Correction: Surveillance of pyrazinamide susceptibility among multidrug-resistant Mycobacterium tuberculosis isolates from Siriraj Hospital, Thailand

  • Jirarut Jonmalung1,

    Affiliated with

    • Therdsak Prammananan2, 4,

      Affiliated with

      • Manoon Leechawengwongs3, 4 and

        Affiliated with

        • Angkana Chaiprasert1, 4Email author

          Affiliated with

          BMC Microbiology201010:278

          DOI: 10.1186/1471-2180-10-278

          Received: 28 October 2010

          Accepted: 9 November 2010

          Published: 9 November 2010

          Correction

          After galley proof of the manuscript, we found three mistakes of the nucleotide positions (G222C, G364A and C520T) and codon numbers (Gly74Arg, Gly122Ser and Thr174Ile) that have to be corrected but it was unable to make any change because the publication of this work is on going [1]. After revision, Table two (Table 1 in this manuscript) and some information in the discussion part were changed. There were only 5 novel mutation types found in this study, consisting of 2 nucleotide substitutions (Leu27Pro and Thr174Ile), 2 nucleotide insertions (G insertion between nucleotide 411 and 412 and GG insertion between nucleotide 520 and 521), and 1 nonsense mutation at Glu127.
          Table 1

          Results of pncA gene sequencing of 150 M. tuberculosis clinical isolates.

             

          pncA mutation

          M. tuberculosis strains (no. of isolates)

          MGIT 960

          PZase assay

          Nucleotide change

          Amino acid change

          Susceptible (46)

          S

          +

          wild-type

          no

          Susceptible (1)

          S

          +

          T92G

          Ile31Ser

          Susceptible (2)

          R

          +

          wild-type

          wild-type

          Susceptible (1)

          R

          +

          T92C

          Ile31Thr

          MDR-TB (42)

          S

          +

          wild-type

          wild-type

          MDR-TB (9)

          S

          +

          T92C

          Ile31Thr

          MDR-TB (34)

          R

          -

          A(-11)G (1)

          no

             

          A(-11)C (1)

          no

             

          T56G (1)

          Leu19Arg

             

          T80C (1)

          Leu27Pro

             

          T92G (2)

          Ile31Ser

             

          T104C (1)

          Leu35Pro

             

          T134C (1)

          Val45Ala

             

          G136T (1)

          Ala46Ser

             

          T199C (1)

          Ser67Pro

             

          C211G (8)

          His71Asp

             

          G215A (1)

          Cys72Tyr

             

          G289A (3)

          Gly97Ser

             

          C312G (2)

          Ser104Arg

             

          G322C (1)

          Gly108Arg

             

          G373T (1)

          Val125Phe

             

          G379T (1)

          Glu 127 Stop

             

          G394A (1)

          Gly132Ser

             

          G insertion b/w 411-412 (1)

           
             

          T416G (1)

          Val 139 Gly

             

          C425T (1)

          Thr 142 Met

             

          G436A (1)

          Ala 146 Thr

             

          GG insertion b/w 520-521 (1)

           
             

          C530T (1)

          Thr 177 Ile

          MDR-TB (11)

          R

          +

          wild-type

          no

          MDR-TB (4)

          R

          +

          T92C (3)

          Ile31Thr

             

          T92G (1)

          Ile31Ser

          We regret any inconvenience that the mistake might have caused. We wish to thank Dr. Claudio Köser, Department of Genetics, University of Cambridge, for bringing this matter to our attention.

          Authors’ Affiliations

          (1)
          Department of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University
          (2)
          National Center for Genetic Engineering and Biotechnology, National Science and Technology, Development Agency, Ministry of Science and Technology
          (3)
          Vichaiyut Hospital
          (4)
          Drug-Resistant Tuberculosis Research Fund, Siriraj Foundation

          References

          1. Jonmalung J, Prammananan T, Leechawengwongs M, Chaiprasert A: Surveillance of pyrazinamide susceptibility among multidrug-resistant Mycobacterium tuberculosis isolates from Siriraj Hospital, Thailand. BMC Microbiology 2010, 10:223.PubMedView Article

          Copyright

          © Jonmalung et al. 2010

          This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://​creativecommons.​org/​licenses/​by/​2.​0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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