Human serum inhibits adhesion and biofilm formation in Candida albicans
© Ding et al.; licensee BioMed Central Ltd. 2014
Received: 12 October 2013
Accepted: 21 March 2014
Published: 28 March 2014
Candida albicans can form biofilms on intravenous catheters; this process plays a key role in the pathogenesis of catheter infections. This study evaluated the effect of human serum (HS) on C. albicans biofilm formation and the expression of adhesion-related genes in vitro. A C. albicans laboratory strain (ATCC90028) and three clinical strains were grown for 24 h in RPMI 1640 supplemented with HS or RPMI 1640 alone (as a control). The growth of biofilm cells of four strains was monitored by a Live Cell Movie Analyzer, and by XTT reduction assay. The expression of the adhesion-related genes BCR1, ALS1, ALS3, HWP1 and ECE1 was analyzed by RT-PCR at three time points (60 min, 90 min, and 24 h).
In the adhesion phase, C. albicans cells kept a Brownian movement in RPMI medium containing HS until a large number of germ tubes were formed. In the control group, C. albicans cells quickly adhered to the bottom of the reaction plate. Compared with RPMI 1640, medium supplemented with 3–50% HS caused a significant decrease in biofilm development (all p < 0.001). However, the presence of HS had no significant inhibitory effect on the pre-adhered biofilms (all p > 0.05). Biofilm formation was also inhibited by heat-inactivated and proteinase K pre-treated HS. The presence of 50% HS did not significantly affect the planktonic growth of C. albicans (p > 0.05). At three time points, HS inhibited expression of the ALS1 and ALS3 genes and promoted expression of the HWP1 and ECE1 genes. Significant up-regulation of BCR1 was observed only at the 90-min point.
Human serum reduces biofilm formation by inhibiting the adhesion of C. albicans cells. This response may be associated with the down-regulation of adhesion-related genes ALS1, ALS3 and BCR1. The inhibitory serum component is protease-resistant and heat stable.
KeywordsHuman serum Biofilm Adhesion Candida albicans
Candida spp. are the fourth most common cause of nosocomial bloodstream infections , and Candida albicans accounts for approximately 50% of cases of candidemia . Frequently, candidemia is associated with C. albicans colonization of indwelling devices, such as catheters, endotracheal tubes, and pacemakers [3–6]. In fact, C. albicans is the most common fungus in biofilms formed on medical devices . Biofilm formation is a complex, multicellular process, consisting of cell adhesion, growth, morphogenic switching between yeast and filamentous states, and quorum sensing [8, 9]. Adhesion of C. albicans cells to materials or host cells is a prerequisite for biofilm formation, and cell-cell interactions may be important in the hierarchical organization of cells within the biofilm . Moreover, biofilm formation of C. albicans is governed by a tightly woven gene network composed of six transcription regulators and their target genes . The zinc finger transcription factor BCR1 and its target genes, ALS1, ALS3, HWP1, and ECE1, play an important role, especially in the process of adhesion [11–13].
Human serum (HS) is a complex medium composed of proteins, lipids, and small molecules. The interaction of C. albicans with serum has been of long-standing interest in the field of fungal pathogenesis. Because Candida spp. can form biofilms on intravenous catheters and other inserted medical devices that may come into contact with blood, serum is regarded as an external cue to trigger biofilm formation. Yuthika et al. reported that 3% human serum can promote the formation of C. albicans biofilms. However, other researches revealed that serum can inhibit biofilm formation in some bacteria. Another study showed that human serum and fetal bovine serum (FBS) inhibit biofilm formation in Staphylococcus aureus, and Hammond et al. found that adult bovine serum (ABS) or adult human serum (AHS) also inhibits P. aeruginosa biofilm formation on plastic surfaces, including intravenous catheters. Some studies revealed the ability of serum components to prevent the formation of bacterial biofilms. It was reported that bovine serum albumin (BSA) caused a significant decrease in biofilm development . Abraham et al. indicated that a low molecular weight component of human serum inhibits biofilm formation in Staphylococcus aureus. In addition, one component of innate immunity also prevents bacterial biofilm development .
Therefore, our hypothesis is that the positive effect of human serum on Candida albicans biofilm formation may be due to many factors, so it is necessary to study the related molecular mechanism.
The C. albicansadhesion process
To directly observe the adhesion process of C. albicans, the Live Cell Movie Analyzer was used. For the first 2 or 3 h of biofilm formation, we took photos once per minute by means of continuous photographic techniques. When those pictures were played back in rapid succession, we got dynamic images of biofilm growth. Movie 1 shows that cells of C. albicans quickly adhered to the surface of polypropylene microtiter plates, formed germ tubes, and gradually extended in RPMI 1640 without HS (Additional file 1: Movie 1). However, in the RPMI 1640 with 50% HS, the cells of the same strain kept a Brownian motion at the beginning and could not quickly clung to the bottom of the plate. The Brownian motion lasted as long as about 2 h. The motion did not stop until the formation of a large number of germ tubes (Additional file 1: Movie 2). In the next hour (120–180 min), almost no C. albicans cells kept a Brownian motion, but the hyphae grew longer (Additional file 1: Movie 3). Movie 3 further shows that Brownian motion stops after 2 h (Additional file 1: Movie 1, Movie 2, and Movie 3).
Effect of human serum on germ tube formation of C. albicans
C. albicans cells were cultured in RPMI 1640 with and without 50% HS, and germ tube formation was continuously observed at 30, 60, 90, 120, and 180-min time points by Live Cell Movie Analyzer. For the first 90 min of culture, the germ tube formation rate of C. albicans cells in the experimental group (RPMI 1640 containing 50% human serum) was significantly lower than that in the control group. Over 2 h of incubation, there was no significant difference in the rate of germ tube formation between the two groups. With the further extension of incubation time (from 2 h to 3 h), the amount of hyphae gradually increased in the experimental group, just as in the control group (Additional file 2).
Effect of human serum on C. albicansbiofilms
Data comparing biofilm growth of C. albicans strains in the absence or presence of different concentrations of HS were obtained using a XTT reduction assay. Initially, the tests were performed using cells of strain ATCC90028 in RPMI 1640 containing different concentrations of HS (3%, 5%, 10%, and 50%).
To confirm the hypothesis that this effect was not specific to strain ATCC90028, we tested three unrelated clinical strains and found that HS also had the same effect on all three clinical strains (data not shown).
Characterization of the inhibitory components
Effect of human serum on planktonic growth of C. albicans
Effect of human serum on expression of adhesion-related genes
To make the transition from a commensal organism to a systemic pathogen, C. albicans must first enter the bloodstream. It can do so by taking advantage of medical devices, such as intravenous catheters, to enter the bloodstream directly, or it can cross intact or damaged gastrointestinal mucosa and enter into the bloodstream [5, 18]. In the bloodstream, C. albicans is exposed to the innate immune defenses. As a part of human innate immune system, serum and its components show different degrees of protection against systemic candidiasis.
In this study, the natural proliferation condition of C. albicans was monitored continuously by a Live Cell Movie Analyzer. C. albicans cells in HS moved with Brownian motion in the initial stage of culture, then failed adhere to the surface of polystyrene plates. This indicates that C. albicans may remain in a suspended status at the early period of entering the blood stream. Previous studies showed that free-flowing C. albicans can be rapidly cleared from the blood . We determined that human serum facilitates the removal of C. albicans by inhibiting the adhesion of C. albicans on the surface of the endothelial cells. C. albicans possesses virulence factors that are needed to establish candidiasis that are involved in the many steps of this complicated process, such as adhesion, phenotypic switching, morphogenesis, and biofilm formation . Some factors in the bloodstream, such as temperature and serum, facilitate the filamentation of C. albicans[21–23]. It is reported that filamentation is favorable for C. albicans adhesion and biofilm formation [24, 25]. However, filamentation failed to offset the biofilm formation inhibition caused by the HS-induced adhesion defect, as demonstrated in our study.
We also investigated the effect of serum on germ tube formation in C. albicans. Our results showed that the rate of germ tube formation is high in HS medium, but compared with the control group, germ tube formation in the experimental group was delayed in the initial stage of culture (within 90 min). This may be one of the reasons for HS-induced adhesion inhibition. Based on these results, we also think that RPMI 1640 medium may be a more suitable medium than human serum to conduct germ tube testing in C. albicans.
In the initial stage of biofilm culture (the adhesion period), low serum concentrations suppressed C. albicans biofilm formation. However, the serum had no effect on pre-adhered biofilms (90 min), even if the serum was in a very high concentration. Thus, we concluded that serum may inhibit biofilm formation by preventing the adhesion of C. albicans, in consensus with previous studies [15, 16]. Recent studies showed that addition of as little as 3% human serum to media can promote C. albicans biofilm formation , contrary to our results. This may be explained by the use of different materials, such as serum, culture medium, strains, adhesive medium, and so on.
It has been reported that IgG, LL-37, transferrin and lactoferrin, at concentrations close to those found in vivo, can reduce the capacity of C. albicans and bacteria to adhere to polystyrene [17, 26–28]. Thus, we initially hypothesized that some protein or antimicrobial peptide in human serum may inhibit the adhesion of C. albicans, and as a consequence, reduce biofilm formation. However, our results suggest that the compound in serum that inhibits C. albicans biofilm formation is not proteinaceous. Abraham et al. found that a low molecular weight component of human serum inhibits biofilm formation in Staphylococcus aureus, and the component was protease-resistant and heat stable. We conclude here that human serum may also contain non-protein component(s) that can inhibit the adhesion and biofilm formation of fungi and bacteria. To confirm this hypothesis, future studies are needed to identify this component of human serum.
In this study, planktonic growth of C. albicans was not inhibited by human serum, indicating that inhibition of biofilm formation was not due solely to growth inhibition.
Biofilm formation of C. albicans, a process that depends upon both cell-cell and cell-substrate adherence, is controlled by a tightly woven network of genes . Among this gene network, BCR1 is one of the best-characterized biofilm regulators [11–13, 29]. Through its adhesin targets ALS1, ALS3, HWP1 and ECE1, BCR1 mediates cell-substrate and cell-cell interactions in biofilms [30, 31]. In this study, at the adhesion stage of biofilm formation (60 min, 90 min), the expression of BCR1 went from less than to significantly higher than that of the control group. This may be due to the promoting effect of serum on hypha growth, as BCR1 RNA accumulation depends on the hyphal developmental activator TEC1. ALS1 and ALS3 are members of the agglutinin-like sequence (ALS) gene family that encodes cell-wall glycoproteins . Most Als proteins have adhesin functions [34, 35]. Mutational analysis indicates that strains lacking all functional ALS1 and ALS3 alleles (als1Δ/als1Δ als3Δ/als3Δ) failed to produce any detectable adherent cells in biofilm models both in vivo and in vitro, or in actual biofilm formation. The als1Δ/als1Δ mutants produced substantial biofilms, but the biofilms often sloughed off the substrate, while the als3Δ/als3Δ mutant only produced scant, disorganized biofilms on catheter material in vitro. Our data on transcript analysis showed that the expression of ALS1 and ALS3 were reduced at different time points in the biofilm adhesion stage. Therefore, we supposed that the anti-adhesion effect of human serum might occur via inhibition of the expression of ALS1 and ALS3, and therefore affect biofilm formation. Previous studies have shown that a bcr1Δ/ bcr1Δ mutant, which has reduced expression of ALS1, ALS3, and other adhesins, has defective biofilm formation in both an in vitro and in vivo catheter model . In this study, at 90 min of growth, the change in the levels of BCR1 level was different from ALS1 and ALS3, indicating that ALS1 and ALS3 are also affected by other factors [8, 36].
Interestingly, human serum promotes the expression of HWP1 and ECE1. HWP1 is a well-characterized hypha-specific gene that can mediate C. albicans cell-cell interactions and improve biofilm formation [37, 38]. Nobile et al. found that the expression of Hwp1 in Saccharomyces cerevisiae permits adherence to wild-type C. albicans but not an als1Δ/als1Δ als3Δ/als3Δ double mutant. In addition, a TDH3-HWP1 hybrid gene could not promote biofilm formation in the als1Δ/als1Δ als3Δ/als3Δ background in vitro or in vivo. Our study revealed that human serum decreased the expression level of ALS1 and ALS3, so overexpression of HWP1 failed to save the adhesion and biofilm formation of C. albicans. ECE1 was regarded as a hyphal-induced gene, although its mechanism of action is uncertain. Our study showed that hyphae were significantly greater in the presence of serum than in the control group, especially in the mature biofilm stage (data not shown). This may be due to the increase of ECE1 and HWP1.
In this study, we also tested the expression of adhesion-related genes in biofilms grown for 24 h and found that the expression trend of related genes at this time was similar to the adhesion phase, both in the reduction of ALS1 and ALS3 and the up-regulation of HWP1 and ECE1. The expression of the BCR1 gene, however, was significantly inhibited. Its level was far lower than that of the control group. All in all, the serum reduces BCR1 gene expression, and that might be a reason for biofilm inhibition.
In summary, our study demonstrated that human serum may reduce the biofilm formation of C. albicans by inhibiting adhesion. This inhibition is partly due to the down-regulation of adhesion-related genes, including ALS1, ALS3 and BCR1. Meanwhile, the inhibitory effect of human serum is caused by non-protein components in the serum. Therefore, biofilm formation in vivo may be “selected for” (possibly by immune pressure and sheer forces) rather than “induced” by serum at the level of transcription.
This study was approved by the Medical Ethics Committee of Beijing Friendship Hospital, Capital Medical University, Beijing, China (approval #BJFH-EC/2013-014), and individual informed consent was waived.
Four Candida albicans strains (laboratory strain ATCC90028 and three clinical isolates of C. albicans: 9079, y2991, 31448) were tested in this study. The three C. albicans bloodstream isolates were collected from three different intensive care patients admitted to the Beijing Friendship Hospital and were confirmed according to standard mycological methods, such as the germ tube test in serum, growth on CHROMagar Candida medium, and API testing methods. All isolates were stored in skim milk at -80°C until use.
Medium and growth conditions
Prior to each experiment, C. albicans strains were subcultured on Sabouraud's Agar (SDA) at 35°C for 24 h. To prepare the yeast inocula for biofilm growth, a loopful of the SDA culture was transferred into 25 ml of liquid yeast extract-peptone-dextrose (YPD) medium (1% yeast extract, 2% peptone, 2% glucose) and incubated at 30°C for 18 h in an orbital shaker (75 rpm). Then, the cells were harvested by centrifugation, washed twice in PBS (pH 7.2), re-suspended in RPMI 1640 medium (buffered to a pH of 7.0 with 0.165 M morpholinepropanesulfonic acid), and counted after serial dilution by a hemocytometer.
Human serum (HS) was pooled from healthy blood donors, and heat-inactivated serum was prepared by heating at 56°C for 30 min. Proteinase K-treated serum was prepared by incubating with 50 mg/mL proteinase K at 58°C for 1 h followed by incubation at 85°C for 1 h to inactivate the protease. All fractions were filter-sterilized (0.22-mm pore size filter).
Fungal biofilms were prepared as described on commercially available, pre-sterilized, flat-bottomed 96-well polystyrene microtiter plates (Corning) . Briefly, a cell suspension of 1.0 × 106 cells/ml was prepared in RPMI 1640 and RPMI 1640 + 50%, 10%, 5% or 3% HS. From those suspensions, 100 μl was introduced into wells and incubated at 37°C for 24 h without agitation, which allowed the cells to attach to the surface of the plate and form the biofilm structure.
To investigate the effect of HS on pre-adhered biofilms, C. albicans biofilms were prepared for 90 min (the adhesion phase) at 37°C as described above. The wells were washed twice with PBS to remove loosely adherent cells. Then, fresh RPMI 1640 (100 μl), containing different concentrations (3–50%) of HS were added and the plate was further incubated for 24 h at 37°C. RPMI 1640 medium without HS was included in control wells. The metabolic activity of the C. albicans biofilms was determined quantitatively using XTT reduction assay.
Dynamic monitoring of the adhesion process
Standard cell suspension of C. albicans was prepared in RPMI1640 or RPMI1640 containing different concentrations (3% to 50%) of HS, and 100 μl of those suspensions was introduced into 96-well polystyrene microtiter plates. After standing for 3 min, the plates were placed on Live Cell Movie Analyzer (JuLI™ Br., NanoEnTek Inc., Seoul, Korea) and incubated at 37°C. The instrument was set to continuous photographing mode with exposure 5%, brightness 13%, zoom level 4, interval 1 min, and total time 2 h (the experimental group was prolonged to 3 h). When it was finished, a total of 121 or 181 photos were obtained for the control and experimental groups, respectively. Then, those pictures were played back in rapid succession to observe the dynamic changes of the fungal cells (playing at a speed of 10 frames/s).
Quantitation of biofilms
At the end of the incubation, the supernatant was aspirated and the wells washed twice with PBS. The quantitation of biofilms was determined using 2,3-bis (2-methoxy-4-nitro-5-sulfo-phenyl)-2H-tetrazolium-5-carboxanilide (XTT) reduction assay that measures the activity of mitochondrial dehydrogenase . XTT solution (1 mg/ml) was prepared by dissolving XTT powder (Sigma, Shanghai, China) in PBS, and the solution was filter-sterilized (0.22-mm pore size filter). XTT solution (40 μl) was mixed with freshly prepared menadione solution (0.4 mM; 2 μl) (Sigma, Shanghai, China) at 20:1 (v/v) immediately prior to the assay. Thereafter, PBS (158 μl) was mixed with XTT-menadione solution (42 μl), transferred to each well containing pre-washed biofilms, and incubated in the dark for 3 h at 37°C. After the incubation, the colored supernatant (100 μl) was transferred to new microtiter plates, and the optical density of the supernatant was measured at 490 nm with a microplate reader (BIO-RAD, CA, USA) and imaged by a flatbed scanner (EPSON PERFECTION V700 PHOTO, Beijing, China). All assays were carried out in at least three replicates on different days.
Effect of human serum on planktonic growth of C. albicans
A cell suspension of 105 cells/ml was prepared in RPMI 1640, RPMI 1640 + 50% fresh HS, 50% heat-inactivated HS and 50% proteinase K-treated HS. At predetermined time points (0, 2, 4, 6, 12 and 24 h after incubation with agitation at 30°C), 100 μl aliquot was removed from every solution and serially diluted 10-fold in sterile water. A 100 μl aliquot from each dilution was streaked on the Sabouraud dextrose agar plate. Colony counts were determined after incubation at 30°C for 48 h. Three independent experiments were performed. Effect of human serum on growth of C. albicans was determined by analyzing the time-growth curve.
RT-PCR analysis of C. albicansadhesion-related genes
Quantitative real-time reverse transcription PCR (RT-PCR) was used to compare mRNA abundances of the genes of interest. A standard cell suspension of C. albicans (1 ml) was transferred into the wells of a pre-sterilized, flat-bottomed 24-well polystyrene microtiter plate (Corning, NY, USA). After incubation for 60 min, 90 min or 24 h at 37°C with or without HS, the supernatant was aspirated and the wells were washed twice with PBS. Total RNA was extracted from C. albicans biofilms using FastPure™ RNA kit (TaKaRa Biotechnology Co. Ltd, Dalian, China), according to the manufacturer’s manual. RNA concentrations and RNA purity were determined using a BioPhotometer spectrophotometer (Eppendorf, Germany). An equal amount of RNA was subjected to cDNA synthesis using the PrimeScript RT reagent kit (TaKaRa Biotechnology Co. Ltd, Dalian, China).
Primers used for RT-PCR experiments
Data were described as mean ± SD. All statistical analyses were performed by statistical analysis computer software package SPSS 17.0 (SPSS Inc., IL, USA). Student’s t-test or one-way ANOVA were used to compare the biofilm formation, planktonic growth, and the gene expression of C. albicans strains in the presence or absence of HS. Results with a p-value less than 0.05 were considered statistically significant.
This study was supported in part by the National Natural Science Foundation of China [grant number 30972819]. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
- Wisplinghoff H, Bischoff T, Tallent SM, Seifert H, Wenzel RP, Edmond MB: Nosocomial bloodstream infections in US hospitals: analysis of 24,179 cases from a prospective nationwide surveillance study. Clin Infect Dis. 2004, 39: 309-317. 10.1086/421946.View ArticlePubMed
- Hajjeh RA, Sofair AN, Harrison LH, Lyon GM, Arthington-Skaggs BA, Mirza SA, Phelan M, Morgan J, Lee-Yang W, Ciblak MA, Benjamin LE, Sanza LT, Huie S, Yeo SF, Brandt ME, Warnock DW: Incidence of bloodstream infections due to Candida species and in vitro susceptibilities of isolates collected from 1998 to 2000 in a population-based active surveillance program. J Clin Microbiol. 2004, 42: 1519-1527. 10.1128/JCM.42.4.1519-1527.2004.PubMed CentralView ArticlePubMed
- Kumamoto CA: Candida biofilms. Curr Opin Microbiol. 2002, 5: 608-611. 10.1016/S1369-5274(02)00371-5.View ArticlePubMed
- Douglas LJ: Candida biofilms and their role in infection. Trends Microbiol. 2003, 11: 30-36. 10.1016/S0966-842X(02)00002-1.View ArticlePubMed
- Kojic EM, Darouiche RO: Candida infections of medical devices. Clin Microbiol Rev. 2004, 17: 255-267. 10.1128/CMR.17.2.255-267.2004.PubMed CentralView ArticlePubMed
- Ramage G, Saville SP, Thomas DP, López-Ribot JL: Candida biofilms: an update. Eukaryot Cell. 2005, 4: 633-638. 10.1128/EC.4.4.633-638.2005.PubMed CentralView ArticlePubMed
- López-Ribot JL: Candida albicans biofilms: more than filamentation. Curr Biol. 2005, 15: R453-R455. 10.1016/j.cub.2005.06.020.View ArticlePubMed
- Li F, Svarovsky MJ, Karlsson AJ, Wagner JP, Marchillo K, Oshel P, Andes D, Palecek SP: Eap1p, an Adhesin That Mediates Candida albicans Biofilm Formation in Vitro and in Vivo. Eukaryot Cell. 2007, 6: 931-939. 10.1128/EC.00049-07.PubMed CentralView ArticlePubMed
- Deveau A, Hogan DA: Linking quorum sensing regulation and biofilm formation by Candida albicans. Methods Mol Biol. 2011, 692: 219-233. 10.1007/978-1-60761-971-0_16.View ArticlePubMed
- Nobile CJ, Fox EP, Nett JE, Sorrells TR, Mitrovich QM, Hernday AD, Tuch BB, Andes DR, Johnson AD: A recently evolved transcriptional network controls biofilm development in Candida albicans. Cell. 2012, 148: 126-138. 10.1016/j.cell.2011.10.048.PubMed CentralView ArticlePubMed
- Nobile CJ, Mitchell AP: Genetics and genomics of Candida albicans biofilm formation. Cell Microbiol. 2006, 8: 1382-1391. 10.1111/j.1462-5822.2006.00761.x.View ArticlePubMed
- Nobile CJ, Andes DR, Nett JE, Smith FJ, Yue F, Phan QT, Edwards JE, Filler SG, Mitchell AP: Critical Role of Bcr1-Dependent Adhesins in C. albicans Biofilm Formation In Vitro and In Vivo. PLoS Pathog. 2006, 2: 636-649.View Article
- Ganguly S, Mitchell AP: Mucosal biofilms of Candida albicans. Curr Opin Microbiol. 2011, 14: 380-385. 10.1016/j.mib.2011.06.001.PubMed CentralView ArticlePubMed
- Samaranayake YH, Cheung BP, Yau JY, Yeung SK, Samaranayake LP: Human Serum Promotes Candida albicans Biofilm Growth and Virulence Gene Expression on Silicone Biomaterial. PLoS One. 2013, 8: e62902-10.1371/journal.pone.0062902.PubMed CentralView ArticlePubMed
- Abraham NM, Jefferson KK: A low molecular weight component of serum inhibits biofilm formation in Staphylococcus aureus. Microb Pathog. 2010, 49: 388-391. 10.1016/j.micpath.2010.07.005.PubMed CentralView ArticlePubMed
- Hammond A, Dertien J, Colmer-Hamood JA, Griswold JA, Hamood AN: Serum inhibits P. aeruginosa biofilm formation on plastic surfaces and intravenous catheters. J Surg Res. 2010, 159: 735-746. 10.1016/j.jss.2008.09.003.View ArticlePubMed
- Singh PK, Parsek MR, Greenberg EP, Welsh MJ: A component of innate immunity prevents bacterial biofilm development. Nature. 2002, 417: 552-555. 10.1038/417552a.View ArticlePubMed
- Cole GT, Halawa AA, Anaissie EJ: The role of the gastrointestinal tract in hematogenous candidiasis: from the laboratory to the bedside. Clin Infect Dis. 1996, 22 (Suppl 2): 73-88.View Article
- Rogers T, Balish E: Experimental Candida albicans infection in conventional mice and germfree rats. Infect Immun. 1976, 14: 33-38.PubMed CentralPubMed
- Calderone RA, Fonzi WA: Virulence factors of Candida albicans. Trends Microbiol. 2001, 9: 327-335. 10.1016/S0966-842X(01)02094-7.View ArticlePubMed
- Biswas S, Van Dijck P, Datta A: Environmental sensing and signal transduction pathways regulating morphopathogenic determinants of Candida albicans. Microbiol Mol Biol Rev. 2007, 71: 348-376. 10.1128/MMBR.00009-06.PubMed CentralView ArticlePubMed
- Huang G: Regulation of phenotypic transitions in the fungal pathogen Candida albicans. Virulence. 2012, 3: 251-261. 10.4161/viru.20010.PubMed CentralView ArticlePubMed
- Martin R, Albrecht-Eckardt D, Brunke S, Hube B, Hünniger K, Kurzai O: A core filamentation response network in Candida albicans is restricted to eight genes. PLoS One. 2013, 8: e58613-10.1371/journal.pone.0058613.PubMed CentralView ArticlePubMed
- Ramage G, VandeWalle K, López-Ribot JL, Wickes BL: The filamentation pathway controlled by the Efg1 regulator protein is required for normal biofilm formation and development in Candida albicans. FEMS Microbiol Lett. 2002, 214: 95-100. 10.1111/j.1574-6968.2002.tb11330.x.View ArticlePubMed
- Argimón S, Wishart JA, Leng R, Macaskill S, Mavor A, Alexandris T, Nicholls S, Knight AW, Enjalbert B, Walmsley R, Odds FC, Gow NA, Brown AJ: Developmental regulation of an adhesin gene during cellular morphogenesis in the fungal pathogen Candida albicans. Eukaryot Cell. 2007, 6: 682-692. 10.1128/EC.00340-06.PubMed CentralView ArticlePubMed
- Rodier MH, Imbert C, Kauffmann-Lacroix C, Daniault G, Jacquemin JL: Immunoglobulins G could prevent adherence of Candida albicans to polystyrene and extracellular matrix components. J Med Microbiol. 2003, 52 (Pt 5): 373-377.View ArticlePubMed
- Tsai PW, Yang CY, Chang HT, Lan CY: Human antimicrobial peptide LL-37 inhibits adhesion of Candida albicans by interacting with yeast cell-wall carbohydrates. PLoS One. 2011, 6: e17755-10.1371/journal.pone.0017755.PubMed CentralView ArticlePubMed
- Ardehali R, Shi L, Janatova J, Mohammad SF, Burns GL: The inhibitory activity of serum to prevent bacterial adhesion is mainly due to apo-transferrin. J Biomed Mater Res A. 2003, 66: 21-28.View ArticlePubMed
- Finkel JS, Mitchell AP: Genetic control of Candida albicans biofilm development. Nat Rev Microbiol. 2011, 9: 109-118. 10.1038/nrmicro2475.PubMed CentralView ArticlePubMed
- Nobile CJ, Schneider HA, Nett JE, Sheppard DC, Filler SG, Andes DR, Mitchell AP: Complementary adhesin function in C. albicans biofilm formation. Curr Biol. 2008, 18: 1017-1024. 10.1016/j.cub.2008.06.034.PubMed CentralView ArticlePubMed
- Finkel JS, Xu W, Huang D, Hill EM, Desai JV, Woolford CA, Nett JE, Taff H, Norice CT, Andes DR, Lanni F, Mitchell AP: Portrait of Candida albicans Adherence Regulators. PLoS Pathog. 2012, 8: e1002525-10.1371/journal.ppat.1002525.PubMed CentralView ArticlePubMed
- Nobile CJ, Mitchell AP: Regulation of cell-surface genes and biofilm formation by the C. albicans transcription factor Bcr1p. Curr Biol. 2005, 15: 1150-1155. 10.1016/j.cub.2005.05.047.View ArticlePubMed
- Hoyer LL: The ALS gene family of Candida albicans. Trends Microbiol. 2001, 9: 176-180. 10.1016/S0966-842X(01)01984-9.View ArticlePubMed
- Sheppard DC, Yeaman MR, Welch WH, Phan QT, Fu Y, Ibrahim AS, Filler SG, Zhang M, Waring AJ, Edwards JE: Functional and structural diversity in the Als protein family of Candida albicans. J Biol Chem. 2004, 279: 30480-30489. 10.1074/jbc.M401929200.View ArticlePubMed
- Zhao X, Oh SH, Yeater KM, Hoyer LL: Analysis of the Candida albicans Als2p and Als4p adhesins suggests the potential for compensatory function within the Als family. Microbiology. 2005, 151 (Pt 5): 1619-1630.PubMed CentralView ArticlePubMed
- Bastidas RJ, Heitman J, Cardenas ME: The protein kinase Tor1 regulates adhesin gene expression in Candida albicans. PLoS Pathog. 2009, 5: e1000294-10.1371/journal.ppat.1000294.PubMed CentralView ArticlePubMed
- Sundstrom P: Adhesion in Candida spp. Cell Microbiol. 2002, 4: 461-469. 10.1046/j.1462-5822.2002.00206.x.View ArticlePubMed
- Nobile CJ, Nett JE, Andes DR, Mitchell AP: Function of Candida albicans Adhesin Hwp1 in Biofilm Formation. Eukaryot Cell. 2006, 5: 1604-1610. 10.1128/EC.00194-06.PubMed CentralView ArticlePubMed
- Ramage G, Vande Walle K, Wickes BL, López-Ribot JL: Standardized method for in vitro antifungal susceptibility testing of Candida albicans biofilms. Antimicrob Agents Chemother. 2001, 45: 2475-2479. 10.1128/AAC.45.9.2475-2479.2001.PubMed CentralView ArticlePubMed
- Chandra J, Mukherjee PK, Ghannoum MA: In vitro growth and analysis of Candida biofilms. Nat Protoc. 2008, 3: 1909-1924. 10.1038/nprot.2008.192.View ArticlePubMed
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.