Bioinformatics analysis of the locus for enterocyte effacement provides novel insights into type-III secretion
© Pallen et al; licensee BioMed Central Ltd. 2005
Received: 21 December 2004
Accepted: 09 March 2005
Published: 09 March 2005
Like many other pathogens, enterohaemorrhagic and enteropathogenic strains of Escherichia coli employ a type-III secretion system to translocate bacterial effector proteins into host cells, where they then disrupt a range of cellular functions. This system is encoded by the locus for enterocyte effacement. Many of the genes within this locus have been assigned names and functions through homology with the better characterised Ysc-Yop system from Yersinia spp. However, the functions and homologies of many LEE genes remain obscure.
We have performed a fresh bioinformatics analysis of the LEE. Using PSI-BLAST we have been able to identify several novel homologies between LEE-encoded and Ysc-Yop-associated proteins: Orf2/YscE, Orf5/YscL, rORF8/EscI, SepQ/YscQ, SepL/YopN-TyeA, CesD2/LcrR. In addition, we highlight homology between EspA and flagellin, and report many new homologues of the chaperone CesT.
We conclude that the vast majority of LEE-encoded proteins do indeed possess homologues and that homology data can be used in combination with experimental data to make fresh functional predictions.
Type-III secretion is one of five different types of protein secretion employed by Gram-negative bacteria to move proteins from the cytoplasm across two membranes to the external milieu [1–5]. Any given type-III secretion system (T3SS) consists of a multi-protein complex that spans both the inner and outer membranes and the periplasm so that proteins are delivered to the exterior in an ATPase-dependent fashion without a periplasmic intermediate. Type-III secretion systems can be classified into two major groups: those associated with flagellar biosynthesis and those associated with interactions between bacteria and eukaryotic cells . Type-III secretion is thus central to our understanding of bacterial motility, virulence, symbiosis, and ecology. Type-III secretion also provides an attractive drug and vaccine target  and has been exploited in the biotechnology arena as a antigen delivery system [7, 8]
When applied to biological sequences, homology is defined as similarity that arises because of descent from a common ancestor . Assignments of sequence homology have a predictive value, in that sequence homology implies structural homology, and, less certainly, functional similarity. Although homologous sequences can diverge in function, or be recruited to a different physiological role or cellular compartment, the discovery of homology allows functional assignments to be transferred from a protein or gene that has undergone experimental investigation to a protein or gene that has not been studied in the laboratory (albeit tentatively and allowing for various common pitfalls in this process ). Any such functional assignment should always be treated as a working hypothesis rather than as an established fact. However, this approach can lead to new predictions about biological function that can then be tested in the laboratory, informing an existing programme of experimental work, or even opening up an entire line of enquiry where none existed before [22, 23]. At its most basic, an assertion of homology calls to our attention the "null hypothesis" – that proteins with homologous sequences should behave in similar ways – and, whenever this does not appear to be true, provokes the question, why are these proteins behaving differently? Searches for homology between sequences are reliable only when the search program provides statistics that allow one to estimate how likely it is that randomly composed sequences could yield alignment scores at least as high as that obtained between the two sequences in question (an example of such a statistical evaluation is the e value reported by BLAST ).
When the complete sequence of the LEE was first reported, an attempt was made to create a standard rational nomenclature based on experimental findings and functional predictions. E. coli-secreted proteins were given the generic name Esp (EspA, EspB, EspD) . Components of the secretion apparatus that had obvious homologues in the better-characterized plasmid-encoded Ysc-Yop T3SS of Yersinia sp. were given the prefix Esc and the same suffix as their Yersinia homologues (EscC, EscD, EscJ, EscN, EscR, EscS, EscT, EscU and EscV). Proteins that apparently lacked Yersinia homologues, but appeared to be involved in type III secretion, retained the designation Sep (for "secretion of E. coli proteins"): originally SepZ for rOrf9 and SepQ for Orf17, but with the designations SepD for rorf6 and SepL for Orf23 subsequently seeing widespread use. The chaperone for the secretion of EspD was named cesD ("chaperone for E. coli secreted protein D"). The intimin gene was named eae (for "E. coli attaching and effacing") and the intimin receptor named Tir (for "translocated intimin receptor"). Since these original designations were made, several other LEE-encoded proteins have been characterized and re-named according to these conventions ((EspF, EspG, EspH; CesF, CesAB/CesA, CesD2), while some others have acquired names outside the original nomenclature, based on functional properties (Ler, Map, GrlR, GrlA) [25–33]
Summary of known characteristics of LEE-encoded genes and their associated proteins, with novel homology-based functional predictions. New names as suggested in body text of this article. Summary of effects on gene expression, type-III secretion and virulence in mice drawn from Deng et al . Yeast two-hybrid results drawn from Creasey et al . Predictions drawn from combination of homology data and cited works. T3S: involved in type-III secretion.
Gene (new name)
Effect of deletion mutation on secretion
Effect on pedestal formation / virulence
New functional prediction
SdiA-regulated domain (NHL repeats)
Might form heterodimers with H-NS, StpA
YscE, PscE, SsaE, CV2595, YPO0259
Interacts with C-terminus of SepL; Functionally important conserved residues can be identified by scrutiny of homologues
± EspA, EspB
Chaperone for EspA and EspB
YPO0264, SsaK, CV2589
Functionally important conserved residues can be identified by scrutiny of homologues
Binds to and regulates activity of ATPase EscN
EscR, EscS, EscU, SepZ EspD
Physically associated in the basal body with EscU in a 1:1 ratio; C-terminus is cytoplasmic 
EscrR, EscI, EspD
Physically associated in the basal body with EscT in a 1:1 ratio ; cleaved at NPTH motif ; regulates substrate specificity of secretion system .
lytic transglycosylase IagB, IpgF
Lytic transglycosylase needed to open gap in peptidoglycan for assembly of secretion system ; potential drug target
Will work in concert with GrlA
Bongori regulator, CaiF
Will work in concert with GrlR
Other TPR chaperones
Chaperone for EspD
TPR chaperone 
YscC, MxiD, InvG
Interacts with peptidoglycan , Dsb-dependent domain missing
Switches translocator/effector secretion
Works in concert with SepL
YscJ, PrgK, FliF
EscD and EscJ form ring-like structure in inner membrane [66, 162, 163]
PrgJ, MxiI, YscI
Forms inner rod within base of needle complex [65, 68-70].
Might play a role in switching translocator/effector secretion
CesT, Tir 
Forms hexameric complex
C-terminal cytoplasmic domain might play a role in gene regulation [164, 165].
Secretion of translocators
Forms ring within basal body ; interacts with EscL 
Chaperone for EspF
Map, EspF, Tir, CesT
Chaperone for Tir
Possible regulatory role, by analogy with ExsC?
YscD: FHA and BON domains
EscD and EscJ form ring-like structure in inner membrane [66, 162, 163]; BON domains mediate binding to phospholipids; FHA domain mediates protein-protein interactions and signalling
Switches translocator/effector secretion
single protein mediating effects of YopN and TyeA; internal interaction between YopN- and TyeA-like moieties; also a translocated effector?
Half-flagellin model (see text); dimerizes before polymerizes; lacks D0 and D3 domains
EspD, EscRSU, CesD, rORF1, SepZ
Chaperone for EspD
LcrR will bind YopB
Major needle component
YPO0261, SsaI, H, CV2586
Functionally important conserved residues can be identified by scrutiny of homologues
Results and discussion
The conserved type-III secretion apparatus
Several LEE-encoded proteins have already been assigned Esc designations to reflect their similarities to the Ysc proteins in the Yersinia Ysc-Yop system . BLASTP-based comparisons of the predicted components of basal complex confirms significant near-full-length homology between EscC, EscD, EscJ, EscN, EscR, EscS, EscT, EscU and EscV and the respective Ysc proteins and homologues (Figure 1, Table 1), although, confusingly, YscV is more commonly known as LcrD, and EscD is sometimes termed Pas [1, 47, 48]. In the cases of EcsN, EscR, EscS, EscT, EscU and EscV, homology clearly extends to components of the flagellar apparatus . These unequivocal homologues suggest that observations on these proteins in other systems can safely be generalised to the LEE-encoded system – as has been confirmed in several recent papers [49, 50] – and can be used to frame novel hypotheses about their function (Table 1, Figure 1). However, it is important to remain on guard for minor but, perhaps, important differences between systems: for example, the C-terminal ~100-residue domain from YscC, which is thought to house two DsbA-dependent disulfide bridges is missing from EscC and many other homologues .
The missing EscL and EscQ proteins
A lytic trans-glycosylase: a novel drug target?
Both domain searches and PSI-BLAST searches show that rOrf3 encodes a lytic transglycosylase. Similar enzymes, which occur in type II, type III and type IV secretion systems, are responsible for enlarging gaps in the peptidoglycan meshwork to allow efficient assembly and anchoring of supramolecular transport complexes in the cell envelope . The presence of a dedicated transglycosylase within this type-III secretion system provides an obvious target for the development of anti-microbial agents that specifically inhibit type-III secretion, particularly as structural data is available for homologous enzymes [59–61]. We propose that rOrf3 be re-named EtgA for E. coli transglycosylase. Curiously, we have been unable to find a homologue of EtgA in the Ysc-Yop system.
Despite its name, EscF, a major component of the needle, does not show significant similarity to YscF on a simple BLASTP search (19% identity; e value of only 0.88), but the unimpressive significance score can be explained by the short length of the sequence (only 73 residues); the e value rises to a more respectable 0.003 within two iterations of a PSI-BLAST search. Furthermore, it is clear from published experimental work that YscF and EscF play equivalent roles as needle components [62–64].
In the Spi-1 and Mxi-Spa systems, two small proteins are associated with the needle – PrgI/PrgJ and MxiH/MxiI respectively [65–68]. There is experimental evidence from both systems to suggest that only one of the proteins (PrgI/MxiH) is the major subunit component of the needle, while the function of the second protein (PrgJ/MxiI) was, until recently, unclear – it was initially thought to form a cap for the needle [65, 68, 69]. However, the function of PrgJ (and by extrapolation, of its homologues) has now been elucidated – it forms the inner rod within the base of the needle .
Homology between EspA and Flagellin
Firstly, surprisingly, the coiled-coil domain at the C-terminus of EspA appears to correspond not to the coiled-coil at the extreme C-terminus of flagellin, which forms one half of the flagellin D0 domain, but instead to the C-terminal portion of the D1 domain (CD1) [75, 76]. In other words, EspA appears to lack a D0 domain. In the flagellar filament, D0 forms the inner tube, while D1 forms the outer tube . Although D0 interactions are important in filament stability, mutant flagellins that lack the D0 domain can still assemble into straight filaments, albeit with a structure, termed Lt, distinct from the native flagellar filaments . It is thus entirely plausible that the EspA pilus is assembled without the need for a D0 domain and resembles the Lt filament structure. One problem with this is that, unlike the native flagellar filament and the EspA filament, the Lt filament appears to lack a central pore. However, this is probably an artefact (it is hard to see how flagellin monomers could be exported without a pore) and by analogy with flagellin , one could predict that the central pore in the EspA filament is lined by conserved polar residues close to extreme C-terminus of EspA (e.g. Lys-192).
Secondly, EspA appears to lack a D3 domain (and probably also anything homologous to the D2 domain, although it must contain an analogous surface-exposed domain). This fits in with the observation that D3 is highly variable in size and in sequence among flagellins and is not essential for flagellar filament formation in Salmonella . However, it is interesting to note that two EspA homologues from Chromobacterium violaceum possess a central insertion, which is likely to fold into an extensive surface-exposed domain, perhaps analogous to the flagellar D3 domain .
A third, crucial point concerns the role of the coiled-coil domains in EspA. The coiled-coil domains at the N- and C-termini of flagellin were initially thought to facilitate filament assembly by mediating inter-molecular interactions between neighbouring flagellin subunits [79, 80]. Based on the hypothesis that coiled coils might play an analogous role in the assembly of the EspA pilus [72, 81], mutagenesis experiments were performed and showed that the C-terminal coiled coil domain of EspA is required for filament assembly . However, the interpretation of these experiments must now be revised in the light of the flagellar structure and homology data. When the flagellar filament structure was solved, it became clear that, contrary to expectation, the coiled-coil domains mediated interactions within subunits, rather than between them . Thus, by extension, it is likely that, for both EspA and flagellin, the coiled-coil interaction is a necessary but not sufficient requirement for filament assembly.
Coiled-coil prediction programs and PSI-BLAST searches suggest that, in addition to lacking the D0 and D3 domains, EspA lacks any homologue of the N-terminal helix (ND1) of the coiled-coil that forms the D1 domain. However, recent structural information on EspA complexed with its chaperone CesA indicates that EspA contains a short N-terminal coiled-coil domain in addition to the already recognized much longer C-terminal coiled-coil domain . It is thus possible that EspA folds up as a "mini-flagellin", so that together these two coiled-coil domains mediate an intra-subunit interaction similar to that seen in the flagellar monomer. However, this does not fit well with the apparent discrepancy in length between the two domains. An alternative possibility is that EspA acts as "half-a-flagellin", so that it dimerizes through an inter-subunit coiled-coil interaction, and then the dimer polymerizes, like flagellin, through hydrophobic interactions but between dimers rather than monomers.
The "mini-flagellin" and "half-flagellin" models could be distinguished in the laboratory. For example if the half-flagellin model holds true, it should be possible through mutagenesis to identify mutations that disrupt polymerisation but leave the coiled-coil-mediated dimerization potential intact (an approach that might make it possible to grow crystals for structure determination). Furthermore, it should be possible to distinguish dimerization from subsequent polymerisation by physicochemical methods. Finally, under this model, there is a potential for the formation of heterodimers – an idea supported by the existence of four C. violaceum EspA homologues, encoded by two pairs of adjacent genes. In each pair, the first gene encodes a short conventional EspA homologue, while the second gene encodes a long EspA homologue containing a central D3-like insertion . According to the half-flagellin model, the short and the long EspA-like proteins would have the potential to form heterodimers, which would then polymerise into a filament. This prediction could be tested experimentally, e.g. by showing that these EspA homologues form long/short dimers but do not engage in self-self interactions.
The Toll-like receptor 5 (TLR5) recognizes the D1 domain of flagellin . Although EspA lacks several of the residues identified as critical in binding (data not shown), the overall conservation of the CD1 domain raises the question of whether TLR5 might recognize EspA. However, recent experimental investigations suggest it does not (K. D. Smith, personal communication). Yet, this in turn raises the question of whether this lack of interaction is the result of genetic drift or of positive selective pressure on EspA to avoid recognition by TLR5.
PSI-BLAST searches with EspB prove unhelpful, as the compositional bias of the protein, particularly in its coiled-coil domains, attracts numerous simple-sequence proteins, even when a filter and composition-based statistics are used [81, 85]. However, a comparison of domain architecture and genetic location with translocation-pore proteins/genes from other systems (e.g. SipC, IpaB, YopD) suggests functional and structural similarities. PSI-BLAST searches with EspD prove more fruitful, especially when composition-based statistics are employed – they reveal significant similarities to several other T3SS translocon-pore proteins. For example, when EspD is used as a query sequence for a PSI-BLAST search on the NCBI NR database under default settings, YopB is found in the first round with an e value (0.001) within the PSI-BLAST threshold, while SseC achieves significance (4e-07) on the second PSI-BLAST round.
Homologies and Gene fusions in the SepL/YopN-TyeA family
This new-found homology also fuels two predictions: (i) the C-terminal residues 268–351 of SepL, corresponding to TyeA, interact with the residues immediately upstream (229–267) in the region of SepL corresponding to YopN residues 242–280; (ii) the interaction between YopN and TyeA must occur in a 1:1 ratio – this could be confirmed by creating a yopN-tyeA fusion that lacks a start codon for tyeA, and then showing that this construct could complement a yopN/tyeA mutant to give normal type-III secretion.
Further insights into type-III secretion arise when additional PSI-BLAST searches are applied to the homologues retrieved when SepL is used as the query sequence. Firstly, it is clear that the Ysc-Yop system is unusual in separating out the two SepL-related sequences into two proteins. This arrangement occurs only in systems closely related to the Ysc-Yop system (i.e. T3SSs from Pseudomonas aeruginosa, Photorhabdus luminescens, Aeromonas salmonicida, Vibrio parahaemolyticus); in other systems, there is a single protein corresponding to a YopN-TyeA chimaera (e.g. SsaL in Spi-2, HrpJ in Erwinia and related species, BopN in Bordetella, CopN in Chlamydia ). Secondly, through careful use of PSI-BLAST, it is possible, through repeated searching, to establish unequivocal homology between SepL-like proteins from each of the well-characterised animal-pathogen-related T3SSs – not just between SepL and YopN-TyeA, but also encompassing InvE from the Spi-1 system and MxiC from the Shigella system (Figure 8). This implies that functional assignments for any one of these proteins should be generalisable to all the others – a prediction borne out by the similar phenotypes ascribed to these proteins, which all appear to play a role in regulating the translocation of effectors [86, 87, 93–98]. Indeed, it is even possible that some reported differences in phenotype (e.g. the surface location of YopN versus the apparent cytoplasmic location of InvE; the fact that YopN appears to control secretion of all Yops, whereas InvE is more selective in controlling secretion of translocators rather than effectors ) might represent idiosyncrasies of experimental approach than genuine biological differences. However, even if these do represent true differences, this begs the question: how and why have homologous proteins come to acquire such divergent functions?
SepL interacts with another protein of unknown function SepD . Unfortunately, homology searches afforded no insights into the function of SepD. However, given that sepD is located next to escC in the LEE, while yscB occupies an analogous position (next to yscC) in the ysc cluster, it is tempting to speculate that they might play similar roles. Also, no homologues could be found in the LEE-encoded system of LcrV and LcrG, which are thought to regulate entry into the Ysc secretion apparatus from the cytoplasm [99, 100]. Similarly, we could not detect any LEE-encoded homologues of the needle-length molecular ruler YscP , even though, as we have reported elsewhere , PSI-BLAST searches do reveal sequence similarity at the C-terminus between YscP and the flagellar hook-length-determining protein FliK, implying that they employ similar mechanisms [102, 103]. Nor could we find any homologue of InvJ/Spa32, which, although showing no detectable sequence similarity to YscP or FliK, is thought to play a similar role to these proteins in needle-length determination in the Spi-1 and Mxi-Spa systems [67, 104].
Despite the frustrating lack of sequence similarities, two pieces of tantalising circumstantial evidence link FliK/YscP with InvJ/Spa32, and hint at the identity of an equivalent protein in the LEE-encoded system. Firstly, all four proteins are characterised by the presence of large amounts of random coil on secondary structure predictions (S. Lloyd, personal communication). Secondly, the genes for these proteins always occupy an equivalent position within the type-III secretion gene cluster: always next door but one to and downstream of the gene encoding the ATPase. When these criteria are applied to the LEE, an obvious candidate for needle-length determination emerges: Orf16 (28% random coil according to SOPMA ). Almost nothing is known about this gene/protein, which has no known homologues outside of the LEE. However, a mutant in this gene in Citrobacter is deficient in translocator secretion, but is still able to secrete effectors normally, and is severely attenuated in the mouse . These results are consistent with the hypothesis that Orf16 controls the switch between export of needle components and components of translocation apparatus. However, in the absence of structural or experimental data, it is premature to make an assertion of homology between Orf16 and YscP/FliK, particularly as tentative assignments tend to harden into dogma and then preclude consideration of other possibilities i.e. that different systems employ different mechanisms for the same purpose. Clearly, additional experimental data are needed to resolve this issue.
CesD is a member of the "chaperones of the translocators" family common to many T3SSs. We have already shown that this family is characterised by the presence of three tandem tetratricopeptide repeats . CesAB (previously Orf3) is a newly described chaperone that binds to EspA and EspB . Homology searches fail to reveal any homologues of this protein, even in the two other systems that possess obvious EspA homologues.
CesD2 (previously Orf27), is a recently described chaperone that, like CesD, binds to EspD . PSI-BLAST searches show that it is homologous to SseE from the Spi-2 system, CseE from the Cpi-2 system of Chromobacterium violaceum, LcrR from the Ysc-Yop system, AcrR from Aeromonas salmonicida and LssR from Photorhabdus luminescens. None of these homologues has been characterised aside from LcrR. An initial report suggested that a mutation in lcrR had a regulatory effect , although this was subsequently shown to be the result of a polar effect on the downstream gene lcrG . However, an lcrR mutant was detected in a signature-tagged mutagenesis screen of Y. pseudotuberculosis and shown to be attenuated both after oral and intra-peritoneal infection in Balb-C mice, even though the mutation did not have a measurable effect on Yop secretion or function in cell culture assays . These findings stand in contrast to a report that a Y. pseudotuberculosis lcrR mutant had no virulence phenotype after oral infection of Swiss albino mice . The homology between LcrR and CesD2 and between EspD and YopB suggests that LcrR is likely to bind to YopB (Table 1).
Ler is a regulatory protein encoded with the LEE1 operon, which has been extensively studied for its effects on gene expression within the LEE-encoded system [120–123]. It is known to be homologous to the global regulator H-NS, and is likely to adopt a similar fold, including a dimeric structure, particularly as key residues in the N-terminal dimerization domain are conserved in both proteins [124–126]. H-NS is known to form heterodimers with other proteins from the H-NS family [124, 127, 128] and to form complexes with members of the Hha family of regulators . As the attaching and effacing pathogens possess two other H-NS-like proteins besides Ler (H-NS and StpA), and possess Hha-like proteins (Hha, YdgT), it is tempting to ask whether Ler could, under any circumstances, form complexes with H-NS or StpA or with the Hha-like proteins (and if not, why not?).
Quorum sensing through the AI-2 pathway is known to influence type-III secretion in the LEE-encoded system [130–134]. In addition, there is some limited evidence to support an impact on type-III secretion of quorum-sensing through the AI-1/acyl-homoserine lactone route . However, this latter route is less plausible, as E. coli cannot produce these signalling molecules, although it can probably sense signals produced by other bacteria through the LuxR-like protein SdiA . It is thus surprising that domain searches with the protein sequence from rOrf1 of the LEE identify a PFAM domain (PF06977) entitled "SdiA-regulated", which represents a conserved region approximately 100 residues long common to YjiK from E. coli K-12 and several other bacterial outer-membrane proteins, including some Salmonella proteins that are regulated by SdiA  (data not shown). This hints at a link between SdiA-mediated sensing of other bacteria and LEE-mediated type-III secretion, which, twinned with growing evidence from Salmonella and Vibrio for an influence of SdiA/LuxR on virulence and type-III secretion [136, 138–141], suggests that it might be worth taking a fresh look at the influence of SdiA on LEE-mediated type-III secretion. Exhaustive PSI-BLAST searches suggest that the "SdiA-regulated domain" in fact consists of several NHL-like repeats (PF01436), which are likely to fold up into a six-bladed beta-propeller [142, 143] (data not shown).
GrlR and GlrA (previously known as Orf10/L0044 and Orf11 respectively) are a pair of recently characterised regulatory proteins encoded by adjacent genes within the LEE [33, 144]. Similarity has already been reported between GlrA and CaiF, a transcriptional regulator from E. coli and to a Salmonella protein . PSI-BLAST searches confirm this and report similarity to several other putative transcriptional regulators (e.g. the R721-plasmid-encoded regulator YheC from E. coli K-12). PSI-BLAST searches with GlrR identify only one homologue outside the LEE – a protein encoded within the unfinished Salmonella bongori genome (data not shown). Interestingly, the S. bongori glrR homologue is located next to a grlA homologue, suggesting a functional link between the two proteins and adding weight to the finding from yeast two-hybrid studies that GlrA and GlrR interact with each other .
The remaining coding sequences
Six coding sequences, which initially appeared to be orphans, have recently been shown to essential for LEE-encoded type-III secretion: Orf2, Orf4, Orf12, Orf15, Orf16 and Orf29 . Thus, something can now be said about all of them based on homology and/or experimental data. PSI-BLAST searches show that Orf2 has homologues in several other systems: ssaE in Spi-2, Cv2595 in Cpi-2 and yscE in the Ysc-Yop system (data not shown). Orf2 should thus be renamed EscE. The ssaE gene was found to be induced after invasion of a murine cultured cell line . Like YscE in Yersinia , Orf2/EscE is necessary for type-III secretion through the LEE-encoded T3SS . YscE interacts with TyeA  – thus it would seem likely that Orf2/EscE interacts with the C-terminal portion of SepL (Table 1). YscE also interacts with YscG, a protein with no detectable homologue in the LEE-encoded system .
Orf4, Orf12 and Orf29 possess homologues in T3SSs closely related to the LEE-encoded system, although not in the Ysc-Yop system (Table 1). None of the Orf4 or Orf29 homologues has been investigated. However, the homologue of Orf12 in the Spi-2 system, SsaM, has recently been characterised in S. enterica . An ssaM deletion mutant was attenuated in vivo – in vitro it over-secreted, but failed to translocate, the effector SseJ. Furthermore, it also failed to secrete translocators. In addition, SsaM was shown to interact with another effector SpiC within the bacterial cell. The story told by this complex set of phenotypes, reminiscent of the multi-faceted YopN-like proteins or of the FliK/YscP-like proteins, stands at odds with the simpler global defect in type-III secretion reported in an orf12 deletion mutant . As with the YopN-like and FliK/YscP-like proteins, it remains to be seen whether these apparently dissimilar functional properties of homologous proteins from different T3SSs will converge into a unified mechanistic picture or represent genuine evolutionary divergence of function.
Given that Orf29 is known to interact with Orf2/EscE , it is tempting to speculate that, even in absence of homology, Orf29 and the chief binding partner of YscE, YscG might play similar roles. As noted above, Orf16 is a candidate for needle-length control, while the function and evolutionary origin of Orf15 remain mysterious.
In this survey, we have discovered several new homologous relationships and woven together threads of evidence from our own in silico surveys and from published experimental studies to craft numerous novel functional predictions about the LEE-encoded and other T3SSs that can now be tested in the laboratory. We invite the scientific community to test our predictions. Furthermore, in this sequence-rich, post-genomic era [2, 23], we believe the time is right to roll out similar bioinformatics analyses over all known type-III secretion systems and invite others to join us in this enterprise on our new web site, 3Base, dedicated to type-III secretion http://3base.bham.ac.uk.
Peptide sequences of the 44 LEE-encoded proteins from the genome-sequenced enterohaemorrhagic E. coli O157:H7 Sakai strain (encoded by genes ECs4548-ECs4591 in the Sakai nomenclature) were extracted from the relevant GenBank file (accession number dbj|BA000007). Analyses were performed on a Macintosh Dual 2 GHz G5 running OSX10.3.
PSI-BLAST searches  were performed on LEE-encoded proteins against a composite database that included the NCBI's non-redundant (NR) protein database and a database of predicted protein sequences from unfinished genomes (VGE-PEPT: searchable on our web site http://www.vge.ac.uk). To explore all possibilities, multiple PSI-BLAST searches were run for the LEE-encoded proteins using different combinations of substitution matrices (BLOSUM45, BLOSUM62, BLOSUM80), low-complexity filtering (ON, OFF), compositional-based statistics (ON, OFF), E-value inclusion threshold (E = 0.002, E = 0.02) and database (VGE-PEPT + NR, VGE-PEPT alone). PSI-BLAST results were parsed using home-produced Perl scripts and any searches that uncovered significant or near-significant matches to other type III secretion associated proteins were flagged for closer examination and potential homologues retrieved and analysed further. In some cases, PSI-BLAST searches were supplemented with iterative Hmmer searches  and searches of the PFAM, CDD and SMART databases [40, 55, 152].
Most LEE proteins produced statistically significant matches to other Type III associated proteins of known and unknown function under PSI-BLAST default conditions (except for the absence of filtering and compositional based statistics) within 1 to 5 iterations. In some cases (e.g. ECs4584/ORF5), a significant PSI-BLAST hit was dependent on an absence of low-complexity eukaryote sequences in the target database, whereas in others (e.g. ECs4572/rORF8) significant hits were only identified using the BLOSUM80 matrix.
In most cases alignments were generated from homologues identified in PSI-BLAST searches with T-coffee under default settings . In the case of the EscD alignment, the FHA-like and BON-like regions were aligned separately using T-coffee. The former was then aligned to the FHA domain profile (SM00240) from the SMART database and the latter to the BON domain profile from PFAM (PF04972), respectively, using hmmalign from the Hmmer package. Similarly, the SepQ alignment was generated by aligning the COG1886 and Pfam:SpoA domain profiles with hmmalign. The EscI alignment was generated using T-coffee to align homologues parsed from the PSI-BLAST searches before the FliH sequence from E. coli K-12 was aligned to it using hmmalign. All multiple alignments were manually curated to ensure that secondary structure elements were not broken and to minimise the number of misaligned regions (as assessed using ClustalX and T-coffee [153, 154]). Alignments were coloured using default CHROMA settings .
We thank the MRC for funding SB's Bioinformatics Fellowship; we thank the Division of Infection and Immunity and the Medical School, University of Birmingham for funding CMB and the 3Base project. We thank the British Biotechnology and Biologivcal Sciences Research Council for funding other experimental and bioinformatics work within the Pallen group on E. coli and type-III secretion (grant references EGA16107, D13414, 02B1D08033). We thank an anonymous reviewer for pointing out the similar genetic locations of sepD and yscB.
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