Enzymatically active Rho and Rac small-GTPases are involved in the establishment of the vacuolar membrane after Toxoplasma gondii invasion of host cells
- Ren-Hua Na†1,
- Guo-Hui Zhu†1,
- Ji-Xuan Luo1,
- Xiao-Jing Meng2,
- Liwang Cui3,
- Hong-Juan Peng1Email author,
- Xiao-guang Chen1Email author and
- Julian Gomez-Cambronero4
© Na et al.; licensee BioMed Central Ltd. 2013
Received: 10 January 2013
Accepted: 27 May 2013
Published: 30 May 2013
GTPases are the family of hydrolases that bind and hydrolyze guanosine triphosphate. The large Immunity-related GTPases and the small GTPase ADP-ribosylation factor-6 in host cells are known to accumulate on the parasitophorous vacuole membrane (PVM) of Toxoplasma gondii and play critical roles in this parasite infection, but these GTPases cannot explain the full extent of infection.
In this research, RhoA and Rac1 GTPases from the host cell were found to accumulate on the PVM regardless of the virulence of the T. gondii strains after T. gondii invasion, and this accumulation was dependent on their GTPase activity. The real-time micrography of T. gondii tachyzoites invading COS-7 cells overexpressing CFP-RhoA showed that this GTPase was recruited to the PVM at the very beginning of the invasion through the host cell membrane or from the cytosol. Host cell RhoA and Rac1 were also activated after T. gondii tachyzoites invasion, which was needed for host cell cytoskeleton reorganization to facilitate intracellular pathogens invasion. The decisive domains for the RhoA accumulation on the PVM included the GTP/Mg2+ binding site, the mDia effector interaction site, the G1 box, the G2 box and the G5 box, respectively, which were related to the binding of GTP for enzymatic activity and mDia for the regulation of microtubules. The recruited CFP-RhoA on the PVM could not be activated by epithelial growth factor (EGF) and no translocation was observed, unlike the unassociated RhoA in the host cell cytosol that migrated to the cell membrane towards the EGF activation spot. This result supported the hypothesis that the recruited RhoA or Rac1 on the PVM were in the GTP-bound active form. Wild-type RhoA or Rac1 overexpressed cells had almost the same infection rates by T. gondii as the mock-treated cells, while RhoA-N19 or Rac1-N17 transfected cells and RhoA, Rac1 or RhoA + Rac1 siRNA-treated cells showed significantly diminished infection rates compared to mock cells.
The accumulation of the RhoA and Rac1 on the PVM and the requisite of their normal GTPase activity for efficient invasion implied their involvement and function in T. gondii invasion.
KeywordsToxoplasma gondii Parasitophorous vacuole membrane RhoA Rac1 GTPase Accumulate Activate
Toxoplasma gondii is an intracellular protozoan that infects many types of nucleated cells. It is estimated that approximately one-third of the world’s population is chronically infected with tissue cysts of this parasite . Humans may be infected through ingestion of uncooked or under-cooked meat of intermediate hosts or the oocysts excreted by the definitive host, Felis catus. Ingested bradyzoites and tachyzoites invade host cells and cause acute infection. In humans, T. gondii infections may cause disseminating damage to the brain, eyes, lymph nodes and even death in some immunocompromised individuals . In pregnant women, this parasite can be transmitted to the fetus, resulting in tissue destruction, as well as developmental defects of the fetus or newborn . In immunocompetent hosts, tachyzoites are converted into bradyzoites quickly, and a lifelong chronic infection is established.
The molecular mechanism of host cell invasion by T. gondii has been extensively investigated . During invasion, a T. gondii tachyzoite attaches to the host cell membrane and forms a moving junction (MJ) between the tachyzoite and the host cell membrane by releasing microneme proteins (MIC) and rhoptry neck proteins (RON) at the interface of the tachyzoite-host cell surface. Afterwards, the tachyzoite membrane and the host cell membrane remain in contact so that the MJ moves along the parasite’s surface until the parasitophorous vacuole (PV) is finally formed [3, 4]. The MJ works as a sieve to exclude many of the host transmembrane proteins but retains GPI-anchored or raft-associated multipass transmembrane proteins on the PV membrane (PVM) [3, 4]. PVM is a non-fusogenic compartment that is resistant to acidification by the endosome-lysosomal system of the host cell, since most of the PVM is derived from the host cell membrane and the transmembrane proteins, which are involved in fusion with lysosomes and are excluded from the PVM [3–5]. During penetration, the parasite injects many rhoptry proteins including ROP2 into the host cell cytosol, which appear as small satellite vesicles and eventually fuse with the PVM . After invasion, the parasite further modifies the PVM by inserting novel proteins secreted by the rhoptries and the dense granules [7, 8]. After formation, the PVM closely associates with host mitochondria and endoplasmic reticulum (ER) and migrates towards the nucleus using the host microtubule network .
GTPases are a large group of enzymes that bind GTP (guanine triphosphate) and catalyze the hydrolysis of GTP to GDP (guanine diphosphate) in the presence of a Mg2+ ion. They then undergo conformational changes to release GDP, and thus, cycle between a GTP-bound active form and a GDP-bound inactive form . Immune related GTPases (IRG) are large GTPases containing a Ras-like G domain and a helical domain combining N- and C-terminal elements , whereas small GTPases are monomeric GTPases with a molecular weight of 21 kDa and composed of at least five families: Ras, Rho, Rab, Sar1/Arf and Ran, which exist in eukaryotes from yeast to humans . The Rho subfamily is further divided into RhoA, Rac and Cdc42, which regulates cytoskeleton reorganization and gene expression .
A group of interferon-inducible large GTPases (IRGs) and a small GTPase, ADP-ribosylation factor-6 (ARF6) of the host cell accumulate on the PVM of invading T. gondii[14, 15]. IFN-γ-Inducible GTPase (Irga6) is a myristoylated IRG and contributes to resistance against T. gondii in mice. Irga6 is predominantly found in the GDP-bound state in interferon-induced, uninfected cells, but it does accumulate on the PVM after Toxoplasma infection and changes to the GTP-bound form. Accumulation of Irga6 on the T. gondii PVM is associated with vesiculation and ultimately disruption of the vacuolar membrane in a process that requires an intact GTP-binding domain . ARF6 is recruited to the PVM of T. gondii RH strain and plays an important role in the parasite cell invasion with activation of PI3-kinase and recruitment of PIP2 and PIP3 to the PVM of T. gondii. The significance of some GTPases in the Toxoplasma invasion process has prompted us to further investigate whether other members of the small GTPases are also involved in host cell invasion.
KM white mice were purchased from the Laboratory Animal Center of Southern Medical University. Mice were housed in the facility at the School of Public Health and Tropical Medicine according to the guidelines for laboratory animals approved by Guangdong Laboratory Animals Monitoring Institute. This research does not involve human participants, and it was approved by the Institutional Ethics Review Board of Southern Medical University.
Plasmids construction and site mutation
The mutation primers used to generate all the mutants
pECFP-RhoA WT (1-10aa deleted)
pECFP-RhoA WT (11-20aa deleted)
Reverse: 5’- GCTGTTAACTATGAGCAAAATCACCAGTTTCTTCCG-3’
pECFP-RhoA WT (21-30aa deleted)
Reverse: 5’- GCACATACACCTCTGGGCATGTCTTTCCACAG-3’
pECFP-RhoA WT (31-40aa deleted)
Forward: 5’- GCGAGGACCAGTTCAACTATGTGGCAG-3’
Reverse: 5’- CTGCCACATAGTTGAACTGGTCCTCGC-3’
pECFP-RhoA WT (41-50aa deleted)
Reverse: 5’- CCAACTCTACCTGCTTCTCAAACACTGTGGGC-3’
pECFP-RhoA WT (51-60aa deleted)
Mutant 6 Forward: 5’-CGAGGTGGATGGAGCTGGGCTGGAAG-3’
Mutant 6 Reverse: 5’- CTTCCAGCCCAGCTCCATCCACCTCG -3’
pECFP-RhoA WT (61-70aa deleted)
pECFP-RhoA WT (71-80aa deleted)
Reverse: 5’- GATGGAAAAACACATCAGCCTCAGGCGATCATAATC-3’
pECFP-RhoA WT (81-90aa deleted)
pECFP-RhoA WT (91-100aa deleted)
Reverse: 5’- GCTTGACTTCTGGATCAGGGCTGTCG-3’
pECFP-RhoA WT (101-110aa deleted)
pECFP-RhoA WT (111-120aa deleted)
Reverse: 5’- CATCATTCCGAAGCACGTTGGGACAG-3’
pECFP-RhoA WT (121-130aa deleted)
pECFP-RhoA WT (131-140aa deleted)
pECFP-RhoA WT (141-150aa deleted)
Reverse: 5’- CAAAAGCGCCAATTTTCACCGGCTCC-3’
pECFP-RhoA WT (151-160aa deleted)
Reverse: 5’- CCATCTTTGGTCTTTGCCCTGTTTGCCATATCTC-3’
pECFP-RhoA WT (161-170aa deleted)
Reverse: 5’- CGTAGCCATTTCAAATGAACACTCCATGCAC-3’
pECFP-RhoA WT (171-180aa deleted)
Reverse: 5’- CTTCCCACGTCTAGCAACCTCTCTCACTCC-3’
pECFP-RhoA WT (181-192aa deleted)
Reverse: 5’- CGCGGCAAGACAAGTTGCAGAGCAGCTC-3’
Animals, toxoplasma gondii strains and cell lines
Pathogens free (SPF) KM mice were bought from the Animal Institute of Southern Medical University (Guangdong Province, China). T. gondii RH and Pru strain were generous gift from Dr. Xi-Mei Zhan in the School of Medicine of Sun Yat-sen University. The COS-7 cell line was purchased from ATCC and the human bronchial epithelial (16-HBE) cell line was purchased from Shanghai Fuxiang Biotechnology Limited Company. Each cell line was grown in DMEM (Gibco) containing 10% (v/v) NCS (New born calf serum, Gibco) at 5% CO2 and 37°C. For fluorescence microscopy and T. gondii infection rate counting experiments, COS-7 cells were grown on coverslips in the wells of 6-well plates (Corning). 16-HBE cells were used for RNAi and endogenous RhoA and Rac1 immunofluorescence experiments.
Toxoplasma gondii infection
RH strain tachyzoites
Tachyzoites of the RH strain of T. gondii were harvested from the peritoneal cavities of KM mice which were inoculated with 100–200 tachyzoites per mouse three days before intraperitoneal injection.
Pru strain tachyzoites
T. gondii Pru strain chronically infected mice (intra-gastric inoculation with Pru cysts for more than 45 days) were euthanized and the brains were used for cysts separation. The brain homogenates were washed 2 times with Phosphate Buffered Saline (PBS). Lymphocytes separation medium (Sigma-Aldrich, 10771) was used to separate the lymphocyte from the cysts, and the cysts were collected from the bottom of the separation phases. The cysts were inoculated into peritoneal cavities of KM mice; the tachyzoites of Pru strain were then harvested from the ascites ten days post-infection.
Tachyzoites infection of cells
The harvested ascites were centrifuged for 5 min at room temperature at 3000 × g and quickly resuspended in DMEM complete medium. Cells transfected with plasmids or treated with siRNA for 48 h were infected with 1 × 105 T. gondii RH or Pru strain tachyzoites per well for 2 hr.
Transfection of plasmid DNA and short interference RNA (siRNA)
COS-7 cells were seeded in the 6-well plates and reached 70% confluence. Three μg of plasmid DNA per well were used for transfection with Lipofectamine™ LTX and plus reagent (invitrogen). Stealth double-stranded RhoA siRNA, and Rac1 siRNA and negative control (Neg Ctrl) siRNA were synthesized by Invitrogen (Carlsbad, CA, USA). SiRNA transfection was performed 24 hr after 16-HBE cells were seeded in the wells and reached 85% confluence. One hundred nmol of RhoA or Rac1 siRNA were used to silence RhoA or Rac1 separately and 100 nmol RhoA plus 100 nmol Rac1 siRNA were used for double silencing for transfection with Lipofectamine™ RNAi MAX (invitrogen). Mock transfection only contained transfection reagents.
Detection of the RNAi efficiency
The RNA interference (RNAi) efficiency was checked by Western-blot. The cells were harvested and lysed with RIPA lysis buffer (Thermo Scientific). One hundred μg of total proteins per well were loaded onto a SDS-PAGE gel and then transferred to a PVDF membrane for western blot detection.
GST pull down assay to detect the activation of RhoA and Rac1
16-HBE cells were cultured in six T-75 flasks to reach 100% confluency. Three flasks of cells were infected with T. gondii tachyzoites at a multiplicity of infection (MOI) of 10. The other three flasks of cells were kept as uninfected control (mock). At 3 hr post-infection, the medium from mock and infected flasks was aspirated and cells were trypsinized. Mock and infected cells were lysed in RIPA lysis buffer (Thermo Scientific) with ultrasonication. For negative control, 150 μg (600 μl) of the infected cell extract were aliquoted into two experimental tubes; 60 μl of loading buffer were added to each tube to a final concentration of 15 mM EDTA; 6 μl of GDP were added to these two tubes to a final concentration of 1.0 mM GDP and the tubes were incubated at room temperature for 15 min; the reaction was stopped by adding 60 μl of stopping buffer to each tube to a final concentration of 60 mM MgCl2.
The negative control cell lysate incubated with GDP, and 150 μg (600 μl) total protein from the lysate of infected, uninfected cells and T. gondii tachyzoites were added to 30 μg reconstituted GST-tagged Rhotekin-RBD protein on colored agarose beads for RhoA (Cytoskeleton Inc) or GST-tagged PAK-PBD protein bound colored agarose beads for Rac (Cytoskeleton Inc) respectively, and incubated at 4°C with rotating overnight. The beads were washed with PBS for 3 times. 25 μl protein loading buffer was added to each group of beads and boiled for 5 min then sediment at 12000 rpm for 1 min, the supernatant was used for SDS-PAGE. At the same time, 150 μg of total protein from the lysates of infected and uninfected cells and the T. gondii tachyzoites were used for SDS –PAGE, and actin in each group was detected via western-blot and used as the equal protein loading control for the GST pull down assay.
Primary antibodies: monoclonal rabbit anti-human RhoA antibody (Cell Signaling) and polyclonal rabbit anti-human Rac1 antibody (Abcam) were used in 1:1000 dilutions; β-actin was detected for loading control with monoclonal mouse anti-human anti-actin antibody (Cell Signaling) in 1:5000 dilutions. Secondary antibody: polyclonal sheep anti-mouse IgG-HRP antibody (Abcam) and polyclonal goat anti-rabbit IgG-HRP antibody (Abcam) were used in 1:3000 dilutions. ECL Western Blotting detection reagent was purchased from Pierce.
Immunofluorescence for endogenous RhoA and Rac1 after T. gondii infection
16-HBE cells were grown on coverslips to 80% confluence. The cells were infected with T. gondii RH tachyzoites. Two hr post-infection the unrecruited parasites were washed away with PBS. The cells were fixed with polyformaldehyde for 30 min and permeablized with Triton X-100 and blocked with 5% BSA in PBS. The cells were incubated with the primary antibody at 4°C overnight. The coverslips were washed 3 times with PBST, 5 min each wash, and then FITC conjugated secondary antibody was added to the coverslips and incubated for 1 hr at room temperature. The coverslips were washed 3 times with PBST, 5 min each wash, and stained with 10 nM DAPI (Sigma) for 10 min at room temperature, then washed three times with PBS (5 min each wash) with slight shaking. The coverslips were rinsed with double distilled water and air-dried. The coverslips were mounted and ready for fluorescence microscopy.
Primary antibodies of monoclonal rabbit anti-human RhoA antibody (Cell Signaling) and polyclonal rabbit anti- human Rac1 antibody (Abcam) were used in 1:100 dilutions. Secondary antibody of goat anti-Rabbit IgG-FITC (Abcam) was used in 1:500 dilutions.
Fluorescence microscopy for overexpressed CFP tagged RhoA and Rac1
COS-7 cells grown on the coverslips were transfected with the CFP-tagged RhoA and Rac1 plasmids for 48 hr, and then infected with tachyzoites for 2 hr. Washed three times with PBS, the cells were fixed in 4% polyformaldehyde for 30 min. After aspiration, cells were stained with 10 nM DAPI (Sigma) for 10 min at room temperature, then washed three times with PBS (5 min each wash) with slight shaking. The coverslips were rinsed with double distilled water, air dried and mounted for fluorescence microscopy.
Infection rate counting and statistical analysis
16-HBE cells, mock or transfected with Neg Ctrl siRNA, RhoA siRNA, Rac1 siRNA, and RhoA + Rac1 siRNA were infected with 1 × 105 T. gondii RH tachyzoites per well for 3 hr. The cells were washed 3 times with PBS. After aspiration, cells were stained with Giemsa stain (Sigma) for 10 min, and then washed three times with PBS (5 min each wash) with slight shaking. The coverslips were rinsed with double distilled water and air dried. The COS-7 cells overexpressing the CFP chimeras and the mock cells were also infected with 1 × 105 T. gondii RH tachyzoites per well for 2 hr. The COS-7 mock cells were stained with Giemsa stain as above mentioned. The CFP chimeras overexpressed in COS-7 cells do not need staining. The coverslips were rinsed with double distilled water and air dried.
The coverslips were mounted and ready for infection rate counting. For Giemsa stained cells, infection rate was the percentage of T. gondii tachyzoites infected cells among 100 cells randomly selected. In the CFP-tagged overexpressed group, the infection rate was presented as the percentage of those tachyzoites infected fluorescent cells among 100 fluorescent cells randomly selected. The infection rate experiment was performed in triplicate. The infection rates were divided by the mean infection rate of the mock group, and the percentages were used to compare the infection rate difference between different groups.
All data were shown as the mean ± S.E.M (Standard Error of Mean) for three separate experiments. The difference was analyzed based on One-way ANOVA and LSD test by SPSS software package. The statistical significance was defined as P < 0.01.
EGF activation of cytosol Rho GTPases in COS-7 cells and the translocalization observation
COS-7 cells transfected with pECFP-RhoA WT were starved overnight in DMEM medium without serum. On the second day, the cells were infected with RH tachyzoites for 2 hr. The media was aspirated after infection and cells were washed three times with PBS. For epidermal growth factor (EGF, Sigma, E9644 ) activation, 300 μl DMEM medium without serum was added to each well, 2 μl of 100 ng/μl EGF was added to one corner of the coverslips. The cells were fixed with paraformaldehyde 5 min after activation. The fixed cells were stained with DAPI for DNA visualization, and then washed 3 times with PBS (5 min each wash) with slight shaking. The coverslips were rinsed with double distilled water and air dried. At this point, coverslips were ready for the observation of RhoA GTPases translocalization.
Real-time observation of RhoA GTPase recruited to the PVM following T. gondiitachyzoites invasion
COS-7 cells were grown on 2 cm confocal plates and transfected with 3 μg pECFP-N1-Rho A WT when cells reached 70% confluency. Forty-eight hr later T. gondii RH tachyzoites were used to infect these COS-7 cells. The confocal plate was incubated in the tray (with 5% CO2 at 37°C) and connected to the confocal fluorescence microscope (Olympus FluoView® FV1000). The process of tachyzoites invading the host cell was visualized and pictures were taken automatically every 10 min.
Accumulation of Rho and Rac GTPases on the PVM
Real-time observation of recruitment of RhoA GTPase to the PVM
We found that the CFP-tagged RhoA was recruited to the PVM at the very beginning of the invasion, probably through retention of the RhoA GTPase on the host cell membrane to the PVM, and the accumulation of RhoA on the PVM continued with the recruitment of the tachyzoite until it totally invaded into the host cell (Figure 2A-D: pink arrowhead). However, a focal point of RhoA was not seen at the immediate point of invasion (Figure 2A). After the tachyzoite was completely inside the host cell, the fluorescence intensity continually increased with time, suggesting continuous accumulation of the RhoA GTPase on PVM probably by diffusion from the host cell cytosol (E-H, white and yellow arrowhead). The green fluorescence and the DIC images showing the invasion processes are provided in Additional file 1: Data S1 and Additional file 2: Data S2.
The recruitment of Rho A and Rac1 GTPases into PVM is dependent on the GTPase activity
The Rho A and Rac1 GTPases were activated upon T. gondiitachyzoite invasion
The recruitment of RhoA to T. gondiiPVM is dependent on different RhoA domains
The PVM-localized Rho and Rac GTPases do not respond to epithelial growth factor (EGF) activation
Interference with RhoA and Rac1 endogenous activity affects tachyzoite infection
The function of the Rho and Rac GTPases accumulated on PVM
Immunity-related GTPases (IRGs) also known as p47 GTPases, are key mediators of interferon-gamma-induced resistance to pathogens . They cycle between GDP-GTP bound forms, and cooperatively oligomerize in the GTP-bound conformation on the T. gondii PVM . Sequential recruitment of multiple IRGs to the PVM results in disruption of PVM and parasite digestion within 2 hr of infection . Virulent type I strains resist recruitment and avoid clearance, while less virulent type II and III strains are effectively cleared by IRGs . It was reported that a serine threonine kinase secreted by T. gondii, ROP18, binds to and phosphorylates IRGs on the PVM, and the phosphorylation of IRGs prevented clearance of T. gondii within inflammatory monocytes and IFN-γ-activated macrophages, conferring parasite survival in vivo. ARF6 is a small GTPase of the ARF family that regulates membrane trafficking and actin cytoskeleton rearrangements at the plasma membrane. ARF6 was found recruited to the PV of T. gondii tachyzoites and ARF6 activity was necessary for cell invasion by tachyzoites of T. gondii. These reports about the function of the GTPases on the PVM in T. gondii invasion urged us to hypothesize what is the function of the host cell Rho and Rac1 accumulating on the PVM.
Both the indirect immunofluorescence staining of the endogenous RhoA and Rac1 of the host cell, and the over-expressed CFP-tagged RhoA and Rac1 recombinant proteins in the host cell indicated the recruitment of RhoA and Rac1 in the PVM of T. gondii tachyzoites (Figure 1). From the real-time observation of the invasion of the host cell by T. gondii tachyzoites, we found that the recruitment of RhoA to the PVM happened at the very beginning of the invasion either from the membrane or from the cytosol (Figure 2). Those over-expressed CFP-tagged dominant negative mutants RhoA-N19 and Rac1-N17 did not accumulate to the PVM (Figure 3) implying the recruitment of RhoA and Rac1 is dependent on their GTPase activity. The GST-pull down assay detected greater amounts of GTP-bound RhoA and Rac1 in the infected host cells than in uninfected cells (Figure 4). Through CFP-tagged RhoA and Rac1 being visualized under the GFP filter, we found that RhoA and Rac1 GTPases in the host cell cytosol were translocated to the host cell membrane following EGF activation, while unlike the GTPases in the cytosol, RhoA or Rac1 on the PVM did not diffuse, translocate or respond to EGF activation. EGF activates RhoA and Rac1 through activation of the EGF pathway [24, 25]. This observation led us to hypothesize that the Rho and Rac1 GTPase recruited on the PVM probably was GTP-bound and could not be activated again by EGF, while most of the GTPases in the cytosol are in GDP-bound form and could be continually activated and translocated to the cell membrane upon EGF activation (Figure 6).
These observed results imply the invasion of the tachyzoites need the activation of RhoA and Rac1 GTPases; and the recruitment of these activated GTPases to the PVM is much more than a phenomenon as it may perform some as yet undefined but important function(s).
The decisive RhoA GTPases motifs for recruitment to parasitophorous vacuole membrane following T. gondiiinvasion
Wild-type Rho and Rac GTPases with normal GTPase activity were recruited to the PVM, but those mutants that constitutively bind only GDP (RhoA-N19 and Rac1-N17) lacked this ability. The 10 amino acid sequentially deleted RhoA mutants were used in the identification of the definitive motif(s) necessary for the recruitment to the PVM. M2 (RhoAΔ11–20), M3 (RhoAΔ21–30), M4 (RhoAΔ31–40), M7 (RhoAΔ61–70) and M17 (RhoAΔ161–170) lacked the ability to be recruited to the PVM (Figure 5). Based on the analysis of the RhoA GTPase conserved domains, the GTP/Mg2+ binding site, the mDia effector interaction site, the G1 box, the G2 box and the G5 box are the most potential motifs that determine the recruitment of RhoA GTPases to the PVM (Figure 5).
Rho GTPases are molecular switches that cycle between an active GTP-bound and an inactive GDP-bound form, which regulate many essential cellular processes, including actin dynamics, gene transcription, cell-cycle progression and cell adhesion . When in the active forms, Rho GTPases are able to interact with effector or target molecules to initiate downstream responses, signal transduction terminates when GTP is hydrolyzed to form GDP, and at which point the cycle is finished completely . The GTP/Mg2+ binding site of Rho GTPases is used to bind GTP and Mg2+, which activates the GTPases . The mDia effector interaction site is the domain that binds with mDia as a downstream Rho effector involved in microtubule stabilization. The mDia site induces stable microtubules that are capped and indicates that mDia may promote this microtubule capping by directly binding to microtubules. . The G1-G5 boxes are the GDP/GTP-binding motif elements that comprise a ~ 20 kDa phosphate domain (G domain, Ras residues 5–166), which is conserved in all Ras super family proteins . The decisive motifs are either related to GTP binding or with the effector regulating microtubules. This finding is consistent with our proposal that the recruitment of Rho GTPase to PVM depends on its enzymatic activity, and the invasion of T. gondii needs the rearrangement of host cell cytoskeleton.
Host cell RhoA and Rac1 activation is required for efficient cell invasion by T. gondiitachyzoites, which is a shared mechanism by many other intracellular pathogens infection
The major function of Rho GTPases activation is to regulate the dynamics and organization of the actin cytoskeleton , which is vital to the cell invasion of T. gondii tachyzoites. First, T. gondii tachyzoites invasion activates the reorganization of the microfilaments and microtubules of the host cell [31, 32]. Reorganization of host cell F-actin during entry of Toxoplasma tachyzoites has been visualized, and the entry was dependent on the actin dynamics . Second, any treatment to cease the normal cytoskeleton reorganization of host cells will impair T. gondii invasion efficiency. Cell invasion by T. gondii tachyzoites is significantly inhibited in cells treated with colchicum (a MT inhibitor) , cytochalasin D (an actin inhibitor) [14, 33] and jasplakinolide (a chemical disrupting actin filaments, which induces actin polymerization) . Maintenance of host cell actin cytoskeleton integrity is important to parasite invasion .
In our research, no significant difference was found in the infection rates of T. gondii tachyzoites in the cells overexpressed with RhoA or Rac1 wild-type proteins compared with those untransfected cells, while either overexpression of dominant negative mutants of RhoA or Rac1 or inhibition of endogenous RhoA or Rac1 in the host cell could significantly reduce cell invasion (Figure 7). This result indicated that it is the normal endogenous activity of RhoA and Rac1 that defines the efficiency of cell invasion by T. gondii tachyzoites, but not the amount of these proteins. This requirement is also reported in other intracellular pathogens. Shigella entry into HeLa cells induces membrane ruffling at the bacterial entry site, and the three Rho isoforms were recruited into bacterial entry sites. This membrane folding caused by invasion was abolished by using a Rho-specific inhibitor, and bacterial entry was impaired accordingly . Hela cells transfected with the dominant negative versions of Rac1 or RhoA reduced group B Streptococcus invasion by 75% and 51%, respectively, suggesting that Rho GTPases are indispensable for efficient invasion of HeLa cells by this bacterium . In MDCK cells, RhoA and Rac1were activated during Trypanosoma cruzi invasion and then triggered the reorganization of F-actin cytoskeleton, especially distinct in the invasion position on the cell membrane. The invasion of T. cruzi G strain extracellular amastigotes was specifically inhibited in Rac1-N17 dominant-negative cells [36, 37]. After the invasion of the rabbit corneal epithelial cells (SIRC) by Candida albicans, host cell actin filaments formed a rigid ring-like structure in the host cell. Immunochemical staining of actin and the expression of chimeric green fluorescent protein (GFP)-GTPases (RhoA, Rac1) showed the colocalization of the GTPases with actin at invasion and actin polymerization sites, but this colocalization was not seen in SIRC cells expressing a GFP-tagged dominant-negative mutant of GTPases. Inhibition of invasion was observed in SIRC cells expressing dominant-negative mutants of Rac1 and RhoA GTPases . These findings suggest that many pathogens may employ conserved pathways for invasion.
The Rho and Rac cell signaling involved in the cytoskeleton reorganization triggered by T. gondiiinvasion
RhoA and Rac1 GTPases from the host cell accumulated on the PVM after T. gondii invasion, and this accumulation was dependent on their GTPase activity and occurred regardless of the virulence of the parasitic strain. RhoA GTPase was recruited to the PVM as soon as the T. gondii tachyzoite invaded the host cell either through the host cell membrane or from the cytosol. Host cell RhoA and Rac1 were activated after T. gondii invasion. The decisive domains for the RhoA accumulation on the PVM were identified as the GTP/Mg2+ binding site, the mDia effector interaction site, the G1 box, the G2 box and the G5 box, respectively, which were related to the binding of GTP for enzymatic activity and to mDia for the regulation of microtubules. The reorganization of host cell cytoskeleton facilitates the PV formation and enlargement in the host cell. The recruited RhoA on the PVM could not be activated by epithelial growth factor (EGF) and no translocation was observed, which indicated that the recruited RhoA or Rac1 on the PVM might be in GTP-bound active form.
Wild-type RhoA or Rac1 overexpressed cells had almost the same infection rates by T. gondii as the mock-treated cells, while RhoA-N19 or Rac1-N17 transfected cells and RhoA or Rac1 siRNA- and RhoA + Rac1 siRNA-treated cells showed significantly diminished infection rates than mock cells, which indicated that the normal activity of RhoA and Rac1 GTPases are indispensable to the internalization of the tachyzoite. The accumulation of the RhoA and Rac1 on the PVM and the requisite of their normal GTPase activities for efficient invasion implied their involvement and function in T. gondii invasion. The summary of the host cell RhoA and Rac1 cell signaling involved in the T. gondii invasion is show in Figure 8.
Parasitophorous vacuole membrane
Epithelial growth factor
- Arf 6:
This work was supported by National Natural Science Foundation of China (No. 81071377, 81271866), the Research Fund for the Doctoral Program of Higher Education of China (20104433120014), Guangdong provincial key scientific and technological project to HJP (2011B010500003), Guangdong Province talent introduction of special funds (2011–26), the Guangdong Province College Students Renovation Experimental Program (1212111020) and the Grant from the School of Public Health and Tropical Medicine of Southern Medical University (GW201110) to HJ Peng; Province Universities and Colleges Pearl River Scholar Funded Scheme (2009) and National Natural Science Foundation of China (Key program:31030066) to XG Chen.
- Laliberte J, Carruthers VB: Host cell manipulation by the human pathogen Toxoplasma gondii. Cell Mol Life Sci. 2008, 65: 1900-1915. 10.1007/s00018-008-7556-x.PubMedPubMed CentralView Article
- Peng HJ, Chen XG, Lindsay DS: A review: Competence, compromise, and concomitance-reaction of the host cell to Toxoplasma gondii infection and development. J Parasitol. 2011, 97: 620-628. 10.1645/GE-2712.1.PubMedView Article
- Mordue DG, Hakansson S, Niesman I, Sibley LD: Toxoplasma gondii resides in a vacuole that avoids fusion with host cell endocytic and exocytic vesicular trafficking pathways. Exp Parasitol. 1999, 92: 87-99. 10.1006/expr.1999.4412.PubMedView Article
- Charron AJ, Sibley LD: Molecular partitioning during host cell penetration by Toxoplasma gondii. Traffic. 2004, 5: 855-867. 10.1111/j.1600-0854.2004.00228.x.PubMedView Article
- Mordue DG, Desai N, Dustin M, Sibley LD: Invasion by Toxoplasma gondii establishes a moving junction that selectively excludes host cell plasma membrane proteins on the basis of their membrane anchoring. J Exp Med. 1999, 190: 1783-1792. 10.1084/jem.190.12.1783.PubMedPubMed CentralView Article
- Hakansson S, Charron AJ, Sibley LD: Toxoplasma evacuoles: a two-step process of secretion and fusion forms the parasitophorous vacuole. EMBO J. 2001, 20: 3132-3144. 10.1093/emboj/20.12.3132.PubMedPubMed CentralView Article
- Joiner KA, Roos DS: Secretory traffic in the eukaryotic parasite Toxoplasma gondii: less is more. J Cell Biol. 2002, 157: 557-563. 10.1083/jcb.200112144.PubMedPubMed CentralView Article
- Straub KW, Cheng SJ, Sohn CS, Bradley PJ: Novel components of the Apicomplexan moving junction reveal conserved and coccidia-restricted elements. Cell Microbiol. 2009, 11: 590-603. 10.1111/j.1462-5822.2008.01276.x.PubMedPubMed CentralView Article
- Sibley LD, Charron A, Hakansson S, Mordue D: Invasion and intracellular survival by Toxoplasma gondii. Protozoans in Macrophages. Edited by: Denkers E, Gazzinelli R. 2007, Austin, TX: Landes Bioscience, 16-24.
- Scheffzek K, Ahmadian MR: GTPase activating proteins: structural and functional insights 18 years after discovery. Cell Mol Life Sci. 2005, 62: 3014-3038. 10.1007/s00018-005-5136-x.PubMedView Article
- Ghosh A, Uthaiah R, Howard J, Herrmann C, Wolf E: Crystal structure of IIGP1: a paradigm for interferon-inducible p47 resistance GTPases. Mol Cell. 2004, 15: 727-739. 10.1016/j.molcel.2004.07.017.PubMedView Article
- Takai Y, Sasaki T, Matozaki T: Small GTP-binding proteins. Physiol Rev. 2001, 81: 153-208.PubMed
- Hippenstiel S, Schmeck B, N’Guessan PD, Seybold J, Krüll M, Preissner K, Eichel-Streiber CV, Suttorp N: Rho protein inactivation induced apoptosis of cultured human endothelial cells. Am J Physiol Lung Cell Mol Physiol. 2002, 283: L830-838.PubMedView Article
- da Silva CV, da Silva EA, Cruz MC, Chavrier P, Mortara RA: ARF6, PI3-kinase and host cell actin cytoskeleton in Toxoplasma gondii cell invasion. Biochem Biophys Res Commun. 2009, 378: 656-661. 10.1016/j.bbrc.2008.11.108.PubMedView Article
- Howard JC, Hunn JP, Steinfeldt T: The IRG protein-based resistance mechanism in mice and its relation to virulence in Toxoplasma gondii. Curr Opin Microbiol. 2011, 14: 414-421. 10.1016/j.mib.2011.07.002.PubMedView Article
- Papic N, Hunn JP, Pawlowski N, Zerrahn J, Howard JC: Inactive and active states of the interferon-inducible resistance GTPase, Irga6, In Vivo. J Biol Chem. 2008, 283: 32143-32151. 10.1074/jbc.M804846200.PubMedPubMed CentralView Article
- Hall A: Rho GTPases and the actin cytoskeleton. Science. 1998, 279: 509-514. 10.1126/science.279.5350.509.PubMedView Article
- Maddala R, Reddy VN, Epstein DL, Rao V: Growth factor induced activation of Rho and Rac GTPases and actin cytoskeletal reorganization in human lens epithelial cells. Mol Vis. 2003, 17: 329-36.
- Taylor GA: IRG proteins: key mediators of interferon-regulated host resistance to intracellular pathogens. Cell Microbiol. 2007, 9: 1099-1107. 10.1111/j.1462-5822.2007.00916.x.PubMedView Article
- Hunn JP, Koenen-Waisman S, Papic N, Schroeder N, Pawlowski N, Lange R, Kaiser F, Zerrahn J, Martens S, Howard JC: Regulatory interactions between IRG resistance GTPases in the cellular response to Toxoplasma gondii. EMBO J. 2008, 27: 2495-2509. 10.1038/emboj.2008.176.PubMedPubMed CentralView Article
- Zhao YO, Khaminets A, Hunn JP, Howard JC: Disruption of the Toxoplasma gondii parasitophorous vacuole by IFN gamma-inducible immunity-related GTPases (IRG proteins) triggers necrotic cell death. PLoS Pathog. 2009, 5: e1000288-10.1371/journal.ppat.1000288.PubMedPubMed CentralView Article
- Zhao Y, Ferguson DJ, Wilson DC, Howard JC, Sibley LD, Yap GS: Virulent Toxoplasma gondii evade immunity-related GTPase-mediated parasite vacuole disruption within primed macrophages. J Immunol. 2009, 182: 3775-3781. 10.4049/jimmunol.0804190.PubMedPubMed CentralView Article
- Fentress SJ, Behnke MS, Dunay IR, Mashayekhi M, Rommereim LM, Fox BA, Bzik DJ, Taylor GA, Turk BE, Lichti CF, Townsend RR, Qiu W, Hui R, Beatty WL, Sibley LD: Phosphorylation of immunity-related GTPases by a Toxoplasma gondii-secreted kinase promotes macrophage survival and virulence. Cell Host Microbe. 2010, 8: 484-495. 10.1016/j.chom.2010.11.005.PubMedPubMed CentralView Article
- Yin J, Lu J, Yu FS: Role of small GTPase Rho in regulating corneal epithelial wound healing. Invest Ophthalmol Vis Sci. 2008, 49: 900-909. 10.1167/iovs.07-1122.PubMedPubMed CentralView Article
- Dise RS, Frey MR, Whitehead RH, Polk DB: Epidermal growth factor stimulates Rac activation through Src and phosphatidylinositol 3-kinase to promote colonic epithelial cell migration. Am J Physiol Gastrointest Liver Physiol. 2008, 294: G276-285.PubMedView Article
- Dvorsky R, Blumenstein L, Vetter IR, Ahmadian MR: Structural insights into the interaction of ROCKI with the switch regions of RhoA. J Biol Chem. 2004, 279: 7098-7104.PubMedView Article
- Bishop AL, Hall A: Rho GTPases and their effector proteins. Biochem J. 2000, 348: 241-255. 10.1042/0264-6021:3480241.PubMedPubMed CentralView Article
- Ihara K, Muraguchi S, Kato M, Shimizu T, Shirakawa M, Kuroda S, Kaibuchi K, Hakoshima T: Crystal structure of human RhoA in a dominantly active form complexed with a GTP analogue. J Biol Chem. 1998, 273: 9656-9666. 10.1074/jbc.273.16.9656.PubMedView Article
- Palazzo AF, Cook TA, Alberts AS, Gundersen GG: mDia mediates Rho-regulated formation and orientation of stable microtubules. Nat Cell Biol. 2001, 8: 723-729.View Article
- Wennerberg K, Rossman KL, Der CJ: The Ras superfamily at a glance. J Cell Sci. 2005, 118: 843-846. 10.1242/jcs.01660.PubMedView Article
- Gonzalez V, Combe A, David V, Malmquist NA, Delorme V, Leroy C, Blazquez S, Ménard R, Tardieux I: Host cell entry by apicomplexa parasites requires actin polymerization in the host cell. Cell Host Microbe. 2009, 5: 259-272. 10.1016/j.chom.2009.01.011.PubMedView Article
- Walker ME, Hjort EE, Smith SS, Tripathi A, Hornick JE, Hinchcliffe EH, Archer W, Hager KM: Toxoplasma gondii actively remodels the microtubule network in host cells. Microbes Infect. 2008, 10: 1440-1449. 10.1016/j.micinf.2008.08.014.PubMedPubMed CentralView Article
- Li L, Li X, Yan J: Alterations of concentrations of calcium and arachidonic acid and agglutinations of microfilaments in host cells during Toxoplasma gondii invasion. Vet Parasitol. 2008, 157: 21-33. 10.1016/j.vetpar.2008.07.007.PubMedView Article
- Adam T, Giry M, Boquet P, Sansonetti P: Rho-dependent membrane folding causes Shigella entry into epithelial cells. EMBO J. 1996, 15: 3315-3321.PubMedPubMed Central
- Burnham CA, Shokoples SE, Tyrrell GJ: Rac1, RhoA, and Cdc42 participate in HeLa cell invasion by group B streptococcus. FEMS Microbiol Lett. 2007, 272: 8-14. 10.1111/j.1574-6968.2007.00768.x.PubMedView Article
- Fernandes AB, Mortara RA: Invasion of MDCK epithelial cells with altered expression of Rho GTPases by Trypanosoma cruzi amastigotes and metacyclic trypomastigotes of strains from the two major phylogenetic lineages. Microbes Infect. 2004, 6: 460-467. 10.1016/j.micinf.2004.01.009.PubMedView Article
- Bonilha VL, De Souza W, Carvalho TM: Role of small GTPases in Trypanosoma cruzi invasion in MDCK cell lines.Dutra JM. Parasitol Res. 2005, 96: 171-177. 10.1007/s00436-005-1333-7.PubMedView Article
- Atre AN, Surve SV, Shouche YS, Joseph J, Patole MS, Deopurkar RL: Association of small Rho GTPases and actin ring formation in epithelial cells during the invasion by Candida albicans. FEMS Immunol Med Microbiol. 2009, 55: 74-84. 10.1111/j.1574-695X.2008.00504.x.PubMedView Article
- Kraus S, Benard O, Naor Z, Seger R: c-Src is activated by the epidermal growth factor receptor in a pathway that mediates JNK and ERK activation by gonadotropin-releasing hormone in COS7 cells. J Biol Chem. 2003, 35: 32618-32630.View Article
- Cowan CW, Shao YR, Sahin M, Shamah SM, Lin MZ, Greer PL, Gao S, Griffith EC, Brugge JS, Greenberg ME: Vav family GEFs link activated Ephs to endocytosis and axon guidance. Neuron. 2005, 2: 205-217.View Article
- Tanaka M, Ohashi R, Nakamura R, Shinmura K, Kamo T, Sakai R, Sugimura H: Tiam1 mediates neurite outgrowth induced by ephrin-B1 and EphA2. EMBO J. 2004, 5: 1075-1088.View Article
- Knoll B, Drescher U: Src family kinases are involved in EphA receptor-mediated retinal axon guidance. J Neurosci. 2004, 28: 6248-6257.View Article
- Sahin M, Greer PL, Lin MZ, Poucher H, Eberhart J, Schmidt S, Wright TM, Shamah SM, O’connell S, Cowan CW, Hu L, Goldberg JL, Debant A, Corfas G, Krull CE, Greenberg ME: Eph-dependent tyrosine phosphorylation of ephexin1 modulates growth cone collapse. Neuron. 2005, 2: 191-204.View Article
- Sumi T, Matsumoto K, Nakamura T: Specific activation of LIM kinase 2 via phosphorylation of threonine 505 by ROCK, a Rho-dependent protein kinase. J Biol Chem. 2001, 1: 670-676.View Article
- Arimura N, Inagaki N, Chihara K, Menager C, Nakamura N, Amano M, Iwamatsu A, Goshima Y, Kaibuchi K: Phosphorylation of collapsin response mediator protein-2 by Rho-kinase. Evidence for two separate signaling pathways for growth cone collapse. J Biol Chem. 2000, 31: 23973-23980.View Article
- Fukata Y, Itoh TJ, Kimura T, Menager C, Nishimura T, Shiromizu T, Watanabe H, Inagaki N, Iwamatsu A, Hotani H, Kaibuchi K: CRMP-2 binds to tubulin heterodimers to promote microtubule assembly. Nat Cell Biol. 2002, 8: 583-591.
- Liu BP, Burridge K: Vav2 activates Rac1, Cdc42, and RhoA downstream from growth factor receptors but not β1 integrins. Mol Cell Biol. 2000, 20: 7160-7169. 10.1128/MCB.20.19.7160-7169.2000.PubMedPubMed CentralView Article
- Wilson JG: Reproduction and teratogenesis: current methods and suggested improvements. J Assoc Off Anal Chem. 1975, 4: 657-667.
- Maekawa M, Ishizaki T, Boku S, Watanabe N, Fujita A, Iwamatsu A, Obinata T, Ohashi K, Mizuno K, Narumiya S: Signaling from Rho to the actin cytoskeleton through protein kinases ROCK and LIM-kinase. Science. 1999, 5429: 895-898.View Article
- Dayel MJ, Mullins RD: Activation of Arp2/3 complex: addition of the first subunit of the new filament by a WASP protein triggers rapid ATP hydrolysis on Arp2. PLoS Biol. 2004, 4: E91-View Article
- Fan L, Di Ciano-Oliveira C, Weed SA, Craig AW, Greer PA, Rotstein OD, Kapus A: Actin depolymerization-induced tyrosine phosphorylation of cortactin: the role of Fer kinase. Biochem J. 2004, 2: 581-591.View Article
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