Characterization of an autotransporter adhesin protein shared by Burkholderia mallei and Burkholderia pseudomallei
© Lafontaine et al.; licensee BioMed Central Ltd. 2014
Received: 31 December 2013
Accepted: 8 April 2014
Published: 14 April 2014
Autotransporters form a large family of outer membrane proteins specifying diverse biological traits of Gram-negative bacteria. In this study, we report the identification and characterization of a novel autotransporter gene product of Burkholderia mallei (locus tag BMA1027 in strain ATCC 23344).
Database searches identified the gene in at least seven B. mallei isolates and the encoded proteins were found to be 84% identical. Inactivation of the gene encoding the autotransporter in the genome of strain ATCC 23344 substantially reduces adherence to monolayers of HEp-2 laryngeal cells and A549 type II pneumocytes, as well as to cultures of normal human bronchial epithelium (NHBE). Consistent with these findings, expression of the autotransporter on the surface of recombinant E. coli bacteria increases adherence to these cell types by 5–7 fold. The gene specifying the autotransporter was identified in the genome of 29 B. pseudomallei isolates and disruption of the gene in strain DD503 reduced adherence to NHBE cultures by 61%. Unlike B. mallei, the mutation did not impair binding of B. pseudomallei to A549 or HEp-2 cells. Analysis of sera from mice infected via the aerosol route with B. mallei and B. pseudomallei revealed that animals inoculated with as few as 10 organisms produce antibodies against the autotransporter, therefore indicating expression in vivo.
Our data demonstrate that we have identified an autotransporter protein common to the pathogenic species B. mallei and B. pseudomallei which mediates adherence to respiratory epithelial cells and is expressed in vivo during the course of aerosol infection.
Autotransporter proteins are the largest known family of virulence factors expressed by Gram-negative bacteria and play prominent roles in processes such as invasion, serum resistance[2, 3], phospholipolysis[4–6], cytotoxicity, adherence[8, 9], survival within eukaryotic cells, intracellular motility, cell-to-cell aggregation[12, 13], and biofilm formation[14, 15]. These molecules display conserved structural features including an N-terminal surface-exposed domain responsible for the biological function and a hydrophobic C-terminus that tethers the autotransporter to the outer membrane (OM). Based on the structure of the C-terminus, autotransporters can be classified as conventional or oligomeric[16–21]. The C-terminus of conventional autotransporters consists of ~300 amino acids (aa) forming 10–12 antiparallel β-strands, while that of oligomeric autotransporters is substantially shorter (~70 aa) and specifies only 4 β-strands. Because of their structure and role in virulence, autotransporters are attractive targets for developing countermeasures against pathogenic organisms. Large portions of autotransporters are located on the bacterial surface and therefore readily accessible for recognition by the immune system. Additionally, autotransporters play important roles in pathogenesis, thus targeting them may hinder the ability to cause disease. This hypothesis is supported by several studies demonstrating the effectiveness of autotransporter-based countermeasures. For example, immunization with Neisseria meningitidis NadA elicits antibodies (Abs) binding to the bacterial surface and promoting complement-mediated killing[22, 23], which is key to protection against this organism. Antibodies against Haemophilus influenzae Hap block adherence to epithelial cells and immunization with Hap protects mice in nasopharyngeal colonization studies[24, 25]. Vaccination with the Proteus mirabilis autotransporter cytotoxin Pta yields Abs that not only reduce bacterial burden in a murine urinary tract infection model, but also neutralize the cytotoxic activity of Pta for bladder cells. Moreover, Bordetella pertussis Pertactin, an autotransporter adhesin, is a component of licensed vaccines for whooping cough (http://www.cdc.gov/vaccines/pubs/pinkbook/downloads/pert.pdf).
Burkholderia mallei and Burkholderia pseudomallei are closely related Gram-negative organisms for which developing efficacious countermeasures is highly desirable. Both species are classified as Tier 1 agents by the U.S. Federal Select Agent Program because of concerns regarding their use as bioweapons, especially since B. mallei has been utilized in this manner on more than one occasion[27–31]. Burkholderia mallei is a host-adapted pleomorphic coccobacillus that does not persist in the environment outside of its natural equine reservoir. The bacterium causes the highly contagious zoonotic disease glanders, which primarily affects horses, and is endemic to parts of Asia, Africa, South America and the Middle East[27, 32–38]. In humans, infection typically occurs via the cutaneous or aerosol route upon contact with infected animals. Clinical manifestations include fever, pneumonia, necrosis of the trachea and bronchi, bacteremia, and dissemination of B. mallei to organs where it causes necrotizing abscesses. Burkholderia pseudomallei is a saprophyte of wet soils and is endemic to countries bordering the equator. The organism can infect most mammals and causes the disease melioidosis in humans, a febrile illness that varies greatly in its clinical presentation. Disease states range from flu-like malaise to septicemia, chronic abscess formation in deep tissues, or bacteremic pneumonia[33, 39–45]. Infection is generally acquired by percutaneous inoculation, ingestion and inhalation of aerosols, and the risk of contracting disease is proportionate to the concentration of B. pseudomallei in soil. Burkholderia pseudomallei is a leading cause of sepsis and bacteremic pneumonia in Southeast Asia and Australia, and melioidosis is increasingly recognized as an emerging infectious diseases in many tropical regions of the world[40, 46, 47].
Glanders and melioidosis have high mortality rates (up to 50%) despite aggressive antimicrobial therapy. The recommended treatment involves the use of ceftazidime and meropenem (intensive phase) and TMP-SMX and co-amoxiclav (eradication phase) for several months. Response to treatment is slow and eradication of the agents is difficult, often resulting in protracted alternating bouts of remission and exacerbation. There are no vaccines available to protect against either Burkholderia species. Clearly, there is a need to identify and characterize targets for developing countermeasures for these organisms. The genomes of B. mallei and B. pseudomallei have been reported to encode multiple autotransporters[49–51]. In this study, we examined one of these gene products and evaluated it role in adherence in vitro and virulence in a mouse aerosol model of infection.
Identification of a gene encoding a potential autotransporter adhesin shared by B. mallei and B. pseudomallei
Characteristics a of BMA1027 orthologous genes and their encoded products
Predicted protein (aa)
Potential signal sequence cleavage sitec
The published genomic sequence of the B. pseudomallei strain K96243 was found to specify a BMA1027 ortholog (locus tag # BPSL1631, Figure 1B) that is 89% identical to that of B. mallei ATCC 23344. The BMA1027 ortholog was sequenced from the B. pseudomallei strain used in our laboratory, DD503, and was predicted to encode a protein that is 97% and 87% identical to that of B. pseudomallei K96243 and B. mallei ATCC 23344, respectively (Figure 1C). Database searches with the NCBI genomic BLAST service also identified orthologs in several B. pseudomallei and B. mallei isolates. Seven B. mallei and twenty-nine B. pseudomallei strains for which sequences are available through the service were found to have the gene. Characteristics of these ORFs are listed in the Additional files1 and2. Overall, the proteins are 87-100% identical and differ primarily in the number and/or arrangement of SLST repeats, YadA stalk domains, and/or NSTA elements in their passenger domains. Based on these results, we conclude that BMA1027 orthologs are well-conserved gene products shared by B. mallei and B. pseudomallei. While preparing this article, Campos and colleagues published a report in which they functionally characterized autotransporter genes specified by the B. pseudomallei strain 1026b. One of these molecules corresponds to the BMA1027 ortholog (locus tag # BP1026B_1575), which the authors designated bpaC. Henceforth, BMA1027 and orthologs will be referred to as BpaC.
Expression and functional properties of the BpaC protein in E. coli
Quantitative adherence assays revealed that E. coli expressing BpaC binds to HEp-2 (laryngeal) and A549 (lung) human epithelial cells at levels 7- and 5-fold greater than bacteria carrying pCC1.3, respectively (Figure 2C). BpaC expression was also found to increase adherence by 7-fold to normal human bronchial epithelium (NHBE) cultured in an air-liquid interface system, which has been shown to represent an environment similar to the airway lumen in vivo[54, 63, 64]. These results demonstrate that BpaC mediates adherence to respiratory epithelial cells. Burkholderia pseudomallei and B. mallei are facultative intracellular bacteria that replicate within several eukaryotic cell types. Moreover, autotransporter adhesins frequently perform additional functions including invasion, intracellular motility, and survival inside host cells. For these reasons, we examined the ability of E. coli expressing BpaC to invade epithelial cells and survive within murine macrophages. The results of these experiments indicated that BpaC does not substantially increase invasion of epithelial cells, phagocytosis of recombinant bacteria by J774A.1 murine macrophages, or survival inside these immune cells (data not shown).
In vitro characterization of B. mallei and B. pseudomallei mutant strains
To study the functional properties of the bpaC gene product in Burkholderia, we constructed isogenic bpaC mutants of B. pseudomallei DD503 and B. mallei ATCC 23344. Whole cell lysates and sarkosyl-insoluble OM proteins were prepared from these strains and analyzed by western blot to verify lack of BpaC expression in the mutants. However, α-BpaC Abs did not react with protein preparations of parent or mutant strains (data not shown). Other methods such as immunoprecipitation and immunofluorescence-labeling also failed to detect BpaC expression. These results indicate that the bpaC gene is expressed at very low levels under the laboratory growth conditions we used to propagate the organisms.
As stated earlier, autotransporter adhesins often perform multiple biological functions including invasion and survival within host cells. In addition, B. pseudomallei and B. mallei are facultative intracellular pathogens that effectively replicate inside professional phagocytic cells. Therefore, we measured the ability of Burholderia mutant and parent strains to invade epithelial cells (A549 and HEp-2) and replicate within J774A.1 murine macrophages. The results of these experiments indicated that the bpaC mutation does not cause a defect in invasion of epithelial cells, phagocytosis of recombinant bacteria by J774A.1 murine macrophages, or growth inside these cells (data not shown). The bpaC mutants did not show defects in resistance to the bactericidal activity of normal human serum (data not shown), which is another biological function commonly associated with Oca autotransporters[2, 3, 19, 65, 66].
Virulence of B. mallei and B. pseudomallei mutant strains and BpaC expression in vivo
Median lethal dose determination of B. mallei and B. pseudomallei WT and mutant strains
Inoculating dose (CFU)
B. mallei a
ATCC 23344 (WT)
B. mallei a
bpaC KO (mutant)
PBS (control) a
B. pseudomallei b
B. pseudomallei b
bpaC KO (mutant)
PBS (control) b
The genome of B. mallei ATCC 23344 has been reported to specify eight autotransporter gene products and of these, only BoaA (adhesin,) and BimA (intracellular motility protein,[11, 68]) have been functionally characterized. Both are classified as oligomeric autotransporters because they possess a short C-terminal transporter module predicted to form 4 β-strands, which anchor the molecules on the bacterial surface. In the present study, we characterized a third B. mallei ATCC 2334 oligomeric autotransporter, BpaC (BMA1027). Comparative sequence analyses indicate that the gene product is conserved among B. mallei isolates (see Additional files1 and2) and resembles members of the Oca (oligomeric coiled-coil adhesin) sub-family of oligomeric autotransporter proteins[16, 19–21]. Consistent with this, inactivation of bpaC in the genome of B. mallei ATCC 23344 reduces adherence to monolayers of A549 (lung) and HEp-2 (larynx) cells grown in submerged cultures (Figure 3D and E, respectively). Though these cells are relevant to aerosol infection by B. mallei, they lack key features of the airway mucosa such as cilia and mucociliary activity. Ciliated cells contribute to preventing colonization of the respiratory tract by pathogenic agents by moving secretions (and trapped organisms) toward the laryngopharynx for expectoration or swallowing to the stomach (where the acidic pH neutralizes organisms). For these reasons, we measured the adherence of the B. mallei bpaC mutant to cultures of NHBE grown in an air-liquid interface system. These cultures mimic the structure and function of the airway mucosa as they form a pseudostratified epithelium with tight junctions, contain ciliated and mucus-producing goblet cells, and display mucociliary activity[63, 64]. Quantitative assays using this system revealed that adherence of the bpaC mutant was reduced by 66% (Figure 3F).
Orthologs of BpaC were identified in 29 B. pseudomallei isolates (see Additional files1 and2). The genome of some of these strains has not been completed, resulting in the passenger domain and transporter module of BpaC seemingly specified by two different ORFs (e. g. B7210, 112, BPC006, 354e). Inactivation of bpaC in the genome of the B. pseudomallei strain DD503 caused a 2.6-fold reduction in adherence to NHBE cultures (Figure 3C), which is consistent with the phenotype of the B. mallei bpaC mutant (Figure 3F). However, the bpaC mutation did not affect adherence of B. pseudomallei to A549 or HEp-2 cells (Figure 3A and B, respectively). One possible explanation for this lack of effect is that other adhesins expressed by the B. pseudomallei DD503 bpaC mutant provide a high background of adherence to A549 and HEp-2 monolayers. For instance, BoaA and BoaB have been shown to mediate binding of B. pseudomallei DD503 to HEp-2 and A549 cells. Moreover, it was recently demonstrated that the B. pseudomallei gene products BpaA, BpaB, BpaD, BpaE and BpaF all play a role in adherence to A549 cells. The genes encoding these molecules are present in the genome of strain DD503.
While preparing this article, Campos and colleagues published a study in which they demonstrate that BpaC is an adhesin for A549 cells. The authors reported that a mutation in the bpaC gene of B. pseudomallei strain 340 causes an ~ 10-fold reduction in adherence. These results are in contrast with our data showing that a B. pseudomallei DD503 bpaC mutant binds to A549 cells at wild-type levels (Figure 3A). One possible explanation for this phenotypic difference is that we performed adherence assays using plate-grown bacteria, and infected A549 cells for 3 hours before washing off unbound B. pseudomallei and measuring cell-binding. Campos et al. used overnight broth cultures to inoculate A549 cells and infected monolayers for only 2 hours. The method used to construct mutants might have impacted the experimental outcome of adherence assays as well. In the present study, an internal portion of the bpaC ORF was replaced with a zeocin resistance marker and this mutation was introduced in the genome of B. pseudomallei DD503 via allelic exchange. In contrast, the bpaC gene of B. pseudomallei strain 340 was disrupted via co-integration of a large plasmid (~9-kb) in the genome. It is unlikely that the strain background in which the mutations were constructed has any effect on the phenotype of bpaC mutants since strains DD503 and 340 are both antibiotic-sensitive derivatives of the same clinical isolate, B. pseudomallei 1026b. Despite these differences, our data constitute independent proof of the role of BpaC as an adhesin. These results are substantiated by showing that expression of BpaC on the surface of recombinant E. coli bacteria increases adherence to NHBE, A549 and HEp-2 cells (Figure 2).
Given the phenotype of mutants in assays with NHBE cultures and that adherence is a key step in pathogenesis by most infectious agents, we expected the bpaC mutation to reduce the virulence of B. mallei and/or B. pseudomallei in a mouse model of aerosol infection. However, the results of our animal experiments indicate that the mutants are as virulent as wild-type strains (Table 2). Presumably, other adhesins expressed by the bpaC mutants provide sufficient adherence to the murine airway mucosa to allow colonization at wild-type levels and for the normal course of disease to ensue. It is unlikely that the lack of phenotype we observed in vivo is due to non-expression of BpaC. Though we discovered that B. pseudomallei DD503 and B. mallei ATCC 23344 do not produce detectable amounts of BpaC under routine laboratory growth conditions, ELISA with sera from mice that survived acute aerosol infection with the agents show that animals produce Abs against the protein (Figure 4A and B). Moreover, sera from horses with experimental glanders have been shown to contain high antibody titers against BpaC. These results are particularly significant as horses are the natural host and reservoir for B. mallei and arguably the most relevant surrogate to study glanders. Together, these data demonstrate that BpaC is expressed in vivo and elicits the production of Abs during infection.
The infection model we used to examine the effect of the bpaC mutation might have impacted the outcome of virulence experiments. This hypothesis is supported by the Campos et al. study in which they show that the bpaC mutation reduces the ability of B. pseudomallei strain 340 to disseminate and/or survive in the liver. In these experiments, BALB/c mice were infected intranasally with 500 CFU of the agent and bacterial loads in tissues were determined 48 hours post-infection. In contrast, we inoculated BALB/c mice intratracheally using a Microsprayer®, which nebulizes bacteria directly into the lungs, infected animals with doses ranging from 102 to 105 CFU, and determined bacterial burden in survivors 6–10 days post-infection (Table 2). It is also known that the choice of bacterial strains, inoculation route, and animal background can significantly affect the course of disease by B. pseudomallei and B. mallei. For example, the LD50 value of the same B. pseudomallei isolate has been shown to differ by several orders of magnitude in C57BL/6 mice and BALB/c mice. Therefore, a complete understanding of the role of BpaC in pathogenesis may require the use of multiple infection models.
This study demonstrates that BpaC is an autotransporter protein, which mediates adherence of B. mallei and B. pseudomallei to host cells that are relevant to pathogenesis by the organisms. We show that BpaC is conserved among isolates of both Burkholderia species, is expressed in vivo, and elicits production of Abs during infection. Hence, BpaC displays many properties of an important virulence factor and potential target for developing countermeasures. Though our animal experiments indicate that a mutation in bpaC does not impact the virulence of B. mallei or B. pseudomallei, adherence to host surfaces is a key early step in pathogenesis by most infectious agents. To accomplish this, pathogenic organisms typically express multiple adhesins to ascertain host colonization. It is likely that disruption of multiple genes specifying adherence factors, including bpaC, will result in decreased virulence and clarify the role of the autotransporter in the pathogenesis of B. mallei and B. pseudomallei. Continued investigation of BpaC will yield important information regarding the complex biology and virulence of these organisms, and may contribute to development of comprehensive countermeasures targeting autotransporters and their roles in pathogenesis.
Strains, plasmids, tissue culture cell lines and growth conditions
Strains and plasmids
Parental strain; polymixin B resistant, zeocin sensitive, kanamycin sensitive (derived from clinical isolate 1026b)
Isogenic bpaC mutant strain of DD503; polymixin B resistant, zeocin resistant, kanamycin sensitive
Wild-type strain; polymixin B resistant, zeocin sensitive, kanamycin sensitive
Isogenic bpaC mutant strain of ATCC 23344; polymixin B resistant, zeocin resistant, kanamycin sensitive
Expression strain for purification of His-tagged BpaC
Strain used for conjugational transfer of pKASbpaC.zeo to B. pseudomallei and B. mallei
Cloning vector, chloramphenicol resistant
pCC1 containing the B. pseudomallei DD503 bpaC gene, chloramphenicol resistant
pCCbpaC in which a zeocin resistance cassette was introduced near the middle of the bpaC ORF; chloramphenicol and zeocin resistant
pCC1-based plasmid control, does not confer adherence to human epithelial cells; chloramphenicol resistant
Mobilizable suicide plasmid; kanamycin resistant
pKAS46 containing the insert from pCCbpaC.zeo
Source of the zeocin resistance marker
Plasmid expressing aa 392–1068 of B. pseudomallei 1026b BpaC fused to six N-terminal histidine residues, introduced in E. coli TUNER and used to purify His-tagged BpaC protein for antibody production and ELISA experiments; chloramphenicol resistant.
Escherichia coli was cultured at 37°C using LSLB supplemented with 15 μg/mL chloramphenicol, 50 μg/mL kanamycin, or 50 μg/mL zeocin, where indicated. For conjugation experiments, LSLB was supplemented with 10 mM MgSO4. For assays with E. coli clones carrying pCC1-based plasmids, the CopyControl™ Induction Solution (epicentre® Illumina®) was added to LSLB as previously reported.
The cell lines HEp-2 (human laryngeal epithelium; ATCC CCL-23), A549 (type II alveolar epithelium; ATCC CCL85) and J774A.1 (murine macrophages; ATCC TIB-67) were cultured as outlined by others[5, 55]. Normal human bronchial epithelium (NHBE; LONZA) was expanded, cryopreserved and cultured in an air-liquid interface system as previously described[54, 63, 64]. The apical surface of the NHBE was exposed to air for a minimum of 3 weeks prior to use in adherence assays to ascertain proper cellular differentiation and the development of functional cilia.
Recombinant DNA methodology
Standard molecular biology techniques were performed as described elsewhere. Genomic DNA was purified from Burkholderia using the Easy-DNA™ Kit (Life Technologies™). Plasmid DNA was isolated with the QIAprep Spin Miniprep kit (QIAGEN). The Platinum® Pfx DNA Polymerase (Life Technologies™) was used to amplify the 3.8-kb bpaC gene of B. pseudomallei DD503 with primers P1 (5’-ATA CCC AAA TCG GCG TTC TCT GGT-3′) and P2 (5′-TGC GCG AAT CAA TCG AGA TAC CCA-3′) and the PCR product was used as a template in sequencing reactions. The amplicon was also cloned in the vector pCC1™ using the CopyControl™ PCR cloning kit (epicentre® Illumina®), producing the plasmid pCCbpaC (Table 3). The latter was sequenced to determine that PCR did not introduce mutations resulting in aa substitutions in the bpaC gene product.
Construction of isogenic mutant strains of B. mallei and B. pseudomallei
The plasmid pCCbpaC was digested with the enzyme Nsi I (New England BioLabs®, Inc.) to remove a 0.6-kb fragment internal to the bpaC ORF, treated with the End-It™ DNA End Repair Kit (epicentre® Illumina®), and ligated with a 0.4-kb zeocin resistance cassette to yield the construct pCCbpaC.zeo. This plasmid was restricted with Bam HI (New England BioLabs®, Inc.) and a 3.4-kb fragment corresponding to the bpaC ORF disrupted by the insertion of the zeocin resistance cassette was excised from an agarose gel, purified with the High Pure PCR Product Purification kit (Roche Applied Science), and treated with the End-It™ DNA End Repair Kit. This blunt DNA fragment was then cloned in the suicide vector pKAS46. The resulting plasmid, designated pKASbpaC.zeo, was introduced in the E. coli strain S17 by electroporation, and subsequently transferred into B. mallei ATCC 23344 or B. pseudomallei DD503 by conjugation, as previously reported[55, 80]. Upon conjugation, Burkholderia colonies were selected for resistance to zeocin. These putative mutants were then screened by PCR using Platinum® Pfx DNA Polymerase with primers P1 and P2. The primers yielded a PCR product of 3.8-kb in the parent strains and a smaller amplicon of 3.6-kb in bpaC mutants. The PCR products from mutant strains were sequenced to verify proper allelic exchange and successful disruption of bpaC.
Nucleotide sequence and bioinformatic analyses
PCR products and plasmids were sequenced at the University of Michigan Sequencing Core (http://seqcore.brcf.med.umich.edu). Chromatograms were assembled using the Sequencher® 5 software (Gene Codes Corporation). Sequence analyses were performed using Vector NTI (Life Technologies™) and the various online tools available through the EsPASy Bioinformatics Resource Portal (http://www.expasy.org). Signal sequence cleavage sites were determined using the SignalP 4.1 server (http://www.cbs.dtu.dk/services/SignalP). The B. mallei ATCC 23344 bpaC gene product (locus tag # BMA1027) was identified by searching the genome of the organism for the presence of a YadA anchor domain (Pfam database number PF3895.10) through the NCBI genomic BLAST service using the blastp program (http://www.ncbi.nlm.nih.gov/sutils/genom_table.cgi). The other bpaC gene products described in this study were identified using the predicted aa sequence of the B. mallei ATCC 23344 BpaC protein to search the genomes of the B. mallei and B. pseudomallei strains available through the NCBI genomic BLAST service utilizing the tblastn and blastp programs. Structural features of the BpaC proteins (helical regions, hydrophobic β-strands) were identified with the PSIPRED Protein Sequence Analysis Workbench service (http://bioinf.cs.ucl.ac.uk/psipred/).
Experiments with epithelial cells and J774 murine macrophages
Adherence, invasion, and intracellular survival assays were performed as previously reported by our laboratory[53–55]. Cells were inoculated with bacteria at a multiplicity of infection (MOI) of 100. Duplicate assays were performed on at least 3 occasions. Statistical analyses were performed using the Mann–Whitney test (GraphPad Prism® 6 software) and P values < 0.05 are reported as statistically significant.
The method used to examine the effect of bpaC mutations on the ability of Burkholderia to resist the bactericidal activity of complement is outlined elsewhere[9, 77, 81]. We used final concentrations of 50% and 25% serum in assays with B. pseudomallei and B. mallei, respectively.
Protein preparations, western blot, purification of recombinant BpaC protein, and antibody production
Sarkosyl-insoluble OM protein preparations were obtained as described by Carlone et al.. The methods used to prepare whole cell lysates and perform western blot experiments are described elsewhere[8, 53, 54, 57, 83, 84]. His-tagged recombinant BpaC was obtained from cultures of E. coli TUNER carrying the plasmid pELHisBPSL1631-BMA1027, as previously outlined by our laboratory. To obtain polyclonal Abs directed against BpaC, the purified His-tagged protein was emulsified in Freund’s adjuvants (SIGMA-ALDRICH®) and used to immunize female BALB/c mice as reported by Lafontaine and colleagues.
Immunofluorescence labeling of E. coli and microscopy
Expression of BpaC on the surface of E. coli recombinant bacteria was visualized by immunofluorescence microscopy as outlined by Balder et al.. Briefly, paraformaldehyde-fixed E. coli cells were spotted onto glass slides. These bacteria were probed with α-BpaC polyclonal Abs, followed by incubation with a goat α-mouse antibody labeled with Alexa Fluor 546® (Life Technologies™) and the nucleic acid dye DAPI (Life Technologies™). Slides were examined by microscopy using a Zeiss LSM 510 Meta confocal system.
Duplicate wells of Immulon™ 2HB plates (Thermo Scientific Nunc) were coated overnight at 4C° with 1 μg of His-tagged BpaC. Excess unbound antigen was removed by washing the wells with PBS + 0.05% Tween 20 (PBST), and the wells were then blocked with PBS + 0.05% containing 3% dry milk (blocking buffer) for 1 hour at room temperature. After washing with PBST, the wells were probed overnight at 4°C with sera from mice that survived acute aerosol infection with B. mallei ATCC 23344 and B. pseudomallei 1026b diluted in blocking buffer. After this incubation, the wells were washed with PBST and incubated overnight with a goat α-mouse antibody conjugated to Horse Radish Peroxidase (SouthernBiotech) diluted in blocking buffer. After washing off the excess secondary antibody with PBST, 100 μL of the SureBlue™ TMB Microwell Peroxidase Substrate (KPL) was added to the wells. Color development, which is indicative of Abs binding to BpaC, was measured spectrophotometrically by determining the absorbance of well contents at a wavelength of 650 nm.
Female BALB/c mice (6–8 weeks of age) were purchased from Frederick National Laboratory for Cancer Research. The animals were anesthetized by injecting a dose of 250 mg/kg of 2,2,2 tribromoethanol (SIGMA-ALDRICH®) intraperitoneally. Once anesthetized, mice were inoculated intratracheally with 50 μL of bacterial suspensions using a Microsprayer® model I-1C (PennCentury™) as previously reported by our laboratory. Infected animals were monitored twice daily. Humane end-points were strictly observed. Mice exhibiting signs of moderate to severe discomfort were euthanized. This was accomplished by anesthetizing the animals with 2,2,2 tribromoethanol followed by cervical dislocation, in accordance with the AVMA Guidelines on euthanasia. Food and water were provided ad libitum. Analgesics were not used as they may have affected the experimental outcomes of the studies. Survival data were analyzed using the Kaplan-Meier method and the LD50 values were calculated according to Reed and Muench.
Compliance and animal research ethic statements
All experiments with live B. pseudomallei and B. mallei were performed inside a Class II Biosafety Cabinet in a BSL3 laboratory and in compliance with the rules and regulations of the U.S. Federal Select Agent Program. The experiments were approved by the University of Georgia’s Institutional Biosafety Committee (IBC).
Animal experiments were carried out in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. The experiments were approved by the University of Georgia’s Institutional Animal Care and Use Committee (IACUC). All efforts were made to minimize animal suffering.
Oligomeric coiled-coil adhesin
Median lethal dose
Low salt luria bertani
Colony forming units
Open reading frame
Normal human bronchial epithelium.
The study was supported by NIAID award AI062775 to ERL and by institutional funds from the College of Veterinary Medicine at the University of Georgia (UGA) to ERL and RJH. We thank Donald Woods (University of Calgary) for providing strains. We thank Laura Wiese (UGA), Sean Buskirk (UGA), Lauren Snipes (UGA), Xiudan Gao (UGA) and Serena Lipski (University of Toledo) for technical assistance. We thank Shawn Zimmerman (UGA) and Tomislav Jelesijevic (UGA) for their assistance redacting the manuscript.
- Capecchi B, Adu-Bobie J, Di Marcello F, Ciucchi L, Masignani V, Taddei A, Rappuoli R, Pizza M, Arico B: Neisseria meningitidis NadA is a new invasin which promotes bacterial adhesion to and penetration into human epithelial cells. Mol Microbiol. 2005, 55 (3): 687-698.View ArticlePubMedGoogle Scholar
- Roggenkamp A, Ackermann N, Jacobi CA, Truelzsch K, Hoffmann H, Heesemann J: Molecular analysis of transport and oligomerization of the Yersinia enterocolitica adhesin YadA. J Bacteriol. 2003, 185 (13): 3735-3744. 10.1128/JB.185.13.3735-3744.2003.PubMed CentralView ArticlePubMedGoogle Scholar
- Attia AS, Lafontaine ER, Latimer JL, Aebi C, Syrogiannopoulos GA, Hansen EJ: The UspA2 protein of Moraxella catarrhalis is directly involved in the expression of serum resistance. Infect Immun. 2005, 73 (4): 2400-2410. 10.1128/IAI.73.4.2400-2410.2005.PubMed CentralView ArticlePubMedGoogle Scholar
- Farn JL, Strugnell RA, Hoyne PA, Michalski WP, Tennent JM: Molecular characterization of a secreted enzyme with phospholipase B activity from Moraxella bovis. J Bacteriol. 2001, 183 (22): 6717-6720. 10.1128/JB.183.22.6717-6720.2001.PubMed CentralView ArticlePubMedGoogle Scholar
- Lipski SL, Akimana C, Timpe JM, Wooten RM, Lafontaine ER: The Moraxella catarrhalis autotransporter McaP is a conserved surface protein that mediates adherence to human epithelial cells through its N-terminal passenger domain. Infect Immun. 2007, 75 (1): 314-324. 10.1128/IAI.01330-06.PubMed CentralView ArticlePubMedGoogle Scholar
- Timpe JM, Holm MM, Vanlerberg SL, Basrur V, Lafontaine ER: Identification of a Moraxella catarrhalis outer membrane protein exhibiting both adhesin and lipolytic activities. Infect Immun. 2003, 71 (8): 4341-4350. 10.1128/IAI.71.8.4341-4350.2003.PubMed CentralView ArticlePubMedGoogle Scholar
- Maroncle NM, Sivick KE, Brady R, Stokes FE, Mobley HL: Protease activity, secretion, cell entry, cytotoxicity, and cellular targets of secreted autotransporter toxin of uropathogenic Escherichia coli. Infect Immun. 2006, 74 (11): 6124-6134. 10.1128/IAI.01086-06.PubMed CentralView ArticlePubMedGoogle Scholar
- Bullard B, Lipski S, Lafontaine ER: Regions important for the adhesin activity of Moraxella catarrhalis Hag. BMC Microbiol. 2007, 7: 65-10.1186/1471-2180-7-65.PubMed CentralView ArticlePubMedGoogle Scholar
- Lipski SL, Holm MM, Lafontaine ER: Identification of a Moraxella catarrhalis gene that confers adherence to various human epithelial cell lines in vitro. FEMS Microbiol Lett. 2007, 267 (2): 207-213. 10.1111/j.1574-6968.2006.00549.x.View ArticlePubMedGoogle Scholar
- Fexby S, Bjarnsholt T, Jensen PO, Roos V, Hoiby N, Givskov M, Klemm P: Biological Trojan horse: antigen 43 provides specific bacterial uptake and survival in human neutrophils. Infect Immun. 2007, 75 (1): 30-34. 10.1128/IAI.01117-06.PubMed CentralView ArticlePubMedGoogle Scholar
- Stevens JM, Ulrich RL, Taylor LA, Wood MW, Deshazer D, Stevens MP, Galyov EE: Actin-binding proteins from Burkholderia mallei and Burkholderia thailandensis can functionally compensate for the actin-based motility defect of a Burkholderia pseudomallei bimA mutant. J Bacteriol. 2005, 187 (22): 7857-7862. 10.1128/JB.187.22.7857-7862.2005.PubMed CentralView ArticlePubMedGoogle Scholar
- Klemm P, Hjerrild L, Gjermansen M, Schembri MA: Structure-function analysis of the self-recognizing Antigen 43 autotransporter protein from Escherichia coli. Mol Microbiol. 2004, 51 (1): 283-296.View ArticlePubMedGoogle Scholar
- Heras B, Totsika M, Peters KM, Paxman JJ, Gee CL, Jarrott RJ, Perugini MA, Whitten AE, Schembri MA: The antigen 43 structure reveals a molecular Velcro-like mechanism of autotransporter-mediated bacterial clumping. Proc Natl Acad Sci U S A. 2014, 111 (1): 457-462. 10.1073/pnas.1311592111.PubMed CentralView ArticlePubMedGoogle Scholar
- Valle J, Mabbett AN, Ulett GC, Toledo-Arana A, Wecker K, Totsika M, Schembri MA, Ghigo JM, Beloin C: UpaG, a new member of the trimeric autotransporter family of adhesins in uropathogenic Escherichia coli. J Bacteriol. 2008, 190 (12): 4147-4167. 10.1128/JB.00122-08.PubMed CentralView ArticlePubMedGoogle Scholar
- Sherlock O, Schembri MA, Reisner A, Klemm P: Novel roles for the AIDA adhesin from diarrheagenic Escherichia coli: cell aggregation and biofilm formation. J Bacteriol. 2004, 186 (23): 8058-8065. 10.1128/JB.186.23.8058-8065.2004.PubMed CentralView ArticlePubMedGoogle Scholar
- Cotter SE, Surana NK, 3rd St Geme JW: Trimeric autotransporters: a distinct subfamily of autotransporter proteins. Trends Microbiol. 2005, 13 (5): 199-205. 10.1016/j.tim.2005.03.004.View ArticlePubMedGoogle Scholar
- Henderson IR, Navarro-Garcia F, Nataro JP: The great escape: structure and function of the autotransporter proteins. Trends Microbiol. 1998, 6 (9): 370-378. 10.1016/S0966-842X(98)01318-3.View ArticlePubMedGoogle Scholar
- Stathopoulos C, Hendrixson DR, Thanassi DG, Hultgren SJ, 3rd St Geme JW, 3rd Curtiss R: Secretion of virulence determinants by the general secretory pathway in gram-negative pathogens: an evolving story. Microbes Infect. 2000, 2 (9): 1061-1072. 10.1016/S1286-4579(00)01260-0.View ArticlePubMedGoogle Scholar
- Henderson IR, Navarro-Garcia F, Desvaux M, Fernandez RC, Ala’Aldeen D: Type V protein secretion pathway: the autotransporter story. Microbiol Mol Biol Rev. 2004, 68 (4): 692-744. 10.1128/MMBR.68.4.692-744.2004.PubMed CentralView ArticlePubMedGoogle Scholar
- Linke D, Riess T, Autenrieth IB, Lupas A, Kempf VA: Trimeric autotransporter adhesins: variable structure, common function. Trends Microbiol. 2006, 14 (6): 264-270. 10.1016/j.tim.2006.04.005.View ArticlePubMedGoogle Scholar
- Hoiczyk E, Roggenkamp A, Reichenbecher M, Lupas A, Heesemann J: Structure and sequence analysis of Yersinia YadA and Moraxella UspAs reveal a novel class of adhesins. Embo J. 2000, 19 (22): 5989-5999. 10.1093/emboj/19.22.5989.PubMed CentralView ArticlePubMedGoogle Scholar
- Ciabattini A, Giomarelli B, Parigi R, Chiavolini D, Pettini E, Arico B, Giuliani MM, Santini L, Medaglini D, Pozzi G: Intranasal immunization of mice with recombinant Streptococcus gordonii expressing NadA of Neisseria meningitidis induces systemic bactericidal antibodies and local IgA. Vaccine. 2008, 26 (33): 4244-4250. 10.1016/j.vaccine.2008.05.049.View ArticlePubMedGoogle Scholar
- Bowe F, Lavelle EC, McNeela EA, Hale C, Clare S, Arico B, Giuliani MM, Rae A, Huett A, Rappuoli R, Dougan G, Mills KH: Mucosal vaccination against serogroup B meningococci: induction of bactericidal antibodies and cellular immunity following intranasal immunization with NadA of Neisseria meningitidis and mutants of Escherichia coli heat-labile enterotoxin. Infect Immun. 2004, 72 (7): 4052-4060. 10.1128/IAI.72.7.4052-4060.2004.PubMed CentralView ArticlePubMedGoogle Scholar
- Liu DF, Mason KW, Mastri M, Pazirandeh M, Cutter D, Fink DL, 3rd St Geme JW, Zhu D, Green BA: The C-terminal fragment of the internal 110-kilodalton passenger domain of the Hap protein of nontypeable Haemophilus influenzae is a potential vaccine candidate. Infect Immun. 2004, 72 (12): 6961-6968. 10.1128/IAI.72.12.6961-6968.2004.PubMed CentralView ArticlePubMedGoogle Scholar
- Cutter D, Mason KW, Howell AP, Fink DL, Green BA, 3rd St Geme JW: Immunization with Haemophilus influenzae Hap adhesin protects against nasopharyngeal colonization in experimental mice. J Infect Dis. 2002, 186 (8): 1115-1121. 10.1086/344233.View ArticlePubMedGoogle Scholar
- Alamuri P, Eaton KA, Himpsl SD, Smith SN, Mobley HL: Vaccination with proteus toxic agglutinin, a hemolysin-independent cytotoxin in vivo, protects against Proteus mirabilis urinary tract infection. Infect Immun. 2009, 77 (2): 632-641. 10.1128/IAI.01050-08.PubMed CentralView ArticlePubMedGoogle Scholar
- Waag DM, Deshazer D: Glanders: New Insights into an old Disease. Biological Weapons Defense: Infectious Diseases and Counterbioterrorism. Edited by: Lindler LE LF, Korch GW. 2004, Totowa, New Jersey: Humana Press IncGoogle Scholar
- Alibek K, Handelman S: Biohazard: The Chilling True Story of the Largest Covert Biological Weapons Programin the World. 1999, New York: Random HouseGoogle Scholar
- Lehavi O, Aizenstien O, Katz LH, Hourvitz A: [Glanders--a potential disease for biological warfare in humans and animals]. Harefuah. 2002, 141: 119-Spec No:88–91Google Scholar
- Wheelis M: First shots fired in biological warfare. Nature. 1998, 395 (6699): 213-View ArticlePubMedGoogle Scholar
- Wheelis M: Biological Sabotage in World War I. Biological and Toxin Weapons: Research, Development, and use from the Middle Ages to 1945. Edited by: Geissler EMoon JEC. 1999, Oxford: Oxford University Press, 35-72.Google Scholar
- Bondi SK, Goldberg JB: Strategies toward vaccines against Burkholderia mallei and Burkholderia pseudomallei. Expert Rev Vaccines. 2008, 7 (9): 1357-1365. 10.1586/14760522.214.171.1247.PubMed CentralView ArticlePubMedGoogle Scholar
- Galyov EE, Brett PJ, Deshazer D: Molecular insights into Burkholderia pseudomallei and Burkholderia mallei pathogenesis. Annu Rev Microbiol. 2010, 64: 495-517. 10.1146/annurev.micro.112408.134030.View ArticlePubMedGoogle Scholar
- Arun S, Neubauer H, Gurel A, Ayyildiz G, Kuscu B, Yesildere T, Meyer H, Hermanns W: Equine glanders in Turkey. Vet Rec. 1999, 144 (10): 255-258. 10.1136/vr.144.10.255.View ArticlePubMedGoogle Scholar
- Neubauer H, Meyer H, Finke EJ: Human glanders. Revue Internationale des Services de Sante des Forces Armees. 1997, 70: 258-265.Google Scholar
- Whitlock GC, Estes DM, Torres AG: Glanders: off to the races with Burkholderia mallei. FEMS Microbiol Lett. 2007, 277 (2): 115-122. 10.1111/j.1574-6968.2007.00949.x.View ArticlePubMedGoogle Scholar
- Srinivasan A, Kraus CN, DeShazer D, Becker PM, Dick JD, Spacek L, Bartlett JG, Byrne WR, Thomas DL: Glanders in a military research microbiologist. N Engl J Med. 2001, 345 (4): 256-258. 10.1056/NEJM200107263450404.View ArticlePubMedGoogle Scholar
- Gregory BC, Waag DM: Medical Aspects of Biological Warfare. 2007, Washington DC: Borden Institute Walter Reed Army Medical Center, 121-146. Glanders, U.S Army Medical Department Borden Insitute Textbooks of Biological WarfareGoogle Scholar
- Cheng AC, Currie BJ: Melioidosis: epidemiology, pathophysiology, and management. Clin Microbiol Rev. 2005, 18 (2): 383-416. 10.1128/CMR.18.2.383-416.2005.PubMed CentralView ArticlePubMedGoogle Scholar
- Wiersinga WJ, Currie BJ, Peacock SJ: Melioidosis. N Engl J Med. 2012, 367 (11): 1035-1044. 10.1056/NEJMra1204699.View ArticlePubMedGoogle Scholar
- Wiersinga WJ, van der Poll T, White NJ, Day NP, Peacock SJ: Melioidosis: insights into the pathogenicity of Burkholderia pseudomallei. Nat Rev Microbiol. 2006, 4 (4): 272-282. 10.1038/nrmicro1385.View ArticlePubMedGoogle Scholar
- Currie BJ, Fisher DA, Howard DM, Burrow JN, Lo D, Selva-Nayagam S, Anstey NM, Huffam SE, Snelling PL, Marks PJ Stephens DP, Lum GD, Jacups SP, Krause VL: Endemic melioidosis in tropical northern Australia: a 10-year prospective study and review of the literature. Clin Infect Dis. 2000, 31 (4): 981-986. 10.1086/318116.View ArticlePubMedGoogle Scholar
- Currie BJ, Fisher DA, Anstey NM, Jacups SP: Melioidosis: acute and chronic disease, relapse and re-activation. Trans R Soc Trop Med Hyg. 2000, 94 (3): 301-304. 10.1016/S0035-9203(00)90333-X.View ArticlePubMedGoogle Scholar
- Adler NR, Govan B, Cullinane M, Harper M, Adler B, Boyce JD: The molecular and cellular basis of pathogenesis in melioidosis: how does Burkholderia pseudomallei cause disease?. FEMS Microbiol Rev. 2009, 33 (6): 1079-1099. 10.1111/j.1574-6976.2009.00189.x.View ArticlePubMedGoogle Scholar
- Vietri NJ, Deshazer D: Medical Aspects of Biological Warfare. 2007, Washington DC: Borden Institute Walter Reed Army Medical Center, 147-166. Melioidosis, U.S Army Medical Department Borden Insitute Textbooks of Biological WarfareGoogle Scholar
- Dance DA: Melioidosis as an emerging global problem. Acta Trop. 2000, 74 (2–3): 115-119.View ArticlePubMedGoogle Scholar
- Rolim DB, Vilar DC, Sousa AQ, Miralles IS, de Oliveira DC, Harnett G, O’Reilly L, Howard K, Sampson I, Inglis TJ: Melioidosis, northeastern Brazil. Emerg Infect Dis. 2005, 11 (9): 1458-1460. 10.3201/eid1109.050493.PubMed CentralView ArticlePubMedGoogle Scholar
- Lipsitz R, Garges S, Aurigemma R, Baccam P, Blaney DD, Cheng AC, Currie BJ, Dance DA, Gee JE, Larsen J, Limmathurotsakul D, Morrow MG, Norton R, O’Mare E, Peacock SJ, Pesik N, Rogers LP, Schweizer HP, Steinmetz I, Tan G, Tan P, Wiersinga WJ, Wuthiekanun V, Smith TL: Workshop on Treatment of and Postexposure Prophylaxis for Burkholderia pseudomallei and B. mallei infection, 2010. Emerg Infect Dis. 2012, 18 (12): online reportGoogle Scholar
- Lazar Adler NR, Stevens JM, Stevens MP, Galyov EE: Autotransporters and their role in the virulence of Burkholderia pseudomallei and Burkholderia mallei. Front Microbiol. 2011, 2: 151-PubMedGoogle Scholar
- Campos CG, Borst L, Cotter PA: Characterization of BcaA, a putative classical autotransporter protein in Burkholderia pseudomallei. Infect Immun. 2013, 81 (4): 1121-1128. 10.1128/IAI.01453-12.PubMed CentralView ArticlePubMedGoogle Scholar
- Campos CG, Byrd MS, Cotter PA: Functional characterization of Burkholderia pseudomallei trimeric autotransporters. Infect Immun. 2013, 81 (8): 2788-2799. 10.1128/IAI.00526-13.PubMed CentralView ArticlePubMedGoogle Scholar
- Nummelin H, Merckel MC, Leo JC, Lankinen H, Skurnik M, Goldman A: The Yersinia adhesin YadA collagen-binding domain structure is a novel left-handed parallel beta-roll. Embo J. 2004, 23 (4): 701-711. 10.1038/sj.emboj.7600100.PubMed CentralView ArticlePubMedGoogle Scholar
- Bullard B, Lipski SL, Lafontaine ER: Hag directly mediates the adherence of Moraxella catarrhalis to human middle ear cells. Infect Immun. 2005, 73 (8): 5127-5136. 10.1128/IAI.73.8.5127-5136.2005.PubMed CentralView ArticlePubMedGoogle Scholar
- Balder R, Krunkosky TM, Nguyen CQ, Feezel L, Lafontaine ER: Hag mediates adherence of Moraxella catarrhalis to ciliated human airway cells. Infect Immun. 2009, 77 (10): 4597-4608. 10.1128/IAI.00212-09.PubMed CentralView ArticlePubMedGoogle Scholar
- Balder R, Lipski S, Lazarus JJ, Grose W, Wooten RM, Hogan RJ, Woods DE, Lafontaine ER: Identification of Burkholderia mallei and Burkholderia pseudomallei adhesins for human respiratory epithelial cells. BMC Microbiol. 2010, 10: 250-10.1186/1471-2180-10-250.PubMed CentralView ArticlePubMedGoogle Scholar
- Lazar Adler NR, Dean RE, Saint RJ, Stevens MP, Prior JL, Atkins TP, Galyov EE: Identification of a Predicted Trimeric Autotransporter Adhesin Required for Biofilm Formation of Burkholderia pseudomallei. PLoS One. 2013, 8 (11): e79461-10.1371/journal.pone.0079461.PubMed CentralView ArticlePubMedGoogle Scholar
- Cope LD, Lafontaine ER, Slaughter CA, Hasemann CA, Aebi C, Henderson FW, McCracken GH, Hansen EJ: Characterization of the Moraxella catarrhalis uspA1 and uspA2 genes and their encoded products. J Bacteriol. 1999, 181 (13): 4026-4034.PubMed CentralPubMedGoogle Scholar
- Brooks MJ, Sedillo JL, Wagner N, Laurence CA, Wang W, Attia AS, Hansen EJ, Gray-Owen SD: Modular arrangement of allelic variants explains the divergence in Moraxella catarrhalis UspA protein function. Infect Immun. 2008, 76 (11): 5330-5340. 10.1128/IAI.00573-08.PubMed CentralView ArticlePubMedGoogle Scholar
- Brooks MJ, Sedillo JL, Wagner N, Wang W, Attia AS, Wong H, Laurence CA, Hansen EJ, Gray-Owen SD: Moraxella catarrhalis binding to host cellular receptors is mediated by sequence-specific determinants not conserved among all UspA1 protein variants. Infect Immun. 2008, 76 (11): 5322-5329. 10.1128/IAI.00572-08.PubMed CentralView ArticlePubMedGoogle Scholar
- Lafontaine ER, Cope LD, Aebi C, Latimer JL, McCracken GH, Hansen EJ: The UspA1 protein and a second type of UspA2 protein mediate adherence of Moraxella catarrhalis to human epithelial cells in vitro. J Bacteriol. 2000, 182 (5): 1364-1373. 10.1128/JB.182.5.1364-1373.2000.PubMed CentralView ArticlePubMedGoogle Scholar
- Moore RA, DeShazer D, Reckseidler S, Weissman A, Woods DE: Efflux-mediated aminoglycoside and macrolide resistance in Burkholderia pseudomallei. Antimicrob Agents Chemother. 1999, 43 (3): 465-470.PubMed CentralPubMedGoogle Scholar
- Balder R, Hassel J, Lipski S, Lafontaine ER: Moraxella catarrhalis strain O35E expresses two filamentous hemagglutinin-like proteins that mediate adherence to human epithelial cells. Infect Immun. 2007, 75 (6): 2765-2775. 10.1128/IAI.00079-07.PubMed CentralView ArticlePubMedGoogle Scholar
- Krunkosky TM, Fischer BM, Martin LD, Jones N, Akley NJ, Adler KB: Effects of TNF-alpha on expression of ICAM-1 in human airway epithelial cells in vitro. Signaling pathways controlling surface and gene expression. Am J Respir Cell Mol Biol. 2000, 22 (6): 685-692. 10.1165/ajrcmb.22.6.3925.View ArticlePubMedGoogle Scholar
- Krunkosky TM, Jordan JL, Chambers E, Krause DC: Mycoplasma pneumoniae host-pathogen studies in an air-liquid culture of differentiated human airway epithelial cells. Microb Pathog. 2007, 42 (2–3): 98-103.View ArticlePubMedGoogle Scholar
- Pearson MM, Hansen EJ: Identification of gene products involved in Biofilm production by Moraxella catarrhalis ETSU-9 in vitro. Infect Immun. 2007, 75 (9): 4316-4325. 10.1128/IAI.01347-06.PubMed CentralView ArticlePubMedGoogle Scholar
- Wang W, Pearson MM, Attia AS, Blick RJ, Hansen EJ: A UspA2H-negative variant of Moraxella catarrhalis strain O46E has a deletion in a homopolymeric nucleotide repeat common to uspA2H genes. Infect Immun. 2007, 75 (4): 2035-2045. 10.1128/IAI.00609-06.PubMed CentralView ArticlePubMedGoogle Scholar
- Lafontaine ER, Zimmerman S, Shaffer TL, Michel F, Gao X, Hogan RJ: Use of a safe, reproducible and rapid aerosol delivery method to study infection by Burkholderia pseudomallei and Burkholderia mallei in mice. PLoS One. 2013, 8 (10): e76804-10.1371/journal.pone.0076804.PubMed CentralView ArticlePubMedGoogle Scholar
- Stevens MP, Stevens JM, Jeng RL, Taylor LA, Wood MW, Hawes P, Monaghan P, Welch MD, Galyov EE: Identification of a bacterial factor required for actin-based motility of Burkholderia pseudomallei. Mol Microbiol. 2005, 56 (1): 40-53. 10.1111/j.1365-2958.2004.04528.x.View ArticlePubMedGoogle Scholar
- Mima T, Schweizer HP: The BpeAB-OprB efflux pump of Burkholderia pseudomallei 1026b does not play a role in quorum sensing, virulence factor production, or extrusion of aminoglycosides but is a broad-spectrum drug efflux system. Antimicrob Agents Chemother. 2010, 54 (8): 3113-3120. 10.1128/AAC.01803-09.PubMed CentralView ArticlePubMedGoogle Scholar
- Tiyawisutsri R, Holden MT, Tumapa S, Rengpipat S, Clarke SR, Foster SJ, Nierman WC, Day NP, Peacock SJ: Burkholderia Hep_Hap autotransporter (BuHA) proteins elicit a strong antibody response during experimental glanders but not human melioidosis. BMC Microbiol. 2007, 7: 19-10.1186/1471-2180-7-19.PubMed CentralView ArticlePubMedGoogle Scholar
- Goodyear A, Bielefeldt-Ohmann H, Schweizer H, Dow S: Persistent gastric colonization with Burkholderia pseudomallei and dissemination from the gastrointestinal tract following mucosal inoculation of mice. PLoS One. 2012, 7 (5): e37324-10.1371/journal.pone.0037324.PubMed CentralView ArticlePubMedGoogle Scholar
- Revelli DA, Boylan JA, Gherardini FC: A non-invasive intratracheal inoculation method for the study of pulmonary melioidosis. Front Cell Infect Microbiol. 2012, 2: 164-PubMed CentralView ArticlePubMedGoogle Scholar
- Hoppe I, Brenneke B, Rohde M, Kreft A, Haussler S, Reganzerowski A, Steinmetz I: Characterization of a murine model of melioidosis: comparison of different strains of mice. Infect Immun. 1999, 67 (6): 2891-2900.PubMed CentralPubMedGoogle Scholar
- Leakey AK, Ulett GC, Hirst RG: BALB/c and C57Bl/6 mice infected with virulent Burkholderia pseudomallei provide contrasting animal models for the acute and chronic forms of human melioidosis. Microb Pathog. 1998, 24 (5): 269-275. 10.1006/mpat.1997.0179.View ArticlePubMedGoogle Scholar
- Nierman WC, DeShazer D, Kim HS, Tettelin H, Nelson KE, Feldblyum T, Ulrich RL, Ronning CM, Brinkac LM, Daugherty SC, Davidsen TD, Deboy RT, Dimitrov G, Dodson RJ, Durkin AS, Gwinn ML, Haft DH, Khouri H, Kolonay JF, Madupu R, Mohammoud Y, Nelson WC, Radune D, Romero CM, Sarria S, Selengut J, Shamblin C, Sullivan SA, White O, Yu Y: Structural flexibility in the Burkholderia mallei genome. Proc Natl Acad Sci U S A. 2004, 101 (39): 14246-14251. 10.1073/pnas.0403306101.PubMed CentralView ArticlePubMedGoogle Scholar
- Simon R, Priefer U, Puhler A: A broad host range mobilisation system for in vivo genetic engineering: transposon mutagenesis in gram-negative bacteria. Bio/Technology. 1983, 1: 784-791. 10.1038/nbt1183-784.View ArticleGoogle Scholar
- Holm MM, Vanlerberg SL, Foley IM, Sledjeski DD, Lafontaine ER: The Moraxella catarrhalis porin-like outer membrane protein CD is an adhesin for human lung cells. Infect Immun. 2004, 72 (4): 1906-1913. 10.1128/IAI.72.4.1906-1913.2004.PubMed CentralView ArticlePubMedGoogle Scholar
- Skorupski K, Taylor RK: Positive selection vectors for allelic exchange. Gene. 1996, 169 (1): 47-52. 10.1016/0378-1119(95)00793-8.View ArticlePubMedGoogle Scholar
- Sambrook J, Russell DW: Molecular Cloning: A Laboratory Manual (Third Edition). 2001, Woodbury NY: Cold Spring Harbor Laboratory Press, 3Google Scholar
- Burtnick M, Bolton A, Brett P, Watanabe D, Woods D: Identification of the acid phosphatase (acpA) gene homologues in pathogenic and non-pathogenic Burkholderia spp. facilitates TnphoA mutagenesis. Microbiology. 2001, 147 (Pt 1): 111-120.View ArticlePubMedGoogle Scholar
- Aebi C, Lafontaine ER, Cope LD, Latimer JL, Lumbley SL, McCracken GH, Hansen EJ: Phenotypic effect of isogenic uspA1 and uspA2 mutations on Moraxella catarrhalis 035E. Infect Immun. 1998, 66 (7): 3113-3119.PubMed CentralPubMedGoogle Scholar
- Carlone GM, Thomas ML, Rumschlag HS, Sottnek FO: Rapid microprocedure for isolating detergent-insoluble outer membrane proteins from Haemophilus species. J Clin Microbiol. 1986, 24 (3): 330-332.PubMed CentralPubMedGoogle Scholar
- Shaffer TL, Balder R, Buskirk SW, Hogan RJ, Lafontaine ER: Use of the Chinchilla model to evaluate the vaccinogenic potential of the Moraxella catarrhalis filamentous hemagglutinin-like proteins MhaB1 and MhaB2. PLoS One. 2013, 8 (7): e67881-10.1371/journal.pone.0067881.PubMed CentralView ArticlePubMedGoogle Scholar
- Patrick CC, Kimura A, Jackson MA, Hermanstorfer L, Hood A, McCracken GH, Hansen EJ: Antigenic characterization of the oligosaccharide portion of the lipooligosaccharide of nontypable Haemophilus influenzae. Infect Immun. 1987, 55 (12): 2902-2911.PubMed CentralPubMedGoogle Scholar
- Lafontaine ER, Wagner NJ, Hansen EJ: Expression of the Moraxella catarrhalis UspA1 protein undergoes phase variation and is regulated at the transcriptional level. J Bacteriol. 2001, 183 (5): 1540-1551. 10.1128/JB.183.5.1540-1551.2001.PubMed CentralView ArticlePubMedGoogle Scholar
- Reed LJ, Muench H: A simple method for estimating fifty percent end points. Am J Hyg. 1938, 27: 793-497.Google Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.