Role of the fission yeast cell integrity MAPK pathway in response to glucose limitation
© Madrid et al.; licensee BioMed Central Ltd. 2013
Received: 18 October 2012
Accepted: 5 February 2013
Published: 11 February 2013
Glucose is a signaling molecule which regulates multiple events in eukaryotic organisms and the most preferred carbon source in the fission yeast Schizosaccharomyces pombe. The ability of this yeast to grow in the absence of glucose becomes strongly limited due to lack of enzymes of the glyoxylate cycle that support diauxic growth. The stress-activated protein kinase (SAPK) pathway and its effectors, Sty1 MAPK and transcription factor Atf1, play a critical role in the adaptation of fission yeast to grow on alternative non-fermentable carbon sources by inducing the expression of fbp1 + gene, coding for the gluconeogenic enzyme fructose-1,6-bisphosphatase. The cell integrity Pmk1 pathway is another MAPK cascade that regulates various processes in fission yeast, including cell wall construction, cytokinesis, and ionic homeostasis. Pmk1 pathway also becomes strongly activated in response to glucose deprivation but its role during glucose exhaustion and ensuing adaptation to respiratory metabolism is currently unknown.
We found that Pmk1 activation in the absence of glucose takes place only after complete depletion of this carbon source and that such activation is not related to an endogenous oxidative stress. Notably, Pmk1 MAPK activation relies on de novo protein synthesis, is independent on known upstream activators of the pathway like Rho2 GTPase, and involves PKC ortholog Pck2. Also, the Glucose/cAMP pathway is required operative for full activation of the Pmk1 signaling cascade. Mutants lacking Pmk1 displayed a partial growth defect in respiratory media which was not observed in the presence of glucose. This phenotype was accompanied by a decreased and delayed expression of transcription factor Atf1 and target genes fbp1+ and pyp2+. Intriguingly, the kinetics of Sty1 activation in Pmk1-less cells was clearly altered during growth adaptation to non-fermentable carbon sources.
Unknown upstream elements mediate Pck2-dependent signal transduction of glucose withdrawal to the cell integrity MAPK pathway. This signaling cascade reinforces the adaptive response of fission yeast to such nutritional stress by enhancing the activity of the SAPK pathway.
KeywordsFission yeast Glucose MAPK Pmk1 Sty1
In addition to its role as energy source, glucose is a powerful signaling molecule which modulates many cellular responses in eukaryotic organisms, ranging from cell cycle control and differentiation to transcriptional and translational regulation . The regulatory pathways involved in such signaling become particularly patent in simple eukaryotic organisms like budding or fission yeasts, where this sugar is the preferred carbon source for vegetative growth . In the fission yeast Schizosaccharomyces pombe glucose may be fermented under aerobic conditions (Crabtree effect), and a reduction in its concentration strongly affects cell metabolism and gene expression . Moreover, fission yeast cells lack enzymes of the glyoxylate cycle to maintain diauxic growth in the absence of glucose, and this feature limits to glycerol or gluconate their ability to grow on non-sugar carbon sources [4, 5]. Hence, as soon as glucose disappears and respiration of the fermentation products becomes impaired S. pombe undergoes a nutritional stress .
Evidence has accumulated to support a key role of mitogen-activated protein kinase (MAPK) signaling pathways in the response of eukaryotic cells against environmental alterations and stress conditions . In particular, the stress-activated protein kinase (SAPK) pathway, which is one of the three MAPK cascades present in fission yeast, plays a critical function during the modulation of the general cellular response to stress. The central element of this pathway is MAPK Sty1, ortholog to other SAPK members in mammalian cells like p38 and JNK, which results activated in response to multiple stressful conditions [7, 8]. A main target of the SAPK pathway is transcription factor Atf1, a protein containing a leucine zipper domain (bZIP) and homologue to transcriptional factor ATF-2 of higher cells, which associates in vivo to, and is phosphorylated by Sty1 during stress . Activated Atf1 induces the expression of a group of genes forming part of the Core Environmental Stress Response (CESR), whose products participate in the adaptive cell response . Glucose starvation is an environmental stress able to activate the SAPK pathway in S. pombe[11, 12], and mutants lacking either Sty1 or Atf1 are unable to grow on alternative non-fermentable carbon sources due to failure to induce the fbp1+ gene, coding for the gluconeogenic enzyme fructose-1,6-bisphosphatase . Expression of this gene becomes strongly induced by activated Atf1 in the absence of glucose, whereas high glucose concentrations promote increased intracellular cAMP levels and full repression of fbp1+ due to the activity Pka1, the catalytic subunit of protein kinase A . Pka1 phosphorylates and negatively regulates the activity of Rst2, a transcription factor which, together with Atf1, is responsible for the induced expression of fbp1+ when glucose is missing .
The cell integrity pathway is another MAPK cascade that in S. pombe regulates processes like cell wall construction and maintenance during stress, vacuole fusion, cytokinesis, morphogenesis, and ionic homeostasis [8, 15, 16]. Pmk1, the effector MAPK of this signaling module which also includes Mkh1 (MAPKKK) and Pek1/Skh1 (MAPKK), is ortholog to human ERK1/2, and becomes activated in response to a variety of adverse osmotic conditions, cell wall damage, oxidative stress, and glucose withdrawal [17, 18]. Rho2, one of the six Rho GTPases found in fission yeast proteome (Rho1 to Rho5, and Cdc42), is a main positive upstream regulator of the cell integrity pathway whose activity is mediated through Pck2, one of the two orthologs of protein kinase C (PKC) present in this organism [18, 19]. However, although Rho2 and Pck2 are the only known upstream activators of Pmk1, the existence of Pmk1 activity in the absence of both components indicates that the MAPK cascade is branched, with other elements acting upstream this pathway . Some studies have suggested that the essential GTPase Rho1 might also modulate the activity Pmk1 by acting upstream of Pck2 . The fact that both Sty1 and Pmk1 are activated in response to similar stimuli suggests the existence of cross-talk between both signaling cascades. In this context, we have shown that MAPK phosphatases Pyp1, Pyp2, and Ptc1 and Ptc3, whose transcriptional induction is dependent on Sty1-Atf1 function, associate in vivo and dephosphorylate activated Pmk1 . Also, Atf1, which is the main target of Sty1, is phosphorylated by Pmk1 under cell wall damage, although the number of identified genes whose expression is induced in a Pmk1-Atf1-dependent fashion appears to be scarce [8, 22]. In this work we investigated the role of the cell integrity pathway during glucose exhaustion in fission yeast. The results suggest that a specific mechanism regulates MAPK function during this particular stress and unveil the existence of a new crosstalk mechanism whereby activated Pmk1 reinforces growth adaptation to alternative carbon sources by enhancing the activity of the SAPK pathway.
Pmk1 activation in response to glucose deprivation
In fission yeast glucose deprivation triggers a moderate endogenous oxidative stress which is followed by the induced expression of genes like gpx1+ (glutathione peroxidase) and ctt1+ (cytoplasmic catalase). These products play a critical role in the removal of intracellular hydrogen peroxide arising in the change from fermentative to respiratory metabolism . However, preincubation of cell cultures in the presence of N-acetyl cysteine (NAC), a known scavenger for peroxide radicals, did not affect Pmk1 activation in response to glucose withdrawal (Figure 1B). Identical results were obtained when employing other antioxidants like glutathione or alpha-tocopherol (not shown). Hence, Pmk1 activation in the absence of glucose appears due to the lack of this particular carbon source, and unrelated to endogenous oxidative stress.
A novel mechanism is responsible for Pmk1 activation in response to glucose deprivation
Notably, MAPK activation was strongly compromised in a mutant lacking Pck2 and slightly affected in Pck1-less cells, whereas simultaneous deletion of rho2+ in either pck2Δ or pck1Δ cells did not significantly alter the activation response shown by the single mutants (Figure 2A). These results suggest that Pck2 is the key element involved in full signal transmission of glucose deprivation to the Pmk1 cascade. Moreover, as compared to the Rho2-deleted strain, Pmk1 activation in the absence of glucose remained virtually unaffected in control or rho2Δ cells expressing a dominant negative version of Rho1 (T20N) (Figures 2B and 2C), which constitutively binds to GDP and behaves like a lack of function version of this GTPase [23, 24]. Therefore, neither Rho2 nor Rho1 appear to be major determinants in Pck2-dependend signaling to the Pmk1 MAPK cascade in response to glucose exhaustion.
Rho5 GTPase functions in a redundant fashion to Rho1 and plays a nonessential role during stationary phase and in the process of spore wall formation . It is worth to mention that Rho5 levels are almost undetectable in exponentially growing cells, but increase significantly under glucose starvation , thus making this GTPase a potential candidate to modulate Pmk1 activation in a Pck2-dependent fashion. However, as compared to control cells, the enhanced Pmk1 phosphorylation induced by glucose depletion was neither affected by rho5+ deletion nor modified in rho5Δ rho2Δ double mutant cells (Figure 2D). Moreover, simultaneous deletion of rho5+ did not aggravate the defective Pmk1 activation observed in pck2Δ cells (Figure 2E). Notably, Pmk1 activation was still observed in glucose-depleted cells of a rho5Δ mutant expressing a dominant negative allele of Rho1 (T20N) (Figure 2F). This finding rules out the possibility that both GTPases functionally replace each other during signal transduction to the MAPK module. We also observed a clear Pmk1 activation after glucose exhaustion in rho2Δ cells expressing a dominant negative allele of Cdc42 (T17N), which is an essential GTPase involved in the regulation of cell morphogenesis in fission yeast (Figure 2G) . As a whole, the above evidences indicate that Rho1, Rho2, Rho5, or Cdc42 GTPases have not significant role in Pmk1 activation in response to glucose limitation, suggesting the existence of unknown additional element/s to activate the cell integrity MAPK cascade via Pck2 under this condition.
Functional Glucose/cAMP pathway is required for full Pmk1 activation in response to glucose deprivation
Pmk1 activation in response to glucose deprivation requires de novo protein synthesis
Pmk1 reinforces fission yeast adaptive response to metabolic stress imposed by glucose limitation
An attractive possibility about how the cell integrity pathway might favour fission yeast growth during respiration would be that Pmk1 activity positively affects the expression of fructose-1,6-bisphosphatase (fbp1+), whose activity is critical to achieve growth in the absence of glucose . Confirming this prediction, Northern blot experiments showed that the strong increase in fbp1+ expression during growth in a non-fermentable carbon source was decreased and delayed in pmk1Δ cells as compared to control cells (Figure 5B). Since fbp1+ transcriptional activation is positively regulated by the Sty1 pathway through Atf1 transcription factor , we also analyzed the effect of Pmk1 absence in the levels of Pyp2, a tyrosine phosphatase which dephosphorylates both Sty1 and Pmk1, and whose expression is dependent on the Sty1-Atf1 branch [8, 29]. Again, the increased expression of both pyp2+ m-RNA and Pyp2 protein levels was markedly delayed in pmk1Δ cells as compared to control cells after glucose removal (Figures 5B and 5C), thus supporting the conclusion that Pmk1 stimulates the expression of Sty1-Atf1 dependent genes during transition from fermentative to respiratory metabolism.
Because the stress-induced expression of fbp1+ and pyp2+ genes is positively regulated by Sty1 via Atf1, we considered the possibility that the delayed expression of both genes in pmk1Δ cells during the shift to a non-fermentable carbon source might result from an altered kinetics in the activation of the SAPK pathway. Therefore, we comparatively analyzed Sty1 phosphorylation during glucose deprivation in control versus pmk1Δ cells. As shown in Figure 5D, glucose withdrawal induced a quick activation of Sty1 in control cells that was maintained and slowly decreased after 3-4 hours in the presence of non-fermentable carbon sources. However, the kinetics of Sty1 activation in pmk1Δ cells was clearly altered, with a more pronounced dephosphorylation after the initial activation, and the activation maintained for longer times (Figure 5D). Similarly, despite a decreased mobility shift and expression observed early after transfer from fermentative to respiratory medium, Atf1 protein levels (expressed as a genomic copy of the atf1+ gene tagged with two copies of the HA epitope and six histidine residues) remained high in pmk1Δ cells at longer incubation times as compared to control cells (Figure 5E). Notably, the late activation of both Sty1 and Atf1 prompted in the absence of Pmk1 is in good agreement with the delayed expression pattern observed for Fbp1 or Pyp2 (Figures 5B and C). Taken together, these results suggest that in fission yeast Pmk1 positively regulates the timely activation of the SAPK pathway during the switch from fermentative to respiratory metabolism.
Several lines of evidence obtained in this work strongly suggest that the signal for glucose exhaustion is channelled to the Pmk1 MAPK module through a mechanism involving unknown elements. While Rho2 GTPase is fully or partially involved in Pmk1 activation in response to most environmental stresses , stimulation of the MAPK cascade in response to glucose withdrawal is barely dependent on the activity of this GTPase, since in Rho2-less cells Pmk1 is activated similar to wild type cells except for a slower kinetics at earlier times after carbon source depletion. Lack of function or dominant negative mutants in Rho GTPases like Rho5, whose expression is heavily induced after nutrient deprivation , and in Rho1 or Cdc42, which have been mentioned as potential upstream activators of this signaling pathway [17, 20], were able to activate Pmk1 in response to this nutritional stress. Moreover, functional screening of Pmk1 activation in mutants deleted in protein kinases involved in nutrient-sensing events (like Tor1, a member of the TOR2C complex ; Lsk1, which participates in nitrogen starvation responses ; or Ppk9, a Snf1 ortholog required for transcription of glucose-repressed genes in budding yeast ) gave negative results as their potential participation in Pmk1 activation (data not shown). Although the identification of the upstream activator/s of the cell integrity pathway during glucose limitation remains so far elusive, our results indicate that Pck2 is a key element for signal reception and transduction to the Pmk1 cascade under these conditions. This conclusion is consistent with the fact that Pck2 is critical for Pmk1 activation in most of the stresses which activate this signaling pathway . However, the detection of some Pmk1 phosphorylation in pck2Δ cells suggests that alternative element/s might be able to transduce the activation signal to the MAPK module independently on this particular kinase. Pck1 might be such element, due to the slight defect in MAPK activation observed in pck1Δ cells. However, considering that Pck1 negatively regulates both basal and osmostress-induced Pmk1 activity , this would imply that it might be playing either a positive or negative role during signal transmission to the cell integrity pathway depending of the nature of the stressing stimulus.
An interesting finding is the observation that de novo protein synthesis is necessary to allow Pmk1 activation in response to glucose limitation. Importantly, this appears to be a specific requirement, because translational inhibition did not preclude MAPK activation in response to other stimuli like osmostress. In attempts to find out the identity of inducible element/s we focussed our attention on the SAPK pathway, whose activity is essential in fission yeast to promote cellular adaptation and growth in the absence of glucose . However, mutant strains lacking either MAPK Sty1 or Atf1 transcription factor displayed strong Pmk1 activation in response to glucose withdrawal, suggesting that the SAPK pathway does not perform a significant role in this response. On the other hand, the defective Pmk1 phosphorylation shown in strains deleted in key members of the cAMP pathway gives support to the idea that this signaling cascade contributes positively to Pmk1 activation in conditions of glucose deprivation. However, this interpretation is difficult to understand taking into account that both intracellular cAMP levels and Pka1 activity decrease dramatically with shortage of glucose . Moreover, Pmk1 activation during glucose deprivation was still evident in cells lacking Rst2, a transcription factor whose activity is repressed by glucose via Pka1 . In absence of glucose, lack of Pka1-dependent phosphorylation promotes Rst2 nuclear entry to activate the transcription of a specific set of genes whose products are involved in cellular adaptation to stress (e.g. ctt1+) and growth in non-fermentable carbon sources (i.e. fbp1+) . A more likely explanation for the low Pmk1 activation in mutants defective in elements operating upstream Rst2 is that they are constitutively adapted to growth without glucose, and therefore less sensitive to its absence than control or rst2Δ cells. However, fission yeast Pka1 becomes hyperphosphorylated during glucose starvation, and it has been proposed that this modification could serve as a mechanism to induce specific PKA functions under limited cAMP-dependent activity . Therefore, the possibility that Pka1 may be involved in Pmk1 activation in the absence of glucose cannot be completely ruled out.
Although the SAPK pathway is critical for growth of fission yeast in the presence of non-fermentable carbon sources, an important demonstration of this work is that full adaptation to respiratory metabolism also requires an operative cell integrity Pmk1 pathway. The functional relationship between Sty and Pmk1 pathways appears to be rather complex. In addition to glucose depletion, several stressing conditions such as hyperosmotic stress, hypergravity, oxidative stress, or thermal upshifts, induce responses involving activation of both Sty1 and Pmk1 [8, 17, 34], suggesting that the two MAPK cascades show effective cross-talk. As an example, both the basal and the osmostic stress–induced Pmk1 phosphorylation are negatively regulated by the SAPK pathway through Pyp1, Pyp2, and Ptc1 phosphatases . Notably, the fact that the growth defect of cells lacking Pmk1 in the absence of glucose is not as dramatic as in sty1Δ cells, suggest that Pmk1 activity may reinforce Sty1 signaling during the control of cell survival and adaptation to these conditions. Results presented here, as the delayed activation of the Sty1-Atf1 branch in pmk1Δ cells, the resulting defect in the expression of targets like fbp1+ or MAPK phosphatase pyp2+ (and probably others), support this interpretation. Interestingly, Sty1 activation does not become significantly affected in a glucose starved pck2Δ mutant as compared to control cells, and Pck2-less cells do not share the growth defect of pmk1Δ cells in respiratory media (data not shown). Therefore, contrary to its role as a signaling transducer to Pmk1 cascade in response to glucose exhaustion, Pck2 does not appear to participate in fission yeast growth adaptation from fermentative to respiratory metabolism. It has been described that the transcription factor Atf1 is specifically activated by Pmk1 in response to cell wall stress and regulates gene expression of a limited number of genes . The altered kinetics and defective synthesis displayed by Pmk1-less cells allow to consider that Atf1 is targeted by Pmk1 during glucose limitation in addition to Sty1. However, the altered Sty1 phosphorylation shown by pmk1Δ cells also suggests that Pmk1 might regulate signal transduction upstream of Sty1. The identification of specific mechanisms regulating crosstalk between both signaling pathways may deserve further investigations.
In fission yeast the cell integrity pathway and its key member, MAPK Pmk1, become strongly activated in a transient way after glucose exhaustion. Notably, PKC-ortholog Pck2 is determinant for full activation of this signaling cascade whereas other known upstream elements of the pathway like Rho2 GTPase plays a minor role in this response. These findings, together with the observation that de novo protein synthesis is critical for Pmk1 activation, strongly suggest that an unknown branch regulates the signaling of the absence of glucose to the cell integrity pathway. Pmk1 activity is required for fission yeast adaptation from fermentative to respiratory metabolism, as evidenced by the moderate growth defect displayed by Pmk1-less cells in respiratory media. Our results support that Pmk1 reinforces the adaptive response of fission yeast to the nutritional stress by enhancing the activity of the SAPK pathway at two different levels: i- by positively targeting Atf1 transcription factor to allow timely and full expression of genes involved in growth adaptation to respiratory metabolism, and ii- by enhancing signal transmission to Sty1, the core MAPK of the SAPK pathway.
Strains, growth conditions, stress treatments and plasmids
S . pombe strains used in this study*
Madrid et al. 
Madrid et al. 
Madrid et al. 
Madrid et al.
h+pmk1::KanR pmk1(K52E)-GFP:: leu1+
Sánchez-Mir et al. 
Madrid et al.
Sánchez-Mir et al. 
Madrid et al. 
Madrid et al, 
h+rho2:: kanR pmk1-Ha6H:: ura4+
Madrid et al, 
h+pck2:: kanR pmk1-Ha6H::ura4+
Barba et al., 
h+rho2:: kanR pck2:: kanMX6 pmk1- Ha6H:: ura4+
Barba et al., 
Barba et al., 
h-his7-366 atf1-Ha6H:: ura4+
h-his7-366 atf1-Ha6H:: ura4+pmk1::KanR
h+his7-366 sty1-Ha6H:: ura4+
Madrid et al., 
h+rho2:: kanR rho5::natR pmk1-Ha6H:: ura4+
h+rho2:: kanR rho5::natR pmk1-Ha6H:: ura4+
h+rho5::natR pck2:: kanR pmk1-Ha6H::ura4+
Purification and detection of activated Pmk1 and Sty1
Cells from 30 ml of culture were harvested at different times by centrifugation at 4°C, washed with cold PBS buffer, and the yeast pellets immediately frozen in liquid nitrogen. Cell homogenates were prepared under native conditions employing acid-washed glass beads and lysis buffer (10% glycerol, 50 mM Tris-HCl pH 7.5, 150 mM NaCl, 0.1% Nonidet NP-40, plus specific protease and phosphatase inhibitor, Sigma Chemical). The lysates were cleared by centrifugation at 15000 rpm for 20 min, and the proteins were resolved in 10% SDS-PAGE gels, and transferred to nitrocellulose filters (GE Healthcare). The filters were incubated with either monoclonal mouse anti-Ha (clone 12CA5, Roche Molecular Biochemicals), polyclonal rabbit anti-phospho-p42/44 antibodies (Cell Signaling), or monoclonal mouse anti-phospho-p38 antibodies (Cell Signaling) [12, 17]. The immunoreactive bands were revealed with either anti-rabbit or anti-mouse HRP-conjugated secondary antibodies (Sigma Chemical) and the ECL detection kit (GE Healthcare). Quantification of Western blots was performed using Molecular Analyst Software (Bio-Rad).
Purification and detection of Atf1 and Pyp2
For Atf1 purification (expressed as a Atf1-Ha6H fusion), pelleted cells were lysed into denaturing lysis buffer (6 M Guanidine HCl, 0.1 M sodium phosphate, 50 mM Tris HCl, pH 8.0), and the fusion was isolated by affinity precipitation on Ni2+-NTA-agarose beads. The purified protein was resolved in 7% SDS-PAGE gels, transferred to nitrocellulose filters (GE Healthcare), and incubated with a mouse anti-Ha antibody (12CA5). To detect Pyp2 levels (expressed as a Pyp2-13myc fusion) the cleared lysates were prepared under native conditions (see above), resolved in 8% SDS-PAGE gels, transferred to nitrocellulose filters, and incubated with a monoclonal mouse anti-c-myc antibody (clone 9E10, Roche Molecular Biochemicals). Anti-Cdc2 antibody (PSTAIRE; Sigma Chemical) was used as loading control.
Northern blot analysis
Aliquots of the cultures were recovered at different times, total RNA preparations obtained and resolved through 1.5% agarose-formaldehyde gels, and hybridizations were performed as previously described . The probes employed were a 2.1 Kbp fragment of the pyp2+ gene amplified by PCR with the 5′ oligonucleotide CCGAGAGCGTTTCTTGGA and the 3′ oligonucleotide AAGGGCTTGGAAGCCTGG, a 1 Kbp fragment of the fbp1+ gene amplified with the 5′oligonucleotide CTTCCAAGCCAAATACTG and the 3′oligonucleotide GATCTCGACGAAATCGAC, and a 1 Kbp fragment of the leu1+ gene amplified with the 5′ oligonucleotide TCGTCGTCTTACCAGGAG and the 3′ oligonucleotide CAACAGCCTTAGTAATAT. Ready-To-Go DNA labelling beads and the Rapid-Hyb buffer (GE Healthcare) were used for DNA labeling and hybridization, respectively. mRNA levels were quantified in a Phosphorimager (Molecular Dynamics) and compared with the internal control (leu1+ mRNA).
Plate assay of sensitivity for growth
Wild-type and mutant strains of S. pombe were grown in YES liquid medium (7% glucose) to an OD600= 0.6. Appropriate dilutions were spotted per duplicate on YES solid medium supplemented with either 7% glucose or 2% glycerol plus 3% ethanol, and in the presence/absence of 30 mM NAC. Plates were incubated at 28°C for 5 days and then photographed.
Reproducibility of results
All experiments were repeated at least three times. Depending on the experiment, mean relative units + SD and/or representative results are shown.
This work was supported in part by grants from MEC BFU2011-22517 to JC, and 15280/PI/10 from Fundación Séneca, Spain. ERDF (European Regional Development Fund) co-funding from the EU. We thank JB Millar (University of Warwick, United Kingdom) for kind supply of yeast strains, and to F Garro for technical assistance. LSM is a predoctoral fellow (Formación de Personal Investigador) from Ministerio de Economía y Competitividad, Spain. MM is a postdoctoral researcher (Juan de la Cierva Program) from Ministerio de Economía y Competitividad, Spain.
- Rolland F, Winderickx J, Thevelein JM: Glucose-sensing mechanisms in eukaryotic cells. Trends Biochem Sci. 2001, 26: 310-317. 10.1016/S0968-0004(01)01805-9.PubMedView Article
- Gancedo JM: The early steps of glucose signaling in yeast. FEMS Microbiol Rev. 2008, 32: 673-704. 10.1111/j.1574-6976.2008.00117.x.PubMedView Article
- Yanagida M: Cellular quiescence: are controlling genes conserved?. Trends Cell Biol. 2009, 19: 705-715. 10.1016/j.tcb.2009.09.006.PubMedView Article
- Flores CL, Rodriguez C, Petit T, Gancedo C: Carbohydrate and energy-yielding metabolism in non-conventional yeasts. FEMS Microbiol Rev. 2000, 24: 507-529.PubMed
- Van Dijken JP, Weusthuis RA, Peonk JT: Kinetics of growth and sugar consumption in yeasts. Antonie van Leeuwenhoek. 1993, 63: 343-352. 10.1007/BF00871229.PubMedView Article
- Kim EK, Choi EJ: Pathological roles of MAPK signaling pathways in human diseases. Biochim Biophys Acta. 2010, 1802: 396-405. 10.1016/j.bbadis.2009.12.009.PubMedView Article
- Gacto M, Soto T, Vicente-Soler J, Villa TG, Cansado J: Learning from yeasts: intracellular sensing of stress conditions. Int Microbiol. 2003, 6: 211-219. 10.1007/s10123-003-0136-x.PubMedView Article
- Pérez P, Cansado J: Cell integrity signaling and response to stress in fission yeast. Curr Protein Pept Sci. 2010, 11: 680-692. 10.2174/138920310794557718.PubMedView Article
- Wilkinson MG, Samuels M, Takeda T, Shieh JC, Toda T, Millar JB, Jones N: The Atf1 transcription factor is a target for the Sty1 stress-activated MAP kinase pathway in fission yeast. Genes Dev. 1996, 10: 2289-2301. 10.1101/gad.10.18.2289.PubMedView Article
- Chen D, Toone WM, Mata J, Lyne R, Burns G, Kivinen K, Brazma A, Jones N, Bahler J: Global transcriptional responses of fission yeast to environmental stress. Mol Biol Cell. 2003, 14: 214-229. 10.1091/mbc.E02-08-0499.PubMedPubMed CentralView Article
- Shiozaki K, Shiozaki M, Russell P: Mcs4 mitotic catastrophe suppressor regulates the fission yeast cell cycle through the Wik1-Wis1-Spc1 kinase cascade. Mol Biol Cell. 1997, 8: 409-419.PubMedPubMed CentralView Article
- Madrid M, Soto T, Franco A, Paredes V, Vicente J, Hidalgo E, Gacto M, Cansado J: A cooperative role for Atf1 and Pap1 in the detoxification of the oxidative stress induced by glucose deprivation in Schizosaccharomyces pombe. J Biol Chem. 2004, 279: 41594-41602. 10.1074/jbc.M405509200.PubMedView Article
- Neely LA, Hoffman CS: Protein kinase A and mitogen-activated protein kinase pathways antagonistically regulate fission yeast fbp1 transcription by employing different modes of action at two upstream activation sites. Mol Cell Biol. 2000, 20: 6426-6434. 10.1128/MCB.20.17.6426-6434.2000.PubMedPubMed CentralView Article
- Higuchi T, Watanabe Y, Yamamoto M: Protein kinase A regulates sexual development and gluconeogenesis through phosphorylation of the Zn finger transcriptional activator Rst2p in fission yeast. Mol Cell Biol. 2002, 22: 1-11. 10.1128/MCB.22.1.1-11.2002.PubMedPubMed CentralView Article
- Toda T, Dhut S, Superti-Furga G, Gotoh Y, Nishida E, Sugiura R, Kuno T: The fission yeast pmk1+ gene encodes a novel mitogen-activated protein kinase homolog which regulates cell integrity and functions coordinately with the protein kinase C pathway. Mol Cell Biol. 1996, 16: 6752-6764.PubMedPubMed CentralView Article
- Zaitsevskaya-Carter T, Cooper JA: Spm1, a stress-activated MAP kinase that regulates morphogenesis in S.pombe. EMBO J. 1997, 16: 1318-1331. 10.1093/emboj/16.6.1318.PubMedPubMed CentralView Article
- Madrid M, Soto T, Khong HK, Franco A, Vicente J, Pérez P, Gacto M, Cansado J: Stress-induced response, localization, and regulation of the Pmk1 cell integrity pathway in Schizosaccharomyces pombe. J Biol Chem. 2006, 281: 2033-2043.PubMedView Article
- Barba G, Soto T, Madrid M, Núñez A, Vicente J, Gacto M, Cansado J: Activation of the cell integrity pathway is channelled through diverse signalling elements in fission yeast. Cell Signal. 2008, 20: 748-757. 10.1016/j.cellsig.2007.12.017.PubMedView Article
- Ma Y, Kuno T, Kita A, Asayama Y, Sugiura R: Rho2 is a target of the farnesyltransferase Cpp 1 and acts upstream of Pmk1 mitogen-activated protein kinase signaling in fission yeast. Mol Biol Cell. 2006, 17: 5028-5037. 10.1091/mbc.E06-08-0688.PubMedPubMed CentralView Article
- Garcia P, Tajadura V, Sanchez Y: The Rho1p exchange factor Rgf1p signals upstream from the Pmk1 mitogen-activated protein kinase pathway in fission yeast. Mol Biol Cell. 2009, 20: 721-731.PubMedPubMed CentralView Article
- Madrid M, Núñez A, Soto T, Vicente-Soler J, Gacto M, Cansado J: Stress-activated protein kinase-mediated down-regulation of the cell integrity pathway mitogen-activated protein kinase Pmk1p by protein phosphatases. Mol Biol Cell. 2007, 18: 4405-4419. 10.1091/mbc.E07-05-0484.PubMedPubMed CentralView Article
- Takada H, Nishida A, Domae M, Kita A, Yamano Y, Uchida A, Ishiwata S, Fang Y, Zhou X, Masuko T, Kinoshita M, Kakehi K, Sugiura R: The cell surface protein gene ecm33+ is a target of the two transcription factors Atf1 and Mbx1 and negatively regulates Pmk1 MAPK cell integrity signaling in fission yeast. Mol Biol Cell. 2010, 21: 674-685. 10.1091/mbc.E09-09-0810.PubMedPubMed CentralView Article
- Arellano M, Durán A, Pérez P: Localisation of the Schizosaccharomyces pombe rho1p GTPase and its involvement in the organisation of the actin cytoskeleton. J Cell Sci. 1997, 110: 2547-2555.PubMed
- Nakano K, Arai R, Mabuchi I: The small GTP-binding protein Rho1 is a multifunctional protein that regulates actin localization, cell polarity, and septum formation in the fission yeast Schizosaccharomyces pombe. Genes Cells. 1997, 2: 679-694. 10.1046/j.1365-2443.1997.1540352.x.PubMedView Article
- Rincón SA, Santos B, Pérez P: Fission yeast Rho5p GTPase is a functional paralogue of Rho1p that plays a role in survival of spores and stationary-phase cells. Eukaryot Cell. 2006, 5: 435-446. 10.1128/EC.5.3.435-446.2006.PubMedPubMed CentralView Article
- Perez P, Rincón SA: Rho GTPases: regulation of cell polarity and growth in yeasts. Biochem J. 2010, 426: 243-253. 10.1042/BJ20091823.PubMedView Article
- Hoffman CS: Except in every detail: comparing and contrasting G-protein signaling in Saccharomyces cerevisiae and Schizosaccharomyces pombe. Eukaryot Cell. 2005, 4: 495-503. 10.1128/EC.4.3.495-503.2005.PubMedPubMed CentralView Article
- Hoffman CS, Winston F: Glucose repression of transcription of the Schizosaccharomyces pombe fbp1 gene occurs by a cAMP signaling pathway. Genes Dev. 1991, 5: 561-571. 10.1101/gad.5.4.561.PubMedView Article
- Millar JB, Buck V, Wilkinson MG: Pyp1 and Pyp2 PTPases dephosphorylate an osmosensing MAP kinase controlling cell size at division in fission yeast. Genes Dev. 1995, 9: 2117-2130. 10.1101/gad.9.17.2117.PubMedView Article
- Otsubo Y, Yamamoto M: Signaling pathways for fission yeast sexual differentiation at a glance. J Cell Sci. 2012, 125: 2789-2793. 10.1242/jcs.094771.PubMedView Article
- Sukegawa Y, Yamashita A, Yamamoto M: The fission yeast stress-responsive MAPK pathway promotes meiosis via the phosphorylation of Pol II CTD in response to environmental and feedback cues. PLoS Genet. 2011, 7: e1002387-10.1371/journal.pgen.1002387.PubMedPubMed CentralView Article
- Carlson M: Glucose repression in yeast. Curr Opin Microbiol. 1999, 2: 202-207. 10.1016/S1369-5274(99)80035-6.PubMedView Article
- McInnis B, Mitchell J, Marcus S: Phosphorylation of the protein kinase A catalytic subunit is induced by cyclic AMP deficiency and physiological stresses in the fission yeast Schizosaccharomyces pombe. Biochem Biophys Res Commun. 2010, 399: 665-669. 10.1016/j.bbrc.2010.07.139.PubMedPubMed CentralView Article
- Soto T, Núñez A, Madrid M, Vicente J, Gacto M, Cansado J: Transduction of centrifugation-induced gravity forces through mitogen-activated protein kinase pathways in the fission yeast Schizosaccharomyces pombe. Microbiology. 2007, 153: 1519-1529. 10.1099/mic.0.2006/004283-0.PubMedView Article
- Soto T, Beltrán FF, Paredes V, Madrid M, Millar JBA, Vicente-Soler J, Cansado J, Gacto M: Cold induces stress-activated protein kinase-mediated response in the fission yeast Schizosaccharomyces pombe. Eur J Biochem. 2002, 269: 1-10. 10.1046/j.0014-2956.2001.02705.x.View Article
- Sánchez-Mir L, Franco A, Madrid M, Vicente J, Soto T, Pérez P, Gacto M, Cansado J: Biological significance of nuclear localization of MAPK Pmk1 in fission yeast. J Biol Chem. 2012, 287: 26038-26051. 10.1074/jbc.M112.345611.PubMedPubMed CentralView Article
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