by in vitro culture as has been described previously [11, 57, 60]. Buffy coats from blood donations (Central Blood Bank, Pittsburgh, Pennsylvania) served as the source of monocytes. Monocytes were purified using Ficoll gradients (Amersham Biosciences) to isolate PBMCs, Optiprep gradients (Axis-Shield) to enrich for monocytes, and panning on plastic to further purify monocytes (final purity >95% based on microscopy). Cells were cultured in 60 mm culture dishes for 7 days at 37°C with 5% CO2 in 7 ml of DMEM (Invitrogen) supplemented with 20% FCS (Invitrogen), 10% human serum (Gemini Biosciences), 25 mM HEPES (Gibco), and 1% Glutamax (Invitrogen) [11, 57, 60]. Macrophages were detached from the culture dish on day 7 using a lidocaine/EDTA solution (5 mM EDTA and 4 mg/ml lidocaine in PBS pH 7.2). For monocyte-derived dendritic cells, human monocytes were seeded at a density of 1 × 106 cells/ml in 24 well plates (Costar) in complete RPMI [10% FCS, 25 mM HEPES, 1% non-essential amino acids, 1% sodium pyruvate, 1% Glutamax and 0.1% 2-mercaptoethanol (all from Gibco)] supplemented with 1000 U/ml GM-CSF and 1000 U/ml IL-4 (both from eBioscience) and incubated at 37°C with 5% CO2. On day 3 of culture, 10% of the media was replaced with fresh complete RPMI supplemented with GM-CSF and IL-4 and non-adherent cells were harvested at day 5. For in vitro infections, all primary cells were washed and resuspended in DMEM supplemented with 1% human serum, 25 mM HEPES, and 1% Glutamax and then plated onto Primaria-coated 96-well culture dishes (Becton, Dickinson and Company) at a density of 5.0 × 104 cells per well.
Murine macrophage-like RAW 264.7 cells (ATCC number TIB-71) were routinely cultured in DMEM supplemented with 10% fetal calf serum, 25 mM HEPES, and 1% Glutamax with 100 U/ml penicillin-streptomycin. Two days prior to infection, the medium for these cells was replaced with DMEM supplemented with 10% human serum, 25 mM HEPES, and 1% Glutamax, and infections were carried out using this same medium.
HEK-293 cells (ATCC number CRL-1573), a non-phagocytic cell line , were cultivated in DMEM supplemented with 10% fetal calf serum, 25 mM HEPES, and 1% Glutamax with 100 U/ml penicillin-streptomycin. One day prior to infection, HEK-293 cells were seeded in 96-well plates at a concentration of 5 × 104 cells per well in this same medium devoid of antibiotic and infections were carried out in this same medium.
For infection experiments, bacterial cultures were adjusted to an OD600 of 0.3 (approximately 1.5 × 109 CFU/ml) and diluted to attain a multiplicity of infection (MOI) of 500, which typically yields infection rate >80% after a 2 hour co-incubation [11, 62]. Alternatively, macrophages were suspended in DMEM supplemented with 10% human serum, 25 mM HEPES, and 1% Glutamax and transferred to culture dishes before infection. Prior to administration of the bacteria onto the macrophages, the F. tularensis strains were incubated at 37°C with 5% CO2 for 20 min in this same medium. Macrophages were infected with bacteria that had been diluted to an MOI of 40. This alternative infection protocol used a lower MOI because it took advantage of the complement-mediated uptake of F. tularensis into macrophages . Comparable results were obtained regardless of which infection protocol was used. Actual MOIs were measured by plating serial dilutions of inocula on chocolate II agar plates.
For intracellular CFU enumeration, mammalian cells were exposed to the initial bacterial load for 2 hours at 37°C with 5% CO2 and then incubated with gentamicin (20 μg/ml) for 20 min to kill extracellular bacteria. The cells were washed with warm Hank's balanced salt solution and then incubated at 37°C with 5% CO2 for another 22–48 h with fresh culture media. At the indicated time-points post-infection, mammalian cells were lysed with 0.02% sodium dodecyl sulfate. The 2 hour time point was used to determine entry similarly to a prior study . Serial dilutions of the lysates were plated on chocolate II agar plates for enumeration of viable bacteria. CFU counts were converted to log10 values and analyzed by the Student's t-Test to determine statistical differences. For uptake analysis, CFU were normalized to actual MOI to account for minor variations in the density of inocula .
For fluorescence microscopy, bacteria were stained with 10 μM Syto 9 (Invitrogen) for 15 min and washed three times with PBS prior to infection. Here, stained cells were diluted in DMEM supplemented with 10% human serum, 25 mM HEPES, and 1% Glutamax, and were incubated at 37°C with 5% CO2 for 20 min. These bacteria were used to inoculate RAW 264.7 in this same medium at an MOI of 10.
We thank John W. Robison for technical assistance, and James Carroll, and Cory M. Robinson for insightful discussion. The authors thank Deanna Tarwacki for assistance with dendritic cell cultivation and infections. We also thank Francis E. Nano for helpful correspondence regarding the chicken embryo challenge model. We thank Thomas Zahrt and Martin Pavelka for providing us with plasmids and Karen Elkins for providing F. tularensis LVS. This work was funded by the National Institutes of Health grants AI074402 and AI050018. JH is a recipient of T32 AI060525, "Immunology of Infectious Disease."
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