SO2426 is a positive regulator of siderophore expression in Shewanella oneidensisMR-1
© Henne et al; licensee BioMed Central Ltd. 2011
Received: 23 August 2010
Accepted: 31 May 2011
Published: 31 May 2011
The Shewanella oneidensis MR-1 genome encodes a predicted orphan DNA-binding response regulator, SO2426. Previous studies with a SO2426-deficient MR-1 strain suggested a putative functional role for SO2426 in the regulation of iron acquisition genes, in particular, the siderophore (hydroxamate) biosynthesis operon so3030-3031-3032. To further investigate the functional role of SO2426 in iron homeostasis, we employed computational strategies to identify putative gene targets of SO2426 regulation and biochemical approaches to validate the participation of SO2426 in the control of siderophore biosynthesis in S. oneidensis MR-1.
In silico prediction analyses revealed a single 14-bp consensus motif consisting of two tandem conserved pentamers (5'-CAAAA-3') in the upstream regulatory regions of 46 genes, which were shown previously to be significantly down-regulated in a so2426 deletion mutant. These genes included so3030 and so3032, members of an annotated siderophore biosynthetic operon in MR-1. Electrophoretic mobility shift assays demonstrated that the SO2426 protein binds to its motif in the operator region of so3030. A "short" form of SO2426, beginning with a methionine at position 11 (M11) of the originally annotated coding sequence for SO2426, was also functional in binding to its consensus motif, confirming previous 5' RACE results that suggested that amino acid M11 is the actual translation start codon for SO2426. Alignment of SO2426 orthologs from all sequenced Shewanella spp. showed a high degree of sequence conservation beginning at M11, in addition to conservation of a putative aspartyl phosphorylation residue and the helix-turn-helix (HTH) DNA-binding domain. Finally, the so2426 deletion mutant was unable to synthesize siderophores at wild-type rates upon exposure to the iron chelator 2,2'-dipyridyl.
Collectively, these data support the functional characterization of SO2426 as a positive regulator of siderophore-mediated iron acquisition and provide the first insight into a coordinate program of multiple regulatory schemes controlling iron homeostasis in S. oneidensis MR-1.
Bacteria sense and respond to environmental stimuli primarily through signal transduction pathways, in which the canonical mechanism employs a sensor histidine kinase that interacts with a DNA-binding response regulator to activate or repress specific gene transcription [1, 2]. Some cellular processes have been shown to be controlled by orphan response regulators or one-component signalling systems, in which a cognate sensor kinase has not been elucidated . Orphan response regulators have been shown to be involved in the regulation of motility and chemotaxis , growth-phase-dependent responses [5, 6], virulence , iron transport  and oxidative stress responses [8, 9]. For instance, one well-characterized regulon that appears to be controlled by an orphan response regulator in S. oneidensis MR-1 is the ArcA regulon, which regulates the cellular response to aerobic and anaerobic respiratory conditions . The distinguishing feature of ArcA in comparison to the analogous system in Escherichia coli is that there does not seem to be a cognate sensor kinase, ArcB, in S. oneidensis , suggesting that S. oneidensis ArcA may be an orphan response regulator.
Our previous work suggested that a putative orphan response regulator, SO2426, in S. oneidensis MR-1 may be an integral member of a metal-responsive regulon governing the up-regulation of genes involved in iron uptake and homeostasis in response to metal stress. The ferric iron uptake regulator (Fur) is the predominant mechanism by which bacteria regulate iron homeostasis . Evidence suggests an additional iron responsive network regulated by SO2426 in S. oneidensis MR-1. Up-regulation of SO2426 at both the protein and transcript levels in response to iron and acid stress has been observed in a Δfur mutant strain of MR-1 [12–14]. Our previous studies investigating the transcriptomic and proteomic response of S. oneidensis to chromate challenge further revealed enhanced expression of so2426 under chromate stress [15, 16]. In a so2426 deletion mutant, genes involved in iron acquisition and homeostasis such as the so3030-3031-3032 operon, which encodes siderophore biosynthesis genes, were consistently down-regulated at high levels in the deletion mutant. Iron acquisition and storage systems are commonly up-regulated when bacteria are subjected to conditions of metal stress (e.g., chromate), and a link between iron transport and heavy metal sensitivity has been suggested [15, 17]. It is possible that sequestration of iron prevents redox cycling between ferrous iron and chromate, which can lead to reactive intermediates and oxidative stress [18, 19]. A consequence of this may be deficient intracellular iron concentrations that could inhibit growth. A cyclical response would ensue, resulting in up-regulation of iron uptake genes such as those involved in siderophore biosynthesis, which is similar to what has been demonstrated for S. oneidensis in response to chromate stress [15, 16, 20].
The aim of the present study was to examine the function of the uncharacterized SO2426 response regulator within the context of siderophore biosynthesis. We used a bioinformatics approach to map putative SO2426-binding domains and biochemical assays to demonstrate the binding of SO2426 to predicted recognition sites. Electrophoretic mobility shift assays showed that a recombinant SO2426 protein binds to a putative SO2426 motif that exists within the operator region of the so3030-3031-3032 operon. Siderophore detection assays further showed a diminished capacity of the Δso2426 mutant strain to produce siderophores, particularly in the presence of the iron chelator 2,2'-dipyridyl. Based on the identification of a Fur-binding motif upstream of the predicted SO2426-binding site within the operator region of the so3030-3031-3032 operon, we postulate that there are likely multiple levels of regulation operating in S. oneidensis MR-1 to precisely adjust intracellular iron levels in response to cellular needs. These intricate control mechanisms appear to involve Fur-mediated repression and derepression as well as SO2426-mediated activation of siderophore biosynthesis genes.
Results and Discussion
Conservation of SO2426 amino acid sequence among Shewanellae
In silicoprediction of the SO2426 recognition site
Putative SO2426 gene targets containing the predicted SO2426-binding site
Functional Category/Gene Product
bicyclomycin resistance protein
Central intermediary metabolism
adenosylhomocysteine nucleosidase, putative
acetyl-coenzyme A synthetase (acs)
conserved hypothetical protein
conserved hypothetical protein
glycerate kinase, putative
conserved hypothetical protein
conserved hypothetical protein
conserved hypothetical protein
Transport and binding proteins
TonB-dependent heme receptor
permease, GntP family
cation efflux family protein
siderophore biosynthesis protein (alcA)
siderophore biosynthesis protein, putative
ferric alcaligin siderophore receptor
sodium:alanine symporter family protein
ferric vibriobactin receptor (viuA)
TonB-dependent receptor, putative
DNA-binding response regulator
Several lines of evidence further support the role of so2426 in the regulation of iron acquisition in S. oneidensis MR-1. A recent study applying gene network reconstruction to MR-1 indicated that SO2426 clusters with iron acquisition genes in a distinct iron-responsive network system . Within this iron acquisition gene network were a number of members of the SO2426 regulon proposed here, namely, so1188, so1190, so3025, so3030-3031-3032, so3063, and so4743 . All of these genes, including so2426, were up-regulated under iron-depleted growth conditions compared to iron-replete conditions. Additionally, so3030 was up-regulated almost 14-fold in a fur mutant, while genes so3031-so3033 were up-regulated 4 to 11-fold . A separate transcriptomic study with a fur deletion mutant revealed that the gene with the greatest expression change in the fur mutant compared to the MR-1 wild-type strain was so2426, which showed a 30- and 26-fold increase in expression at the transcript level under aerobic and anaerobic growth conditions, respectively . In addition to the enhanced expression of so2426 in a fur mutant, this gene has been shown to be up-regulated under chromate [15, 41] and strontium  stress.
The presence of a putative Fur-binding sequence in the promoter region of so2426 suggests that expression of this response regulator may be subject to multiple levels of regulatory control. Identification of a Fur box immediately downstream of the -10 promoter element and up-regulation of so2426 expression in a fur deletion mutant are both consistent with repression of this gene by Fur under iron-sufficient conditions. Similarly, of those genes encoding transport and binding proteins, ftn, so1580, the so3030-3031-3032 operon, so4516, and so4743 are probable members of the Fur regulon based on their derepressed expression patterns in a S. oneidensis Δfur mutant and the presence of a putative Fur box in their respective upstream regions . Collectively, these observations suggest cross-regulation of iron-responsive and other metal-responsive gene networks in S. oneidensis MR-1.
SO2426 binds in region of predicted recognition site upstream of alcA
Given the potential overlap in the response of S. oneidensis to iron and other metals, we chose to focus on the S. oneidensis siderophore biosynthesis operon in testing the interaction of two recombinant versions of the SO2426 protein with its predicted binding motif. The direct involvement of so3030-3031-3032 in the production of hydroxamate-type siderophores was recently demonstrated with deletion mutants within this gene cluster . Induction of the so3030-3031-3032 operon in MR-1 cells under chromate challenge compared to unperturbed cells has been demonstrated using both transcriptomic and proteomic approaches [15, 16]. In further studies with a so2426 deletion mutant under chromate challenge, the so3030-3031-3032 operon was significantly down-regulated [21, 41]. These data, together with the predicted SO2426-binding motif upstream of so3030, suggest that SO2426 directly regulates siderophore production in strain MR-1 under conditions of chromate stress. We employed electrophoretic mobility shift assay (EMSA) to determine if the SO2426 protein was able to interact with the predicted binding sequence upstream of the so3030-3031-3032 operon.
Siderophore production is deficient in a Δso2426mutant strain
Earlier physiological evidence for the role of SO2426 in siderophore production was obtained using liquid CAS assays in which relative siderophore production levels for the Δso2426 mutant were compared to those for the wild-type MR-1 strain . These studies demonstrated that the deletion mutant was markedly deficient in siderophore synthesis compared to the wild-type strain in LB medium supplemented with chromate . LB medium constitutes a sufficient source of iron (~17 μM) . Additionally, under iron-replete conditions, in which 50 μm FeCl3 was added to the medium, there was no change in siderophore levels in the Δso2426 mutant. Conversely, siderophore production in the wild-type MR-1 strain returned to background levels in the presence of added iron .
The impaired ability of the Δso2426 mutant to produce siderophores during aerobic growth suggests that so2426 is a positive regulator of siderophore production in S. oneidensis MR-1. As noted earlier, several of the genes predicted to belong to the so2426 regulon also have Fur-binding motifs in their upstream regions. The likely molecular mechanism controlling iron homeostasis in S. oneidensis MR-1 involves Fur-mediated transcriptional repression, which includes down-regulation of so2426 expression under iron-replete conditions and derepression followed by SO2426-mediated transcriptional activation under iron-limited conditions. This may explain the residual siderophore production in the Δso2426 mutant. It is also possible that an as-yet uncharacterized secondary mechanism for siderophore production exists in strain MR-1.
SO2426 is annotated as a DNA-binding response regulator, but its specific function in S. oneidensis MR-1 was previously undefined. Using combined in silico motif prediction and in vitro binding assays along with physiological characterization, this report provides an important empirical step toward describing the SO2426 regulon. We initially identified a putative SO2426-binding consensus motif that consists of two conserved pentamers (5'-CAAAA-3') in tandem. Electrophoretic mobility shift assays demonstrated that recombinant SO2426 exhibits binding specificity with its predicted motif within the 5' regulatory region flanking a siderophore biosynthesis operon. A Δso2426 mutant was unable to synthesize CAS-reactive siderophores at wild-type rates under iron limitation. Collectively, these data support a function for SO2426 as a positive regulator of siderophore-mediated iron acquisition in S. oneidensis MR-1.
In addition to exhibiting iron-responsive expression, the so2426 gene has been previously shown to be up-regulated in response to chromate stress [15, 41]. The up-regulation of iron acquisition and iron storage systems in response to metal stress is not unique to S. oneidensis. In Arthrobacter sp. FB24, a number of proteins with putative functions in iron sequestration, such as Ferritin-Dps family proteins, as well as Reiske (2Fe-2S) domain proteins, showed increased abundance as a result of chromate stress . Copper has been shown to disrupt Fe-S clusters in important enzymes in E. coli . An E. coli strain defective in iron transport was also found to be more sensitive to chromium . Exposure to manganese in B. subtilis resulted in altered intracellular iron pools with subsequent expression of Fur-regulated genes . The reason for the up-regulation of iron-responsive genes is unclear. It has been speculated that metal ions such as chromate result in oxidative stress mediated through Fenton-type reactions with ferrous iron [18, 46–48]. Up-regulation of iron storage proteins may help alleviate metal-induced oxidative damage by binding excess Fe and preventing its interaction with other metal ions. It is also apparent that proteins with Fe-S prosthetic groups as part of their active centers are primary targets of metal-induced damage. These processes undoubtedly disrupt intracellular iron homeostasis, leading to the up-regulation of iron acquisition and sequestration systems. The evidence provided here and in our previous work strongly points to an integral role of SO2426 in such iron control systems.
Bacterial strains, plasmids, and culture conditions
Bacterial strains and plasmids used in this study
Shewanella oneidensis MR-1
ATCC 7005500 Lab stock
Deletion of so2426 locus
E. coli TOP10
Cloning and expression strain
E. coli ER2508
Major proteinase-deficient strain
New England Biolabs
Expresses full-length SO2426 protein
Expresses truncated SO2426 protein
E. coli (pTOPO)
so2426sh cloned in frame with N-terminal polyhistidine
so2426 cloned in frame with N-terminal polyhistidine
SO2426 weight matrix development and identification of a putative SO2426 recognition site
MEME , MotifSampler , and Gibbs Recursive Sampler  were used to predict promoter recognition sequences potentially bound by SO2426. To facilitate motif searching, the time-series microarray expression profiles of the Δso2426 relative to the parental strain were clustered using Hierarchical Clustering Explorer (HCE) . During the clustering process, only genes with an expression value of at least ≥ 2-fold or ≤ 0.5-fold in one or more of 6 expression profiling time points were included in the analyses. As a result, a dataset of 841 genes was clustered based on the average linkage using Euclidean distance . We extracted a sub-cluster comprising 46 similarly down-regulated genes throughout the 6 time points, and this dataset was used as the input data for putative SO2426 binding-site prediction. The consensus SO2426-binding sequence was predicted with MEME using the following parameters: (i) the motif width ranged from 6 to 50; (ii) the total number of sites in the training set where a single motif occurred was 3; and (iii) the sequence had 0 or 1 binding site. MAST  was used to scan the sequence database with the predicted MEME-derived motif. The Gibbs Recursive Sampler program was performed as described previously . MotifSampler  was employed to confirm the consensus motif predicted using MEME and Gibbs Recursive Sampler. A sequence logo  was generated to graphically represent the sequence conservation of the predicted SO2426 recognition site.
Sequence analysis of SO2426 orthologs
ClustalW  was used to perform a multiple sequence alignment of Shewanella SO2426 orthologs. Conserved signature residues in the receiver domain of response regulators were annotated based on reference . The phylogenetic tree was constructed based on protein sequences using maximum parsimony method implemented in PAUP* version 4.0 Beta . The bootstrap values were generated using maximum parsimony. The GenBank accession numbers are as follows: YP_734035.1, Shewanella sp. MR-4; YP_738119.1Shewanella sp. MR-7; YP_750834.1, Shewanella frigidimarina NCIMB 400; YP_869596.1, Shewanella sp. ANA-3; YP_927593.1, Shewanella amazonensis SB2B; YP_963447.1, Shewanella sp. W3-18-1; ZP_01705802.1, Shewanella putrefaciens 200; YP_001050420.1, Shewanella baltica OS155; YP_001094061.1, Shewanella loihica PV-4; YP_001366502.1, Shewanella baltica OS185; YP_001474053.1, Shewanella sediminis HAW-EB3; YP_001502091.1, Shewanella pealeana ATCC 700345; YP_001554844.1, Shewanella baltica OS195; ZP_02156174.1, Shewanella benthica KT99; YP_001674438.1, Shewanella halifaxensis HAW-EB4; YP_001760668.1, Shewanella woodyi ATCC 51908; YP_002311920.1, Shewanella piezotolerans WP3; YP_002357973.1, Shewanella baltica OS223; NP_718016.1, Shewanella oneidensis MR-1; and YP_562912.1, Shewanella denitrificans OS217.
The chrome azurol-S (CAS)-based assay for detection of siderophore production during cellular growth in liquid was performed as described elsewhere [21, 55] with slight modifications in culture conditions. Overnight LB cultures of the Δso2426 strain and the wild-type MR-1 strain were used to inoculate fresh LB broth and allowed to grow to mid-logarithmic phase (OD600 ~ 0.6). The mid-log-phase cultures were amended with 50 μM FeCl3, 80 μM 2,2'-dipyridyl, or 0.3 mM K2CrO4. A control consisting of LB without amendment was prepared for each strain. The cultures were allowed to incubate for 24 h at 30°C with shaking. Aliquots were taken for CAS assay analysis at 0, 2, 4, 6, 8, and 24 h post amendment. Cell-free supernatants were mixed 1:1 with the CAS assay solution and equilibrated at room temperature for 2 h prior to reading the absorbance at 630 nm. The relative production of CAS-reactive siderophores was calculated as described  and reported as the average of three independent experiments.
Expression and partial purification of recombinant SO2426 protein
Bacterial expression vectors were constructed by cloning the full-length SO2426 gene and a shortened form (SO2426sh) in frame with the N-terminal His-tag of pTrcHis (Invitrogen, Carlsbad, CA). Plasmids were transformed into E. coli TOP10 (Invitrogen, Carlsbad, CA) or E. coli ER2508 (New England Biolabs, Ipswich, MA) host cells. Transformants were selected on LB-ampicillin agar plates. Positive clones were verified by sequence analysis at the Purdue Genomics Core Facility.
Cells carrying the expression vectors were grown at 37°C in 100 ml of LB with 50 μg/ml ampicillin until a cell density of ~0.6 was attained. IPTG was added to a concentration of 1 mM, and the cultures were incubated for an additional 3 hours to induce expression of recombinant SO2426 proteins. Cells were harvested by centrifugation and washed in 1X TBS. Cell lysates were prepared by sonicating cell pellets in Guanidium Lysis Buffer, pH 7.8 (Invitrogen, Carlsbad, CA) containing 1X Complete-Mini Protease Inhibitor Cocktail (Roche Applied Science, Indianapolis, IN). The lysates were centrifuged at 6,000 RPM for 10 min to remove cell debris. His-tagged proteins were recovered from cell lysates using the ProBond Purification System (Invitrogen, Carlsbad, CA) under hybrid conditions as specified by the manufacturer's protocol. A total of eight 1 to 2-ml elution fractions were collected for each protein extract.
Verification of SO2426 recombinant protein
Expression of His-tagged SO2426 and SO2426sh proteins in the elution fractions was verified by Western blot analysis using the Western Breeze Chromogenic Western Blot Immunodetection Kit (Invitrogen, Carlsbad, CA). His-tagged proteins were probed with an anti-HisG antibody (Invitrogen, Carlsbad, CA) with secondary detection using anti-mouse IgG-alkaline phosphatase antibody provided in the Western Breeze kit. Positive elution fractions were pooled and concentrated with YM-3 Centricon Centrifugal Filter Devices (Millipore, Billerica, MA). Concentrated fractions were dialyzed overnight at 4°C against TED buffer [20 mM Tris-Cl (pH 7.0), 150 mM NaCl, 0.1 mM EDTA, and 0.1 mM DTT] using mini dialysis tubes with a molecular weight cutoff of 8 kDa. Protein concentration was determined using a Nanodrop ND-1000 Spectrophotometer (Rockland, DE).
Electrophoretic Mobility Shift Assay (EMSA)
Oligonucleotide primers used in this study
DNA sequence (5' → 3')
Reference or source
EMSA was performed in 20-μl reaction volumes containing 0.5X EMSA buffer [5 mM Tris-Cl (pH 8.0), 75 mM KCl, 0.05 mM DTT, 0.05 mM EDTA, 6% glycerol], 5 mM MgCl2, 20 mM Acetyl-PO4, 0.2 μg/μl poly(dI:dC), 0.2 μg/μl BSA, and 95 ng DIG-labeled DNA probe. Protein was added in concentrations ranging from 0.6 to 3.0 μg in increments of 0.6 μg. Reactions were incubated at 16°C for 30 min. NP-40 was added to each reaction mixture at a concentration of 0.1% prior to separation on a pre-run 5% polyacrylamide gel. Gels were stained with SYBR green and then transferred onto Hybond N+ (Amersham Biosciences, Piscataway, NJ). Probing and detection of DIG-labeled DNA was performed with the DIG Nucleic Acid Detection Kit according to the manufacturer's protocol for colorimetric detection.
We thank Andrea McCarthy for assistance with the siderophore production assays and Mauricio Barajas for assistance with recombinant protein expression. This research was supported in part by the Office of Science (BER), U.S. Department of Energy, Grant No. DE-FG02-06ER64163, to DKT.
- Raivio TL, Silhavy TJ: Periplasmic stress and ECF sigma factors. Annu Rev Microbiol. 2001, 55: 591-624. 10.1146/annurev.micro.55.1.591.PubMedView ArticleGoogle Scholar
- West AH, Stock AM: Histidine kinases and response regulator proteins in two-component signaling systems. Trends Biochem Sci. 2001, 26: 369-376. 10.1016/S0968-0004(01)01852-7.PubMedView ArticleGoogle Scholar
- Ulrich LE, Koonin EV, Zhulin IB: One-component systems dominate signal transduction in prokaryotes. Trends Microbiol. 2005, 13: 52-56. 10.1016/j.tim.2004.12.006.PubMedPubMed CentralView ArticleGoogle Scholar
- Gueriri I, Cyncynatus C, Dubrac S, Arana AT, Dussurget O, Msadek T: The DegU orphan response regulator of Listeria monocytogenes autorepresses its own synthesis and is required for bacterial motility, virulence and biofilm formation. Microbiology. 2008, 154: 2251-2264. 10.1099/mic.0.2008/017590-0.PubMedView ArticleGoogle Scholar
- Delany I, Spohn G, Rappuoli R, Scarlato V: Growth phase-dependent regulation of target gene promoters for binding of the essential orphan response regulator HP1043 of Helicobacter pylori. J Bacteriol. 2002, 184: 4800-4810. 10.1128/JB.184.17.4800-4810.2002.PubMedPubMed CentralView ArticleGoogle Scholar
- Hong E, Lee HM, Ko H, Kim DU, Jeon BY, Jung J, Shin J, Lee SA, Kim Y, Jeon YH, et al: Structure of an atypical orphan response regulator protein supports a new phosphorylation-independent regulatory mechanism. J Biol Chem. 2007, 282: 20667-20675. 10.1074/jbc.M609104200.PubMedView ArticleGoogle Scholar
- Pan X, Ge J, Li M, Wu B, Wang C, Wang J, Feng Y, Yin Z, Zheng F, Cheng G, et al: The orphan response regulator CovR: a globally negative modulator of virulence in Streptococcus suis serotype 2. J Bacteriol. 2009, 191: 2601-2612. 10.1128/JB.01309-08.PubMedPubMed CentralView ArticleGoogle Scholar
- Ulijasz AT, Andes DR, Glasner JD, Weisblum B: Regulation of iron transport in Streptococcus pneumoniae by RitR, an orphan response regulator. J Bacteriol. 2004, 186: 8123-8136. 10.1128/JB.186.23.8123-8136.2004.PubMedPubMed CentralView ArticleGoogle Scholar
- Echave P, Tamarit J, Cabiscol E, Ros J: Novel antioxidant role of alcohol dehydrogenase E from Escherichia coli. J Biol Chem. 2003, 278: 30193-30198. 10.1074/jbc.M304351200.PubMedView ArticleGoogle Scholar
- Gao H, Wang X, Yang ZK, Palzkill T, Zhou J: Probing regulon of ArcA in Shewanella oneidensis MR-1 by integrated genomic analyses. BMC Genomics. 2008, 9: 42-10.1186/1471-2164-9-42.PubMedPubMed CentralView ArticleGoogle Scholar
- Andrews SC, Robinson AK, Rodriguez-Quinones F: Bacterial iron homeostasis. FEMS Microbiol Rev. 2003, 27: 215-237. 10.1016/S0168-6445(03)00055-X.PubMedView ArticleGoogle Scholar
- Wan XF, Verberkmoes NC, McCue LA, Stanek D, Connelly H, Hauser LJ, Wu L, Liu X, Yan T, Leaphart A, et al: Transcriptomic and proteomic characterization of the Fur modulon in the metal-reducing bacterium Shewanella oneidensis. J Bacteriol. 2004, 186: 8385-8400. 10.1128/JB.186.24.8385-8400.2004.PubMedPubMed CentralView ArticleGoogle Scholar
- Yang Y, Harris DP, Luo F, Wu L, Parsons AB, Palumbo AV, Zhou J: Characterization of the Shewanella oneidensis Fur gene: roles in iron and acid tolerance response. BMC Genomics. 2008, 9 (Suppl 1): S11-10.1186/1471-2164-9-S1-S11.View ArticleGoogle Scholar
- Yang Y, Harris DP, Luo F, Xiong W, Joachimiak M, Wu L, Dehal P, Jacobsen J, Yang Z, Palumbo AV, et al: Snapshot of iron response in Shewanella oneidensis by gene network reconstruction. BMC Genomics. 2009, 10: 131-10.1186/1471-2164-10-131.PubMedPubMed CentralView ArticleGoogle Scholar
- Brown SD, Thompson MR, Verberkmoes NC, Chourey K, Shah M, Zhou J, Hettich RL, Thompson DK: Molecular dynamics of the Shewanella oneidensis response to chromate stress. Mol Cell Proteomics. 2006, 5: 1054-1071. 10.1074/mcp.M500394-MCP200.PubMedView ArticleGoogle Scholar
- Thompson MR, VerBerkmoes NC, Chourey K, Shah M, Thompson DK, Hettich RL: Dosage-dependent proteome response of Shewanella oneidensis MR-1 to acute chromate challenge. J Proteome Res. 2007, 6: 1745-1757. 10.1021/pr060502x.PubMedView ArticleGoogle Scholar
- Henne KL, Turse JE, Nicora CD, Lipton MS, Tollaksen SL, Lindberg C, Babnigg G, Giometti CS, Nakatsu CH, Thompson DK, Konopka AE: Global proteomic analysis of the chromate response in Arthrobacter sp. strain FB24. J Proteome Res. 2009, 8: 1704-1716. 10.1021/pr800705f.PubMedView ArticleGoogle Scholar
- Bagchi D, Stohs SJ, Downs BW, Bagchi M, Preuss HG: Cytotoxicity and oxidative mechanisms of different forms of chromium. Toxicology. 2002, 180: 5-22. 10.1016/S0300-483X(02)00378-5.PubMedView ArticleGoogle Scholar
- Wang CC, Newton A: Iron transport in Escherichia coli: relationship between chromium sensitivity and high iron requirement in mutants of Escherichia coli. J Bacteriol. 1969, 98: 1135-1141.PubMedPubMed CentralGoogle Scholar
- Chourey K, Thompson MR, Morrell-Falvey J, Verberkmoes NC, Brown SD, Shah M, Zhou J, Doktycz M, Hettich RL, Thompson DK: Global molecular and morphological effects of 24-hour chromium(VI) exposure on Shewanella oneidensis MR-1. Appl Environ Microbiol. 2006, 72: 6331-6344. 10.1128/AEM.00813-06.PubMedPubMed CentralView ArticleGoogle Scholar
- Chourey K, Wei W, Wan XF, Thompson DK: Transcriptome analysis reveals response regulator SO2426-mediated gene expression in Shewanella oneidensis MR-1 under chromate challenge. BMC Genomics. 2008, 9: 395-10.1186/1471-2164-9-395.PubMedPubMed CentralView ArticleGoogle Scholar
- Martinez-Hackert E, Stock AM: Structural relationships in the OmpR family of winged-helix transcription factors. J Mol Biol. 1997, 269: 301-312. 10.1006/jmbi.1997.1065.PubMedView ArticleGoogle Scholar
- Konstantinidis KT, Serres MH, Romine MF, Rodrigues JL, Auchtung J, McCue LA, Lipton MS, Obraztsova A, Giometti CS, Nealson KH, et al: Comparative systems biology across an evolutionary gradient within the Shewanella genus. Proc Natl Acad Sci USA. 2009, 106: 15909-15914. 10.1073/pnas.0902000106.PubMedPubMed CentralView ArticleGoogle Scholar
- Hau HH, Gralnick JA: Ecology and biotechnology of the genus Shewanella. Annu Rev Microbiol. 2007, 61: 237-258. 10.1146/annurev.micro.61.080706.093257.PubMedView ArticleGoogle Scholar
- Saltikov CW, Cifuentes A, Venkateswaran K, Newman DK: The ars detoxification system is advantageous but not required for As(V) respiration by the genetically tractable Shewanella species strain ANA-3. Appl Environ Microbiol. 2003, 69: 2800-2809. 10.1128/AEM.69.5.2800-2809.2003.PubMedPubMed CentralView ArticleGoogle Scholar
- Aguilar-Barajas E, Paluscio E, Cervantes C, Rensing C: Expression of chromate resistance genes from Shewanella sp. strain ANA-3 in Escherichia coli. FEMS Microbiol Lett. 2008, 285: 97-100. 10.1111/j.1574-6968.2008.01220.x.PubMedView ArticleGoogle Scholar
- Bencheikh-Latmani R, Obraztsova A, Mackey MR, Ellisman MH, Tebo BM: Toxicity of Cr(lll) to Shewanella sp. strain MR-4 during Cr(VI) reduction. Environ Sci Technol. 2007, 41: 214-220. 10.1021/es0622655.PubMedView ArticleGoogle Scholar
- Karpinets TV, Obraztsova AY, Wang Y, Schmoyer DD, Kora GH, Park BH, Serres MH, Romine MF, Land ML, Kothe TB, et al: Conserved synteny at the protein family level reveals genes underlying Shewanella species' cold tolerance and predicts their novel phenotypes. Funct Integr Genomics. 10: 97-110.
- Fredrickson JK, Romine MF, Beliaev AS, Auchtung JM, Driscoll ME, Gardner TS, Nealson KH, Osterman AL, Pinchuk G, Reed JL, et al: Towards environmental systems biology of Shewanella. Nat Rev Microbiol. 2008, 6: 592-603. 10.1038/nrmicro1947.PubMedView ArticleGoogle Scholar
- Bailey TL, Elkan C: Fitting a mixture model by expectation maximization to discover motifs in biopolymers. Proc Int Conf Intell Syst Mol Biol. 1994, 2: 28-36.PubMedGoogle Scholar
- Thijs G, Marchal K, Lescot M, Rombauts S, De Moor B, Rouze P, Moreau Y: A Gibbs sampling method to detect overrepresented motifs in the upstream regions of coexpressed genes. J Comput Biol. 2002, 9: 447-464. 10.1089/10665270252935566.PubMedView ArticleGoogle Scholar
- Thompson W, Rouchka EC, Lawrence CE: Gibbs Recursive Sampler: finding transcription factor binding sites. Nucleic Acids Res. 2003, 31: 3580-3585. 10.1093/nar/gkg608.PubMedPubMed CentralView ArticleGoogle Scholar
- De Wulf P, McGuire AM, Liu X, Lin EC: Genome-wide profiling of promoter recognition by the two-component response regulator CpxR-P in Escherichia coli. J Biol Chem. 2002, 277: 26652-26661. 10.1074/jbc.M203487200.PubMedView ArticleGoogle Scholar
- Pogliano J, Lynch AS, Belin D, Lin EC, Beckwith J: Regulation of Escherichia coli cell envelope proteins involved in protein folding and degradation by the Cpx two-component system. Genes Dev. 1997, 11: 1169-1182. 10.1101/gad.11.9.1169.PubMedView ArticleGoogle Scholar
- Danese PN, Snyder WB, Cosma CL, Davis LJ, Silhavy TJ: The Cpx two-component signal transduction pathway of Escherichia coli regulates transcription of the gene specifying the stress-inducible periplasmic protease, DegP. Genes Dev. 1995, 9: 387-398. 10.1101/gad.9.4.387.PubMedView ArticleGoogle Scholar
- Ruiz N, Silhavy TJ: Sensing external stress: watchdogs of the Escherichia coli cell envelope. Curr Opin Microbiol. 2005, 8: 122-126. 10.1016/j.mib.2005.02.013.PubMedView ArticleGoogle Scholar
- Batchelor E, Walthers D, Kenney LJ, Goulian M: The Escherichia coli CpxA-CpxR envelope stress response system regulates expression of the porins ompF and ompC. J Bacteriol. 2005, 187: 5723-5731. 10.1128/JB.187.16.5723-5731.2005.PubMedPubMed CentralView ArticleGoogle Scholar
- De Wulf P, Kwon O, Lin EC: The CpxRA signal transduction system of Escherichia coli: growth-related autoactivation and control of unanticipated target operons. J Bacteriol. 1999, 181: 6772-6778.PubMedPubMed CentralGoogle Scholar
- Danese PN, Silhavy TJ: CpxP, a stress-combative member of the Cpx regulon. J Bacteriol. 1998, 180: 831-839.PubMedPubMed CentralGoogle Scholar
- Yamamoto K, Ishihama A: Transcriptional response of Escherichia coli to external copper. Mol Microbiol. 2005, 56: 215-227. 10.1111/j.1365-2958.2005.04532.x.PubMedView ArticleGoogle Scholar
- Chourey K, Thompson MR, Shah M, Zhang B, Verberkmoes NC, Thompson DK, Hettich RL: Comparative temporal proteomics of a response regulator (SO2426)-deficient strain and wild-type Shewanella oneidensis MR-1 during chromate transformation. J Proteome Res. 2009, 8: 59-71. 10.1021/pr800776d.PubMedView ArticleGoogle Scholar
- Brown SD, Martin M, Deshpande S, Seal S, Huang K, Alm E, Yang Y, Wu L, Yan T, Liu X, et al: Cellular response of Shewanella oneidensis to strontium stress. Appl Environ Microbiol. 2006, 72: 890-900. 10.1128/AEM.72.1.890-900.2006.PubMedPubMed CentralView ArticleGoogle Scholar
- Fennessey CM, Jones ME, Taillefert M, DiChristina TJ: Siderophores are not involved in Fe(III) solubilization during anaerobic Fe(III) respiration by Shewanella oneidensis MR-1. Appl Environ Microbiol. 2010, 76: 2425-2432. 10.1128/AEM.03066-09.PubMedPubMed CentralView ArticleGoogle Scholar
- Macomber L, Imlay JA: The iron-sulfur clusters of dehydratases are primary intracellular targets of copper toxicity. Proc Natl Acad Sci USA. 2009, 106: 8344-8349. 10.1073/pnas.0812808106.PubMedPubMed CentralView ArticleGoogle Scholar
- Guedon E, Moore CM, Que Q, Wang T, Ye RW, Helmann JD: The global transcriptional response of Bacillus subtilis to manganese involves the MntR, Fur, TnrA and sigmaB regulons. Mol Microbiol. 2003, 49: 1477-1491. 10.1046/j.1365-2958.2003.03648.x.PubMedView ArticleGoogle Scholar
- Myers CR, Myers JM: Iron stimulates the rate of reduction of hexavalent chromium by human microsomes. Carcinogenesis. 1998, 19: 1029-1038. 10.1093/carcin/19.6.1029.PubMedView ArticleGoogle Scholar
- Myers CR, Myers JM, Carstens BP, Antholine WE: Reduction Of Chromium(VI) To Chromium(V) By Human Microsomal Enzymes: Effects Of Iron and Quinones. Toxic Substance Mechanisms. 2000, 19: 25-51. 10.1080/10769180051125734.View ArticleGoogle Scholar
- Luo H, Lu Y, Shi X, Mao Y, Dalal NS: Chromium (IV)-mediated fenton-like reaction causes DNA damage: implication to genotoxicity of chromate. Ann Clin Lab Sci. 1996, 26: 185-191.PubMedGoogle Scholar
- Seo J, Gordish-Dressman H, Hoffman EP: An interactive power analysis tool for microarray hypothesis testing and generation. Bioinformatics. 2006, 22: 808-814. 10.1093/bioinformatics/btk052.PubMedView ArticleGoogle Scholar
- Bailey TL, Gribskov M: Combining evidence using p-values: application to sequence homology searches. Bioinformatics. 1998, 14: 48-54. 10.1093/bioinformatics/14.1.48.PubMedView ArticleGoogle Scholar
- Schneider TD, Stephens RM: Sequence logos: a new way to display consensus sequences. Nucleic Acids Res. 1990, 18: 6097-6100. 10.1093/nar/18.20.6097.PubMedPubMed CentralView ArticleGoogle Scholar
- Thompson JD, Higgins DG, Gibson TJ: CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 1994, 22: 4673-4680. 10.1093/nar/22.22.4673.PubMedPubMed CentralView ArticleGoogle Scholar
- Stock AM, Robinson VL, Goudreau PN: Two-component signal transduction. Annu Rev Biochem. 2000, 69: 183-215. 10.1146/annurev.biochem.69.1.183.PubMedView ArticleGoogle Scholar
- Swofford DL: PAUP*: Phylogenic analysis using Parsimony. 1998, Sinauer, Sunderland, MassachusettsGoogle Scholar
- Schwyn B, Neilands JB: Universal chemical assay for the detection and determination of siderophores. Anal Biochem. 1987, 160: 47-56. 10.1016/0003-2697(87)90612-9.PubMedView ArticleGoogle Scholar
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