Identification of a novel lipin homologue from the parasitic protozoan Trypanosoma brucei
© Pelletier et al.; licensee BioMed Central Ltd. 2013
Received: 3 December 2012
Accepted: 6 May 2013
Published: 8 May 2013
Arginine methylation is a post-translational modification that expands the functional diversity of proteins. Kinetoplastid parasites contain a relatively large group of protein arginine methyltransferases (PRMTs) compared to other single celled eukaryotes. Several T. brucei proteins have been shown to serve as TbPRMT substrates in vitro, and a great number of proteins likely to undergo methylation are predicted by the T. brucei genome. This indicates that a large number of proteins whose functions are modulated by arginine methylation await discovery in trypanosomes. Here, we employed a yeast two-hybrid screen using as bait the major T. brucei type I PRMT, TbPRMT1, to identify potential substrates of this enzyme.
We identified a protein containing N-LIP and C-LIP domains that we term TbLpn. These domains are usually present in a family of proteins known as lipins, and involved in phospholipid biosynthesis and gene regulation. Far western and co-immunoprecipitation assays confirmed the TbPRMT1-TbLpn interaction. We also demonstrated that TbLpn is localized mainly to the cytosol, and is methylated in vivo. In addition, we showed that, similar to mammalian and yeast proteins with N-LIP and C-LIP domains, recombinant TbLpn exhibits phosphatidic acid phosphatase activity, and that two conserved aspartic acid residues present in the C-LIP domain are critical for its enzymatic activity.
This study reports the characterization of a novel trypanosome protein and provides insight into its enzymatic activity and function in phospholipid biosynthesis. It also indicates that TbLpn functions may be modulated by arginine methylation.
KeywordsKinetoplastid Lipin Arginine methylation Phosphatidic acid phosphatase
Arginine methylation is a post-translational modification whose importance and widespread impact has recently begun to be fully appreciated [1–4]. In yeast and mammals, arginine methylation has been associated with a diversity of cellular processes including signal transduction [5, 6], RNA transport [7, 8] and processing [9–12], protein localization [13–15], and transcription . The effects of arginine methylation on these processes are exerted primarily through the modulation of protein-protein and, less often, protein-nucleic acid interactions [17–20]. Common sites of arginine methylation within proteins include RGG, RG, or RXR motifs [21–23], although methylation of arginine also occurs within other sequence contexts . Catalysis of arginine methylation is carried out by a family of enzymes termed protein arginine methyltransferases [PRMTs). While these enzymes are apparently absent from prokaryotes, putative PRMTs have been identified in the genomes of all eukaryotes examined with the exception of Giardia lamblia[1, 25, 26].
PRMTs are classified into four types. Both type I and II PRMTs catalyze the formation of ω-NG monomethylarginine (MMA). Type I enzymes subsequently synthesize ω-NG,NG asymmetrical dimethylarginine (ADMA), while the type II enzymes form ω-NG,N’G symmetrical dimethylarginine (SDMA). Type III and type IV enzymes catalyze the formation of only ω-NG monomethylarginine (MMA) or δ-NG monomethylarginine, respectively. In humans, nine PRMTs have been confirmed, most of them being type I enzymes . In contrast to what has been described in humans, only three PRMTs have been described in Saccharomyces cerevisiae, one each of type I type II, and the apparently fungal-specific type IV . Most protozoa with the exception of Giardia who lacks putative PTMTS, are predicted to possess at least one type I and one type II PRMTs .
Trypanosoma brucei is a parasitic protozoan and the causative agent of African sleeping sickness in humans and nagana in African livestock. The genome of T. brucei predicts the presence of five PRMTs , a relatively large number for a single celled organism . These PRMTS, with the exception of the putative type I TbPRMT3, have previously been characterized. TbPRMT1 is the major type I PRMT in T. brucei, analogous to its role in yeast and mammals . TbPRMT5 is a type II enzyme homologous to human PRMT5 . TbPRMT7 is a novel, kinetoplastid-specific type III PRMT . Finally, the recently characterized TbPRMT6 is a type I PRMT capable of automethylation . To date, only a few arginine methylproteins have been reported in T. brucei. These include the mitochondrial RNA binding proteins RBP16, TbRGG1, TbRGG2, and MRP2. The effects of RBP16 methylation have been characterized. RBP16 is a TbPRMT1 substrate, as shown by in vitro methylation assays and the hypomethylated state of RBP16 in TbPRMT1 knockdown cells . Arginine methylation affects the ability of RBP16 to stabilize specific mitochondrial RNAs and exerts both positive and negative impacts on the interaction of RBP16 with different classes of RNAs and ribonucleoprotein complexes [18, 31]. In addition, a large number of proteins harboring arginine/glycine rich regions likely to undergo methylation are predicted by the T. brucei genome, and several T. brucei RNA binding proteins serve as TbPRMT substrates in vitro[26–29, 32]. This indicates that a large number of proteins whose functions are modulated by arginine methylation await discovery in trypanosomes.
To gain insight into functions of arginine methylation in trypanosome gene regulation, we set out to identify substrates of the major T. brucei type I PRMT, TbPRMT1. We performed a yeast two-hybrid screen using the entire TbPRMT1 open reading frame as bait, exploiting the propensity of PRMTs to associate in a relatively stable manner with their substrates . Using this approach, we identified a protein containing two conserved domains found in a family of proteins known as lipins. Lipins are involved in adipocyte development and phospholipid biosynthesis in mammalian and yeast cells. We termed this protein TbLpn. While these two domains, known as N-LIP and C-LIP domains, are found in several mammalian and yeast lipin proteins [34, 35], TbLpn possesses no homology to known proteins outside these two domains and is, thus, a kinetoplastid-specific protein. Consistent with the yeast-two-hybrid data, we show that TbLpn interacts in vivo with TbPRMT1, and that it is methylated on arginine residues in vivo. We also show that, as predicted by the presence of conserved domains, TbLpn displays phosphatidic acid phosphatase activity in vitro, and that the two conserved aspartic acid residues present in the C-LIP domain, are essential for enzymatic activity.
Identification of TbLpn as a TbPRMT1-interacting protein
To begin to understand the functions of protein arginine methylation in trypanosomes, we sought to identify proteins that interact with the major type I PRMT in T. brucei, TbPRMT1. PRMTs tend to associate in a relatively stable manner with their substrates, and several mammalian methylproteins have been identified through protein-protein interaction screens with PRMTs [36, 37]. To identify TbPRMT1-interacting proteins, we screened a yeast-two-hybrid library comprised of mixed procyclic (PF) and bloodstream form (BF) T. brucei cDNA  using the entire TbPRMT1 ORF as bait. Approximately 800 colonies that grew under moderate selection on SD medium (-Trp, -Leu, -His) were selected for more stringent screening on SD medium (-Trp, -Leu, -His, -Ade).
Subcellular localization of TbLpn
TbLpn interacts with TbPRMT1 in vitro and in vivo
In order to examine the interaction between TbPRMT1 and TbLpn in vivo, we performed a co-immunoprecipitation. As shown above, TbLpn is located in the cytosol of the parasite. For this reason, TbLpn was immunoprecipitated from PF T. brucei cytosolic extracts using purified polyclonal anti-TbLpn antibodies. Proteins that were immunoprecipitated along with TbLpn were separated by electrophoresis and transferred onto PVDF. The presence of TPRMT1 in association with TbLpn was determined by using purified polyclonal anti-TbPRMT1 antibodies to probe the membrane by western hybridization. The results shown in Figure 4B clearly show that a band of approximately the size of TbPRMT1 (38.9 kDa) co-precipitates exclusively with TbLpn, and is not present in the negative control.
TbLpn is methylated in vivo
TbLpn displays phosphatidic acid phosphatase activity in vitro
In an effort to discover trypanosome substrates of PRMTs, we utilized a yeast two-hybrid screen to identify proteins that associate with the major type I protein arginine methyltransferase in T. brucei, TbPRMT1 . Of particular interest to us are proteins whose functions might be affected by arginine methylation. Here, we report that TbPRMT1 directly interacts in both Far Western and co-immunoprecipitation assays with a novel protein. We termed this protein TbLpn, based on the presence of two conserved (N-LIP and C-LIP) domains found in a family of proteins called lipins. We further demonstrate that, like TbPRMT1, TbLpn is cytoplasmic in PF T. brucei, consistent with a function in TbLpn methylation. Together, these data point to TbLpn as a candidate protein whose post-transcriptional gene regulatory functions are affected by arginine methylation.
We demonstrated that, as predicted from the amino acid sequence, recombinant TbLpn, as other members of the lipin family, exhibits phosphatidic acid phosphatase enzymatic activity. Mutation of the conserved aspartic acid residues (Asp-445 and Asp- 447) to alanines results in a significant reduction in the enzymatic activity of TbLpn. These two aspartic acid residues are part of the conserved DxDxT motif found in lipin proteins and other members of the haloacid dehalogenase (HAD)-like superfamily [53, 54]. Based on the crystal structure of L-2-haloacid dehalogenase from Pseudomonas, it is likely that Asp-445 in TbLpn acts as a nucleophile in the phosphoryl transfer reaction.
Compared to the recombinant yeast PAH1 (3000 nmol/min/mg) and human Lipin-1 (1,600 nmol/min/mg), His ~ TbLpn displays a lower but still significant specific activity . One possible explanation for this lower specific activity is the fact that the recombinant protein may not contain the same post-translational modifications as those found in the native protein. It is of interest that several lipin homologues are highly modified at the post translational level. In rat and in mouse adipocytes, Lipin 1 contains at least 19 and as many as 23 sites that are phosphorylated in response to insulin [49, 55, 56]. Although it does not affect its intrinsic phosphatidic acid phosphatase activity, phosphorylation of Lipin-1 decreases the association with intracellular membranes, thus the active lipin fraction . In addition, the lipin homologue SMP2 is phosphorylated by the cyclin-dependent kinase Cdc28/Cdk1 in budding yeast . The authors have shown that phosphorylation of SMP2 by Cdc28/Cdk1 enhances its association with promoters of lipid biosynthetic genes, which leads to their transcriptional down-regulation. Careful analysis of TbLpn amino acid sequence revealed the presence of 5 conserved amino acid residues shown to be phosphorylated in either mouse (Mm) Lipin-1 or yeast (Sc) Smp2. These residues are Ser-102 (Ser-110 in Sc), Thr-239 (Thr-282 in Mm), Thr-255 (Thr-298 in Mm), Ser-282 (Ser-328 in Mm), and Ser-343 (Ser-392 in Mm). In addition, a previous analysis of the cytosolic phosphoproteome of BF T. brucei identified TbLpn as containing two phosphorylated residues (Thr-211 and Ser-221) . Whether additional or different amino acids are phosphorylated in the PF is still unclear.
Phosphorylation of TbLpn may also impact its association with other proteins, as it has been demonstrated for at least one other member of the lipin family. In adipocytes, Lipin-1 interacts directly with 14-3-3 proteins . 14-3-3- proteins are known to regulate the subcellular localization of a wide variety of proteins through interaction with phosphoserine residues. In adipocytes, Lipin-1 is mostly localized to the cytosol and translocate to the endoplasmic reticulum membrane where it catalyzes dephosphorylation of phosphatidic acid. Stimulation of adipocytes by insulin promotes phosphorylation of Lipin-1 and enhances binding by 14-3-3 proteins. This results in cytoplasmic retention of Lipin-1.
Additionally, we showed that TbLpn is methylated on arginine residues in vivo. To our knowledge, this is the first instance of any lipin or phosphatidic acid phosphatase being methylated. The demonstration that TbLpn is methylated in vivo suggests that methylation could directly modulate TbLpn enzymatic activity or protein-protein interactions, or both. Arginine methylation has been shown to generally alter protein function by modulating protein-protein interactions, protein-nucleic acid interactions, and protein trafficking [11, 21, 59, 60]. Arginine residues that serve as substrates for PRMTs are usually found within glycine/arginine rich (GAR) domains [61–63]. Based on this, arginine residues throughout TbLpn, including both the N-LIP and C-LIP domains are predicted to undergo methylation. Thus, it will be of great future interest to determine whether TbPRMT1 and/or other TbPRMTs are responsible for TbLpn methylation in vivo, and to determine whether TbLpn methylation has any effect on its ability to interact with other proteins and whether it modulates its enzymatic activity.
In yeast and mammals, lipin proteins enable the cell to generate diacylglycerol (DAG) by catalyzing the dephosphorylation of phosphatidic acid. In addition to serving as a precursor for triacylglycerol (TAG), DAG is also used to synthesize phosphatidylcholine (PC) and phosphatidylethanolamine (PE) . In mammalian and yeast cells, the bulk of the PC pool is synthesized by the CDP-choline branch of the Kennedy pathway . In addition, a small fraction of PC is generated by sequential methylation of PE . In eukaryotes, PE can be synthesized by decarboxylation of phosphatidylserine (PS), by head group exchange with PS, by acylation of lyso-PE, or by the CDP-ethanolamine branch of the Kennedy pathway [65, 66]. As for other eukaryotes, PC and PE constitute the majority of phospholipids in trypanosomes . Of great importance is the fact that, as opposed to other parasitic organisms, trypanosomes synthesize phospholipids de novo. Although the pathways for phospholipids biosynthesis have not been very well characterized, recent data have helped to better understand how trypanosomes are able to assemble phospholipids. In T. brucei, PC is synthesized solely by the CDP-choline branch of the Kennedy pathway, while PE is produced exclusively via the CDP-ethanolamine branch of the Kennedy pathway [67, 69, 70]. Disruption of the enzymes of the CDP-ethanolamine pathway by RNA interference have shown that this branch of the Kennedy pathway is essential for both procyclic and bloodstream form T. brucei cell growth [69, 71].
PE and phosphatidylinositol (PI) are key phospholipids involved in the biosynthesis of glycosylphosphatidylinositol (GPI). In trypanosomes, a large number of surface proteins with critical role in virulence surface proteins are anchored to the plasma membrane via GPI molecules. One of these proteins is the variant surface glycoprotein (VSG), a major virulence factor that undergoes antigenic variation and enables the parasite to evade the immune system of its mammalian host . The steps involved in the biosynthesis of GPI, a process essential for T. brucei bloodstream form survival, have been well studied. This synthesis differs in certain aspects from the pathway in mammalian cells and yeast. In T. brucei, the pool of PI used for GPI synthesis is supplied from glucose-6-phosphate by the action of PI synthase, an enzyme shown to be essential in both bloodstream and procyclic form trypanosomes [68, 70, 71]. A crucial step in the GPI synthesis pathway is the transfer of phosphoethanolamine (PEtN) to mannose residues on the growing GPI. In this reaction, the ethanolamine moiety is provided by PE . As described earlier, synthesis of PE in T. brucei is carried out via the CDP-ethanolamine branch of the Kennedy pathway using DAG as the initial substrate. It has been demonstrated that inhibition of PE synthesis prevents de novo GPI biosynthesis . As we demonstrated in the current paper that TbLpn catalyzes the dephosphorylation of PA into DAG, it is attractive to speculate that TbLpn plays an important role in GPI biosynthesis, and thus in the expression of this major virulence factor.
The results clearly identify TbLpn as a new member of the lipin family of proteins. The presence of the conserved N-LIP and C-LIP domains, and especially the ability of recombinant TbLpn to dephosphorylate phosphatidic acid indicate that this enzyme is likely to be involved in phospholipid biosynthesis in trypanosomes. Finally, the observation that, in vivo, TbLpn contains methylated arginine residues is very significant, as it is the only lipin or phosphatidic acid phosphatase to date to exhibit such a post-translational modification.
Procyclic form T. brucei brucei clone IsTaR1 stock EATRO 164 was grown as described in SDM-79 medium supplemented with 15% fetal bovine serum (FBS) .
Identification of TbLpn by yeast two-hybrid screening
For two-hybrid screening, the TbPRMT1 open reading frame (ORF) was amplified by 35 cycles of PCR from pMal-TbPRMT1  using primers PRMT1-Y2H/myc-5′ (5′-GCTCTAGACATATGACGGTGGACGCAAATGCCG-3′) and PRMT-Y2H/myc-3′ (5′-GCGGATCCCTATCTAGACCGCAGCCGAAAATCCTGGTC-3′) which allowed introduction of NdeI and BamHI restriction sites respectively (underlined). The PCR product was then cloned into NdeI and BamHI sites of pAS2-1 (CLONTECH Laboratories), and transformed into Escherichia coli DH5α competent cells (Invitrogen).
The bait plasmid pAS2-TbPRMT1 was co-transformed into the competent yeast strain AH109, along with a mixed procyclic and bloodstream form T. brucei cDNA library (a generous gift from George Cross, Rockefeller Univ. and Vivian Bellofatto, UMDNJ) cloned into pGADT7 (CLONTECH Laboratories) using the LiAc/PEG method . Transformed cells were plated onto synthetic dextrose medium (SD) supplemented with an amino acid dropout solution lacking histidine (His), leucine (Leu), and tryptophan (Trp) and incubated at 30°C. Resultant colonies were then streaked onto SD medium lacking His, Leu, Trp, and adenine (Ade). Colonies that grew on this medium were grown overnight at 30°C in 3 ml of SD broth lacking Leu. Cells were collected by centrifugation at 14,000 × rpm for 5 min in a Biofuge centrifuge. The pellet was resuspended in about 50 μl of residual liquid, and 10 μl of a 10 units/μl lyticase solution was added and thoroughly mixed. Cell lysis was allowed to proceed at 37°C for 60 min with shaking at 250 rpm. Twenty μl of 10% SDS was then added and the tube vortexed for 1 min. The samples were then put to a freeze/thaw cycle (at -20°C) and vortexed one more time. The plasmid was purified using a GFX DNA purification column (GE Healthcare) following the manufacturer’s instructions, and eluted with 50 μl of deionized water. Five μl of the purified plasmid was used to transform 20 μl of ELECTROMAX DH10B cells (Invitrogen). Briefly, electroporation was carried out on ice in 2-mm cuvettes using a Bio-Rad electroporator with the following settings: 2,000 V, 25 μF, 200 Ω. Following electroporation, 1 ml of SOC was added and the cells were transferred to a 15-ml snap cap tube, and incubated for 60 min at 37°C with shaking (250 rpm). Fifty and 500 μl were then plated onto LB plates containing 0.1 mg/ml ampicillin, and cells were allowed to grow at least 18 hours at 37°C. Colonies with pGADT7 containing a DNA fragment were identified by PCR using primers GAL4AD5′ (5′-CAGGGATGTTTAATACCACTA-3′) and GAL4AD3′ (5′-GCACAGTTGAAGTGAACTTGC-3′), and sequenced.
Production of recombinant TbLpn
C-terminally his-tagged TbLpn was generated as follows. Total PF cDNA was generated by reverse transcription primed with [dT]-RXS. The entire TbLpn ORF was amplified using Deep Vent DNA polymerase (New England Biolabs), and using oligonucleotides his10-lipin-5′ (5′-CGG GATCCATGATATCTGGTTTTGCAGATTTC-3′) and his10-lipin3′ (5′-CCCAAGCTTCCGCTCGAGTCACACAGTGTCACCTTGTTGATA-3′) (restriction sites are underlined) which were constructed based on the genomic sequence. The PCR product was then digested with BamHI and XhoI, ligated into the pET26-His10Smt3 expression vector, giving rise to pHis10-TbLpn, and transformed into E. coli BL21 competent cells (Invitrogen). A mutant version of TbLpn, in which the two conserved aspartic acid residues in the DVDGT motif (Asp-445, Asp-447) are changed to alanine (pHis-TbLpn(DEAD)), was generated by PCR amplification from pHis10-TbLpn using the QuikChange II XL™ Site-Directed Mutagenesis Kit (Agilent Technologies) and the mutagenic primers TbLpn-DEAD-5′ (5′-CTTGTCATTAGTGAAGTGGAAGGCACGATCACGAAAAG-3′) and TbLpn-DEAD-3′ (5′-CTTTTCGTGATCGTGCCTTCCACTTCACTAATGACAAG-3′).
Protein expression was induced with 1 mM isopropyl β-thiogalactopyranoside (IPTG) and 2% ethanol for 20 h at 17°C. Cells were resuspended in lysis buffer (10 mM Tris [pH 8.6], 10 mM glycine, 300 mM NaCl, 10 mM imidazole, 10% glycerol, 10% ethanol, 4% Tween-20, and 3% Triton X-100) containing 0.05 mg/ml lysozyme, 0.01 mg/ml DNase I, 1 mM phenylmethylsulfonyl fluoride (PMSF), 1 μg/ml leupeptin, and 1μg/ml pestatin A, and lysed by 3 freeze/thaw cycles. Each cycle consisted of incubation at 37°C for 15 minutes, followed by incubation at -80°C for another 15 minutes. The lysed cell suspension was centrifuged at 17,000 × g for 15 min at 4°C, and the supernatant was mixed with Probond Ni2+ resin (Invitrogen) for 12 h at 4°C. The mixture was poured into a column and the column washed with 40 volumes of wash buffer (10 mM Tris [pH 7.0], 200 mM NaCl, 30 mM imidazole, 10% glycerol). His-tagged proteins were eluted with 10 volumes of wash buffer (pH 6.0) containing 200 mM imidazole.
Polyclonal antibody production
Affinity purified polyclonal anti-TbLpn antibodies were obtained from Bethyl Laboratories, Inc. using a peptide corresponding to amino acids 791–806 (GLCNTSSENYQQGDTV).
Far western analysis
His-tagged TbLpn was electrophoresed on a denaturing 10% SDS-polyacrylamide gel and transferred onto a polyvinylidene fluoride (PVDF) membrane at 50 V for 45 min in 10 mM 3-[Cyclohexylamino]-1-propanesulfonic acid (CAPS) buffer (pH 11.0) containing 10% methanol. As a negative control, his-tagged RBP16 was expressed as described  and purified using the same protocol used for the purification of His-TbLpn described above. The membrane was blocked in TBS buffer containing 5% nonfat dry milk for 1 hour, washed twice for 5 min in TBS buffer containing 0.05% Tween-20 (TBS-T), and then incubated with 0.5-1.0 μg of purified TbPRMT1  in TBS-T containing 2% nonfat dry milk overnight at 4°C. After two 15 minute washes in TBS-T, the membrane was probed with anti-TBPRMT1 polyclonal antibodies (1:1,000) for 2 hours, washed in TBS-T twice for 15 min, and incubated with goat anti-rabbit IgGs coupled to horseradish peroxidase. Reactive proteins were detected using enhanced chemiluminescence (GE Healthcare).
Preparation and fractionation of trypanosome cellular extracts
Log-phase PF T. brucei brucei clone IsTaR1 stock EATRO 164 were harvested by centrifugation at 6,090 × g for 10 min at 4°C. Fractionation of trypanosome cellular extracts was performed as described previously . The integrity of the cellular compartment was confirmed by using antibodies directed against the cytosolic protein Hsp70 or the nuclear RNA polymerase II .
Immunoprecipitation of TbLpn from T. bruceicytosolic extracts
As it was previously determined that TbLpn is localized in the cytosol, immunoprecipitation of TbLpn was performed using PF form T. brucei cytosolic extracts. Ten μg of purified anti-TbLpn antibodies or 10 μl of IP buffer (for mock immunoprecipitations) (20 mM Hepes [pH 7.9], 150 mM sucrose, 150 mM KCl, 3 mM MgCl2, 0.5% Nonidet- P40, 1 μg/ml of pestatin A, 1 μg/ml of leupeptin, 5 mM PMSF) were added to 200 μl of cytosolic extract in a final volume of 300 μl of IP buffer. The samples were incubated at 4°C for at least 12 h with gentle rotation. Ten μl of Protein A-Sepharose (GE Healthcare) was then added, and the samples incubated 1 hour at 4°C with gentle rotation. Immune complexes were recovered by centrifugation at 3,000 × g for 30 s and washed five times, each time for 5 min, with 1 ml of IP buffer.
Phosphatidic acid phosphatase assays
The standard reaction contained 50 mM Tris–HCl buffer (pH 7.5), 1 mM MgCl2, and 0.4 mM 1,2-dioctanoyl-sn-glycero-3-phosphate (DiC8 PA) (Avanti Polar Lipids) in a total volume of 50 μl. Reactions were initiated by the addition of recombinant proteins (50–250 ng), and carried out in triplicate at 30°C for 30 min. The reaction was terminated by the addition of 100 μl of PiBlue reagent (BioAssay Systems), and the color allowed to develop at room temperature for 30 minute. The absorbance was measured with a spectrophotometer at 620 nm. The amount of phosphate produced was quantified from a standard curve using 0.5–4 nmol of potassium phosphate. The reactions were linear with time and protein concentration. The enzymatic activity was expressed as the number of pmol of phosphate released per minute.
We thank Dr. Laurie K. Read (University at Buffalo, Department of Microbiology and Immunology) for providing several reagents essential to the completion of many experiments. We are also grateful to Dr. Adam Rich (The College at Brockport, Department of Biology) for helpful discussions.
- Bachand F: Protein arginine methyltransferases: from unicellular eukaryotes to humans. Eukaryot Cell. 2007, 6: 889-898. 10.1128/EC.00099-07.PubMedPubMed CentralView ArticleGoogle Scholar
- Bedford MT: Arginine methylation at a glance. J Cell Sci. 2007, 120: 4243-4246. 10.1242/jcs.019885.PubMedView ArticleGoogle Scholar
- Bedford MT, Clarke SG: Protein arginine methylation in mammals: who, what, and why. Mol Cell. 2009, 33: 1-13. 10.1016/j.molcel.2008.12.013.PubMedPubMed CentralView ArticleGoogle Scholar
- Krause CD, Yang ZH, Kim YS, Lee JH, Cook JR, Pestka S: Protein arginine methyltransferases: evolution and assessment of their pharmacological and therapeutic potential. Pharmacol Ther. 2007, 113: 50-87. 10.1016/j.pharmthera.2006.06.007.PubMedView ArticleGoogle Scholar
- Boisvert FM, Chénard CA, Richard S: Protein interfaces in signaling regulated by arginine methylation. Sci Signal. 2005, 271: re2-Google Scholar
- Weber S, Maaβ F, Schuemann M, Krause E, Suske G, Bauer UM: PRMT1-mediated arginine methylation of PIAS1 regulated STAT1 signaling. Genes Dev. 2009, 23: 118-132. 10.1101/gad.489409.PubMedPubMed CentralView ArticleGoogle Scholar
- Green DM, Marfatia KA, Crafton EB, Zhang X, Cheng X, Corbett AH: Nab2p is required for poly(A) RNA export in Saccharomyces cerevisiae and is regulated by arginine methylation via Hmt1p. J Biol Chem. 2002, 277: 7752-7760. 10.1074/jbc.M110053200.PubMedView ArticleGoogle Scholar
- Lukong KE, Richard S: Arginine methylation signals mRNA export. Nat Struct Mol Biol. 2004, 11: 914-915. 10.1038/nsmb1004-914.PubMedView ArticleGoogle Scholar
- Godin KS, Varani G: How arginine-rich domains coordinate mRNA maturation events. RNA Biol. 2007, 4: 69-75. 10.4161/rna.4.2.4869.PubMedView ArticleGoogle Scholar
- Polevoda B, Sherman F: Methylation of proteins involved in translation. Mol Micro. 2007, 65: 590-606. 10.1111/j.1365-2958.2007.05831.x.View ArticleGoogle Scholar
- Yu MC, Bachand F, McBride AE, Komili S, Casolari JM, Silver PA: Arginine methyltransferase affects interactions and recruitment of mRNA processing and export factors. Genes Dev. 2004, 18: 2024-2035. 10.1101/gad.1223204.PubMedPubMed CentralView ArticleGoogle Scholar
- Xie B, Invernizzi CF, Richard S, Wainberg MA: Arginine methylation of the human immunodeficiency virus type 1 Tat protein by PRMT6 negatively affects Tat interactions with both cyclin T1 and the Tat transactivation region. J Virol. 2007, 81: 4226-4234. 10.1128/JVI.01888-06.PubMedPubMed CentralView ArticleGoogle Scholar
- De Leeuw F, Zhang T, Wauquier C, Huez G, Kruys V, Gueydan C: The cold-inducible RNA-binding protein migrates from the nucleus to cytoplasmic stress granules by a methylation-dependent mechanism and acts as a translational repressor. Exp Cell Res. 2007, 313: 4130-4144. 10.1016/j.yexcr.2007.09.017.PubMedView ArticleGoogle Scholar
- Perreault A, Lemieux C, Bachand F: Regulation of the nuclear poly(A)-binding protein by arginine methylation in fission yeast. J Biol Chem. 2007, 282: 7552-7562.PubMedView ArticleGoogle Scholar
- Smith WA, Schurter BT, Wong-Staal F, David M: Arginine methylation of RNA helicase A determines its subcellular localization. J Biol Chem. 2004, 279: 22795-22798. 10.1074/jbc.C300512200.PubMedView ArticleGoogle Scholar
- Lee DY, Teyssier C, Strahl BD, Stallcup MR: Role of protein methylation in regulation of transcription. Endocr Rev. 2005, 26: 147-170. 10.1385/ENDO:26:2:147.PubMedView ArticleGoogle Scholar
- Côté J, Boisvert FM, Boulanger MC, Bedford MT, Richard S: Sam68 RNA Binding Protein Is an In Vivo Substrate for Protein Arginine N-Methyltransferase 1. Mol Biol Cell. 2003, 14: 274-287. 10.1091/mbc.E02-08-0484.PubMedPubMed CentralView ArticleGoogle Scholar
- Goulah CC, Read LK: Differential effects of arginine methylation on RBP16 mRNA binding, guide RNA (gRNA) binding, and gRNA-containing ribonucleoprotein complex (gRNP) formation. J Biol Chem. 2007, 282: 7181-7190.PubMedView ArticleGoogle Scholar
- McBride AE, Cook JT, Stemmler EA, Rutledge KL, McGrath KA, Rubens JA: Arginine methylation of yeast mRNA-binding protein Npl3 directly affects its function, nuclear export, and intranuclear protein interactions. J Biol Chem. 2005, 280: 30888-30898. 10.1074/jbc.M505831200.PubMedView ArticleGoogle Scholar
- Stetler A, Winograd C, Sayegh J, Cheever A, Patton E, Zhang X, Clarke S, Ceman S: Identification and characterization of the methyl arginines in the fragile X mental retardation protein Fmrp. Hum Mol Genet. 2005, 15: 87-96. 10.1093/hmg/ddi429.PubMedView ArticleGoogle Scholar
- Bedford MT, Richard S: Arginine methylation: An emerging regulator of protein function. Mol Cell. 2005, 18: 263-272. 10.1016/j.molcel.2005.04.003.PubMedView ArticleGoogle Scholar
- McBride AE, Silver PA: State of the Arg: Protein methylation at arginine comes of age. Cell. 2001, 106: 5-8. 10.1016/S0092-8674(01)00423-8.PubMedView ArticleGoogle Scholar
- Pahlich S, Zakaryan RP, Gehring H: Protein arginine methylation: Cellular functions and methods of analysis. Biochim Biophys Acta. 2006, 1764: 1890-1903. 10.1016/j.bbapap.2006.08.008.PubMedView ArticleGoogle Scholar
- Wooderchak WL, Zang T, Zhou ZS, Acuña M, Tahara SM, Hevel JM: Substrate profiling of PRMT1 reveals amino acid sequences that extend beyond the “RGG” paradigm. Biochemistry. 2008, 47: 9456-9466. 10.1021/bi800984s.PubMedView ArticleGoogle Scholar
- Wolf SS: The protein arginine methyltransferase family: an update about function, new perspectives and the physiological role in humans. Cell Mol Life Sci. 2009, 66: 2109-2121. 10.1007/s00018-009-0010-x.PubMedView ArticleGoogle Scholar
- Fisk JC, Read LK: Protein arginine methylation in parasitic protozoa. Eukaryot Cell. 2011, 10: 1013-1022. 10.1128/EC.05103-11.PubMedPubMed CentralView ArticleGoogle Scholar
- Pelletier M, Pasternack DA, Read LK: In vitro and in vivo analysis of the major type I protein arginine methyltransferase from Trypanosoma brucei. Mol Biochem Parasitol. 2005, 144: 206-217. 10.1016/j.molbiopara.2005.08.015.PubMedView ArticleGoogle Scholar
- Pasternack DA, Sayegh J, Clarke S, Read LK: Evolutionarily divergent type II protein arginine methyltransferase in Trypanosoma brucei. Eukaryot Cell. 2007, 6: 1665-1681. 10.1128/EC.00133-07.PubMedPubMed CentralView ArticleGoogle Scholar
- Fisk JC, Sayegh J, Zurita-Lopez C, Menon S, Presnyak V, Clarke SG, Read LK: A type III protein arginine methyltransferase from the protozoan parasite Trypanosoma brucei. J Biol Chem. 2009, 284: 11590-11600.PubMedPubMed CentralView ArticleGoogle Scholar
- Fisk JC, Zurita-Lopez C, Sayegh J, Tomasello DL, Clarke SG, Read LK: TbPRMT6 is a type I protein arginine methyltransferase that contributes to cytokinesis in Trypanosoma brucei. Eukaryot Cell. 2010, 9: 866-877. 10.1128/EC.00018-10.PubMedPubMed CentralView ArticleGoogle Scholar
- Goulah CC, Pelletier M, Read LK: Arginine methylation regulates mitochondrial gene expression in Trypanosoma brucei through multiple effector proteins. RNA. 2006, 12: 1545-1555. 10.1261/rna.90106.PubMedPubMed CentralView ArticleGoogle Scholar
- Berriman M, Ghedin E, Hertz-Fowler C, Blandin G, Renauld H, Bartholomeu DC, Lennard NJ, Caler E, Hamlin NE, Haas B, Böhme U, Hannick L, Aslett MA, Shallom J, Marcello L, Hou L, Wickstead B, Alsmark UC, Arrowsmith C, Atkin RJ, Barron AJ, Bringaud F, Brooks K, Carrington M, Cherevach I, Chillingworth TJ, Churcher C, Clark LN, Corton CH, Cronin A: The genome of African trypanosome Trypanosoma brucei. Science. 2005, 309: 416-422. 10.1126/science.1112642.PubMedView ArticleGoogle Scholar
- Passos DO, Bressan GC, Nery FC, Kobarg J: Ki-1/57 interacts with PRMT1 and is a substrate for arginine methylation. FEBS J. 2006, 273: 3946-3961. 10.1111/j.1742-4658.2006.05399.x.PubMedView ArticleGoogle Scholar
- Reue K, Zhang P: The lipin protein family: dual roles in lipid biosynthesis and gene expression. FEBS Lett. 2008, 582: 90-96. 10.1016/j.febslet.2007.11.014.PubMedPubMed CentralView ArticleGoogle Scholar
- Harris TE, Finck BN: Dual function lipin proteins and glycerolipid metabolism. Trends Endocrinol Metab. 2011, 22: 226-233. 10.1016/j.tem.2011.02.006.PubMedPubMed CentralView ArticleGoogle Scholar
- Inoue K, Mizuno T, Wada K, Hagiwara M: Novel RING Finger proteins, Air1p and Air2p, interact with Hmt1p and inhibit the arginine methylation of Npl3p. J Biol Chem. 2000, 275: 32793-32799.PubMedView ArticleGoogle Scholar
- Tang J, Kao PN, Herschman HR: Protein-arginine methyltransferase I, the predominant protein-arginine methyltransferase in cells, interacts with and is regulated by interleukin enhancer-binding factor 3. J Biol Chem. 2000, 275: 19866-19876. 10.1074/jbc.M000023200.PubMedView ArticleGoogle Scholar
- Hoek M, Zanders T, Cross GAM: Trypanosoma brucei expression-site-associated-gene-8 protein interacts with a Pumilio family protein. Mol Biochem Parasitol. 2002, 120: 269-283. 10.1016/S0166-6851(02)00009-9.PubMedView ArticleGoogle Scholar
- Péterfy M, Xu P, Reue K, Phan: Lipodystrophy in the fld mouse results from mutation of a new gene encoding a nuclear protein, lipin. Nat Genet. 2001, 27: 121-124. 10.1038/83685.PubMedView ArticleGoogle Scholar
- Langner CA, Birkenmeier EH, Roth KA, Bronson RT, Gordon JI: Characterization of the peripheral neuropathy in neonatal and adult mice that are homozygous for the fatty liver dystrophy (fld) mutation. J Biol Chem. 1991, 266: 11955-11964.PubMedGoogle Scholar
- Reue K, Xu P, Wang XP, Slavin BG: Adipose tissue deficiency, glucose intolerance, and increased atherosclerosis result from mutation in the mouse fatty liver dystrophy (fld) gene. J Lipid Res. 2000, 41: 1067-1076.PubMedGoogle Scholar
- Donkor J, Sariahmetoglu M, Dewald J, Brindley DN, Reue K: Three mammalian lipins act as phosphatidate phosphatases with distinct tissue expression patterns. J Biol Chem. 2007, 282: 3450-3457.PubMedView ArticleGoogle Scholar
- Han GS, Wu WI, Carman GM: The Saccharomyces cerevisiae Lipin homolog is a Mg2 + -dependent phosphatidate phosphatase enzyme. J Biol Chem. 2006, 281: 9210-9218.PubMedPubMed CentralView ArticleGoogle Scholar
- Rupali U, Liu Y, Provaznik J, Schmitt S, Lehmann M: Lipin Is a Central Regulator of Adipose Tissue Development and Function in Drosophila melanogaster. Mol Cell Biol. 2011, 31: 1646-1656. 10.1128/MCB.01335-10.View ArticleGoogle Scholar
- Strausberg RL, Feingold EA, Grouse LH, Derge JG, Klausner RD, Collins FS, Wagner L, Shenmen CM, Schuler GD, Altschul SF, Zeeberg B, Buetow KH, Schaefer CF, Bhat NK, Hopkins RF, Jordan H, Moore T, Max SI, Wang J, Hsieh F, Diatchenko L, Marusina K, Farmer AA, Rubin GM, Hong L, Stapleton M, Soares MB, Bonaldo MF, Casavant TL, Scheetz TE: Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proc Natl Acad Sci USA. 2002, 99: 16899-16903. 10.1073/pnas.242603899.PubMedView ArticleGoogle Scholar
- El-Sayed NM, Myler PJ, Bartholomeu DC, Nilsson D, Aggarwal G, Tran AN, Ghedin E, Wourthey EA, Delcher AL, Blandin G, Westenberger SJ, Caler E, Cerqueira GC, Branche C, Haas B, Anupama A, Arner E, Aslund L, Attipoe P, Bontempi E, Bringaud F, Burton P, Cadag E, Campbell DA, Carrington M, Crabtree J, Darban H, da Silveira JF, de Jong P, Edwards K: The genome sequence of Trypanosoma cruzi, etiologic agent of Chagas disease. Science. 2005, 309: 409-415. 10.1126/science.1112631.PubMedView ArticleGoogle Scholar
- Siniossoglou S: Lipins, lipids and nuclear envelope structure. Traffic. 2009, 10: 1181-1187. 10.1111/j.1600-0854.2009.00923.x.PubMedView ArticleGoogle Scholar
- Finck BN, Gropler MC, Chen Z, Leone TC, Croce MA, Harris TE, Lawrence JC, Kelly DP: Lipin 1 is an inducible amplifier of the hepatic PGC-1alpha/PPARalpha regulatory pathway. Cell Metab. 2006, 4: 199-210. 10.1016/j.cmet.2006.08.005.PubMedView ArticleGoogle Scholar
- Harris TE, Huffman TA, Chi A, Shabanowitz J, Hunt DF, Kumar A, Lawrence JC: Insulin controls subcellular localization and multisite phosphorylation of the phosphatidic acid phosphatase, lipin 1. J Biol Chem. 2007, 282: 277-286.PubMedView ArticleGoogle Scholar
- Péterfy M, Phan J, Reue K: Alternatively spliced lipin isoforms exhibit distinct expression pattern, subcellular localization, and role in adipogenesis. J Biol Chem. 2005, 280: 32883-32889. 10.1074/jbc.M503885200.PubMedView ArticleGoogle Scholar
- Péterfy M, Harris TE, Fujita N, Reue K: Insulin-stimulated interaction with 14-3-3 promotes cytoplasmic localization of lipin-1 in adipocytes. J Biol Chem. 2010, 285: 3857-3864. 10.1074/jbc.M109.072488.PubMedPubMed CentralView ArticleGoogle Scholar
- Duan P, Xu Y, Birkaya B, Myers J, Pelletier M, Read LK, Guarnaccia C, Pongor S, Denman RB, Aletta JM: Generation of polyclonal antiserum for the detection of methylarginine proteins. J Immunol Methods. 2007, 320: 132-142. 10.1016/j.jim.2007.01.006.PubMedPubMed CentralView ArticleGoogle Scholar
- Koonin EV, Tatusov RL: Computer analysis of bacterial haloacid dehalogenases defines a large superfamily of hydrolases with diverse specificity. Application of an iterative approach to database search. J Mol Biol. 1994, 244: 125-132. 10.1006/jmbi.1994.1711.PubMedView ArticleGoogle Scholar
- Hisano T, Hata Y, Fujii T, Liu JQ, Kurihara T, Esaki N, Soda K: Crystal structure of L-2 haloacid dehalogenase from Pseudomonas sp. YL. J Biol Chem. 1996, 34: 20322-20330.View ArticleGoogle Scholar
- Huffman TA, Mothe-Satney I, Lawrence JC: Insulin-stimulated phosphorylation of lipin mediated by the mammalian target of rapamycin. Proc Natl Acad Sci USA. 2002, 99: 1047-1052. 10.1073/pnas.022634399.PubMedPubMed CentralView ArticleGoogle Scholar
- O’Hara L, Han G-S, Peak-Chew S, Grimsey N, Carman GM, Siniossoglou S: Control of phospholipid synthesis by phosphorylation of the yeast lipin Pah1p/Smp2p Mg2+-dependent phosphatidate phosphatase. J Biol Chem. 2006, 281: 34537-34548. 10.1074/jbc.M606654200.PubMedPubMed CentralView ArticleGoogle Scholar
- Santos-Rosa H, Leung J, Grimsey N, Peak-Chew S, Siniossoglou S: The yeast lipin Smp2 couples phospholipid biosynthesis to nuclear membrane growth. EMBO J. 2005, 24: 1931-1941. 10.1038/sj.emboj.7600672.PubMedPubMed CentralView ArticleGoogle Scholar
- Nett IRE, Martin DMA, Miranda-Saavedra D, Lamont D, Barber JD, Mehlert A, Ferguson MAJ: The phosphoproteome of bloodstream form Trypanonosoma brucei, causative agent of African Sleeping Sickness. Mol Cell Proteomics. 2009, 8: 1527-1538. 10.1074/mcp.M800556-MCP200.PubMedPubMed CentralView ArticleGoogle Scholar
- Cheng D, Côté J, Shaaban S, Bedford MT: The arginine methyltransferase CARM1 regulates the coupling of transcription and mRNA processing. Mol Cell. 2007, 25: 71-83. 10.1016/j.molcel.2006.11.019.PubMedView ArticleGoogle Scholar
- Côté J, Richard S: Tudor domains bind symmetrical dimethylated arginines. J Biol Chem. 2005, 280: 28476-28483. 10.1074/jbc.M414328200.PubMedView ArticleGoogle Scholar
- Kim S, Merrill BM, Rajpurohit R, Kumar A, Stone KL, Papov VV, Schneiders JM, Szer W, Wilson SH, Paik WK, Williams KR: Identification of N(G)-methylarginine residues in human heterogeneous RNP protein A1: Phe/Gly-Gly-Gly-Arg-Gly-Gly-Gly/Phe is a preferred recognition motif. Biochemistry. 1997, 36: 5185-5192. 10.1021/bi9625509.PubMedView ArticleGoogle Scholar
- Liu Q, Dreyfuss G: In vivo and in vitro arginine methylation of RNA-binding proteins. Mol Cell Biol. 1995, 15: 2800-2808.PubMedPubMed CentralView ArticleGoogle Scholar
- Najbauer J, Johnson BA, Young AL, Aswad DW: Peptides with sequences similar to glycine, arginine-rich motifs in proteins interacting with RNA are efficiently recognized by methyltransferase(s) modifying arginine in numerous proteins. J Biol Chem. 1993, 268: 10501-10509.PubMedGoogle Scholar
- Vance JE, Vance DE: Phospholipid biosynthesis in mammalian cells. Biochem Cell Biol. 2004, 82: 113-128. 10.1139/o03-073.PubMedView ArticleGoogle Scholar
- Kennedy EP, Weiss SB: The function of cytidine coenzymes in the biosynthesis of phospholipids. J Biol Chem. 1956, 222: 193-214.PubMedGoogle Scholar
- Vance JE, Steenbergen R: Metabolism and functions of phosphatidylserine. Prog Lipid Res. 2005, 44: 207-234. 10.1016/j.plipres.2005.05.001.PubMedView ArticleGoogle Scholar
- Smith TK, Bütikofer P: Lipid metabolism in Trypanosoma brucei. Mol Biochem Parasitol. 2010, 172: 66-79. 10.1016/j.molbiopara.2010.04.001.PubMedPubMed CentralView ArticleGoogle Scholar
- Martin KL, Smith TK: Phosphatidylinositol synthesis is essential in bloodstream form Trypanosoma brucei. Biochem J. 2006, 396: 287-295. 10.1042/BJ20051825.PubMedPubMed CentralView ArticleGoogle Scholar
- Signorell A, Rauch M, Jelk J, Ferguson MAJ, Bütikofer P: Phosphatidylethanolamine in Trypanosoma brucei is organized in two separate pools and is synthesized exclusively by the Kennedy Pathway. J Biol Chem. 2008, 283: 23636-23644. 10.1074/jbc.M803600200.PubMedPubMed CentralView ArticleGoogle Scholar
- Ferguson MAJ: The structure, biosynthesis and functions of glycosylphosphatidylinositol anchors, and the contributions of trypanosome research. J Cell Science. 1999, 112: 2799-2809.PubMedGoogle Scholar
- Martin KL, Smith TK: The glycosylphosphatidylinositol (GPI) biosynthetic pathway of bloodstream form Trypanosoma brucei is dependent on the de novo synthesis of inositol. Mol Microbiol. 2006, 61: 89-105. 10.1111/j.1365-2958.2006.05216.x.PubMedPubMed CentralView ArticleGoogle Scholar
- Menon AK, Eppinger M, Mayor S, Schwarz RT: Phosphatidylethanolamine is the donor of the terminal phosphoethanolamine group in trypanosome glycosylphosphatidylinositols. EMBO J. 1993, 12: 1907-1914.PubMedPubMed CentralGoogle Scholar
- Gibellini F, Hunter WN, Smith TK: The ethanolamine branch of the Kennedy pathway is essential in the bloodstream form of Trypanosoma brucei. Mol Microbiol. 2009, 73: 826-843. 10.1111/j.1365-2958.2009.06764.x.PubMedPubMed CentralView ArticleGoogle Scholar
- Brun R, Schonenberg M: Cultivation and in vitro cloning of procyclic culture forms of Trypanosoma brucei in a semi-defined medium. Acta Trop. 1979, 36: 289-292.PubMedGoogle Scholar
- Gietz D, St-Jean A, Woods RA, Schiestl RH: Improved method for high efficiency transformation of intact yeast cells. Nucleic Acids Res. 1992, 20: 1425-10.1093/nar/20.6.1425.PubMedPubMed CentralView ArticleGoogle Scholar
- Hayman ML, Miller MM, Chandler DM, Goulah CC, Read LK: The trypanosome homolog of human p32 interacts with RBP16 and stimulates its gRNA binding activity. Nucleic Acids Res. 2001, 29: 5216-5225. 10.1093/nar/29.24.5216.PubMedPubMed CentralView ArticleGoogle Scholar
- Zeiner GM, Sturm NR, Campbell DA: Exportin 1 mediates nuclear export of the kinetoplastid spliced leader RNA. Eukaryot Cell. 2003, 2: 222-230. 10.1128/EC.2.2.222-230.2003.PubMedPubMed CentralView ArticleGoogle Scholar
- Chapman AB, Agabian N: Trypanosoma brucei RNA polymerase II is phosphorylated in the absence of carboxyl-terminal domain heptapeptide repeats. J Biol Chem. 1994, 269 (7): 4754-4760.PubMedGoogle Scholar