Transcriptomic clues to understand the growth of Lactobacillus rhamnosus in cheese
© Lazzi et al.; licensee BioMed Central Ltd. 2014
Received: 24 September 2013
Accepted: 3 February 2014
Published: 7 February 2014
Lactobacillus rhamnosus is a non-starter lactic acid bacterium that plays a significant role during cheese ripening, leading to the formation of flavor. In long-ripened cheeses it persists throughout the whole time of ripening due to its capacity to adapt to changing environmental conditions. The versatile adaptability of L. rhamnosus to different ecosystems has been associated with the capacity to use non-conventional energy sources, regulating different metabolic pathways. However, the molecular mechanisms allowing the growth of L. rhamnosus in the cheese dairy environment are still poorly understood. The aim of the present study was to identify genes potentially contributing to the growth ability of L. rhamnosus PR1019 in cheese-like medium (CB) using a transcriptomic approach, based on cDNA-amplified fragment length polymorphism (cDNA-AFLP) and quantitative real-time reverse transcription-PCR (qPCR).
Using three primer combinations, a total of 89 and 98 transcript-derived fragments were obtained for L. rhamnosus PR1019 grown in commercial MRS medium and CB, respectively. The cDNA-AFLP results were validated on selected regulated genes by qPCR. In order to investigate the main adaptations to growth in a cheese-mimicking system, we focused on 20 transcripts over-expressed in CB with respect to MRS. It is worth noting the presence of transcripts involved in the degradation of pyruvate and ribose. Pyruvate is a intracellular metabolite that can be produced through different metabolic routes starting from the carbon sources present in cheese, and can be released in the cheese matrix with the starter lysis. Similarly the ribonucleosides released with starter lysis could deliver ribose that represents a fermentable carbohydrate in environments, such as cheese, where free carbohydrates are lacking.
Both pyruvate degradation and ribose catabolism induce a metabolite flux toward acetate, coupled with ATP production via acetate kinase. Taking into account these considerations, we suggest that the energy produced through these pathways may concur to explain the great ability of L. rhamnosus PR1019 to grow on CB.
By a transcriptomic approach we identified a set of genes involved in alternative metabolic pathways in L. rhamnosus that could be responsible for L. rhamnosus growth in cheese during ripening.
KeywordsLactobacillus rhamnosus Cheese cDNA-amplified fragment length polymorphism Quantitative real-time reverse transcription-PCR
Lactobacillus rhamnosus is a facultatively heterofermentative Lactic Acid Bacterium (LAB) frequently encountered in many dairy products, where it can be added as a probiotic microorganism or can be naturally present arising from raw milk. LAB may play different roles in cheese manufacture: some species participate in the fermentation process and contribute to acid production acting as starter LAB (SLAB), whereas others, called non-starter LAB (NSLAB), are mainly implicated in the maturation process. In particular, L. rhamnosus plays a significant role during ripening, leading to the formation of flavor [1, 2] and, for this reason, members of this species are generally recognized as NSLAB. It is noteworthy that NSLAB generally have a high tolerance to hostile environments, such as those with high salt concentration, low moisture, 4.9-5.3 pH values, low temperatures and deficiency of nutrients [3–5]. Moreover, several studies have reported that in long-ripened cheese varieties, NSLAB populations dominate during aging after SLAB decline due to autolysis [6, 7]. Increasing by about four to five orders of magnitude within a few months, NSLAB can have a major impact in determining curd maturation and final characteristics of cheese . In particular, L. rhamnosus has been shown to become dominant within NSLAB population in several cheeses, including Parmigiano Reggiano (PR) [8, 9]. It persists throughout the whole time of PR cheese ripening (1 to 20 months) and this implies its capacity to adapt to changing environmental conditions . Notably, different L. rhamnosus strains have been detected in relation to specific steps of cheese ripening, suggesting that these strains may have specific metabolic activities, which could account for the adaptation to the changing microenvironment of cheese ripening .
Up to date, the molecular mechanisms allowing the growth of L. rhamnosus in cheese are still poorly understood. NSLAB development during ripening can be attributed to their ability to use the major nutrient sources available in ripened cheese, that is lactose-free. The nutrient sources available include milk components modified by technological treatment (rennet addition and curd cooking) and starter LAB development, starter LAB metabolites and cell lysis products. Potential substrate for microbial growth are represented by small peptides or amino acids , citrate, lactate, and free fatty acids . Additionally, sugars and phospholipids, nucleic acids and peptides can be released in the cheese matrix when SLAB autolysis starts to occur [13–15]. These compounds represent carbon sources that could yield intracellular pyruvate (i.e. through metabolism of citrate, lactate, amino acids, and nucleotides) or be converted into different metabolites.
To investigate the metabolic pathways occurring in L. rhamnosus during cheese ripening, Bove and colleagues  recently compared the proteomic profiles of 10 L. rhamnosus strains grown in MRS and a cheese-like medium (Cheese Broth, CB). Differently from MRS, which is the standard laboratory medium for lactobacilli  and is considered to be a rich substrate with glucose as the primary carbon source for microbial growth, CB is an experimental medium formulated with 20-month-ripened PR cheese [10, 16, 18], that tries to mimic the nutritional composition of PR cheese during ripening. PR cheese, and thus CB, is considered to be a substrate poor in carbohydrates and characterized by the absence of milk sugar, lactose. During the curd acidification step of PR cheese production, the conversion of lactose into lactic acid is the main biochemical process that occurs. Lactose is completely depleted within 24 to 48 h . The composition of CB, prepared as the protocol of Neviani et al. , is the following (g/l): proteins, 80.92; lactose, 0.00; glucose, 0.00; galactose, 0.00; lactic acid, 3.82; NaCl, 3.4; sodium citrate, 20.64.
According to the findings of Bove et al.  compared to the cultivation in MRS, the differentially expressed proteins under cheese-like conditions were mainly linked to protein biosynthesis and catabolism, nucleotide and carbohydrate metabolisms, citrate metabolism, cell wall and exopolysaccharide biosynthesis, cell regulation, oxidation/reduction processes, and stress response . Notably, L. rhamnosus produced lactic acid as the primary end product when growing in MRS, whereas in CB low levels of lactic acid together with high levels of acetic acid were detected for all strains. Despite this common trend, the authors also observed strain-specific physiological responses, suggesting a strain variability in the adaptation to changing environmental conditions in accordance with genetic polymorphism studies . Among all strains, L. rhamnosus PR1019, isolated from PR 4-month-ripened cheese, has shown the greatest ability to growth in CB reaching after 48 h the highest cell numbers with one of the lowest levels of lactic acid and one of the highest levels of acetic acid.
For this reason, in the present work, we focused our attention only on this strain, with the aim to identify the genes that could concur to explain its growth ability in CB and its acid acetic production. The physiological adaptation of L. rhamnosus PR1019 in CB was evaluated using a transcriptomic approach, based on cDNA-amplified fragment length polymorphism (cDNA-AFLP) and quantitative real-time reverse transcription-PCR (qPCR). cDNA-AFLP is one of the most robust and sensitive transcriptomic technologies for genome-wide expression studies, with the main advantage of not requiring any prior knowledge of gene sequences while allowing the detection of lowly expressed genes through transcript amplification .
Using this approach, we identified a set of genes resulted over-expressed in CB compared to MRS, potentially involved in alternative metabolic pathways. Interesting genes were searched in other NSLAB and SLAB genomes with the aim to explore their diversity. Overall, the results described in this work highlight mechanisms of adaptation leading to the production of acetic acid coupled with ATP generation, that could support the L. rhamnosus growth in cheese during ripening.
Bacterial growth conditions
L. rhamnosus PR1019 was isolated from Parmigiano Reggiano (PR) at 4 months of ripening on cheese based medium  plate counts and identified by 16S rDNA gene sequencing  and species-specific PCR . The strain was cultivated in MRS broth (Oxoid) or Cheese Broth (CB) at 30°C, under anaerobiosis, for 24 or 48 h, respectively. CB, a culture medium that mimics raw-milk long-ripened cheese, was prepared according to the modified protocol described by Bove et al. [16, 18].
RNA extraction and cDNA synthesis
The growth of L. rhamnosus PR1019 in MRS and CB broth was monitored by measuring optical density (OD) at 600 nm. About 109 cells at the top of logarithmic phase were harvested, and total RNA, stabilized with RNAprotect Bacteria Reagent (QIAGEN), was isolated using RNeasy Protect Bacteria Mini Kit (QIAGEN). Three independent biological experiment were made. RNA was quantified using a NanoDrop ND-1000 spectrophotometer (NanoDrop Technologies) and visualized by formaldehyde agarose gel electrophoresis according to standard procedures. All RNAs were of sufficient quantity (>350 ng/μl) and high quality (A260/A280 ratio 2.0 to 2.1). After a step of mRNA enrichment and polyadenylation of RNA transcripts, cDNA was synthesized by reverse transcription (RT) using a biotinylated oligo (dT), following the protocol reported by Bove et al. . For qPCR, single-stranded cDNA was prepared in a final volume of 20 μl from total RNA using an oligo (dT) primer and SuperScript™ II reverse transcriptase (Invitrogen), according to the manufacturer’s instructions. Briefly, 12-μl reaction mixtures containing 500 ng of oligo (dT) primer, 2 μg total RNA and 10 nmol dNTP mix in DEPC-treated H2O were heated to 65°C for 5 min, added with 4 μl of 5X First-Strand Buffer (Invitrogen) and 200 nmol DTT, and then incubated at 42°C for 2 min. RT reactions were started by the addition of 200 U of enzyme, incubated at 42°C for 50 min and inactivated by heating at 70°C for 15 min. RT step was carried out in duplicate.
Primer and adaptor sequences
cDNA-AFLP fragment isolation, re-amplification and sequencing
Transcript-derived fragments (TDFs) of interest were cut from polyacrylamide gels as reported by Vuylsteke et al. , resuspended in 100 μl of distilled water and subsequently re-amplified using the re-amplification and selective PCR primers EcoRI-AC/MseI-AT, EcoRI-AT/MseI-AC and EcoRI-AT/MseI-AT (Table 1) according to the origin of cDNA-AFLP fragments. Amplification reactions were performed in a final volume of 50 μl containing 13 μl of resuspended DNA fragment, 25 mM MgCl2, 10X PCR buffer, 2 μM EcoRI-N primer, 2 μM MseI-N primer, 5 mM dNTPs, 0.5 μl of AmpliTaq 360 DNA polymerase (5U/μl) and 2 μl of 360 GC enhancer (Applied Biosystems-Life Technologies). PCR consisted of: i) 30 s of denaturation step at 94°C, 30 s of annealing step at 65°C (reduced of 0.7°C at each cycle) and 1 min of extension step at 72°C for 13 total cycles; ii) 10 s of denaturation step at 94°C, 30 s of annealing step at 56°C and 1 min of extension step at 72°C (extended for 1 min at each cycle) for 35 total cycles. These cycles were preceded by a common denaturation step of 2 min at 94°C and followed by a final 10-min extension at 72°C, and were carried out in a Mastercycler ep gradient S thermal cycler (Eppendorf). Amplified products were checked on a 1% agarose gel with a 100-bp marker (Invitrogen) and subsequently purified using the Wizard® SV Gel and PCR Clean-Up System according to the manufacturer’s instructions (Promega Corporation). Amplified fragments were then cloned in E.coli using the pGEM-T Easy Vector System kit (Promega Corporation), and plasmids from selected clones were purified using PureYield MiniPrep System kit (Promega Corporation) referring to the producer’s manual. Cloned fragments were finally sequenced by Eurofins MWG Operon using primers M13 and sequences were analysed by BLAST alignment . TDF sequences were deposited in the DDBJ database under the accession numbers AB896768 to AB896786.
qPCR and data processing
qPCR settings and relative transcript abundance
Primer sequence (5′-3′)a
Product size (bp)
In silico analysis
TDF sequences were annotated using BLAST search. Pathway assignment was performed according to COG (Cluster of Orthologous Groups)  functional categories and KEGG (Kyoto Encyclopedia of Genes and Genome)  pathway database. Gene synteny across NSLAB and SLAB genomes was explored through the web server SyntTax . Genome mining for promoter and terminator elements was performed using PePPER toolbox . Translated protein sequences were subjected to Pfam motif analysis . Protein alignments were performed using ClustalW2  and used for phylogenetic tree construction at the Interactive Tree of Life . Multisequence amino acid alignments were represented using CLC-Bio sequence viewer .
Results and discussion
In this study, the cDNA-AFLP technique  was applied to profile the transcriptome of a L. rhamnosus strain grown in conditions mimicking cheese ripening. Despite it is not widely used in bacteria, cDNA-AFLP can be considered an ideal system for genome-wide expression analysis, mainly for the detection of lowly expressed genes.
Identification of TDFs over-expressed in CB
Twenty TDFs strongly over-expressed by L. rhamnosus in CB compared to MRS were extracted from gel and used as templates for re-amplification by PCR.
Direct sequencing of the PCR products failed probably because the sequences were contaminated with co-migrating bands or were too small (data not shown). Products from bands were then cloned and PCR amplicons from 10 individual colonies were sequenced. Reliable (> 100 bp) sequences were obtained for 19 of the 20 TDFs. Each sequence was identified by similarity search using the BLAST program against the GenBank non-redundant (nr) public sequence database.
Transcript-derived fragments (TDFs) from L. rhamnosus PR1019 over-expressed in CB compared to MRS
Max identity - E-valuec
Guanylate kinase (EC 220.127.116.11)
L. rhamnosus GG
98% - 1e-84
ko00230: Purine metabolism
Putative phosphoketolase (EC 18.104.22.168)
L. rhamnosus GG
99% - 3e-57
ko00030: Pentose phosphate pathway
L. rhamnosus Lc 705
95% - 6e-17
COG2329: Conserved protein involved in polyketide biosynthesis related to monooxygenase [R]
77% - 2e-07
Lipoteichoic acid synthase LtaS Type IIa (EC 3.1.6)
L. rhamnosus Lc 705
100% - 5e-43
COG1368: Phosphoglycerol transferase and related proteins, alkaline phosphatase superfamily [M]
Conserved hypothetical protein
L. rhamnosus Lc 705
85% - 9e-27
L-xylulose 5-phosphate 3-epimerase (EC 22.214.171.124)
L. rhamnosus GG
94% - 9e-13
ko00040: Pentose and glucuronate interconversions
Pyruvate oxidase (EC 126.96.36.199)
L. rhamnosus GG
93% - 5e-40
COG3961: Pyruvate decarboxylase and related thiamine pyrophosphate-requiring enzymes [G]
ko00620: Pyruvate metabolism
98% - 4e-68
96% - 3e-64
97% - 9e-48
96% - 4e-10
Imidazoleglycerol-phosphate dehydratase (EC 188.8.131.52)
L. rhamnosus Lc 705
92% - 5e-08
ko00340: Hystidine metabolism
96% - 5e-23
Calcineurin-like phosphoesterase family protein
L. rhamnosus ATCC 8530
97% - 3e-70
COG0737: 5′-nucleotidase/2′,3′-cyclic phosphodiesterase and related esterases [F]
98% - 1e-68
L. rhamnosus GG
98% - 5e-34
5S ribosomal RNA
L. rhamnosus GG
98% - 2e-11
5S ribosomal RNA
L. rhamnosus GG
98% - 4e-09
It is known that plasmids often carry genes that might be essential for survival under harsh conditions, encoding important traits, such as enzymes involved in secondary metabolic pathways . Plasmids are known to be a source of LAB genetic and phenotypic diversity which occasionally confers adaptive advantages to host strains . However, further studies are clearly needed to better explore the role of plasmid sequences in the L. rhamnosus adaptation to the cheese ripening environment. To validate the cDNA-AFLP expression profiles, 3 genes, encoding pyruvate oxidase (spxB), L-xylulose 5-phosphate 3-epimerase (ulaE), and xylulose-5-phosphate phosphoketolase (xfp) were selected for qPCR. The relative mRNA abundances were normalized by that of the commonly used reference gene 16S rDNA, and expressed as a ratio of CB to MRS levels. Amplification efficiency for all assays ranged between 85 and 105%. Confirming the reliability of cDNA-AFLP results, all transcripts were more abundant in CB, with expression ratios over 5-fold (Table 2).
To investigate a possible role for these genes in allowing L. rhamnosus growth in cheese during ripening, in silico analyses were carried out.
In silico analysis of TDF no. 93 (305 bp), encoding 101 amino acid residues, revealed the highest identity in amino acid sequence (93%) with a pyruvate oxidase (SpxB) from L. rhamnosus GG (Table 3). Lower levels of identity were observed for SpxB of other members of L. casei group (L. casei, 79%; L. paracasei subsp. paracasei, 79%; L. zeae, 75%). BLASTX search also returned a number of pyruvate oxidases of other NSLAB, such as L. curvatus (55%), L. buchneri (46%), L. brevis (46%), L. plantarum (41%) and L. pentosus (41%), as well as of non-Lactobacillus bacteria.
To our knowledge, this is the first evidence of activation of the POX pathway in L. rhamnosus. On the contrary, POX activity has been extensively described to date in L. plantarum and involved with acetate production in its survival during the stationary phase of aerobic growth [35–39]. In particular, accumulation of acetate instead of lactate is thought to play a role in ensuring the pH homeostasis with an overall beneficial effect for the cell [37, 40]. The additional ATP generated via ACK has been shown to enhance the biomass production . Interestingly, Lorquet et al.  showed that in the late stationary phase, when the production of acetate stopped, an OD decrease resulting from lytic processes occurred. The hypothesis is that in the absence of ATP production, protons can no longer be extruded by ATPases with a consequent dissipation of the proton motive force, which has been shown to be one of the mechanisms triggering autolysis of gram-positive bacteria.
Interestingly, high levels of acetic acid and low levels of lactic acid have been recently observed in L. rhamnosus strains grown in CB under the same conditions of our study [16, 42] Furthermore, by a proteomic approach, Bove et al.  showed an increase in expression of PTA and ACK, which are involved in the synthesis of acetic acid in a branch of the pyruvate metabolism other than POX pathway (Figure 2), during L. rhamnosus growth in CB compared to MRS. Highlighting a possible alternative route of degradation of pyruvate to acetate (the POX pathway; Figure 2), our transcriptomic results seem to complement data from proteomics, strengthening the hypothesis that L. rhamnosus can utilize pyruvate as a growth substrate during cheese ripening.
Pyruvate is an intracellular metabolite that could be produced through different metabolic routes using the carbon sources present in cheese (i.e. through metabolism of citrate, lactate, amino acids, and nucleotides). Moreover, pyruvate can be released in the cheese matrix with starter lysis. Liu et al.  showed that the activity of POX in L. plantarum could be related to the catabolism of L-serine. According to the authors, L-serine is deaminated via a serine dehydratase into pyruvate, which is subsequently converted into acetate by the POX enzyme . Pyruvate conversion by POX has been recently supposed also in L. casei. Looking at the closely related taxonomic groups of these heterofermentative lactobacilli and considering that they share similar ecological niches, including ripened cheese, it appears interesting to further investigate in the future the role of pyruvate metabolism during cheese ripening.
BLASTX analysis of TDF no. 86 (109 bp), putatively encoding 36 amino acid residues, showed the maximum identity (94%) to a protein annotated as L-xylulose 5-phosphate 3-epimerase (ulaE) from L. rhamnosus GG (Table 3). Eighty-four percent of identity was exhibited to the same putative protein from other L. casei group members (L. casei and L. paracasei subsp. paracasei). Homologues were also found in NSLAB known to play a role in flavor generation and other ripening processes: L. suebicus (74%), L. coryniformis (72%) and Carnobacterium maltaromaticum (69%).
According to SyntTax, regions up and downstream of ulaE gene from L. rhamnosus GG shared a conserved gene order with L. casei ATCC 334, whereas no synteny was found in L. buchneri CD034, L. plantarum WCFS1, L. helveticus R0052, L. delbrueckii subsp. bulgaricus ATCC 11842 and L. brevis ATCC 367 genomes (Figure 3B). According to PePPER analysis of L. rhamnosus GG genome, a potential terminator stem-loop structure was identified 82 bp downstream from the araD gene stop codon. No putative promoters were predicted up to 5000 bp upstream of ulaE gene. Interestingly, the upstream LGG_02727 gene was annotated as a transcriptional regulator, belonging to DeoR family. Phylogenetic analysis of L-xylulose 5-phosphate 3-epimerase homologues revealed that ulaE predicted protein from L. rhamnosus clustered close to the putative enzymes from other L. casei group members and L. coryniformis (Figure 4B). Multiple sequence alignment of TDF 86 and homologs from several NSLAB is shown in Additional file 1: Figure S1B.
TDF no. 40 (302 bp) displayed the highest identity (99%) in amino acid sequence with a putative phosphoketolase (xfp) from L. rhamnosus GG (Table 3). Percentages of identity > 95% were found with other L. casei group members (L. zeae, 98%; L. paracasei subsp. paracasei, 96%; L. casei, 96%). BLASTX search also revealed a significant match to a predicted xylulose-5-phosphate phosphoketolase from L. coryniformis (identity 75%). Interestingly, lower levels of identity were obtained with SLAB, such as L. delbrueckii subsp. bulgaricus (56%), L. delbrueckii subsp. lactis (56%) and L. helveticus (55%).
The over-expression of xfp mRNA levels in L. rhamnosus grown in CB, as found in our study, seems to support this hypothesis. Moreover, our findings are in agreement with the proteomic data of Bove and colleagues , who observed an increase in expression level of ribose-5-phosphate isomerase (Rpi) after L. rhamnosus growth in CB compared to MRS. This enzyme acts in a step upstream of xfp in the pathway that leads from ribose 5-phosphate (R5P) to the production of acetate, catalyzing the conversion of R5P to ribulose 5-phosphate (Figure 5).
According to Pfam search, TDF 40-deduced 100 amino acid sequence contains a portion of the XFP C-terminal domain (pfam09363). The genetic organization and location of xfp gene on L. rhamnosus GG and L. casei ATCC 334 chromosomes were shown to be highly similar (Figure 3C). In particular, xfp genes are preceded by a divergently transcribed ORF, encoding a major facilitator superfamily transporter, and are followed by several genes predicted to encode components of ABC transporter and PTS systems for sugar uptake. According to PePPER, no high-scoring promoter consensus sequences were identified in the 5000-bp upstream region of xfp gene in L. rhamnosus GG. A Rho-independent transcriptional terminator was predicted to occur 43 bp downstream from the xfp stop codon. Phylogram showed that xfp proteins from L. casei group made a separate cluster, close to the putative enzyme from L. coryniformis (Figure 4C). Analogously, different clusters were observed for the SLAB L. helveticus, L. delbrueckii subsp. lactis and L. delbrueckii subsp. bulgaricus. Additional file 1: Figure S1C displays a multiple sequence alignment of TDF 40 and putative phosphoketolases from several SLAB and NSLAB.
In this study, we applied a transcriptomic approach, based on cDNA-AFLP and qPCR, to investigate the physiological adaptation of L. rhamnosus to the cheese environment. L. rhamnosus is known to be one of the few NSLAB species able to survive and grow during long ripening of sseveral cheeses. In particular, the strain L. rhamnosus PR1019, isolated from 4-month-ripened PR cheese, has previously shown a great ability to growth in CB coupled with high levels of production of acetic acid. By comparing the gene expression profiles of L. rhamnosus PR1019 in CB respect to MRS, we identified among others as over-expressed in CB, genes linked to the conversion of pyruvate to acetate as well as to the pathway of ribose degradation. Notably, the activation of POX pathway in L. rhamnosus has never been observed before.
Pyruvate is a intracellular metabolite that could be produced by different metabolism using the carbon source present in cheese and can be released in the cheese matrix with the starter lysis. Similarly the ribonucleosides release with starter lysis could be carriers of ribose that represents a fermentable carbohydrate in an environments such cheese where carbohydrates are lacking.
Both pyruvate degradation and ribose catabolism induce a metabolite flux toward acetate, coupled with ATP production via acetate kinase. Taking into account these consideration, and in agreement with previous findings  we assume that L. rhamnosus when growing in media poor in carbohydrates, such as CB, arguably uses different metabolic pathways to produce energy. Notably, the transcriptomic approach employed in this study evidenced the over-expression in CB of enzymes other than those identified through proteomics by Bove et al. , acting at different steps or in different branches of the ribose and pyruvate utilization pathways. This discrepancy, probably owing to issues of technique sensitivity and resolution, highlighted the need to integrate transcriptomic and proteomic data in order to get a view as complete as possible of the L. rhamnosus metabolic adaptations during cheese ripening.
Since, to our knowledge, this is the first study that showed the activation of POX pathway in L. rhamnosus, further work will be directed to investigate more in depth the role of the pyruvate metabolism in the growth of this specie in cheese.
Lactic acid bacteria
Starter lactic acid bacteria
Non-starter lactic acid bacteria
cDNA-amplified fragment length polymorphism
Quantitative real-time reverse transcription-PCR
L-xylulose 5-phosphate 3-epimerase
The authors are grateful to Dr. Claudio Giorgio Bove for technical assistance.
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