The T box regulatory element controlling expression of the class I lysyl-tRNA synthetase of Bacillus cereus strain 14579 is functional and can be partially induced by reduced charging of asparaginyl-tRNAAsn
© Foy et al; licensee BioMed Central Ltd. 2010
Received: 5 May 2010
Accepted: 22 July 2010
Published: 22 July 2010
Lysyl-tRNA synthetase (LysRS) is unique within the aminoacyl-tRNA synthetase family in that both class I (LysRS1) and class II (LysRS2) enzymes exist. LysRS1 enzymes are found in Archaebacteria and some eubacteria while all other organisms have LysRS2 enzymes. All sequenced strains of Bacillus cereus (except AH820) and Bacillus thuringiensis however encode both a class I and a class II LysRS. The lysK gene (encoding LysRS1) of B. cereus strain 14579 has an associated T box element, the first reported instance of potential T box control of LysRS expression.
A global study of 891 completely sequenced bacterial genomes identified T box elements associated with control of LysRS expression in only four bacterial species: B. cereus, B. thuringiensis, Symbiobacterium thermophilum and Clostridium beijerinckii. Here we investigate the T box element found in the regulatory region of the lysK gene in B. cereus strain 14579. We show that this T box element is functional, responding in a canonical manner to an increased level of uncharged tRNALys but, unusually, also responding to an increased level of uncharged tRNAAsn. We also show that B. subtilis strains with T box regulated expression of the endogenous lysS or the heterologous lysK genes are viable.
The T box element controlling lysK (encoding LysRS1) expression in B. cereus strain 14579 is functional, but unusually responds to depletion of charged tRNALys and tRNAAsn. This may have the advantage of making LysRS1 expression responsive to a wider range of nutritional stresses. The viability of B. subtilis strains with a single LysRS1 or LysRS2, whose expression is controlled by this T box element, makes the rarity of the occurrence of such control of LysRS expression puzzling.
The aminoacyl tRNA synthetase (AARS) family of enzymes function to attach amino acids to their cognate tRNAs [1–3]. Each enzyme specifically charges a tRNA with its cognate amino acid in an energy requiring reaction that is executed with very high fidelity. However, despite all AARSs carrying out essentially the same reaction, the AARS family is subdivided into class I and class II enzymes that are structurally distinct and unrelated phylogenetically [for reviews see [3, 4]]. This division of AARS into class I and class II enzymes is universal with each AARS being a member of one or other enzyme class in all living organisms. The lysyl-tRNA synthetase (LysRS) is an exception in that both class I (LysRS1) and class II (LysRS2) variants exist [5, 6]. LysRS1 enzymes are found in Archaebacteria and in some eubacteria (eg. Borrelia and Treponema species) while LysRS2 enzymes are found in most eubacteria and all eukaryotes. Interestingly some bacteria have both class I LysRS1 and class II LysRS2 enzymes. For example, in Methanosarcina barkeri the class I and class II LysRS enzymes function as a complex to charge tRNAPyl with the rare pyrolysine amino acid while in B. cereus strain 14579 both enzymes can function together to aminoacylate a small tRNA-like molecule (tRNAOther) that functions to control expression TrpRS1 [7–9].
Sustaining charged tRNAs at levels adequate for the protein synthetic needs of growth under each environmental and nutritional condition is crucial for cell survival. Achieving this mandates that expression of each AARS be responsive to the cellular level of their charged cognate tRNAs. Therefore the mechanisms controlling AARS expression must be able to distinguish their cognate tRNA from other tRNA species and be able to measure the extent to which the pool of cognate tRNA is charged. Expression of the majority of AARSs in Bacillus subtilis is regulated by the T box antitermination mechanism . This mechanism was first discovered in studies on the regulation of threonyl- and tyrosyl-tRNA synthetase expression in B. subtilis[11–13], for a review see 14. The T box elements are widely distributed, being present in Firmicutes, δ-proteobacteria, Chloroflexi, Deinococcales/Thermales and Actinobacteria, and control expression of genes involved in cellular activities other than tRNA charging such as amino acid biosynthesis, amino acid transport and regulation of amino acid metabolism [15–17].
The T-box regulatory element is usually a 200-300 nucleotide untranslated RNA leader sequence containing a conserved T box sequence, stem-loop structures and a conditional Rho-independent terminator located upstream of the start codon [11–13]. Two specific interactions between tRNAs and T box leader sequences enable recognition of cognate tRNA species and distinction between charged and uncharged pools of tRNA. The NCCA sequence in the acceptor stem of a nonacylated-tRNA interacts with the UGGN sequence within the T box sequence (N varies according to the identity of the discriminator base of each tRNA) [13, 14, 18, 19]. This interaction cannot occur when a tRNA is aminoacylated, thereby distinguishing between charged and uncharged tRNAs. Specificity for cognate tRNAs is achieved by the presence of a specifier codon within a bulge in stem I of the leader sequence that interacts with the anticodon sequence of each tRNA. (eg. See Additional file 1, Figure S5). Thus for T box control of AARS expression, a high level of an uncharged tRNA (necessitating increased AARS production) causes interaction between that tRNA and its cognate T box element that stabilizes the anti-termination structure of the leader sequence allowing transcription of the AARS gene to proceed. A high level of aminoacylated-tRNAs in contrast cannot interact with the leader sequence allowing formation of the Rho-independent terminator and preventing continued transcription of the gene.
While most eubacteria encode either a class I or a class II LysRS, all sequenced strains of B. cereus (except strain AH820) and B. thuringiensis encode a copy of both enzyme types [8, 16, 17]. In Bacillus cereus strain 14579, the LysRS2-encoding lysS gene is positioned at the end of an operon encoding genes involved in folate metabolism, its normal position in most Bacilli while the lysK gene encoding the class I-type LysRS1 is located elsewhere on the chromosome. Shaul et al. (2006) show that this LysRS1 is closely related to the class I LysRS1 of Pyrococcus, suggesting that it has been acquired by B. cereus by horizontal transfer . The function of LysRS1 in B. cereus is not clear but it is expressed predominantly in stationary phase and can aminoacylate a novel tRNA species (tRNAOther) in concert with the class II LysRS enzyme . Thus it may play a role in surviving nutritional downshift in B. cereus.
Ataide and colleagues reported the presence of a putative T box regulatory element upstream of the lysK coding sequence in B. cereus strain 14579 . This was the first reported instance of putative control of LysRS expression by a T box mechanism. Here we investigate control of LysRS expression by a T box mechanism, confirming that it occurs only very rarely in bacteria. We show that the T box element of the lysK gene of B. cereus strain 14579 is functional and responds to an increased level of uncharged tRNALys in a canonical manner. Interestingly, this T box element shows some promiscuity in its specificity by responding to a reduced cellular level of asparaginyl-tRNAAsn. We also show that strains of B. subtilis, in which expression of the endogenous LysRS2 or the heterologous LysRS1 is controlled by this T box element, are viable.
Regulation of lysyl tRNA synthetase expression by a T-box antitermination mechanism occurs rarely
Occurrence of T box regulated lysyl-tRNA synthetase genes
T box regulated
B. cereus strains§
B. thuringiensis Konkukian
B. thuringiensis Al Hakam
The T-box element controlling expression of lysK in B. cereus strain 14579 is functional
A B. subtilis strain expressing the B. cereus class I LysK under T box regulatory control is viable
The rarity of the T box control of LysRS expression, and where found, occurs only in conjunction with a second cellular LysRS, prompted us to ask whether T box control of LysRS expression is compatible with viability. To address this question, B. subtilis strain NF54 (amyE:: PlysK(T box)lysK ∂lysS) was constructed in which expression of the B. cereus lysK gene is under the control of its natural promoter and T box regulatory element in single copy at the amyE locus and the endogenous lysS gene is partially deleted (373 amino acids of LysRS deleted leaving only the C-terminal 126 amino acids) by a double cross-over event. It is important to note that in strain NF54 the PlysK(T box)lysK cassette is flanked by transcriptional terminators, ensuring that lysK expression is solely dependent on the PlysK(T box)promoter. This strain was successfully constructed and verified by PCR and Southern blot analysis and by sequencing of selected regions (data not shown). This confirms that in B. subtilis T box mediated control of LysRS1 expression is compatible with viability. To establish whether NF54 has a phenotype, growth profiles were established in rich (LB) and minimal media and compared with wild-type B. subtilis strain 168. Results show an increase in the generation time of strain NF54 during growth in LB medium: NF54 has a doubling time of ~31 minutes while that of wild-type strain 168 is ~22 minutes under these conditions. However strain NF54 does not grow in minimal medium whereas wild-type strain 168 has a generation time of ~76 minutes in this medium. To establish whether this growth phenotype was due to reduced tRNALys charging, the PlysK(T box)lacZ was introduced into strain NF54 generating strain NF206. Reduced charging of tRNALys in strain NF206 will result in increased β-galactosidase accumulation when compared with strain BCJ363 that has the PlysK(T box)lacZ contruct in an otherwise wild-type background (ie. with the endogenous class II lysS). Results show that 250-300 units of β-galactosidase accumulate during exponential growth of strain NF206, an ~20-fold increase over that observed in the control strain BCJ363. We conclude T box control of LysR1 expression is compatible with viability of B. subtilis. However such strains have a reduced growth rate in rich medium and cannot be propagated in minimal medium probably due to reduced tRNALys charging.
A B. subtilis strain with expression of the endogenous class II lysS under the control of the T box regulatory element is viable and indistinguishable from wild-type in terms of growth and tRNALys charging
While T box control of LysRS1 expression supports growth of B. subtilis, the level of charged tRNALys is reduced and there is a growth phenotype. However it is unclear whether this phenotype is caused by T box regulation of LysRS expression or is due to the B. cereus derived class I LysRS1 enzyme that is reported to be less efficient catalytically than its class II counterpart . To distinguish between these possibilities and to further address the issue of T box regulation of LysRS, we constructed strain NF113 (lysS::PlysK(T box)lysS) that placed expression of the endogenous B. subtilis lysS gene under the control of the lysK promoter and T box element from B. cereus strain 14579. It is important to note that in strain NF113 the PlysK(T box)lysS cassette is flanked by transcriptional terminators ensuring that lysS expression is solely dependent on the PlysK(T box)promoter. Strain NF113 was successfully constructed and the relevant chromosomal regions verified by PCR and Southern blotting (data not shown) confirming that T box regulation of LysRS2 expression supports growth of B. subtilis. Importantly growth of strain NF113 in rich (LB) and minimal media (Spizizen salts) was indistinguishable from wild-type strain 168 (data not shown). The level of charged tRNALys was assessed in strain NF113 by introducing the PlysK(T box)lacZ transcriptional fusion to generate strain NF205. Approximately 10 units of β-galactosidase accumulated during exponential growth of strain NF205 similar to control strain BCJ363 (data not shown). We conclude that T box control of lysS expression is compatible with growth of B. subtilis. It is likely that the growth and tRNALys charging deficiency of strains NF54 and NF206 (containing T box regulated LysRS1) is caused by decreased efficiency of tRNALys charging by LysRS1 rather than by T box control of its expression.
The T box element associated with the B. cereus class I LysRS1 can be partially induced by asparagine starvation
We then sought to establish (i) if depletion of the cellular level of a charged tRNA leads to a general reduction in level of other charged tRNAs and (ii) if some level of cross-induction exists among T box elements controlling expression of AARS that charge the constituent tRNAs of mixed codon boxes in B. subtilis. To address both issues, transcriptional fusions of the promoter and T box element of the pheS, ileS and trpS AARS genes of B. subtilis with the lacZ reporter gene were constructed. Each fusion was introduced into strains auxotrophic for their cognate amino acids and into strains auxotrophic for the non-cognate amino acid in the mixed codon box. In each case, depletion for the cognate amino acid resulted in immediate induction of β-galactosidase expression while depletion for the non-cognate amino acid did not induce β-galactosidase expression to a significant level in any case (data not shown). These data show that depletion for an individual amino acid does not lead to a general increase in the level of uncharged tRNAs of other amino acids and that promiscuous cross-induction of T box controlled promoters by depletion of the non-cognate amino acid of a mixed codon box does not occur in B. subtilis.
We conclude that the T box element controlling expression of lysK encoding the class I LysRS1 of B. cereus strain 14579 displays some promiscuity of induction, being capable of responding to an increased level of uncharged tRNAAsn in addition to uncharged tRNALys. However such promiscuous cross-induction is not a general feature of T box elements in B. subtilis.
T box regulation of LysRS expression occurs rarely and in exceptional circumstances
The T box mechanism is widely employed to regulate AARS expression in a manner that is responsive to the level of uncharged cognate tRNA in the cell. Of 976 T box elements associated with regulation of AARS expression in 891 completely sequenced bacterial genomes identified in our analysis, potential T box control of LysRS expression was identified in only 4 bacterial species: T box elements were identified in all sequenced strains of B. cereus (except AH820) and B. thuringiensis, in association with a class I LysRS1 of Pyrococcal origin ; a T box element was identified in C. beijerinckii associated with a class II LysRS2  and a T box element was identified in S. thermophilum, associated with a class I LysRS1 . The T box elements in the Bacillus and Clostridium species are homologous: the T box elements of the Bacillus strains are ~92% identical while ~50% identity exists between the T box elements of the Bacillus and Clostridium species (see Additional file 1, Figure S1). However the T box element of S. thermophilum appears unrelated to the other T box elements (see Additional file 1, Figure S3). This is especially interesting since despite its high G+C (68.7%) content, S. thermophilum proteins are more similar to those of the low G+C Firmicutes such as Bacilli and Clostridia than to the high G+C Actinobacteria. In view of this, it is also interesting that among the homologous T box elements, those in the Bacilli are associated with a class I LysRS while the T box element in C. beijerinckii is associated with a class II LysRS. Thus T box regulation of LysRS expression appears to have evolved on two separate occasions, and one T box element has been conjoined with two different LysRS-encoding genes.
There are several interesting features about this cohort of T box regulated LysRS: (i) all bacterial species with a T box regulated LysRS have a second LysRS that is not T box regulated; (ii) the four T box elements in the phylogenetically related B. cereus and B. thuringiensis species are associated with a class I LysRS1 and display ~92% identity; (iii) the class I LysRS1 of B. cereus and B. thuringiensis is most closely related to LysRS1 from Pyrococcal species suggesting that a common ancestor of B. cereus/thuringiensis acquired it by a lateral gene transfer event ; (iv) the T box regulated LysRS1 in B. cereus strain 14579 is expressed predominantly in stationary phase  and (v) T box elements do not occur in Archaebacteria. The likely Pyrococcal origin of B. cereus LysRS1 and the absence of T box elements in Archaebacteria presents an interesting question as to how the regulatory sequence and structural gene were conjoined in this case. Perhaps tRNALys-responsive T box elements were more common in the ancestor of Firmicutes (supported by a similar T box element being associated with a class II LysRS2 in C. beijerinckii) and were selectively lost as controlling elements of the principal cellular LysRS, but were retained for control of ancillary LysRS enzyme expression. A second interesting possibility, especially in view of the fact that it can be induced by tRNAAsn, is that the T box element associated with lysK of B. cereus may have evolved from an element with a specificity determinant similar in sequence to that of lysine. These observations suggest that T box regulation may be unsuited for controlling expression of the housekeeping LysRS in bacteria and perhaps is only tolerated in additional copies of LysRS that play an ancillary role such as adaptation to stationary phase conditions as observed in B. cereus. Determining whether the other T box regulated lysS genes play an ancillary role requires further investigation. Notably, T box regulation of housekeeping aminoacyl tRNA synthetases is widespread, suggesting that it is some aspect of lysine metabolism that makes T box control of LysRS expression unsuitable as a regulatory mechanism.
The LysRS1 T box element from B. cereus is functional and B. subtilis strains with T box control of LysRS1 and LysR2 expression are viable
The unknown provenance and functionality of the T box element, despite the reported theoretical capability to form canonical T box element structures  prompted us to verify that it was functional and to ask whether strains of B. subtilis expressing a single copy of LysRS1 or LysRS2 controlled by this T box element are viable. We chose to conduct this study in B. subtilis because of the paucity of relevant auxotrophic B. cereus strains and other difficulties with antibiotic resistance and transformability. However we consider B. subtilis to be a valid model system in which to conduct this study. Our results show that the T box element is functional and can be induced up to 120-fold in response to lysine- or LysRS-depletion but not by depletion of non-cognate amino acids. Also strains of B. subtilis with expression of the endogenous LysRS2 controlled by this T box element are viable, and could not be distinguished from B. subtilis wild-type strain 168 during growth in rich or minimal medium. While a strain of B. subtilis expressing LysRS1 controlled by the T box element from B. cereus strain 14579 is also viable, it displays a growth defect when grown in rich medium and cannot be propagated in minimal medium. However it is likely that these phenotypes result from the reduced catalytic activity of class I LysRS enzyme rather than from control of expression by the T box element. These results show there is no a priori reason precluding control of LysRS expression by a tRNALys-responsive T box element. It emphasizes the puzzling rarity of T box regulated LysRS expression and the restriction of its occurrence in B. cereus strain 14579 to controlling expression of a LysRS1 enzyme that plays an ancillary role in adapting cells to adverse conditions.
The T box element controlling expression of LysRS1 in B. cereus strain 14579 can be induced by an increased level of uncharged tRNAAsn
The unusual occurrence of tRNALys-responsive T box elements and the experimentally demonstrated viability of B. subtilis strains with T box regulated LysRS expression prompted us to investigate why T box regulation of LysRS expression is rare. We noted a tendency in B. subtilis for non-T box regulated AARS (ArgRS, AsnRS, GltRS, LysRS, MetRS, and ProRS) to charge tRNAs with amino acids encoded in mixed codon boxes (ProRS being an exception, not being encoded by a mixed codon box). This observation, together with its possible origin being a T box element that is responsive to a different tRNA, prompted us to investigate whether the T box element controlling LysRS1 expression in B. cereus might also be induced by depletion of asparaginyl-tRNAAsn. Our results show that cellular depletion of AsnRS in B. subtilis results in induction of the PlysK(T box)lacZ. We show that this induction is not caused by concomitant depletion of lysyl-tRNALys since induction occurs when cellular levels of charged tRNALys are high (Figure 2). Importantly, there is no direct link in the biosynthetic pathways of lysine and asparagine. Also, expression of PlysK(T box)lacZ does not occur when cells are depleted for phenylalanine, showing that induction displays the expected specificity for lysine starvation. These data show that the T box element controlling expression of LysRS1 of B. cereus can be induced by an increased level of uncharged tRNALys and tRNAAsn. However such promiscuity of induction is restricted to this lysK-associated T box element since T box element control of expression of AARSs within mixed codon boxes is frequently found  and induction of the T box-controlled pheS, ileS and trpS genes was not observed in response to starvation for the non-cognate amino acid of the mixed codon box. The induction promiscuity of the B. cereus LysRS1-associated T box element might derive from its having evolved from a T box element that responded to a different tRNA. Such promiscuity may be tolerated since LysRS1 in B. cereus appears to have an ancillary role during stationary phase, or it may even be advantageous in that it makes LysRS1 expression responsive to a broader range of adverse nutritional conditions.
The T box regulatory element makes expression of AARS responsive to the uncharged level of their cognate tRNA and is widely used among bacteria. However significant variability exists in the frequency with which expression of individual AARSs is controlled by this mechanism [15–17], this study. It is largely unknown why T box regulation of LysRS expression is found in only 4 bacterial species (B. cereus, B. thuringiensis, S. thermophilum and C. beijerinckii) while more than 140 instances of T box control of IleRS expression are documented. Moreover these four bacterial species with a T box regulated LysRS all have a second non-T box regulated LysRS. We report that two tRNALys-responsive T box elements exist: the first is found in the Bacillus and Clostridium species controlling expression of a class I LysRS1 in Bacillus but a class II LysRS2 in Clostridium; the second in S. thermophilum displays little homology to the first T box element and controls expression of a class II LysRS2. We established that the T box element associated with lysK expression in B. cereus strain 14579 is functional, but unusually responds to an increased level of uncharged tRNALys and tRNAAsn. Since LysRS1 is expressed mainly in stationary phase, this unusual induction profile may make its expression responsive to a wider range of nutritional signals. We also demonstrated that B. subtilis cells, in which expression of the endogenous lysS is controlled by the lysK T box element from B. cereus, are viable and are indistinguishable from wild-type B. subtilis strain 168 in terms of growth and tRNALys charging. Thus there appears to be no a priori reason why expression of the main cellular LysRS is not regulated by a T box element in B. subtilis (in fact expression of a majority of the AARS are T box regulated in B. subtilis), making the rarity of T box control of LysRS expression among bacteria even more puzzling.
Bacterial strains, media and growth conditions
Bacterial strains and plasmids used in this work
Reference or source
supE hsd Δ5 thi Δ(lac-proAB) F'[traD 36]
recBC hsdR - M - cyab10pcn
B. cereus 14579
wild type isolate
trpC2 lys amyE::pBCJ307 (PlysK (T box)lacZ) CmR
trpC2 amyE::pNF48 (PlysK (T box)lysK) SpecR
trpC2 amyE::pNF48 (PlysK (T box)lysK) SpecR DlysS KanR
trpC2 amyE::pBCJ307 (PlysK (T box)lacZ) CmR asnS::pNF40 (Pspac asnS) EmR
trpC2 amyE::pBCJ307 (PlysK (T box)lacZ) CmR asnS::pNF40 (Pspac asnS) EmRpMap65 (P pe -lacI) PhR
trpC2 lysS::pNF112 (PlysK (T box)lysS) CmR
trpC2 amyE:: pBCJ307 (PlysK (T box)lacZ) EmR CmR
trpC2 lysS::pNF112 (PlysK (T box)lysS) CmR amyE:: pBCJ307(PlysK (T box)lacZ) EmR
trpC2 amyE::pNF48 (PlysK (T box)lysK) SpecR DlysS: KanR amyE::pBCJ307(PlysK (T box)lacZ)CmR
trpC2 amyE::pBCJ307 (PlysK (T box)lacZ) CmR
trpC2 amyE::pBCJ307 (PlysK (T box)lacZ) CmR lys::pXZ2 (Pspac lysS) EmR
trpC2 amyE::pBCJ307 (PlysK (T box)lacZ) CmR lys::pXZ2 (Pspac-lysS) EmR pMap65 PhlR
cloning vector ApR
Stratagene, La Jolla CA
integration vector for B. subtilis
pBluescript based vector containing transcription terminator cassettes ApR
vector to replace part of B. subtilis lysS with KanR
vector with transcriptional fusion of B. cereus lysK promoter and T box with lacZ CmR
vector to generate lacZ promoter fusions at the amyE locus by double crossover ApR CmR
vector for integration at the amyE locus in B. subtilis SpecR
Vector to placing B. subtilis lysS under control of IPTG inducible Pspac promoter EmR
pMUTIN4 with the lacZ gene removed EmR
replicating B. subtilis plasmid encoding penP-lacI PhlR KanR
plasmid with B. cereus lysK promoter and T box element in pBCJ102 ApR
B. subtilis asnS promoter on 516 bp fragment in pMUTIN-XZ
B. cereus lysK promoter and lysK gene cloned into pDG1730
the lysK promoter and T box element (423 bp) fused to B. subtilis lysS (672 bp fragment)
General molecular biology methods
Oligonucleotides used in this study
To construct strain NF33, a 400 bp DNA fragment from the region upstream of B. cereus lysK was amplified by PCR using primers NF36F and NF36R, cut with Eco RI and Bam HI, and ligated into similarly restricted pDG268  to produce the plasmid pBCJ307. pBCJ307 was inserted into the amyE locus of the B. subtilis lysine auxotroph strain 1A765 by double crossover to produce strain NF33.
In order to analyze the effect of a reduction of the cellular level of charged tRNALys on expression of a PlysK(T box)lacZ fusion, strain BCJ367 was constructed. Plasmid pBCJ307 was integrated into the B. subtilis chromosome by a double crossover event at the amyE locus to produce strain BCJ363. To place the endogenous lysS gene of B. subtilis under IPTG inducible control, plasmid pMUTIN4  was digested with Sal I and Bsi WI and eluted from an agarose gel to remove the 2 kb lacZ gene. The ends of the plasmid molecule were blunt ended using Klenow polymerase and religated, resulting in plasmid pMUTINXZ. A 670 bp DNA fragment encoding the end of the yacF gene was amplified with oligonucleotides NF2F and NF2R using B. subtiliis strain 168 chromosomal DNA as a template. This fragment was digested with Eco RI and inserted into the Eco RI site of pMUTINXZ, resulting in plasmid pXZ2. Plasmid pXZ2 was then integrated onto the chromosome of strain BCJ363 by a Campbell type event generating strain BCJ366 thereby placing expression of the lysS gene under the control of the IPTG inducible Pspac promoter. To effect tight control of the Pspac promoter, replicating plasmid pMap65  that encodes a lacI gene, was transformed into BCJ366 to produce strain BCJ367.
Strain NF54 was made to assess whether a B. subtilis strain expressing a T-box regulated lysK gene was viable. A 1.95 kb fragment of the B. cereus chromosome encoding the lysK promoter, leader region and structural gene was generated by PCR using oligonucleotides NF36F and NF9R. This fragment was digested with Eco RI and cloned into the Eco RI site of plasmid pBCJ102 that has transcriptional terminators flanking the multiple cloning site, to generate plasmid pNF30 . A 2567 bp fragment encoding the lysK promoter, T box element and structural gene flanked by transcriptional terminator sequences was amplified using the pBluescript T7 and M13 reverse primers and plasmid pBCJ102 as template. The ends of this fragment were phosphoryalted using T4 polynucleotide kinase (Promega) and it was then cloned into the Eco RV site of plasmid pDG1730  to produce the plasmid pNF48. Plasmid pNF48 was integrated at the amyE locus of the B. subtilis chromosome by a double crossover event to produce strain NF52. The lysS gene of strain NF52 was then partially deleted by integration of plasmid pBCJ144  into the chromosome by a double-crossover event, replacing part of lysS with a kanamycin resistance cassette, thereby generating strain NF54. In strain NF54, 373 amino acids of LysRS are deleted leaving only the C-terminal 126 amino acids). Importantly, in this strain the PlysK (Tbox)lysK construct is flanked by transcriptional terminators so that lysK expression is solely dependent on the PlysK (Tbox)promoter. To insert the PlysK (Tbox)lacZ reporter fusion into the chromosome of B. subtilis strain NF54, plasmid pBCJ307 was integrated at the amyE locus, thereby generating strain NF206.
To construct B. subtilis strain NF113, that has expression of the endogenous lysS gene under the control of the lysK promoter and T box element, a 423 bp DNA fragment encoding the B. cereus lysK promoter and T box element (generated using oligonucleotides NF36F and NF15R) was fused to a 672 bp fragment of the lysS gene (generated using oligonucleotides NF15F and NF3R/2) by overlapping PCR (using the outside primers NF36F and NF3R/2). This DNA fragment was then digested with Eco RI and Bam HI and cloned into Eco RI digested pBCJ102  to generate the plasmid pNF112: the PlysK (Tbox)lysS insert is flanked by transcriptional terminators in this plasmid. Plasmid pNF112 was then integrated into the B. subtilis chromosome at the lysS locus by a Campbell-type event to produce the strain NF113. To introduce the PlysK (Tbox)lacZ reporter fusion into strain NF113, it was transformed with chromosomal DNA from strain NF204 that contains the PlysK (Tbox)lacZ reporter fusion at the amyE locus, thereby generating strain NF205. Strain NF204 was constructed by transformation of strain 1A717  with pBCJ307. To construct B. subtilis strain NF60 in which expression of the endogenous asnS gene is placed under the control of the IPTG-dependent PSpac promoter and containing the PlysK(T box)lacZ fusion, a 516 bp DNA fragment encoding the asnS promoter region was amplified using oligonucleotides NF16F and NF16R, digested with Hind III and cloned into Hind III digested pMutinXZ to produce plasmid pNF40. Plasmid pNF40 was transformed into B. subtilis strain BCJ363 by a Campbell-type event to produce strain NF58. Plasmid pMAP65 (encoding the lacI gene) was then established in strain NF58 to ensure strict IPTG-dependent asnS expression, thereby generating strain NF60.
Measurement of tRNA charging by Northern analysis
Establishing the level of charged tRNALys was carried out as previously described . B. subtilis tRNALys was detected with an oligonucleotide probe complementary to nucleotides 26-51 that was labeled either with DIG oligonucleotide Tailing Kit (Roche, East Sussex, UK) or with biotin (New England Biolabs, USA). Detection used either the DIG labeling kit (Roche, East Sussex, UK) or the NEB blot phototope kit (New England Biolabs, USA) according to the manufacturer's instructions.
Determination of β-galactosidase activity
Measurement of β-galactosidase activity was as previously described .
The genes encoding AARS proteins were identified in 891 bacterial genomes by homology with their orthologues of B. subtilis - for glutaminyl tRNA synthetases, the E. coli protein was used. Only proteins that displayed BLAST E-values of less than 10-10 were retained for further analysis. The complete upstream region of each AARS-encoding gene was examined for the presence of the T-box motif TGGNACCGCG, allowing up to two mismatches in the last six positions. Sequences containing potential T-box sequences were then examined manually for their ability to form mutually exclusive terminator and anti-terminator DNA structures
This work was supported by Science Foundation Ireland Principal Investigator Awards (03/IN3/B409 and 08/IN.1/B1859) and by the EU Sixth Framework grant BACELL Health (LSHC-CT-2004-503468).
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