sigE facilitates the adaptation of Bordetella bronchiseptica to stress conditions and lethal infection in immunocompromised mice
© Barchinger et al.; licensee BioMed Central Ltd. 2012
Received: 7 December 2011
Accepted: 25 June 2012
Published: 16 August 2012
The cell envelope of a bacterial pathogen can be damaged by harsh conditions in the environment outside a host and by immune factors during infection. Cell envelope stress responses preserve the integrity of this essential compartment and are often required for virulence. Bordetella species are important respiratory pathogens that possess a large number of putative transcription factors. However, no cell envelope stress responses have been described in these species. Among the putative Bordetella transcription factors are a number of genes belonging to the extracytoplasmic function (ECF) group of alternative sigma factors, some of which are known to mediate cell envelope stress responses in other bacteria. Here we investigate the role of one such gene, sigE, in stress survival and pathogenesis of Bordetella bronchiseptica.
We demonstrate that sigE encodes a functional sigma factor that mediates a cell envelope stress response. Mutants of B. bronchiseptica strain RB50 lacking sigE are more sensitive to high temperature, ethanol, and perturbation of the envelope by SDS-EDTA and certain β-lactam antibiotics. Using a series of immunocompromised mice deficient in different components of the innate and adaptive immune responses, we show that SigE plays an important role in evading the innate immune response during lethal infections of mice lacking B cells and T cells. SigE is not required, however, for colonization of the respiratory tract of immunocompetent mice. The sigE mutant is more efficiently phagocytosed and killed by peripheral blood polymorphonuclear leukocytes (PMNs) than RB50, and exhibits decreased cytotoxicity toward macrophages. These altered interactions with phagocytes could contribute to the defects observed during lethal infection.
Much of the work on transcriptional regulation during infection in B. bronchiseptica has focused on the BvgAS two-component system. This study reveals that the SigE regulon also mediates a discrete subset of functions associated with virulence. SigE is the first cell envelope stress-sensing system to be described in the bordetellae. In addition to its role during lethal infection of mice deficient in adaptive immunity, our results indicate that SigE is likely to be important for survival in the face of stresses encountered in the environment between hosts.
KeywordsB. bronchiseptica Extracytoplasmic function sigma factor Cell envelope stress Pathogenesis
The cell envelope of bacterial pathogens is critical for survival both in a host during infection and in the environment outside of the host. As the interface between the bacterium and the outside milieu, the cell envelope acts as a barrier protecting the cell against extracellular hazards. Cell envelope structures are also intimately involved in the formation of contacts with host tissues during infection. To safeguard this important compartment, gram-negative bacteria possess an array of stress responses that sense conditions in the cell envelope and alter gene expression to ensure its integrity [1, 2]. In many bacterial pathogens, cell envelope stress responses play a multifaceted role. They provide protection against damage caused by components of the immune system, such as complement and antimicrobial peptides that target the cell envelope [3–5]. They regulate the expression of chaperones required for proper assembly of cell envelope-associated structures, including outer membrane porins, pili, and fimbrae [3, 6, 7]. In addition, cell envelope stress responses can sense the environment around the bacterium and regulate the expression of virulence factors in response to specific cues, ensuring that these factors are expressed at the proper time and location in the host [2, 8]. Despite their importance, no cell envelope stress responses have yet been identified or implicated in pathogenesis in Bordetella species.
Bordetella bronchiseptica is a respiratory pathogen that is closely related to Bordetella pertussis and Bordetella parapertussis, the causative agents of whooping cough in humans [9, 10]. B. bronchiseptica causes a range of diseases in various mammals that can be chronic, difficult to completely eradicate, and of variable virulence [11–13]. It is the etiological agent of atrophic rhinitis in swine, kennel cough in dogs, and snuffles in rabbits [12, 13]. Documented human infections, generally traced to an animal source, have been observed in immunocompromised individuals, and can be serious, systemic infections [11, 14].
The B. bronchiseptica, B. pertussis and B. parapertussis genomes encode a large number of putative transcription factors relative to their overall genome size , suggesting that these pathogens have the capacity to extensively regulate gene expression in response to environmental and physiological changes. Despite this finding, only a few Bordetella transcription factors have been studied in any detail [16–20]. Among the predicted transcription factors is an ortholog of the cell envelope stress response sigma factor, σE, of E. coli. In bacteria, sigma factors are the subunits of bacterial RNA polymerases required for specific promoter recognition and transcription initiation . Alternative sigma factors, like σE, are activated in response to specific stresses and rapidly reprogram gene expression by replacing the housekeeping sigma factor and directing RNA polymerase to the genes in their regulons [21, 22].
σE belongs to the RpoE-like group of extracytoplasmic function (ECF) sigma factors that have been increasingly implicated as key factors contributing to both bacterial stress responses and virulence [23, 24]. These sigma factors are widely distributed across bacterial phyla. Where studied, they direct a diverse set of stress responses primarily targeted to the cell envelope [2, 8, 24, 25]. In E. coli and Salmonella enterica serovar Typhimurium, σE controls many genes whose products are required for the proper expression of outer membrane porins and LPS [26, 27]. During infection, σE of S. Typhimurium is required for survival and proliferation in epithelial and macrophage cell lines, and in the presence of antimicrobial peptides [6, 28, 29]. In Pseudomonas aeruginosa, the σE homologue, AlgU, controls the expression of the exopolysaccharide alginate and conversion to mucoidy. AlgU is constitutively activated in many clinical isolates from cystic fibrosis patients [30, 31]. In addition, σE is required for the viability of some bacterial species, but not others. The gene encoding σE is essential in E. coli and Yersinia enterocolitica, but is dispensable in the closely related species S. Typhimurium [6, 32, 33]. These observations suggest that the functions of σE orthologs have been adapted to combat the challenges each organism faces in its particular environmental niche. By exploring the role of σE in diverse bacterial species, we can learn which aspects of this widespread regulatory pathway are universally conserved and which have diverged over the course of evolution.
Here we show that the B. bronchiseptica σE ortholog, encoded by the gene sigE (BB3752), is an active sigma factor that mediates a cell envelope stress response. This is the first demonstration of an envelope stress-sensing system in Bordetella species. Using a murine infection model, we demonstrate that SigE plays an important role during lethal infection in mice lacking adaptive immunity, but not in respiratory tract colonization. This finding has important implications for human disease, given the observation that B. bronchiseptica can cause serious systemic infections in immunocompromised humans [11, 14]. This study suggests that SigE is a critical factor in this process, in addition to the BvgAS master virulence regulatory system.
sigEencodes an active sigma factor
To provide additional evidence that SigE is a functional sigma factor, N-terminally His-tagged SigE was purified and tested for its ability to initiate transcription in vitro from the E. coli rpoHP3 promoter. Holoenzyme formed with SigE and E. coli core RNA polymerase (ESigE) was able to direct transcription and produced a transcript of equivalent length to that generated by E. coli EσE (Figure 1C). The region immediately upstream of the B. bronchiseptica rpoH homologue, encoded by the fam gene, contains a sequence that is similar to the proposed σE-dependent consensus promoter, suggesting that B. bronchiseptica rpoH is regulated by SigE. Indeed, SigE was able to direct transcription from the putative fam promoter region in vitro (Figure 1C). Taken together, these results demonstrate that SigE is a functional sigma factor and can initiate transcription from promoter sequences similar to those utilized by other members of the RpoE-like sigma factor family.
sigE contributes to the B. bronchisepticastress response
To investigate whether SigE mediates a cell envelope stress response in B. bronchiseptica, we used disk diffusion assays to compare the sensitivity of RB50 and RB50ΔsigE to several chemicals that compromise cell envelope integrity and a series of antibiotics that block different steps in peptidoglycan synthesis. The sigE mutant was more sensitive than the wild-type strain to the detergent SDS in combination with EDTA (Figure 2B). The sigE mutant was also more sensitive than wild-type RB50 to the antibiotics mecillinam and ampicillin (Figure 2B), whereas sensitivity to meropenem, aztreonam, and imipenem was not affected (data not shown). Unlike σE orthologs in other bacteria, SigE was not required for resistance to the cationic antimicrobial peptide polymyxin B, which targets bacterial membranes, or to osmotic stress (Figure 2B and data not shown) [6, 36, 38, 39]. RB50ΔsigE and RB50 were also equally sensitive to antibiotics that inhibit cytoplasmic processes such as translation (chloramphenicol, erythromycin, kanamycin, tetracycline), transcription (rifampicin), and cytoplasmic enzymes such as DNA gyrase (nalidixic acid), and dihydrofolate reductase (trimethoprim) (data not shown). This lack of sensitivity to multiple antibiotics suggests that the sigE mutation does not lead to an overall increase in the permeability of the outer membrane, which would allow more of the antibiotic to enter the cell. These results show that SigE is important for survival in response to specific types of damage to the cell envelope, such as disruption of cellular membranes caused by SDS/EDTA and interference with synthesis of the peptidoglycan layer caused by ampicillin and mecillinam.
We next asked if sigE is important for survival following a shift to high temperature, which perturbs both the cell envelope and cytoplasm. RB50 and RB50ΔsigE were grown at 37°C to an OD600 of 0.4, then shifted to 50°C, a lethal temperature for B. bronchiseptica. Cell viability, assessed by CFU/ml, was measured after the shift to 50°C. Survival of the RB50ΔsigE strain was lower than that of RB50 (Figure 2C). In attempting to complement this phenotype, we found that plasmid-encoded sigE did not restore survival during heat shock (data not shown), although it did complement other phenotypes, as described below. Similar variability in complementation of a σE mutant by a plasmid-encoded rpoE gene has been seen in other bacteria [29, 36, 40, 41]. Work from Burkholderia cenocepacia showed that expressing σE from a plasmid actually increased sensitivity to heat stress . In S. Typhimurium, an rpoE mutant was sensitive to paraquat and did not survive in stationary phase under anaerobic conditions. Expression of rpoE from a plasmid partially complemented the former phenotype, but not the latter . Because the anti-sigma factor that regulates σE activity was not included in any of these instances, it is likely that proper regulation of SigE activity is required for optimal response to particular stresses, not merely excess SigE activity, complicating complementation experiments.
Another aspect of the classical heat shock response is thermotolerance. When bacteria are exposed to an elevated but nonlethal temperature, heat shock responses are induced, resulting in increased production of chaperones and proteases that refold or degrade unfolded proteins . Consequently, the cells are preloaded with protective factors and exhibit increased survival following a subsequent shift to a lethal temperature . To investigate the role of SigE in this phenomenon, RB50 and RB50ΔsigE were grown to an OD600 of 0.1 at 37°C, shifted to 40°C for 90 min, then shifted to 50°C. RB50 cultures incubated at 40°C before 50°C survived better at all time points than those directly shifted from 37°C to 50°C. For example, 54% of the RB50 cells pre-adapted at 40°C survived two hours after the shift to 50°C (Figure 2C) compared to 0.1% survival for those shifted directly from 37°C to 50°C (Figure 2C). RB50ΔsigE pre-adapted at 40°C also survived better at 50°C than when directly shifted from 37°C to 50°C. However, only 38% of the RB50ΔsigE cells survived after one hour (compared to 76% of the wild-type RB50), and 5% survived after two hours at 50°C (Figure 2C). These results demonstrate that B. bronchiseptica exhibits a classical thermotolerance response and that SigE contributes to this response.
σE homologues have also been found to play a role during oxidative stress in S. Typhimurium and Burkholderia pseudomallei[29, 41]. However, in disk diffusion assays, SigE was not required for survival in the presence of hydrogen peroxide or paraquat, two inducers of oxidative stress (data not shown). Either SigE is not involved in combating oxidative stress in B. bronchiseptica, or other oxidative-stress responsive pathways compensate for SigE when it is absent.
Growth in the murine respiratory tract is not affected by the lack of sigE
B. bronchiseptica RB50 colonizes the respiratory tract of immunocompetent mice, causing an asymptomatic infection that is eventually cleared by the immune system. To determine whether B. bronchiseptica SigE contributes to colonization and persistence in the respiratory tract, groups of C57BL/6 mice were inoculated with RB50 or RB50ΔsigE. Colonization was measured in the nasal cavity, trachea, and lung on days 0, 3, 7, 14, 28 and 63 post-inoculation. Both wild-type and sigE-deficient RB50 colonized the nasal cavity at comparable levels, peaking on day 3 post-inoculation, and stabilizing at about 104-5 CFU by 2 weeks post-inoculation (Figure 3). Both strains also showed similar colonization kinetics in the lower respiratory tract of C57BL/6 mice, peaking in numbers on days 3 and 7 post-inoculation in the trachea and lungs, respectively, and declining thereafter, with complete clearance in both organs by day 63 post-inoculation (Figure 3). These data indicate that B. bronchiseptica SigE is not required for colonization or persistence in the murine respiratory tract.
SigE contributes to lethal B. bronchisepticainfection in mice lacking B cells and T cells, but not in mice lacking TLR4 or TNF-α
B. bronchiseptica has been observed to cause a range of disease including bronchitis, lethal pneumonia, and even systemic infection [11, 12]. Mice with defined immune deficiencies are particularly susceptible to different forms of disease [44–46], facilitating assessment of the roles of specific bacterial factors/functions in interactions with different aspects of the host immune response.
Mice lacking key components of innate immunity, either TLR4 or TNF-α, were challenged with RB50 or RB50ΔsigE and signs of severe disease were monitored. Consistent with published studies, TLR4def and TNF-α−/− mice inoculated with 105 CFU of RB50 quickly developed signs of lethal bordetellosis such as ruffled fur, hunched posture, decreased activity, and difficulty breathing, and succumbed 2 to 5 days post-inoculation [46, 47]. Mice challenged with RB50ΔsigE also showed similar signs of disease and time to death (data not shown). In a separate experiment, TLR4def mice and TNF-α−/− mice infected with RB50 or RB50ΔsigE that were still alive by day 3 post-inoculation were dissected for bacterial enumeration in the respiratory as well as systemic organs. Both wild-type and sigE-deficient RB50 colonized the lungs of TLR4def mice at 107-8 CFU, which was almost 1000-fold higher than in the lungs of TLR4suf mice. Moreover, both strains colonized the systemic organs in TLR4def, but not TLR4suf mice (data not shown). Both strains also grew to higher numbers in the lungs of TNF-α−/− mice than in the lungs of C57BL/6 mice and were recovered from systemic organs only in TNF-α−/− mice (data not shown). These data indicate that SigE is not required for B. bronchiseptica to cause lethal infection and colonize systemic organs in mice lacking TLR4 or TNF-α.
The failure of RB50ΔsigE to colonize distal organs of Rag1−/− mice suggests that this mutant may be defective in getting into or survival in the bloodstream and/or systemic organs. The bloodstream includes many important bactericidal factors of the host immune system, including complement and phagocytes. We first examined whether B. bronchiseptica lacking sigE is more susceptible to complement-mediated killing. 500 CFU of RB50, RB50ΔsigE, or RB50Δwbm, a strain lacking O-antigen, which is known to be susceptible to complement , were incubated at 37°C for one hour in PBS with 20% complement-active or complement-inactive serum from naïve mice. The survival of RB50ΔsigE and RB50 was not affected by the presence of either serum (data not shown). In contrast, the RB50Δwbm strain was almost completely killed by complement-active, but not complement-inactive serum (0.7% survival in the presence of complement-active serum compared to 100% survival in the presence of complement-inactive serum). The observation that RB50ΔsigE survived in the presence of serum without B. bronchiseptica-specific antibodies indicates that the defect in causing systemic infection in mice lacking B and T cells is not due to failure to survive the antimicrobial components in serum, including complement.
SigE contributes to cytotoxicity to macrophages
RB50ΔsigEis more efficiently phagocytosed and killed by PMNs
The BvgAS system of the bordetellae plays a central role in regulating gene expression during pathogenesis [50–52]. However, other regulators may be required during the infectious disease cycle, as Bordetella genomes have a large number of putative sensory systems [10, 16–20]. In this study, we focused on cell envelope sensing systems and investigated the alternative sigma factor, SigE. We found that SigE of B. bronchiseptica does indeed mediate a protective cell envelope stress response and that strains lacking SigE do not establish lethal infections in mice lacking adaptive immunity. These data suggest that the role of SigE is to combat stresses to the envelope imposed by the immune system within a host and by harsh conditions in the environment outside a host. This work is the first demonstration of a cell envelope sensing system in the bordetellae. The σE system has been explored in the most depth in enteric pathogens belonging to the Gammaproteobacteria [23, 25, 53]. The bordetellae, members of the Betaproteobacteria, encounter distinctly different environments in the respiratory tract and therefore provide an excellent model to study how the SigE system has been adapted throughout evolution to serve the needs of diverse bacterial pathogens.
The entire sigE locus (BB3752-BB3750) is identical at the amino acid sequence level among the classical bordetellae, suggesting a conserved role in the human pathogens B. pertussis and B. parapertussis. However, the lifestyles and, therefore, conditions encountered differ amongst these three species. B. bronchiseptica can live outside the host and primarily infects mammals, although it can infect immunocompromised humans [11, 14]. In contrast, B. pertussis and B. parapertussis primarily infect humans and are directly transmitted between hosts [54, 55]. As we learn more about the role of SigE in the bordetellae, it will be of interest to determine whether stresses that induce the SigE system and the SigE regulon members are as highly conserved as the sigE locus itself among the bordetellae.
Our results define roles for SigE in B. bronchiseptica that are only partially overlapping with those for σE in other pathogens. SigE was important for survival of B. bronchiseptica in the face of both global stresses to the cell envelope caused by heat shock, exposure to ethanol and detergent, and specific stresses caused by several beta-lactam antibiotics (Figure 2). Heat shock, ethanol, and detergent are classical stressors used in the laboratory to mimic conditions that lead to unfolded proteins and disrupted lipids during infection and in the environment. In contrast to the B. cenocepacia and S. Typhimurium proteins, B. bronchiseptica SigE was not required for survival during osmotic stress [6, 36]. SigE was also not required for response to oxidative stress or the antimicrobial peptide polymyxin B, unlike the S. Typhimurium σE ortholog [6, 29]. The variations among bacteria in their use of σE systems likely reflect both differences in stresses encountered in environmental reservoirs and in particular host tissues during infection, as well as differences in the arrays of additional cellular stress responses possessed by each species. These other responses can act along with or in place of σE. The presence of other stress responses may be particularly pertinent to B. bronchiseptica. Its genome is predicted to encode six related ECF sigma factors of unknown function in addition to SigE  that may have complimentary and redundant functions with SigE. Future studies defining conditions that activate other ECF sigma factors and their roles in B. bronchiseptica pathogenesis will provide a more comprehensive understanding of how B. bronchiseptica copes with extracytoplasmic stress.
Stress response systems, like the σE system, rapidly induce the expression of specialized sets of genes. These systems are often tightly regulated and expressed only when needed, because inappropriate expression of their regulons can interfere with other important cellular functions [8, 56, 57]. We found that SigE was not required for colonization and persistence of RB50 within the respiratory tract of an immunocompetent host (Figure 3), the primary niche of B. bronchiseptica. This result suggests that the pathogen does not encounter stresses in the respiratory tract that require a response by the SigE system. However, B. bronchiseptica encounters different challenges during infection in Rag1−/− mice lacking B and T cells. In these mice, the infection spreads to the bloodstream, which is under greater immune surveillance and has a different arsenal of antimicrobial factors to attack invaders than the respiratory tract. The defect of RB50ΔsigE in lethal infection of Rag1−/− mice, therefore, reveals a specific function for SigE in response to an unknown stress, possibly related to the innate immune response, that the bacteria encounter during infections that proceed beyond colonization of the respiratory tract.
The inability of RB50ΔsigE to cause lethal infections in Rag1−/− mice (Figure 4) could be due to failure to enter or survive in the bloodstream and/or systemic organs of these mice. Since the mutation does not affect survival during incubation with serum in vitro, it is unlikely that the sigE-deficient strain is more susceptible to complement or other antimicrobial components in serum. The defect in infection of Rag1−/− mice may then be related to altered interactions of the mutant strain with phagocytic cells in the bloodstream. RB50ΔsigE is more susceptible to peripheral blood PMNs than RB50 (Figure 6), and is also less cytotoxic to macrophages than RB50 (Figure 5). Either or both of these defects could explain the failure to recover RB50ΔsigE from systemic organs of mice lacking adaptive immune responses and the decreased virulence in these mice.
Why does the RB50ΔsigE mutant spread systemically and cause lethal infection in TLR4def and TNF-α−/− mice, but not Rag1−/− mice? The lower cytotoxicity of the sigE mutant and its increased sensitivity to phagocytic killing does not affect its virulence in mice lacking innate immune functions. This could be because bacterial numbers within the respiratory tract of TLR4def or TNF-α−/− mice are nearly an order of magnitude higher than in the lungs of Rag1−/− mice. As such, the large number of bacteria in TLR4def or TNF-α−/− mice may overwhelm limiting host antimicrobial defense mechanisms that can contain the lower bacterial numbers in the lungs of Rag1−/− mice. Alternatively, although the cytotoxicity of the sigE mutant is reduced, it may still be sufficient to establish lethal infections in the absence of TLR4 or TNF-α. Thus TLR4- and TNF-α-dependent functions, such as efficient phagocytosis and killing, appear to be sufficient to prevent lethal infection by RB50ΔsigE in Rag1−/− mice. Although the exact role remains to be elucidated, our results clearly indicate that SigE is required for lethal infection of mice lacking B and T cells.
Although the B. bronchiseptica strain RB50 causes asymptomatic infections in immunocompetent mice, other strains of B. bronchiseptica can cause a wide range of disease severity in other hosts [11–13]. In particular subsets of immunocompromised humans, such as those infected with HIV, severe systemic B. bronchiseptica infections have been observed . These facts, along with the high degree of sequence conservation for the sigE locus in B. pertussis and B. parapertussis, highlights the importance of understanding the stressors that activate SigE and how the SigE system responds to them during infection.
In this work, we have demonstrated that the B. bronchiseptica extracytoplasmic function sigma factor, SigE, is important for surviving global stresses that affect the whole cell, such as heat shock and ethanol stress, specific stresses that target the cell envelope, such as beta-lactam antibiotics and SDS-EDTA, and in interactions with the host innate immune system, particularly phagocytes. During infection, SigE is not required for colonization of the respiratory tract of immunocompetent mice. However, it is needed for a specific set of functions associated with virulence, particularly those involved in surviving the innate immune response when the infection progresses in immunocompromised mice. Although SigE systems are widely conserved, the details as to which aspects are shared and which have diverged are complex. As evidence accumulates from studies in different bacteria, it is becoming apparent that these sensory modules are important for stress survival, particularly with respect to the cell envelope. However, the nature of the stresses that SigE systems combat varies. During infection, comparisons are even more difficult, since differences are seen not only amongst SigE systems from one pathogen to another, but also within different niches in the host or during the progression of disease for a single pathogen.
Strains and media
Strains and plasmids
MG1655 ΦλrpoHP3::lacZ ΔlacX74
BL21(DE3) ΔslyD::kan pLysS pPER76
BL21(DE3) ΔslyD::kan pLysS pXQZ001
CAG43113 ΔrpoE::kan ΔnadB::Tn10
Vector, pBR322 ori, ApR
sigE in pTrc99a
isolated rpoHP3 promoter in pRLG770, ApR
rpoE in T7 expression vector pET15b, KanR
sigE in T7 expression vector pET15b, KanR
ΔsigE in TOPO-TA vector
helper plasmid competent for mating, ApR
Bordetella-specific allelic exchange vector, KanR
Stibitz, unpublished work
ΔsigE in pSS3962
Vector pJS72, ΩSpecR cassette replaced with CmR
sigE in pEV
cytoplasmic expression of GFP
Sequence (5´ - 3´)
Source or Reference
Construction of RB50ΔsigEstrain
The sigE gene was deleted from RB50 using a Bordetella-specific allelic exchange procedure to produce strain SEA5516. Primers used in the construction are listed in Table 2. A PCR product containing 637 bp proximal to the 5' end of sigE was amplified from RB50 genomic DNA using primers SigEKO_LeftF and SigEKO_LeftR. A non-overlapping PCR product containing 534 bp proximal to the 3' end of sigE was amplified with primers SigEKO_RightF and SigEKO_RightR. The two fragments were digested with BamHI and ligated. The resulting construct was amplified with primers SigEKO_LeftF and SigEKO_RightR, cloned into the TopoTA vector (Invitrogen), and verified by sequencing to give plasmid pXQ002. In this deletion construct, the 528 bp central region of the sigE gene is deleted leaving 66 bp at the 5' end and 6 bp at the 3' end of the sigE gene. The deletion construct from pXQ002 was then cloned into the EcoRI site of the allelic exchange vector pSS3962 (Stibitz S., unpublished data) to generate pXQ003 and transformed into E. coli strain DH5α. Tri-parental mating with wild-type B. bronchiseptica strain RB50, E. coli strain DH5α harboring the pXQ003 vector (strain XQ003), and DH5α harboring the helper plasmid pSS1827 (strain SS1827) [69, 70] and selection of mutants were performed as previously described . The deletion strain was verified by PCR using primers SigEKO_LeftF and SigEKO_RightR and by Southern blot analysis.
Overnight cultures were diluted into fresh medium and grown to an OD600 of 0.1-0.2 at 30°C. Where indicated, IPTG was added to a final concentration of 1 mM. Samples were collected 2.5 hours later and β-galactosidase activity from the σE-dependent reporter was assayed as previously described [60, 71].
Complementation of E. coli ΔrpoE by B. bronchiseptica sigE
The ability of B. bronchiseptica sigE to suppress the lethality caused by deletion of rpoE in E. coli was determined using a cotransduction assay as described . The ΔrpoE::kan ΔnadB::Tn10 allele from strain SEA4114 was moved via P1 transdution into strain SEA5005, which carries sigE on the plasmid pSEB006. Tet-resistant (tetR) transductants were selected and then screened for kanamycin resistance (kanR). Although the nadB and rpoE alleles are tightly linked (>99%), cotransduction resulting in tetR kanR colonies will only occur if rpoE is no longer essential for viability. In transductions with E. coli expressing sigE (strain SEA5005) as the recipient strain, 31 out of 32 tetR transductants were also kanR. In contrast, none of the 39 tetR transductants were kanR when E. coli carrying the empty cloning vector (strain SEA008) was the recipient strain.
N-terminally His-tagged B. bronchiseptica SigE and E. coli σE were purified from strain XQZ001 and SEA5036, respectively, as previously described for E. coli σE. Briefly, cells were grown at 25°C to an OD600 of 0.5, at which point IPTG was added to induce protein production. Following 1.5-3 hours of induction, cells were harvested by centrifugation and resuspended in lysis buffer (20 mM Tris–HCl pH 8.0, 500 mM NaCl, 20 mM imidazole, 2.5 mM β-mercaptoethanol, 1 mM PMSF). Resuspended cells were then lysed by sonication, and the lysate cleared by centrifugation. The supernatant containing soluble His-SigE was loaded onto a Ni-NTA column (Qiagen). Bound proteins were eluted with a stepwise gradient of 20, 60, 100, and 200 mM imidazole in column buffer (20 mM Tris–HCl pH 8.0, 500 mM NaCl, 2.5 mM β–mercaptoethanol). Fractions containing SigE were pooled and dialyzed into 20 mM Tris–HCl pH 8.0, 50 mM NaCl, and 2.5 mM β-mercaptoethanol.
In vitro transcription
100 nM E. coli core RNA polymerase (Epicentre) was incubated with 400 nM His-SigE or His-σE in transcription buffer (40 mM Tris–HCl pH 8.0, 10 mM MgCl2, 50 mM NaCl, 1 mM DTT, 0.1 μ/ml BSA) for 10 min at 30°C to form holoenzyme. Multi-round transcription reactions were initiated by addition of holoenzyme at a final concentration of 40 nM sigma factor and 10 nM core RNA polymerase, to prewarmed (30°C) transcription mix containing 5.0 nM supercoiled plasmid template pSEB015  or 5.0 nM linear Pfam template, 5% glycerol, 200 mM ATP, 200 mM CTP, 200 mM GTP, 10 mM UTP, and 2.5 mCi [α-32P]UTP in transcription buffer. After 10 min at 30°C, reactions were stopped by the addition of stop solution (80% formamide, 20 mM EDTA, 0.1% xylene cyanol, and 0.1% bromophenol blue). Samples were electrophoresed on 6% polyacrylamide gels containing 7.5 M urea, and transcripts were visualized by phosphorimaging. The linear Pfam template was generated by amplification of the promoter region of the gene encoding σ32 in RB50, fam, using the primers PFamF and PFamR (Table 2). The sequence logo in Figure 1C was generated using WebLogo version 2.8.2 (http://WebLogo.berkeley.edu, ).
Disk diffusion assays
B. bronchiseptica cultures in mid-log phase were diluted to 6 × 108 CFU/ml and spread on Stainer-Scholte agar plates to generate a lawn of bacteria. Disks containing 300 IU polymyxin B, 10 μg ampicillin, 100 μg mecillinam, 750 μg sodium dodecyl sulfate (SDS) and 2.9 μg EDTA, 30 μg aztreonam, 10 μg imipenem, 10 μg meropenem, 30 μg chloramphenicol, 15 μg erythromycin, 30 μg kanamycin, 30 μg nalidixic acid, 150 μg rifampicin, 23.75 μg sulfamethoxazole and 1.25 μg trimethoprim, 30 μg tetracycline, 3.0 μg deoxycholate, 3% hydrogen peroxide, or 2% paraquat were applied to the plates and the zones of inhibition were measured after overnight incubation at 37°C.
Temperature and ethanol stress
For temperature stress experiments, mid-log phase cultures of RB50 and RB50ΔsigE were diluted to an OD600 of 0.01 in fresh Stainer-Scholte broth and incubated at 37°C in a gyratory water bath with shaking. At an OD600 of 0.1, cultures were either shifted to 40°C for adaptation or kept at 37°C. After 90 minutes, all cultures were shifted to 50°C, and survival was measured by plating and CFU counts. For ethanol stress experiments, mid-log-phase cultures of the pertinent strains were subcultured into fresh Stainer-Scholte broth with or without 3% ethanol and incubated at 37°C in a gyratory water bath with aeration. Bacterial growth was measured by OD600.
Complement killing assay
Complement killing assays were performed as previously described . Approximately 500 CFU of RB50, RB50ΔsigE, and RB50Δwbm from mid-log phase cultures were incubated with 45 μl of diluted serum from C57BL/6 mice or PBS (final volume for incubation was 50 μl) for 1 hour at 37°C. Bacterial numbers before and after incubation were determined by plating and CFU counts. Each strain was assayed in triplicate.
Cytotoxicity assays were performed as previously described . Briefly, bacteria were added to RAW 264.7 murine macrophage cells at a multiplicity of infection (MOI) of 10 and incubated for four hours. Percent lactate dehydrogenase (LDH) release, a measure of cytotoxicity, was determined by using Cytotox96 Kit (Promega) according to the manufacturer’s protocol.
Phagocytosis and killing by polymorphonuclear leukocytes
Attachment and phagocytosis of the B. bronchiseptica strains by peripheral blood polymorphonuclear leukocytes (PMNs) were evaluated as previously described with a few modifications . Briefly, GFP-expressing bacteria were incubated with PMNs at an MOI of 50 for 20 min at 37°C to allow binding. After extensive washing to remove non-attached bacteria, an aliquot was maintained on ice to be used as a bacterial attachment control. The remaining PMNs were further incubated for 30 min at 37°C to allow internalization, at which point phagocytosis was stopped by placing PMNs on ice. Bacteria bound to the cell surface in both aliquots were detected by incubation with RB50 immune serum for 30 min at 4°C, followed by incubation with R-phycoerythrin (RPE)–labeled goat F(ab')2 fragments of anti-mouse IgG at 4°C for 30 min. All incubations were done in the presence of 25% heat-inactivated human serum to prevent nonspecific binding of antibodies. After washing, ten thousand cells per sample were analyzed by flow cytometry. Attachment control samples were also analyzed by fluorescence microscopy using a DMLB microscope coupled to a DC 100 camera (Leica Microscopy Systems Ltd.). Green fluorescence intensity associated with PMNs maintained at 37°C for 20 min has previously been shown to represent bacterial attachment . Phagocytosis was calculated from the decrease in mean red fluorescence intensity of GFP-positive PMNs after the 30 min incubation allowing for internalization, as previously described . Percent phagocytosis was calculated as follows: 100 × (1-RPE2/RPE1), where RPE1 is the mean RPE-fluorescence of the GFP-positive cells after 20 min at 37°C (attachment control) and RPE2 is the mean RPE-fluorescence of the GFP-positive cells after 50 min (internalized bacteria) at 37°C.
Killing of bacteria by PMNs was assessed as follows: after phagocytosis of the bacteria, 400 μg/ml polymyxin B and 350 μg/ml chloramphenicol were added to the PMNs for 1 hour to kill the remaining extracellular bacteria and assess intracellular survival. Serial dilutions of samples were plated to determine the number of viable intracellular bacteria per PMN. The relative percent survival of internalized bacteria was calculated from the relative phagocytosis index and taking into account the initial attachment level of each strain, as follows: percent bacterial killing = [1-N/(A × P)] × 100, where A = number of bacteria associated with PMN after 20 min at 37°C (determined by fluorescent microscopy), P = phagocytosis index (1-RPE2/RPE1), N = number of viable bacteria per cell after incubation with antibiotics. Control experiments to assess the efficacy of antibiotic bactericidal activity were performed in parallel. Briefly, samples of 5 × 108 bacteria were incubated with antibiotics for 30 min at 37°C and plated. This resulted in a >99% decrease in CFU.
C57BL/6J, B6.129 S-Tnftm1Gkl/J (TNF-α−/−), B6 129S7-Rag1tm1Mom/J (Rag1−/−), C3H/HeOuJ (TLR4suf) and C3H/HeJ (TLR4def) mice were obtained from Jackson laboratories (Bar Harbor). All mice were bred in our Bordetella-free, specific pathogen-free breeding rooms at The Pennsylvania State University. For inoculation, mice were sedated with 5% isoflurane (Abbott laboratory) in oxygen and 50 μl of PBS containing 105 or 5 × 105 CFU of the indicated bacteria were pipeted onto the external nares [76, 77]. This method reliably distributes the bacteria throughout the respiratory tract . Survival curves were generated by inoculating TLR4def, TNF-α−/− and Rag1−/− mice with either RB50 or RB50ΔsigE. Mice suffering from lethal bordetellosis as determined by severe hunched posture, ruffled fur, extremely labored breathing and apathy were euthanized to prevent unnecessary suffering . For quantifying bacterial load, mice were euthanized via CO2 inhalation, and lung, trachea, nasal cavity, spleen, liver and/or kidneys were excised. Tissues were homogenized in PBS, aliquots were serially diluted, plated, incubated at 37°C for 2 to 3 days, and CFU were determined. All protocols were reviewed by the university IACUC and all animals were handled in accordance with institutional guidelines (IACUC approval number: 31297).
The mean +/− standard error (SE) of the geometric mean was determined when appropriate and expressed as error bars. Two-tailed, unpaired Student’s T-tests were used to determine statistical significance between groups. All experiments were performed at least twice with similar results.
We thank Dr. Scott Stibitz (FDA) for providing the allelic exchange vector pSS3962 and the helper plasmid pSS1827. We thank Dr. Kenneth Keiler (the Pennsylvania State University) for providing the plasmid pJS72. This work was supported by NIH grant GM083113 (E.T.H), in part by NSF grant MCB-0347302 (S.E.A.) and a NSF Graduate Research Fellowship to S.E.B.
- MacRitchie DM, Buelow DR, Price NL, Raivio TL: Two-component signaling and gram negative envelope stress response systems. Adv Exp Med Biol. 2008, 631: 80-110. 10.1007/978-0-387-78885-2_6.PubMedView ArticleGoogle Scholar
- Rowley G, Spector M, Kormanec J, Roberts M: Pushing the envelope: extracytoplasmic stress responses in bacterial pathogens. Nat Rev Microbiol. 2006, 4: 383-394. 10.1038/nrmicro1394.PubMedView ArticleGoogle Scholar
- Crouch ML, Becker LA, Bang IS, Tanabe H, Ouellette AJ, Fang FC: The alternative sigma factor sigma is required for resistance of Salmonella enterica serovar Typhimurium to anti-microbial peptides. Mol Microbiol. 2005, 56: 789-799. 10.1111/j.1365-2958.2005.04578.x.PubMedView ArticleGoogle Scholar
- Ernst RK, Guina T, Miller SI: Salmonella Typhimurium outer membrane remodeling: role in resistance to host innate immunity. Microb Infect. 2001, 3: 1327-1334. 10.1016/S1286-4579(01)01494-0.View ArticleGoogle Scholar
- Jongerius I, Ram S, Rooijakkers S: Bacterial complement escape. Adv Exp Med Biol. 2009, 666: 32-48. 10.1007/978-1-4419-1601-3_3.PubMedView ArticleGoogle Scholar
- Humphreys S, Stevenson A, Bacon A, Weinhardt AB, Roberts M: The alternative sigma factor, σE, is critically important for the virulence of Salmonella Typhimurium. Infect Immun. 1999, 67: 1560-1568.PubMedPubMed CentralGoogle Scholar
- Mathur J, Waldor MK: The Vibrio cholerae ToxR-regulated porin OmpU confers resistance to antimicrobial peptides. Infect Immun. 2004, 72: 3577-3583. 10.1128/IAI.72.6.3577-3583.2004.PubMedPubMed CentralView ArticleGoogle Scholar
- Raivio TL: Envelope stress responses and Gram-negative bacterial pathogenesis. Mol Microbiol. 2005, 56: 1119-1128. 10.1111/j.1365-2958.2005.04625.x.PubMedView ArticleGoogle Scholar
- Arico B, Gross R, Smida J, Rappuoli R: Evolutionary relationships in the genus Bordetella. Mol Microbiol. 1987, 1: 301-308. 10.1111/j.1365-2958.1987.tb01936.x.PubMedView ArticleGoogle Scholar
- Parkhill J, Sebaihia M, Preston A, Murphy LD, Thomson N, Harris DE, Holden MT, Churcher CM, Bentley SD, Mungall KL, et al: Comparative analysis of the genome sequences of Bordetella pertussis, Bordetella parapertussis and Bordetella bronchiseptica. Nat Genet. 2003, 35: 32-40. 10.1038/ng1227.PubMedView ArticleGoogle Scholar
- Goodnow RA: Biology of Bordetella bronchiseptica. Microbiol Rev. 1980, 44: 722-738.PubMedPubMed CentralGoogle Scholar
- Mattoo S, Cherry JD: Molecular pathogenesis, epidemiology, and clinical manifestations of respiratory infections due to Bordetella pertussis and other Bordetella subspecies. Clin Microbiol Rev. 2005, 18: 326-382. 10.1128/CMR.18.2.326-382.2005.PubMedPubMed CentralView ArticleGoogle Scholar
- Musser JM, Bemis DA, Ishikawa H, Selander RK: Clonal diversity and host distribution in Bordetella bronchiseptica. J Bacteriol. 1987, 169: 2793-2803.PubMedPubMed CentralGoogle Scholar
- Mazumder SA, Cleveland KO: Bordetella bronchiseptica bacteremia in a patient with AIDS. South Med J. 2010, 103: 934-935. 10.1097/SMJ.0b013e3181ebcdbc.PubMedView ArticleGoogle Scholar
- Madan Babu M, Teichmann SA, Aravind L: Evolutionary dynamics of prokaryotic transcriptional regulatory networks. J Mol Biol. 2006, 358: 614-633. 10.1016/j.jmb.2006.02.019.PubMedView ArticleGoogle Scholar
- Brickman TJ, Vanderpool CK, Armstrong SK: Heme transport contributes to in vivo fitness of Bordetella pertussis during primary infection in mice. Infect Immun. 2006, 74: 1741-1744. 10.1128/IAI.74.3.1741-1744.2006.PubMedPubMed CentralView ArticleGoogle Scholar
- Conover MS, Redfern CJ, Ganguly T, Sukumar N, Sloan G, Mishra M, Deora R: BpsR modulates Bordetella biofilm formation by negatively regulating the expression of the Bps polysaccharide. J Bacteriol. 2012, 194: 233-242. 10.1128/JB.06020-11.PubMedPubMed CentralView ArticleGoogle Scholar
- Jungnitz H, West NP, Walker MJ, Chhatwal GS, Guzman CA: A second two-component regulatory system of Bordetella bronchiseptica required for bacterial resistance to oxidative stress, production of acid phosphatase, and in vivo persistence. Infect Immun. 1998, 66: 4640-4650.PubMedPubMed CentralGoogle Scholar
- Vanderpool CK, Armstrong SK: Integration of environmental signals controls expression of Bordetella heme utilization genes. J Bacteriol. 2004, 186: 938-948. 10.1128/JB.186.4.938-948.2004.PubMedPubMed CentralView ArticleGoogle Scholar
- Zimna K, Medina E, Jungnitz H, Guzman CA: Role played by the response regulator Ris in Bordetella bronchiseptica resistance to macrophage killing. FEMS Microbiol Lett. 2001, 201: 177-180. 10.1111/j.1574-6968.2001.tb10753.x.PubMedView ArticleGoogle Scholar
- Paget MS, Helmann JD: The sigma70 family of sigma factors. Genome Biol. 2003, 4: 203-10.1186/gb-2003-4-1-203.PubMedPubMed CentralView ArticleGoogle Scholar
- Gruber TM, Gross CA: Multiple sigma subunits and the partitioning of bacterial transcription space. Annu Rev Microbiol. 2003, 57: 441-466. 10.1146/annurev.micro.57.030502.090913.PubMedView ArticleGoogle Scholar
- Helmann JD: The extracytoplasmic function (ECF) sigma factors. Adv Microb Physiol. 2002, 46: 47-110.PubMedView ArticleGoogle Scholar
- Staron A, Sofia HJ, Dietrich S, Ulrich LE, Liesegang H, Mascher T: The third pillar of bacterial signal transduction: classification of the extracytoplasmic function (ECF) sigma factor protein family. Mol Microbiol. 2009, 74: 557-581. 10.1111/j.1365-2958.2009.06870.x.PubMedView ArticleGoogle Scholar
- Missiakas D, Raina S: The extracytoplasmic function sigma factors: role and regulation. Mol Microbiol. 1998, 28: 1059-1066. 10.1046/j.1365-2958.1998.00865.x.PubMedView ArticleGoogle Scholar
- Alba BM, Gross CA: Regulation of the Escherichia coli sigma-dependent envelope stress response. Mol Microbiol. 2004, 52: 613-619. 10.1111/j.1365-2958.2003.03982.x.PubMedView ArticleGoogle Scholar
- Rhodius VA, Suh WC, Nonaka G, West J, Gross CA: Conserved and variable functions of the σE stress response in related genomes. PLoS Biol. 2006, 4: e2-10.1371/journal.pbio.0040002.PubMedPubMed CentralView ArticleGoogle Scholar
- Muller C, Bang IS, Velayudhan J, Karlinsey J, Papenfort K, Vogel J, Fang FC: Acid stress activation of the σE stress response in Salmonella enterica serovar Typhimurium. Mol Microbiol. 2009, 71: 1228-1238. 10.1111/j.1365-2958.2009.06597.x.PubMedPubMed CentralView ArticleGoogle Scholar
- Testerman TL, Vazquez-Torres A, Xu Y, Jones-Carson J, Libby SJ, Fang FC: The alternative sigma factor σE controls antioxidant defences required for Salmonella virulence and stationary-phase survival. Mol Microbiol. 2002, 43: 771-782. 10.1046/j.1365-2958.2002.02787.x.PubMedView ArticleGoogle Scholar
- Deretic V, Schurr MJ, Boucher JC, Martin DW: Conversion of Pseudomonas aeruginosa to mucoidy in cystic fibrosis: environmental stress and regulation of bacterial virulence by alternative sigma factors. J Bacteriol. 1994, 176: 2773-2780.PubMedPubMed CentralGoogle Scholar
- Rowen DW, Deretic V: Membrane-to-cytosol redistribution of ECF sigma factor AlgU and conversion to mucoidy in Pseudomonas aeruginosa isolates from cystic fibrosis patients. Mol Microbiol. 2000, 36: 314-327. 10.1046/j.1365-2958.2000.01830.x.PubMedView ArticleGoogle Scholar
- De Las Penas A, Connolly L, Gross CA: The σE-mediated response to extracytoplasmic stress in Escherichia coli is transduced by RseA and RseB, two negative regulators of σE. Mol Microbiol. 1997, 24: 373-385. 10.1046/j.1365-2958.1997.3611718.x.PubMedView ArticleGoogle Scholar
- Heusipp G, Schmidt MA, Miller VL: Identification of rpoE and nadB as host responsive elements of Yersinia enterocolitica. FEMS Microbiol Lett. 2003, 226: 291-298. 10.1016/S0378-1097(03)00613-X.PubMedView ArticleGoogle Scholar
- Mecsas J, Rouviere PE, Erickson JW, Donohue TJ, Gross CA: The activity of σE, an Escherichia coli heat-inducible sigma-factor, is modulated by expression of outer membrane proteins. Genes Dev. 1993, 7: 2618-2628. 10.1101/gad.7.12b.2618.PubMedView ArticleGoogle Scholar
- De Las Penas A, Connolly L, Gross CA: σE is an essential sigma factor in Escherichia coli. J Bacteriol. 1997, 179: 6862-6864.PubMedPubMed CentralGoogle Scholar
- Flannagan RS, Valvano MA: Burkholderia cenocepacia requires RpoE for growth under stress conditions and delay of phagolysosomal fusion in macrophages. Microbiology. 2008, 154: 643-653. 10.1099/mic.0.2007/013714-0.PubMedView ArticleGoogle Scholar
- Yu H, Schurr MJ, Deretic V: Functional equivalence of Escherichia coli σE and Pseudomonas aeruginosa AlgU: E. coli rpoE restores mucoidy and reduces sensitivity to reactive oxygen intermediates in algU mutants of P. aeruginosa. J Bacteriol. 1995, 177: 3259-3268.PubMedPubMed CentralGoogle Scholar
- Bianchi AA, Baneyx F: Hyperosmotic shock induces the σ32 and σE stress regulons of Escherichia coli. Mol Microbiol. 1999, 34: 1029-1038. 10.1046/j.1365-2958.1999.01664.x.PubMedView ArticleGoogle Scholar
- Mathur J, Davis BM, Waldor MK: Antimicrobial peptides activate the Vibrio cholerae σE regulon through an OmpU-dependent signalling pathway. Mol Microbiol. 2007, 63: 848-858.PubMedView ArticleGoogle Scholar
- Keith LM, Bender CL: AlgT (σ22) controls alginate production and tolerance to environmental stress in Pseudomonas syringae. J Bacteriol. 1999, 181: 7176-7184.PubMedPubMed CentralGoogle Scholar
- Korbsrisate S, Vanaporn M, Kerdsuk P, Kespichayawattana W, Vattanaviboon P, Kiatpapan P, Lertmemongkolchai G: The Burkholderia pseudomallei RpoE (AlgU) operon is involved in environmental stress tolerance and biofilm formation. FEMS Microbiol Lett. 2005, 252: 243-249. 10.1016/j.femsle.2005.09.002.PubMedView ArticleGoogle Scholar
- Tomoyasu T, Mogk A, Langen H, Goloubinoff P, Bukau B: Genetic dissection of the roles of chaperones and proteases in protein folding and degradation in the Escherichia coli cytosol. Mol Microbiol. 2001, 40: 397-413. 10.1046/j.1365-2958.2001.02383.x.PubMedView ArticleGoogle Scholar
- Kovacikova G, Skorupski K: The alternative sigma factor σE plays an important role in intestinal survival and virulence in Vibrio cholerae. Infect Immun. 2002, 70: 5355-5362. 10.1128/IAI.70.10.5355-5362.2002.PubMedPubMed CentralView ArticleGoogle Scholar
- Harvill ET, Cotter PA, Yuk MH, Miller JF: Probing the function of Bordetella bronchiseptica adenylate cyclase toxin by manipulating host immunity. Infect Immun. 1999, 67: 1493-1500.PubMedPubMed CentralGoogle Scholar
- Mann PB, Elder KD, Kennett MJ, Harvill ET: Toll-like receptor 4-dependent early elicited tumor necrosis factor alpha expression is critical for innate host defense against Bordetella bronchiseptica. Infect Immun. 2004, 72: 6650-6658. 10.1128/IAI.72.11.6650-6658.2004.PubMedPubMed CentralView ArticleGoogle Scholar
- Mann PB, Kennett MJ, Harvill ET: Toll-like receptor 4 is critical to innate host defense in a murine model of bordetellosis. J Infect Dis. 2004, 189: 833-836. 10.1086/381898.PubMedView ArticleGoogle Scholar
- Mann PB, Wolfe D, Latz E, Golenbock D, Preston A, Harvill ET: Comparative toll-like receptor 4-mediated innate host defense to Bordetella infection. Infect Immun. 2005, 73: 8144-8152. 10.1128/IAI.73.12.8144-8152.2005.PubMedPubMed CentralView ArticleGoogle Scholar
- Burns VC, Pishko EJ, Preston A, Maskell DJ, Harvill ET: Role of Bordetella O antigen in respiratory tract infection. Infect Immun. 2003, 71: 86-94. 10.1128/IAI.71.1.86-94.2003.PubMedPubMed CentralView ArticleGoogle Scholar
- Yuk MH, Harvill ET, Miller JF: The BvgAS virulence control system regulates type III secretion in Bordetella bronchiseptica. Mol Microbiol. 1998, 28: 945-959. 10.1046/j.1365-2958.1998.00850.x.PubMedView ArticleGoogle Scholar
- Bock A, Gross R: The BvgAS two-component system of Bordetella spp.: a versatile modulator of virulence gene expression. Int J Med Microb. 2001, 291: 119-130. 10.1078/1438-4221-00109.View ArticleGoogle Scholar
- Cotter PA, Jones AM: Phosphorelay control of virulence gene expression in Bordetella. Trends Microbiol. 2003, 11: 367-373. 10.1016/S0966-842X(03)00156-2.PubMedView ArticleGoogle Scholar
- Mattoo S, Foreman-Wykert AK, Cotter PA, Miller JF: Mechanisms of Bordetella pathogenesis. Front Biosci. 2001, 6: E168-E186. 10.2741/Mattoo.PubMedView ArticleGoogle Scholar
- Bashyam MD, Hasnain SE: The extracytoplasmic function sigma factors: role in bacterial pathogenesis. Infect Genet Evol. 2004, 4: 301-308. 10.1016/j.meegid.2004.04.003.PubMedView ArticleGoogle Scholar
- Gerlach G, von Wintzingerode F, Middendorf B, Gross R: Evolutionary trends in the genus Bordetella. Microb Infect. 2001, 3: 61-72. 10.1016/S1286-4579(00)01353-8.View ArticleGoogle Scholar
- Porter JF, Parton R, Wardlaw AC: Growth and survival of Bordetella bronchiseptica in natural waters and in buffered saline without added nutrients. Appl Environ Microbiol. 1991, 57: 1202-1206.PubMedPubMed CentralGoogle Scholar
- Park SD, Youn JW, Kim YJ, Lee SM, Kim Y, Lee HS: Corynebacterium glutamicum σE is involved in responses to cell surface stresses and its activity is controlled by the anti-sigma factor CseE. Microbiology. 2008, 154: 915-923. 10.1099/mic.0.2007/012690-0.PubMedView ArticleGoogle Scholar
- Sheehan BJ, Bosse JT, Beddek AJ, Rycroft AN, Kroll JS, Langford PR: Identification of Actinobacillus pleuropneumoniae genes important for survival during infection in its natural host. Infect Immun. 2003, 71: 3960-3970. 10.1128/IAI.71.7.3960-3970.2003.PubMedPubMed CentralView ArticleGoogle Scholar
- Cotter PA, Miller JF: BvgAS-mediated signal transduction: analysis of phase-locked regulatory mutants of Bordetella bronchiseptica in a rabbit model. Infect Immun. 1994, 62: 3381-3390.PubMedPubMed CentralGoogle Scholar
- Stainer DW, Scholte MJ: A simple chemically defined medium for the production of phase I Bordetella pertussis. J Gen Microbiol. 1970, 63: 211-220. 10.1099/00221287-63-2-211.PubMedView ArticleGoogle Scholar
- Costanzo A, Ades SE: Growth phase-dependent regulation of the extracytoplasmic stress factor, σE, by guanosine 3',5'-bispyrophosphate (ppGpp). J Bacteriol. 2006, 188: 4627-4634. 10.1128/JB.01981-05.PubMedPubMed CentralView ArticleGoogle Scholar
- Costanzo A, Nicoloff H, Barchinger SE, Banta AB, Gourse RL, Ades SE: ppGpp and DksA likely regulate the activity of the extracytoplasmic stress factor σE in Escherichia coli by both direct and indirect mechanisms. Mol Microbiol. 2008, 67: 619-632. 10.1111/j.1365-2958.2007.06072.x.PubMedView ArticleGoogle Scholar
- Hayden JD, Ades SE: The Extracytoplasmic stress factor, σE, is required to maintain cell envelope integrity in Escherichia coli. PLoS One. 2008, 3: e1573-10.1371/journal.pone.0001573.PubMedPubMed CentralView ArticleGoogle Scholar
- Stibitz S, Aaronson W, Monack D, Falkow S: The vir locus and phase-variation in Bordetella pertussis. Tokai J Exp Clin Med. 1988, 13 (Suppl): 223-226.PubMedGoogle Scholar
- Preston A, Allen AG, Cadisch J, Thomas R, Stevens K, Churcher CM, Badcock KL, Parkhill J, Barrell B, Maskell DJ: Genetic basis for lipopolysaccharide O-antigen biosynthesis in bordetellae. Infect Immun. 1999, 67: 3763-3767.PubMedPubMed CentralGoogle Scholar
- Rouviere PE, De Las Penas A, Mecsas J, Lu CZ, Rudd KE, Gross CA: rpoE, the gene encoding the second heat-shock sigma factor, σE, in Escherichia coli. EMBO J. 1995, 14: 1032-1042.PubMedPubMed CentralGoogle Scholar
- Schaeffer LM, McCormack FX, Wu H, Weiss AA: Bordetella pertussis lipopolysaccharide resists the bactericidal effects of pulmonary surfactant protein A. J Immunol. 2004, 173: 1959-1965.PubMedView ArticleGoogle Scholar
- Datsenko KA, Wanner BL: One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci U S A. 2000, 97: 6640-6645. 10.1073/pnas.120163297.PubMedPubMed CentralView ArticleGoogle Scholar
- Weingart CL, Broitman-Maduro G, Dean G, Newman S, Peppler M, Weiss AA: Fluorescent labels influence phagocytosis of Bordetella pertussis by human neutrophils. Infect Immun. 1999, 67: 4264-4267.PubMedPubMed CentralGoogle Scholar
- Buboltz AM, Nicholson TL, Weyrich LS, Harvill ET: Role of the type III secretion system in a hypervirulent lineage of Bordetella bronchiseptica. Infect Immun. 2009, 77: 3969-3977. 10.1128/IAI.01362-08.PubMedPubMed CentralView ArticleGoogle Scholar
- Stibitz S, Carbonetti NH: Hfr mapping of mutations in Bordetella pertussis that define a genetic locus involved in virulence gene regulation. J Bacteriol. 1994, 176: 7260-7266.PubMedPubMed CentralGoogle Scholar
- Miller JH: Experiments in molecular genetics. 1972, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NYGoogle Scholar
- Crooks GE, Hon G, Chandonia JM, Brenner SE: WebLogo: a sequence logo generator. Genome Res. 2004, 14: 1188-1190. 10.1101/gr.849004.PubMedPubMed CentralView ArticleGoogle Scholar
- Goebel EM, Wolfe DN, Elder K, Stibitz S, Harvill ET: O-antigen protects Bordetella parapertussis from complement. Infect Immun. 2008, 76: 1774-1780. 10.1128/IAI.01629-07.PubMedPubMed CentralView ArticleGoogle Scholar
- Rodriguez ME, Hellwig SM, Hozbor DF, Leusen J, van der Pol WL, van de Winkel JG: Fc receptor-mediated immunity against Bordetella pertussis. J Immunol. 2001, 167: 6545-6551.PubMedView ArticleGoogle Scholar
- Rodriguez ME, Van der Pol WL, Van de Winkel JG: Flow cytometry-based phagocytosis assay for sensitive detection of opsonic activity of pneumococcal capsular polysaccharide antibodies in human sera. J Immunol Methods. 2001, 252: 33-44. 10.1016/S0022-1759(01)00329-5.PubMedView ArticleGoogle Scholar
- Harvill ET, Preston A, Cotter PA, Allen AG, Maskell DJ, Miller JF: Multiple roles for Bordetella lipopolysaccharide molecules during respiratory tract infection. Infect Immun. 2000, 68: 6720-6728. 10.1128/IAI.68.12.6720-6728.2000.PubMedPubMed CentralView ArticleGoogle Scholar
- Kirimanjeswara GS, Agosto LM, Kennet MJ, Bjornstad ON, Harvill ET: Pertussis toxin inhibits neutrophil recruitment to inhibit antibody-mediated clearance of Bordetella pertussis. J Clin Invest. 2005, 115: 3594-3601. 10.1172/JCI24609.PubMedPubMed CentralView ArticleGoogle Scholar
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