YqiC of Salmonella enterica serovar Typhimurium is a membrane fusogenic protein required for mice colonization
© Carrica et al; licensee BioMed Central Ltd. 2011
Received: 24 January 2011
Accepted: 9 May 2011
Published: 9 May 2011
Salmonella enterica serovar Typhimurium is an intracellular bacterial pathogen which can colonize a variety of hosts, including human, causing syndromes that vary from gastroenteritis and diarrhea to systemic disease.
In this work we present structural information as well as insights into the in vivo function of YqiC, a 99-residue protein of S. Typhimurium, which belongs to the cluster of the orthologous group 2960 (COG2960). We found that YqiC shares biophysical and biochemical properties with Brucella abortus BMFP, the only previously characterized member of this group, such as a high alpha helix content, a coiled-coil domain involved in trimerization and a membrane fusogenic activity in vitro. In addition, we demonstrated that YqiC localizes at cytoplasmic and membrane subcellular fractions, that a S. Typhimurium yqiC deficient strain had a severe attenuation in virulence in the murine model when inoculated both orally and intraperitoneally, and was impaired to replicate at physiological and high temperatures in vitro, although it was still able to invade and replicate inside epithelial and macrophages cell lines.
This work firstly demonstrates the importance of a COG2960 member for pathogen-host interaction, and suggests a common function conserved among members of this group.
Salmonella enterica is an intracellular facultative anaerobe Gram-negative that infects a variety of hosts, which include mammals, avians and reptiles. In human beings, S. enterica causes over 33 million cases of disease worldwide annually, which may vary from gastroenteritis and diarrhea to severe life-threatening systemic disease (typhoid fever) . The outcome of the disease depends on both the serovar of Samonella and the host susceptibility. Salmonella enterica serovar Typhimurium (S. Typhimurium), can infect humans and animals, but causes different syndromes in each host. In humans, Salmonella produces enterocolitis, but in mice it causes a systemic illness that resembles human typhoid fever. Because of this, S. Typhimurium is widely used as a model organism to study the host-pathogen interactions that contribute to the onset of the systemic disease [2, 3].
The pathogenic strategy of S. Typhimurium includes penetration of the mucosal barrier, invasion of non-phagocytic cells of the intestinal mucosa and survival and replication inside macrophages of the spleen and liver during the systemic phase. The ability of S. Typhimurium to survive to host defense mechanisms and to cause disease has been directly linked to genes encoded in pathogenicity islands, which are large horizontally acquired regions of the chromosome. Of outstanding importance are two type three secretion systems (T3SS), which together with cognate effector proteins are essential for cell invasion, intracellular survival and, therefore, for mice colonization . Besides, factors encoded in the genomic backbone of Salmonella are also important for virulence in the murine model [5–8].
YqiC is a 99-residue protein of S. Typhimurium (UniProtKB entry K09806, gene STM 3196) which belongs to the cluster of orthologous groups 2960 (COG 2960). This COG includes 322 members (Pfam June 2010), encoded in genomes of pathogenic, non-pathogenic and symbiotic bacteria. In spite of the high conservation of this COG across bacterial species, no description of the in vivo function of any member has been reported. In this work, we carried out microbiological studies which demonstrate that YqiC is required for the pathogenesis of S. Typhimurium in the murine model, since a null mutant is highly attenuated when inoculated both orally and intraperitoneally. We also show that this protein is dispensable for cell invasion and intracellular replication in murine macrophages and human epithelial cell lines, but it is necessary for efficient growth at the mammalian host physiological temperature outside the cells. The microbiological results are complemented by biophysical and biochemical studies. These analyses demonstrate that YqiC shares properties with the recently reported BMFP from Brucella abortus (another member of the COG 2960) which include a trimeric coiled-coil structure and the ability to induce membrane fusion in vitro. The results presented here contribute to elucidate the function of members of the COG 2960 and their biological role.
S. Typhimurium YqiC is a trimeric protein with a high helical content
The characteristics described here are similar to the structural features that we have previously reported for Brucella abortus BMFP, which is a member of the COG 2960 that only conserves 22% sequence identity with YqiC .
YiqC promotes membrane fusion in vitro
Subcellular localization of YqiC
Evaluation of a yqiC defective strain phenotype in vitro
Survival of the STM-yqiC mutant in cultured cells
Role of S. Typhimurim YqiC in virulence
Determination of LD50 of S.Typhimurium strains in mice.
Number of dead mice/Number of infected mice
(Mean of days to death)
S . Typhimurium ATCC 14028
S . Typhimurium 14028 Δ yqiC ::CAT
1 × 101
1 × 102
1 × 103
1 × 104
1 × 105
In this work we have characterized the YqiC protein of S. Typhimurium. YqiC shares common structural and biochemical characteristics with its previously reported orthologous BMFP protein of Brucella abortus, although these proteins share only 22% of sequence identity and Brucella spp and Salmonella are phylogenetically distant bacteria. The common structural characteristics between YqiC and BMFP, namely high alpha helix content, coiled coil C-terminal and amphipathic alpha helix N-terminus, are also predicted by bioinformatics analysis for other proteins of the COG 2960 (such as those encoded by Escherichia coli, Shewanella oneidensis, Legionella pneumophila, Xanthomonas campestris, Pseudomonas aeruginosa, Bordetella pertussis, Agrobacterium tumefaciens, Sinorhizobium meliloti and Rhodopseudomonas palustris). This structural conservation strongly suggests a common function for the members of this COG.
In addition, we demonstrated that YqiC has membrane fusogenic activity, like BMFP and other trimeric coiled-coil and/or amphipathic proteins [11, 12]. This activity is higher at acidic pH. A similar fusogenic activity at low pH was observed for B. abortus BMFP (unpublished data). The fusogenic activity could be relevant as many processes that involve bacterial or host cell membrane fusion events are important for pathogenic bacteria to successfully establish host infection. In this regard, both S. Typhimurium and B. abortus penetrate the cell by phagocytosis, and reside within a host vacuole that rapidly undergoes acidification. This drop in the pH serves as a signal for the expression of bacterial factors that alter intracellular membrane traffic in order to set their replicative niche [13–15]. The improved YqiC activity at low pH could indicate that this protein is active at the vacuolar stage of the bacterial infection. It is interesting to highlight that YqiC shares structural similarity with S. Typhimurium-SipB protein, as both are predominantly alpha helical in aqueous solution and have a coiled-coil domain involved in trimerization . SipB is an effector protein essential for Salmonella invasion secreted through the SPI-1-encoded T3SS and was the first bacterial protein reported to display membrane fusogenic activity , however the function of this membrane fusogenic activity in the bacterial pathogenesis has not been clearly defined . The activity of YqiC may be required during the interaction of Salmonella with the host cell to hijack membrane trafficking pathways. This would probably be accomplished by competitive inhibition, mimicking eukaryotic membrane fusogenic proteins, such as the SNAREs (given the structural similitude with these proteins) and inhibiting lysosomal fusion with the Salmonella-containing vacuoles. Current work is addressing whether YqiC is translocated to the host cell.
Alternatively, the YqiC-membrane fusogenic activity could be required during the biogenesis of bacterial outer membrane vesicles (OMV), which are spherical bilayered structures liberated from the outer membrane in Gram negative bacteria . OMV act as delivery vesicles for bacterial toxins into host cells, promote quorum sensing, are involved in stress response, inhibit phagosome-lysosome fusion during bacterial growth within macrophages and are important constituents of the matrix of Gram-negative and mixed bacterial biofilm [19–23]. To date, the machinery that cause vesicle formation remains elusive but it may be expected that a protein with membrane fusion activity could be involved in this process [18, 24]. In this regard, in spite of the lack of a signal peptide or transmembrane domains we demonstrated that YqiC can be localized both soluble and associated to membranes. This localization pattern was also observed for B. abortus BMFP (unpublished data). Subcellular localization pattern of YqiC may be in tune with its hypothetical function in biogenesis of OMV, as soluble and membrane-bound states of YqiC can be related to transient associations of this protein with the outer membrane. At this point, is interesting to note that OMV produced by Shigella flexneri contain IpaB, a SipB homologue which also displays membrane fusion activity [25, 26]. Accordingly, many of the bacterial species conserving an YqiC homolog have been shown to generate OMV [18, 27]. Further work is needed to investigate the possible role of YqiC in the biogenesis of OMV.
The in vivo importance of YqiC was demonstrated by the fact that this protein is necessary for S. Typhimurium virulence in the murine model, as an yqiC mutant strain was unable to kill mice within the period of time assayed and had a significantly higher LD50. The basis for this attenuation in virulence may be related to the observed defect to grow at physiological temperature in vitro. Temperature represents a common environmental challenge that microorganisms should be able to sense and respond to in order to survive . Many other single gene mutations produce temperature-sensitive, virulence-attenuated Salmonella strains. Examples include smpA, which encodes for an outer membrane lipoprotein, uspA, which encodes for an universal stress response protein and the genes for DegP and DegQ proteases [29–31]. Interestingly, temperature sensitivity could not be the only factor responsible for the virulence attenuation observed for the yqiC mutant, as this strain was still able to invade and replicate inside macrophages and epithelial cell lines incubated at 37°C. These phenotypes may be due to differences in the metabolic status and environmental conditions affecting bacteria replication in rich media under laboratory conditions and inside the eukaryotic cell.
We have demonstrated in this work that S. Typhimurium YqiC shares structural and biochemical characteristics with B. abortus BMFP, in spite of their relatively low sequence identity. Thus, members of the COG 2960 may accomplish a conserved function among phylogenetically distant bacteria. This function may be necessary to display full virulence. This seems to be the case, as in a parallel work we observed virulence attenuation when analyzing a B. abortus BMFP-defective strain (Cravero et al, unpublished work) . This work is the first demonstration of the in vivo importance of a member of the COG 2960. However, future research is necessary to clarify the physiological processes in which the membrane fusogenic activity and possibly other unknown functions of YqiC are required.
All experiments involving animals have been approved by the ethics committee of the Instituto Nacional de Tecnologia Agropecuaria (INTA) where they were conducted. This ethics committee works according with the National Institutes of Health Guide for the Care and Use of Animals Laboratory .
Bacterial Strains and Growth Conditions
For this study, we used the WT Salmonella enterica serovar Typhimurium strain ATCC 14028. Bacterial strains were grown in Luria-Bertani (LB) or M9 minimal medium containing casamino acids and glucose. Appropriate antibiotics were added to the following final concentrations: 100 μ g ml-1 ampicillin, 25 μ g ml-1 kanamycin, and 10 μ g ml-1 chloramphenicol.
DNA Manipulation and plasmid construction
Plasmid pET24D-YqiC (encoding full length YqiC protein) was generated by polymerase chain reaction (PCR) from Salmonella enterica serovar Typhimurium ATCC 14028 chromosome DNA using primers 5'-AACCATGGTTGACCCGAAAAAAATT-3' and 5'-TTCTCGAGCTCTTGTTGTGGATCGAC-3' and the product was cloned in Nco I and Xho I sites of pET24D vector (Novagen) in frame with the T7 promoter. The product included a six-histidine tag fused to the C-terminal end of the protein. To construct plasmid pBBR-yqiC, a 1210 bp fragment containing yqiC gene and flanking regions from S. Typhimurium was amplified by PCR using the primers 5'-GGCTTCAATGGTCACGGTAA-3' and 5'-GCAATATGGACGAGGAGCATC-3'. The resulting fragment was then cloned into the EcoRI site of the broad-host-range plasmid pBBR1MCS1 .
Expression and Purification of Recombinant Protein
pET24D plasmid encoding the sequence of yqiC was transformed in E. coli BL21 (lambda DE3). The cells were grown in LB at 37°C to an OD 600 of 0.5 and induced with 1 mM isopropyl β-D thiogalactoside (IPTG) for 4 h. Cells were harvested by centrifugation at 3000 × g for 20 min, resuspended in binding buffer (Qiagen), and disrupted by sonication with a probe tip sonicator. Total cell lysate was centrifuged at 14000 × g for 30 min to remove non-soluble protein, cell debris, and unbroken cells. Binding and elution from nickel nitrilotriacetic acid-agarose resin were carried out under native conditions according to the manufacturer's instructions (Qiagen). Eluted proteins were dialyzed against phosphate-buffered saline (pH 7.4). Proteins were assayed with a Coomassie blue-based staining solution.
Phospholipids were purchased from Avanti Polar Lipids (Birmingham, AL) and from Sigma. L-α-dipalmitoylphosphatidylcholine (DPPC) and L- α-dipalmitoylphosphatidic acid (DPPA) were cosolubilized in chloroform in different molar ratios, dried under N2, resuspended in buffer 50 mM Tris-HCl pH 8.0 or 50 mM sodium acetate pH 4.0 and sonicated to yield small unilamellar vesicles (SUV).
Purified YqiC was cross-linked with ethylene glycol bis (succinimidylsuccinate) (EGS) (Sigma) used at concentrations of 0.5, 1.0, and 5.0 mM. The reactions were carried out for 30 min at room temperature in phosphate-buffered saline and stopped by addition of 50 mM Tris-HCl, pH 8.0. Cross-linked products were analyzed by SDS-PAGE.
Determination of the Molecular Weight by Static Light Scattering
The average molecular weight (M w) of YqiC was determined on a Precision Detector PD2010 light scattering instrument tandemly connected to a high-performance liquid chromatography system and an LKB 2142 differential refractometer. The sample was loaded on a Superdex 75 column and eluted with PBS buffer. The 90° light scattering and refractive index signals of the eluting material were analyzed with Discovery32 software, supplied by Precision Detector. The 90° light scattering detector was calibrated using bovine serum albumin (66.5 kDa) as a standard.
Circular Dichroism Spectroscopy
The circular dichroism (CD) spectra of YqiC in the far-UV region (250-200 nm) were measured on a Jasco J-810 spectrophotometer using quartz cuvettes with a path length of 0.1 cm. The CD spectra were analyzed with K2D software http://www.ogic.ca/projects/k2d2/ to evaluate the secondary structure content.
Turbidity measurements were taken on a Multiskan Spectrum double-beam spectrophotometer (Thermo Electro Corp.) by using 1 cm matched silica cuvettes at 400 nm. The SUV concentration was 250 μ M. The lipid:protein ratio for the turbidity assays was kept at 50:1.
Vesicle Internal Content Mixing
Where F0 is the value of initial fluorescence of the vesicles, Ft is the value of fluorescence after incubation for t minutes with the protein, and Fmax is the value of fuorescence after addition of 0.2% of n-dodecyl maltoside.
Polyclonal anti-YqiC primary antibodies were obtained in mice immunized with purified YqiC. Immobilon-NC Transfer Membranes (Millipore) containing transferred proteins were blocked in 5% nonfat milk PBS for 1 h, and incubated with either a 1:200 dilution of polyclonal anti-YqiC or 1:200 anti-MBP mouse polyclonal antibodies. The secondary antibody used was goat anti-mouse IgG (Fc Specific) Peroxidase Conjugate (Sigma) at 1:1000 dilution. Positive signals were detected with Chemiluminiscent ECL Plus Western Blotting Detection System (Amersham Biosciences) on a Storm Image and Detection system (Molecular Dynamics).
Wild-type S. Typhimirium strain was grown in 80 mL LB medium to an OD600 of 1 and harvested by centrifugation at 4000 × g. The pellet was resuspended in 3 ml 20 mM Tris-HCl (pH 8.0) and 150 mM NaCl and mechanically lysed in a FastPrep instrument. Cell debris was removed by centrifugation for 30 min at 8000 × g. Subsequently, membranes were sedimented by ultracentrifugation for 1 h at 100,000 × g (4°C). The pellet was resuspended in a volume equivalent to that of the supernatant. Samples from the supernatant and pellet fraction were analyzed by immunoblotting.
Construction of yqiC S. Typhimurium mutant strain
Elimination of the yqiC gene was achieved by using Lambda Red-mediated recombination described previously . A lineal DNA fragment that contains the sequence for a chloramphenicol resistance cassette plus flanking regions of yqiC was constructed by PCR using the pair of primers 5'-CGCACTACAATAAGAGCTAACACTTACCAGTTCAGGGAAAGTGTAGGCTGGAGCTGCTTCG-3' and 5'-TGGATCGACTGGCGGAATGGCGGGCGCAGGTTTTACTTCTCATATGAATATCCTCCTTA-3'. 5 μg of this construction were introduced into strain LB5010 by electroporation. Chloramphenicol resistant colonies were then verified by PCR using a set of primers that hybridize within the insertion cassette and with an adjacent chromosomal region. Finally, isogenic strain was constructed by P22-mediated transduction of the mutant DNA into S. Typhimurium ATCC 14028. The substitution of the yqiC gene in this strain was verified by PCR and by the lack of expression of YqiC protein using western blot assay. The S. Typhimurium ΔyqiC::CAT mutant was named 14028 ΔyqiC::CAT.
To determine the 50% lethal dose (LD50) of the S. Typhimurium strains used, groups of seven 6-8 weeks old, female, BALB/c mice were infected intraperitoneally (i.p.) with serial 10-fold dilutions (from 1 × 101 to 1 × 105 CFU) of the wild type S. Typhimurium ATCC 14028 or 14028 ΔyqiC::CAT, and deaths were recorded for 28 days. For oral infections with S. Typhimurium ATCC 14028, 14028 ΔyqiC::CAT and 14028 ΔyqiC::CAT trans-complemented with pBBR-yqiC, mice were starved for food and water for 4 h. Following starvation, 105 CFU of each specific strain in 100 μl of phosphate-buffered saline (pH 7.4) were administered by oral gavage to each mouse. Survival of infected mice was recorded over 30 days. Inoculation doses were verified by serial dilution and plating into LB agar.
Cell invasion and intracellular replication
J774 murine macrophages and HeLa human epithelial cell lines were seeded at a density of 2 × 105 cells per well in 24-well culture plates. Stationary phase cultures of S. Typhimurium ATCC 14028, 14028 ΔyqiC::CAT and complemented strain 14028 ΔyqiC::CAT + pBBR-yqiC grown at 28°C overnight were added to the cells at a multiplicity of infection (MOI) of 10. Culture plates containing infected cells were centrifuged at 1000 rpm for 10 min and incubated at 37°C for 30 min to allow bacterial uptake and invasion. The extracellular bacteria were removed by washing thrice with PBS and incubating with 100 μg/ml gentamycin for 1 h. Thereafter, the cells were incubated with 25 μg/ml gentamycin for the rest of the experiment. After 1, 6 and 24 h, the cells were lysed with 1 mL of 0.1% Triton-X 100 per well and bacterial counts were determined by plating serial dilutions of the lysates on LB agar plates with appropriate antibiotic followed by incubation at 28°C.
This work was supported by grants from INTA (National project 472-AESA 2581) and Howard Hughes Medical Institute to Dr. Fernando Goldbaum (HHMI). The authors are researchers or are recipient of a fellowship from CONICET.
- Chuang CH, Su LH, Perera J, Carlos C, Tan BH, Kumarasinghe G, So T, Van PH, Chongthaleong A, Hsueh PR, Liu JW, Song JH, Chiu CH: Surveillance of antimicrobial resistance of Salmonella enterica serotype Typhi in seven Asian countries. Epidemiol Infect. 2009, 137: 266-269. 10.1017/S0950268808000745.PubMedView ArticleGoogle Scholar
- Hansen-Wester I, Hensel M: Salmonella pathogenicity islands encoding type III secretion systems. Microbes Infect. 2001, 3: 549-559. 10.1016/S1286-4579(01)01411-3.PubMedView ArticleGoogle Scholar
- Coburn B, Grassl GA, Finlay BB: Salmonella, the host and disease: a brief review, Immunol Cell Biol. 2007, 85: 112-118.Google Scholar
- McGhie EJ, Brawn LC, Hume PJ, Humphreys D, Koronakis V: Salmonella takes control: effector-driven manipulation of the host. Curr Opin Microbiol. 2009, 12: 117-124. 10.1016/j.mib.2008.12.001.PubMedPubMed CentralView ArticleGoogle Scholar
- Rodriguez-Morales O, Fernandez-Mora M, Hernandez-Lucas I, Vazquez A, Puente JL, Calva E: Salmonella enterica serovar Typhimurium ompS1 and ompS2 mutants are attenuated for virulence in mice. Infect Immun. 2006, 74: 1398-1402. 10.1128/IAI.74.2.1398-1402.2006.PubMedPubMed CentralView ArticleGoogle Scholar
- Chatfield SN, Dorman CJ, Hayward C, Dougan G: Role of ompR-dependent genes in Salmonella typhimurium virulence: mutants deficient in both ompC and ompF are attenuated in vivo. Infect Immun. 1991, 59: 449-452.PubMedPubMed CentralGoogle Scholar
- Su JH, Chung YC, Lee HC, Tseng IC, Chang MC: Ferrous iron-binding protein Omb of Salmonella enterica serovar Choleraesuis promotes resistance to hydrophobic antibiotics and contributes to its virulence. Microbiology. 2009, 155: 2365-2374. 10.1099/mic.0.026880-0.PubMedView ArticleGoogle Scholar
- Bjur E, Eriksson-Ygberg S, Aslund F, Rhen M: Thioredoxin 1 promotes intracellular replication and virulence of Salmonella enterica serovar Typhimurium. Infect Immun. 2006, 74: 5140-5151. 10.1128/IAI.00449-06.PubMedPubMed CentralView ArticleGoogle Scholar
- Carrica Mdel C, Craig PO, Alonso Sdel V, Goldbaum FA, Cravero SL: Brucella abortus MFP: a trimeric coiled-coil protein with membrane fusogenic activity. Biochemistry. 2008, 47: 8165-8175. 10.1021/bi800462y.PubMedView ArticleGoogle Scholar
- Bassford PJ, Silhavy TJ, Beckwith JR: Use of gene fusion to study secretion of maltose-binding protein into Escherichia coli periplasm. J Bacteriol. 1979, 139: 19-31.PubMedPubMed CentralGoogle Scholar
- Dutch RE, Jardetzky TS, Lamb RA: Virus membrane fusion proteins: biological machines that undergo a metamorphosis. Biosci Rep. 2000, 20: 597-612. 10.1023/A:1010467106305.PubMedView ArticleGoogle Scholar
- Parente RA, Nir S, Szoka FC: pH-dependent fusion of phosphatidylcholine small vesicles. Induction by a synthetic amphipathic peptide J Biol Chem. 1988, 263: 4724-4730.PubMedGoogle Scholar
- Celli J: Surviving inside a macrophage: the many ways of Brucella. Res Microbiol. 2006, 157: 93-98. 10.1016/j.resmic.2005.10.002.PubMedView ArticleGoogle Scholar
- Bakowski MA, Cirulis JT, Brown NF, Finlay BB, Brumell JH: SopD acts cooperatively with SopB during Salmonella enterica serovar Typhimurium invasion. Cell Microbiol. 2007, 9: 2839-2855. 10.1111/j.1462-5822.2007.01000.x.PubMedView ArticleGoogle Scholar
- Beuzon CR, Meresse S, Unsworth KE, Ruiz-Albert J, Garvis S, Waterman SR, Ryder TA, Boucrot E, Holden DW: Salmonella maintains the integrity of its intracellular vacuole through the action of SifA. Embo J. 2000, 19: 3235-3249. 10.1093/emboj/19.13.3235.PubMedPubMed CentralView ArticleGoogle Scholar
- Hayward RD, McGhie EJ, Koronakis V: Membrane fusion activity of purified SipB, a Salmonella surface protein essential for mammalian cell invasion. Mol Microbiol. 2000, 37: 727-739. 10.1046/j.1365-2958.2000.02027.x.PubMedView ArticleGoogle Scholar
- Collazo CM, Galan JE: The invasion-associated type III system of Salmonella typhimurium directs the translocation of Sip proteins into the host cell. Mol Microbiol. 1997, 24: 747-756. 10.1046/j.1365-2958.1997.3781740.x.PubMedView ArticleGoogle Scholar
- Mashburn-Warren LM, Whiteley M: Special delivery: vesicle trafficking in prokaryotes. Mol Microbiol. 2006, 61: 839-846. 10.1111/j.1365-2958.2006.05272.x.PubMedView ArticleGoogle Scholar
- Kesty NC, Mason KM, Reedy M, Miller SE, Kuehn MJ: Enterotoxigenic Escherichia coli vesicles target toxin delivery into mammalian cells. Embo J. 2004, 23: 4538-4549. 10.1038/sj.emboj.7600471.PubMedPubMed CentralView ArticleGoogle Scholar
- Mashburn LM, Whiteley M: Membrane vesicles traffic signals and facilitate group activities in a prokaryote. Nature. 2005, 437: 422-425. 10.1038/nature03925.PubMedView ArticleGoogle Scholar
- McBroom AJ, Kuehn MJ: Release of outer membrane vesicles by Gram-negative bacteria is a novel envelope stress response. Mol Microbiol. 2007, 63: 545-558.PubMedPubMed CentralView ArticleGoogle Scholar
- Fernandez-Moreira E, Helbig JH, Swanson MS: Membrane vesicles shed by Legionella pneumophila inhibit fusion of phagosomes with lysosomes. Infect Immun. 2006, 74: 3285-3295. 10.1128/IAI.01382-05.PubMedPubMed CentralView ArticleGoogle Scholar
- Schooling SR, Beveridge TJ: Membrane vesicles: an overlooked component of the matrices of biofilms. J Bacteriol. 2006, 188: 5945-5957. 10.1128/JB.00257-06.PubMedPubMed CentralView ArticleGoogle Scholar
- Deatherage BL, Lara JC, Bergsbaken T, Rassoulian Barrett SL, Lara S, Cookson BT: Biogenesis of bacterial membrane vesicles. Mol Microbiol. 2009, 72: 1395-1407. 10.1111/j.1365-2958.2009.06731.x.PubMedPubMed CentralView ArticleGoogle Scholar
- Kadurugamuwa JL, Beveridge TJ: Delivery of the non-membrane-permeative antibiotic gentamicin into mammalian cells by using Shigella flexneri membrane vesicles. Antimicrob Agents Chemother. 1998, 42: 1476-1483.PubMedPubMed CentralGoogle Scholar
- Hume PJ, McGhie EJ, Hayward RD, Koronakis V: The purified Shigella IpaB and Salmonella SipB translocators share biochemical properties and membrane topology. Mol Microbiol. 2003, 49: 425-439. 10.1046/j.1365-2958.2003.03559.x.PubMedView ArticleGoogle Scholar
- Kuehn MJ, Kesty NC: Bacterial outer membrane vesicles and the host-pathogen interaction. Genes Dev. 2005, 19: 2645-2655. 10.1101/gad.1299905.PubMedView ArticleGoogle Scholar
- Ono S, Goldberg MD, Olsson T, Esposito D, Hinton JC, Ladbury JE: H-NS is a part of a thermally controlled mechanism for bacterial gene regulation. Biochem J. 2005, 391: 203-213. 10.1042/BJ20050453.PubMedPubMed CentralView ArticleGoogle Scholar
- Mo E, Peters SE, Willers C, Maskell DJ, Charles IG: Single, double and triple mutants of Salmonella enterica serovar Typhimurium degP (htrA), degQ (hhoA) and degS (hhoB) have diverse phenotypes on exposure to elevated temperature and their growth in vivo is attenuated to different extents. Microb Pathog. 2006, 41: 174-182. 10.1016/j.micpath.2006.07.004.PubMedView ArticleGoogle Scholar
- Liu WT, Karavolos MH, Bulmer DM, Allaoui A, Hormaeche RD, Lee JJ, Khan CM: Role of the universal stress protein UspA of Salmonella in growth arrest, stress and virulence. Microb Pathog. 2007, 42: 2-10. 10.1016/j.micpath.2006.09.002.PubMedView ArticleGoogle Scholar
- Oliver SP, Gillespie BE, Ivey SJ, Lewis MJ, Johnson DL, Lamar KC, Moorehead H, Dowlen HH, Chester ST, Hallberg JW: Influence of prepartum pirlimycin hydrochloride or penicillin-novobiocin therapy on mastitis in heifers during early lactation. J Dairy Sci. 2004, 87: 1727-1731. 10.3168/jds.S0022-0302(04)73326-3.PubMedView ArticleGoogle Scholar
- Sciences CoL: Guide for the care and use of laboratory animals. 1996, Washington, D. CGoogle Scholar
- Kovach ME, Phillips RW, Elzer PH, Roop RM, Peterson KM: pBBR1MCS: a broad-host-range cloning vector. Biotechniques. 1994, 16: 800-802.PubMedGoogle Scholar
- Andrade MA, Chacon P, Merelo JJ, Moran F: Evaluation of secondary structure of proteins from UV circular dichroism spectra using an unsupervised learning neural network. Protein Eng. 1993, 6: 383-390. 10.1093/protein/6.4.383.PubMedView ArticleGoogle Scholar
- Duzgunes N, Wilschut J: Fusion assays monitoring intermixing of aqueous contents. Methods Enzymol. 1993, 220: 3-14.PubMedView ArticleGoogle Scholar
- Datsenko KA, Wanner BL: One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci USA. 2000, 97: 6640-6645. 10.1073/pnas.120163297.PubMedPubMed CentralView ArticleGoogle Scholar