Erratum to: Molecular analysis of subtilase cytotoxin genes of food-borne Shiga toxin-producing Escherichia coli reveals a new allelic subAB variant

  • Joschua Funk1,

    Affiliated with

    • Helen Stoeber1,

      Affiliated with

      • Elisabeth Hauser1 and

        Affiliated with

        • Herbert Schmidt1Email author

          Affiliated with

          BMC Microbiology201414:32

          DOI: 10.1186/1471-2180-14-32

          Received: 16 January 2014

          Accepted: 16 January 2014

          Published: 10 February 2014

          The original article was published in BMC Microbiology 2013 13:230

          Correction

          After publication of this work [1], we noticed that the sequences of primers subA-L and subAB5’-OEP in Table two (Table 1 here) are not correct. We therefore provide the correct primer sequences in a new Table two (Table 1 here).
          Table 1

          Designations, targets, and positions of primers for PCR analysis and Southern blot hybridization

          Primer

          Target

          Sequence (5′ – 3′)

          GenBank accession number

          Reference

          saaDF

          saa

          5′-CGT GAT GAA CAG GCT ATT GC-3′

          AF399919

          [26]

          saaDR

          saa

          5′-ATG GAC ATG CCT GTG GCA AC-3′

          AF399919

          [26]

          subAB-V-for

          subAB

          5′-CTT CCC TCA TTG CCT CAC G-3′

          AY258503

          This study

          subAB-V-rev

          subAB

          5′-GGC TGG CCT GTT GTG TAA A-3′

          AY258503

          This study

          tia_lo

          tia

          5′-TCC ATG CGA AGT TGT TAT CA-3′

          U20318

          [15]

          tia_sense

          tia

          5′-TTC TCT TTT TAC CCT GCT TTT TGC-3′

          FJ664545

          [15]

          subAB-for5

          subAB 1

          5′-CGT ATC TGC GCC ATA TCC TG-3′

          AY258503

          This study

          subAB-rev5

          subAB 1

          5′-CTG TTC CGA GCA GCC ATA TC-3′

          AY258503

          This study

          subAB3′tia

          subAB 2-1

          5′-ACT GGC TGT TCT AAC CG-3′

          AEZO02000028.1

          This study

          subA_out

          subA 2-2

          5′-GAA TCA ACA ACA GAT ACG AC-3′

          AEZO02000020.1

          This study

          subA-L

          Linkera

          5′-GGG AGG ATT AAC CAT CG-3′

          AEZO02000020.1

          This study

          subAB5′OEP

          subAB 2-2

          5′-ATG AAT GAG AGC ATC CCT-3′

          AEZO02000020.1

          This study

          subAB2-3′out

          subAB 2-2

          5′-AGG TCG GCT CAG TGT TC-3′

          AEZO02000020.1

          This study

          aIntergenic linker between the OEP-locus and subA 2-2 .

          Notes

          Authors’ Affiliations

          (1)
          Department of Food Microbiology, Institute of Food Science and Biotechnology, University of Hohenheim

          References

          1. Funk J, Stoeber H, Hauser E, Schmidt H: Molecular analysis of subtilase cytotoxin genes of food-borne shiga toxin-producing escherichia coli reveals a new allelic subAB variant. BMC Microbiol 2013, 13:230. 10.1186/1471-2180-13-230PubMed CentralPubMedView Article

          Copyright

          © Funk et al.; licensee BioMed Central Ltd. 2014

          This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://​creativecommons.​org/​licenses/​by/​2.​0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://​creativecommons.​org/​publicdomain/​zero/​1.​0/​) applies to the data made available in this article, unless otherwise stated.

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